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    INTERNATIONAL PROGRAMME ON CHEMICAL SAFETY


    ENVIRONMENTAL HEALTH CRITERIA 2





    POLYCHLORINATED BIPHENYLS AND TERPHENYLS






    This report contains the collective views of an international group of
    experts and does not necessarily represent the decisions or the stated
    policy of either the World Health Organization or the United Nations
    Environment Programme.

    Published under the joint sponsorship of 
    the United Nations Environment Programme
    and the World Health Organization

    World Health Organization
    Geneva, 1976

    ISBN 92 4 154062 1

    (c) World Health Organization 1976

        Publications of the World Health Organization enjoy copyright
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    Universal Copyright Convention. For rights of reproduction or
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    should be made to the Office of Publications, World Health
    Organization, Geneva, Switzerland. The World Health Organization
    welcomes such applications.

        The designations employed of any opinion of the material in this
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    concerning the legal status of any country, territory, city or area or
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        The mention of specific companies or of certain manufacturers'
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    proprietary products are distinguished by initial capital letters.

    CONTENTS

    ENVIRONMENTAL HEALTH CRITERIA FOR POLYCHLORINATED BIPHENYLS AND
    TERPHENYLS

    1. SUMMARY AND RECOMMENDATIONS FOR FURTHER RESEARCH
         1.1. Introductory note
         1.2. Summary
                1.2.1. Composition and analytical problems
                1.2.2. Sources and pathways in the environment
                1.2.3. Concentration in the environment
                1.2.4. Metabolism
                1.2.5. The extent of human exposure
                1.2.6. Experimental studies on the effects of PCBs and
                         PCTs
                1.2.7. Clinical studies of the effects of PCBs in man
                1.2.8. Dose-effect relationships
         1.3. Recommendations for further research
                1.3.1. Analytical methods
                1.3.2. Environmental pollution
                1.3.3. Effects on man
                1.3.4. Experimental studies
                1.3.5. PCB substitutes

    2. PROPERTIES AND ANALYTICAL METHODS
         2.1. Chemical composition
         2.2. Purity of products
         2.3. Determination of PCB residues
                2.3.1. Extraction of sample
                2.3.2. Clean-up
                2.3.3. Chromatographic separation of PCBs
                2.3.4. Quantification of PCB content
                2.3.5. Accuracy of PCB determination
                2.3.6. Confirmation of identity
         2.4. Determination of PCTs

    3. SOURCES OF ENVIRONMENTAL POLLUTION
         3.1. Production and uses of PCBs
         3.2. Entry of PCBs into the environment
                3.2.1. Release of PCBs into the atmosphere
                3.2.2. Leakage and disposal of PCBs in industry

    4. ENVIRONMENTAL TRANSPORT AND TRANSFORMATION
         4.1. Environmental transport
                4.1.1. Air transport
                4.1.2. Transport in soil
                4.1.3. Transport in water
                4.1.4. Transport through biota

         4.2. Transformation in the environment
                4.2.1. Abiotic transformation
                4.2.2. Biotransformation
                4.2.3. Metabolism in limited ecosystems
         4.3. Biological accumulation

    5. ENVIRONMENTAL LEVELS AND EXPOSURES
         5.1. Air
         5.2. Soil and sediments
         5.3. Water
         5.4. Living organisms
                5.4.1. The influence of local pollution
                5.4.2. The influence of fat content of tissues
                5.4.3. The influence of the trophic stage in food chains
                5.4.4. Indicator organisms
         5.5. The extent of human exposure to PCBs and PCTs
                5.5.1. Air and water
                5.5.2. Food
                5.5.3. Occupational exposure
                5.5.4. Other sources of exposure
                5.5.5. Biological indices of human exposure
                         5.5.5.1    Body fat
                         5.5.5.2    Blood
                         5.5.5.3    Human milk
                5.5.6. Estimated daily intake

    6. METABOLISM
         6.1. Absorption
         6.2. Tissue distribution of PCBs
         6.3. Tissue distribution of PCTs
         6.4. Placental transport
         6.5. Excretion and elimination
                6.5.1. Milk
                6.5.2. Eggs
                6.5.3. Urine and faeces
         6.6. Biotransformation
                6.6.1. Metabolic degradation
                6.6.2. The effect of structure on retention

    7. EXPERIMENTAL STUDIES ON THE EFFECTS OF PCBs AND PCTs
         7.1. Toxic effects in different species
                7.1.1. Mammals
                         7.1.1.1    Acute oral and intravenous toxicity
                         7.1.1.2    Subacute oral toxicity
                         7.1.1.3    Chronic oral toxicity
                         7.1.1.4    Dermal toxicity
                         7.1.1.5    Inhalation toxicity
                7.1.2. Birds

                7.1.3. Aquatic organisms
                         7.1.3.1    Fish
                         7.1.3.2    Aquatic invertebrates
                         7.1.3.3    Microorganisms
         7.2. Toxicity of impurities in commercial PCBs
         7.3. Toxicity of the PCTs
         7.4. Biochemical effects
                7.4.1. Induction of enzymes
                7.4.2. Porphyria
                7.4.3. Effects on steroid metabolism
                7.4.4. Other biochemical effects
                7.4.5. Potentiation and antagonism by PCBs
         7.5. Cytotoxic effects
         7.6. Immunosuppressive effects
         7.7. Effects on reproduction
         7.8. Neoplasia and adenofibrosis

    8. EFFECTS OF PCBs AND PCTs ON MAN -- EPIDEMIOLOGICAL AND CLINICAL
         STUDIES

    9. EVALUATION OF HEALTH RISKS TO MAN FROM EXPOSURE TO PCBs AND PCTs
         9.1. Species variation
         9.2. Dose-effect relationships
                9.2.1. Body weight
                9.2.2. Effects on liver
                9.2.3. Reproduction
                9.2.4. Immunosuppression
                9.2.5. Skin effects
         9.3. Nondetected effect levels

    REFERENCES
    

    NOTE TO READERS OF THE CRITERIA DOCUMENTS

        While every effort has been made to present information in
    criteria documents as accurately as possible without unduly delaying
    publication, mistakes might have occurred and are likely to occur in
    the future. In the interest of all users of the environmental health
    criteria documents, readers are kindly requested to communicate any
    errors found to the Division of Environmental Health, World Health
    Organization, Geneva, Switzerland, in order that they may be included
    in corrigenda which will appear in subsequent volumes.

        In addition, experts in any particular field dealt with in the
    criteria documents are kindly requested to make available to the WHO
    Secretariat any important published information that may have
    inadvertently been omitted and which may change the evaluation of
    health risks from exposure to the environmental agent under
    examination, so that information may be considered in the event of
    updating and re-evaluating the conclusions contained in the criteria
    documents.

    WHO TASK GROUP ON ENVIRONMENTAL HEALTH CRITERIA FOR POLYCHLORINATED
    BIPHENYLS AND TERPHENYLS

     Copenhagen, 20-24 October 1975

    Participants

     Members

    Dr. V. Benes, Department of Toxicology, Institute of Hygiene and
        Epidemiology, Prague, Czechoslovakia  (Vice-Chairman)

    Dr. H. L Falk, National Institute for Environment Health Services,
        Research Triangle Park, NC, USA

    Mr L. Gordts, Institute of Hygiene and Epidemiology, Brussels, Belgium

    Dr. D. L. Grant, Pesticides Section, Toxicology Evaluation Division,
        Bureau of Chemical Safety, Department of Health and Welfare,
        Ottawa, Ontario, Canada  (Rapporteur)

    Mr A. V. Holden, Department of Agriculture and Fisheries for Scotland,
        Freshwater Fisheries Laboratory, Faskally, Pitlochry, Perthshire,
        Scotland

    Dr. S. Jensen, Naturvardsverkets Specialanalytiska Laboratorium,
        Wallenberg-laboratoriet, Lilla Frescati, Stockholm, Sweden

    Dr. Renate Kimbrough, Center for Disease Control, Toxicology Branch,
        Atlanta, GA, USA

    Professor H. Kuratsune, Department of Public Health, Faculty of
        Medicine, Kyushu University, Fukuoka, Japan

    Dr. E. Schulte, Institut fur Lebensmittelchemie der Westfälischen
        Wilhelms-Universität, Munster/Westf., Federal Republic of Germany

    Dr. J. G. Vos, Laboratory for Pathology, National Institute for Public
        Health, Bilthoven, Netherlands  (Chairman)

     Observer

    Dr. D. Axelrod, Division of Laboratories and Research, New York State
        Department of Health, Albany, NY, USA

              

    Unable to attend

    a  Dr M. V. Kryznovskaja, All-Union Institute for Research on
       Hygiene and Toxicology of Pesticides, Polymers, and Plastics, Kiev,
       USSR.

     Representatives of other organizations:

     International Federation of Pharmaceutical Manufacturers Associations
        Professor P. Fabiani, Laboratoire du Chimie et de Toxicologie de
        l'Hōtel Die, Paris, France

     Permanent Commission and International Association on Occupational
         Health
        Dr Aa. Grut, State Labour Inspection Service, Hellerup, Denmark

     Secretariat:

    Dr J. C. Gage, 21 Lambolle Road, London, England  (Temporary Adviser)

    Dr. M. J. Suess, Environmental Pollution Control, WHO Regional Office
        for Copenhagen, Denmark

    Dr A. H. Wahba, Health Laboratory Services, WHO Regional Office for
        Europe, Copenhagen, Denmark

    Dr. G. Vettorazzi, Food Additives Unit, Division of Environmental
        Health, World Health Organization, Geneva  (Secretary)

    Dr. D.C. Villeneuve,a Laboratory of Toxicology, National Institute of
        Public Health, Bilthoven, Netherlands  (Temporary Adviser)

              

    a  On sabbatical leave from: Biochemical Toxicology Unit,
       Environmental Toxicology Division, Environmental Health Centre,
       Department of Health and Welfare, Ottawa, Ontario, Canada.

    ENVIRONMENTAL HEALTH CRITERIA FOR POLYCHLORINATED BIPHENYLS AND
    TERPHENYLS

        A WHO Task Group on Environmental Health Criteria for
    Polychlorinated Biphenyls (PCBs) and Terphenyls (PCTs) met in
    Copenhagen from 20-24 October 1975. Dr F. A. Bauhofer, Director of
    Health Services of the WHO Regional Office for Europe opened the
    meeting on behalf of the Director-General and the Director of the
    Regional Office for Europe. The Task Group reviewed and amended the
    second draft criteria document and made an evaluation of the health
    risks from exposure to these compounds.

        The preparation of the first draft criteria document was based on
    national reviews of health effects research on polychlorinated
    biphenyls, received from the national focal points collaborating in
    the WHO Environmental Health Criteria Programme in Canada, the Federal
    Republic of Germany, Finland, France, Japan, the Netherlands, New
    Zealand, Sweden, the United Kingdom, and the USA. Dr J. C. Gage,
    London, England, prepared the first draft as well as the second draft
    criteria document which took into account the comments received from
    the national focal points in Canada, Czechoslovakia, the Federal
    Republic of Germany, France, Japan, New Zealand, Sweden, the United
    Kingdom, the USA, and the USSR; from the United Nations Industrial
    Development Organization (UNIDO), Vienna, the Food and Agriculture
    Organization of the United Nations (FAO) Rome, and from the United
    Nations Educational, Scientific and Cultural Organization (UNESCO)
    Paris; the Organization for Economic Co-operation and Development,
    Paris, and the Health Protection Directorate of the Commission of the
    European Communities, Luxembourg.

        Comments were also received at the request of the Secretariat,
    from Dr K. Kojima, Japan, Dr D. S. May, United Kingdom, and Dr V.
    Zitko, Canada.

        The collaboration of these national institutions, international
    organizations and individual experts is gratefully acknowledged.
    Without their assistance the document could not have been completed.

        This document is based primarily on original publications listed
    in the reference section. In addition, some recent publications
    reviewing the environmental and health aspects of polychlorinated
    biphenyls were also used. These include reviews by the Commission of
    the European Communities (1974), the US Department of Health,
    Education and Welfare (1972), the International Agency for Research on
    Cancer (1974), the International Council for the Exploration of the
    Sea (1974), Jensen (1974), Kimbrough (1974), the National Swedish
    Environment Protection Board (1973), the Panel on Hazardous Trace
    Substances (1972), the USDA/USDC/EPA/FDA/USDA (1972), and a WHO
    working group (1973).

        Details about the WHO Environmental Health Criteria Programme
    including the definition of some terms frequently used in the document
    may be found in the general introduction to the Environmental Health
    Criteria Programme published together with the Environmental Health
    Criteria Document on mercury (Environmental Health Criteria 1, Geneva
    World Health Organization, 1976).

    1.  SUMMARY AND RECOMMENDATIONS FOR FURTHER RESEARCH

    1.1  Introductory Note

        The commercial production of the polychlorinated biphenyls (PCBs)
    began in 1930, and during the 1930s cases of poisoning were reported
    among men engaged in their manufacture. The nature of this
    occupational disease was characterized by a skin affliction with
    acneiform eruptions; occasionally the liver was involved, in some
    cases with fatal consequences. Subsequent safety precautions appear
    largely to have prevented further outbreaks of this disease in
    connection with the manufacture of PCBs, but from 1953 onwards, cases
    have been reported in Japanese factories manufacturing condensers.

        The distribution of PCBs in the environment was not recognized
    until Jensen started an investigation in 1964 to ascertain the origins
    of unknown peaks observed during the gas-liquid chromatographic
    separation of organochlorine pesticides from wild-life samples. In
    1966, he and his colleagues succeeded in attributing these to the
    presence of PCBs. Since that time, investigations in many parts of the
    world have revealed the widespread distribution of PCBs in
    environmental samples.

        The serious outbreaks of poisoning in man and in domestic animals
    from the ingestion of food accidentally contaminated with PCBs have
    stimulated investigations into the toxic effects of PCBs on animals
    and on nutritional food chains. This has resulted in limitation of the
    commercial exploitation of PCBs and polychlorinated terphenyls (PCTs),
    and in regulations to limit the residues in human and animal food.

        The environmental impact of the PCBs and PCTs has been the subject
    of several reviews, and has been discussed at a number of regional and
    international meetings. The relevant publications are mentioned in the
    previous section.

    1.2  Summary

    1.2.1  Composition and analytical problems

        The commercial production of PCBs and PCTs by the direct
    chlorination of biphenyl and terphenyl leads to a mixture of
    components with a range of chlorine contents, the mean percentage
    chlorine in the product being controlled to give the required
    technical properties. Most of these components have been separated by
    gas-liquid chromatography, and the PCBs in the mixtures have been
    characterized after synthesis of the components by unequivocal routes.
    Techniques are available to analyse environmental samples for PCBs and
    PCTs, and experience has that interlaboratory collaborative studies
    are necessary to competence to determine residues below the 1 mg/kg
    level.

        The commercial PCB mixtures contain various quantities of
    impurities among which chlorinated dibenzofurans and chlorinated
    naphthalenes have been identified.

    1.2.2  Sources and pathways in the environment

        The estimated cumulative world production of PCBs since 1930 is of
    the order of 1 million tonnes. Of this, more than one-half has entered
    dumps and landfills, where it is likely to be stable and released only
    very slowly. Much of the remainder has entered the environment by the
    disposal of industrial fluids into rivers and coastal waters, by
    leakage from nonenclosed systems, or by volatilization into the
    atmosphere from incineration of PCB-containing material at dumps. The
    ultimate reservoirs of PCBs and PCTs that enter the environment are
    mainly sediments of rivers and coastal waters. PCBs and PCTs are
    stable in the environment, but a small proportion is transformed by
    biological action and possibly by photolysis.

    1.2.3  Concentration in the environment

        Measured concentrations of PCBs in air range from 50 ng/m3 to
    less than 1 ng/m3. Nonpolluted fresh waters should contain less than
    0.5 ng of PCBs per litre compared with 50 ng per litre in moderately
    polluted rivers and estuaries, and 500 ng per litre in highly polluted
    rivers. The concentration in living organisms depends upon the extent
    of local pollution, the amount of fat in the tissues, and the trophic
    stage of the organism in food chains. Highest tissue levels were found
    in marine ecosystems with very high values in top predators from
    polluted areas though most of the fish caught for human consumption
    contains PCBs at levels of less than 0.1 mg/kg in muscle tissue. There
    is no information available on the environmental distribution of PCTs.

    1.2.4  Metabolism

        PCBs are well absorbed by mammals through the gastrointestinal
    tract, lungs, and skin. They are stored mainly in adipose tissue and
    there is some placental transfer. Excretion in mammals is mainly
    through faeces, where the PCBs appear as phenolic metabolites; they
    appear unchanged in human milk. In birds, there is a considerable
    excretion in eggs. The rate of excretion in faeces is dependent on the
    rate of metabolism and this is much influenced by the number and
    orientation of the chlorine substituents. As environmental PCBs pass
    up biological food chains, there is a progressive loss of the lower
    chlorinated components owing to selective biotransformation, and only
    traces of PCBs containing less than five chlorine atoms per molecule
    are found in human fat.

        The smaller amount of information available concerning the PCTs
    indicates that they also are absorbed from the gastrointestinal tract
    and undergo selective biotransformation, but that the concentration in
    fat in relation to that in other tissues appears to be less than is
    observed with the PCBs.

    1.2.5  The extent of human exposure

        Surveys on human adipose tissue in several countries have shown
    that most samples contain levels of PCBs in the region of 1 mg/kg or
    less, although higher values have been reported from some countries.
    Much higher values, up to 700 mg/kg, have been found in fat from men
    occupationally exposed. Several national surveys give PCB
    concentrations in the blood in the region of 0.3 µg/100 ml but levels
    approaching 200 µg/100 ml have been measured in men occupationally
    exposed, and these may be associated with skin lesions. Most surveys
    on human milk have shown PCB concentrations in the region of
    0.02 mg/litre, although concentrations up to 0.1 mg/litre have been
    recorded. Results from the very few investigations on PCT
    concentrations in fat and blood suggest that these may be equal to
    those of PCBs.

        An estimate of the total daily intake of PCBs in air, water, and
    diet by individuals not occupationally exposed indicates that this
    falls within the range of 5-100 µg, which may be supplemented by
    unknown amounts from nondietary sources. This estimate has some
    support from measurements of concentrations in human milk.

    1.2.6  Experimental studies on the effects of PCBs and PCTs

        Most of the studies on the toxicity of PCBs have been performed
    with the commercial mixtures. The PCBs are of low acute toxicity but
    the effects are cumulative with prolonged administration; in mammals,
    liver enlargement is observed that may progress to liver damage.
    Non-metastasizing neoplastic liver nodules have been produced in rats
    and mice, some of which were classified as hepatocellular carcinomas
    on the basis of histological criteria in one study in rats and one
    study in mice. The monkey is much more sensitive to PCBs than the rat,
    showing effects similar to those seen in human Yusho patients (See
    section 8, p 65) with a similar order of exposure. Low dose effects on
    fertility have been seen in both the monkey and the mink, a species
    that is also relatively sensitive to PCBs.

        Other effects of PCBs include porphyria, immunosuppression, and
    interference with steroid metabolism; some of these maybe attributable
    to the increase in microsomal enzyme activity associated with liver
    enlargement. Some of the toxic effects can be attributed to impurities
    in the commercial products.

        The toxicity of PCBs to fish is not high by comparison with that
    of some pesticides, but some aquatic invertebrates are more sensitive.
    There is little information on the toxicity of the PCTs.

    1.2.7  Clinical studies of the effects of PCBs in man

        Information on the effects of PCBs in man has been obtained from a
    large-scale incident in Japan (Yusho), in which over 1000 individuals
    showed signs of poisoning from the ingestion of rice oil contaminated
    with PCBs from a heat exchanger liquid. The most striking effects were
    hypersecretion in the eyes, pigmentation and acneiform eruptions of
    the skin, and disturbances of the respiratory system. Babies born to
    Yusho mothers were of less than normal size and initially showed skin
    pigmentation. Over a six-year period, the effects on the skin
    diminished very gradually, but the nonspecific symptoms tended to
    become somewhat more prominent. The smallest dose of PCBs calculated
    to produce an effect was approximately 0.5 g over about 120 days, but
    as the rice oil contained chlorinated dibenzofurans at a concentration
    of 5 mg/kg of rice oil in addition to PCBs at 2000-3000 mg/kg it is
    not certain that the symptoms were due solely to PCBs.

    1.2.8  Dose effect relationships

        Experimental studies on the dose-effect relationship have shown
    that no effects on growth, and reproduction are seen in rats receiving
    PCB levels of 1 mg/kg body weight per day; there may be liver
    enlargement and a reversible induction of microsome enzyme activity at
    a level of 1 mg/kg/day but not at 0.1 mg/kg/day. Effects on
    reproduction are seen in the monkey with PCB levels of about
    0.12 mg/kg/day. Symptoms were reported in some Yusho patients
    ingesting less than 0.1 mg/kg/day.

    1.3  Recommendations for Further Research

    1.3.1  Analytical methods

        Collaborative intercalibration studies on the determination of
    PCBs, PCTs, and chlorinated dibenzofurans should be established
    between all laboratories engaged in determining these compounds in
    environmental samples, and adequate standards should be made available
    for individual chlorinated biphenyls and dibenzofurans.

        Improved analytical techniques, including those involving
    capillary gas-liquid chromatography and mass spectroscopy, should be
    developed for the determination of PCBs, PCTs, polybrominated
    biphenyls, polychlorinated dibenzofurans, and naphthalenes, and their
    metabolites and degradation products.

    1.3.2  Environmental pollution

        The content of chlorinated dibenzofurans should be studied in a
    range of commercial PCB mixtures, and in used PCBs from existing or
    newly designed heat exchangers, capacitors, and hydraulic
    transmissions. The possibility of the formation of chlorinated
    dibenzofurans from PCBs in cooking oils before and after use and in
    other foods during storage or heating requires investigation.

        The current production, use patterns, and methods of disposal of
    PCBs should be carefully examined to gain information on the impact of
    PCBs on the environment at the present time. The rate of leaching of
    PCBs from waste dumps and landfills should be studied, and methods of
    incineration should be investigated to ascertain which of the
    components survive inefficient combustion, and whether chlorinated
    dibenzofurans or other compounds are released into the atmosphere.

        Information is required on the metabolism and environmental fate
    of the chlorinated dibenzofurans.

    1.3.3  Effects on man

        The intake of PCBs and PCTs from all sources by typical
    populations should be studied, and an attempt should be made to trace
    the sources of PCBs and PCTs in those items of the diet that make the
    greatest contribution to the daily intake. Further measurements are
    required on levels in human body fat, blood, and milk, and an attempt
    should be made to relate these levels to the daily intake.

        Clinical and epidemiological studies are required on individuals
    exposed to relatively high concentrations of PCBs and PCTs, either
    occupationally or by virtue of the nature of their diet, and their
    status should be correlated with exposure and tissue levels.

    1.3.4  Experimental studies

        Further toxicological and metabolic studies are required on
    polychlorinated biphenyls, dibenzofurans, naphthalenes, and impurities
    occurring in commercial products, on a variety of species including
    primates, in order to assess the nature of the toxic effects, the
    dose-response relationship, and the threshold of toxic effects. Such
    investigations should be extended to include dermal and inhalation
    exposure.

        Carcinogenic and cocarcinogenic studies should be undertaken to
    identify the components in commercial PCBs responsible for neoplastic
    effects.

    1.3.5  PCB substitutes

        More information should be made available on the production and
    use patterns of PCTs, polybrominated biphenyls, and of other possible
    substitutes for PCBs, and when appropriate, these products should be
    subjected to adequate toxicological investigations.

    2.  PROPERTIES AND ANALYTICAL METHODS

    2.1  Chemical Composition

        The PCBs form a class of chlorinated hydrocarbons and are
    manufactured commercially by the progressive chlorination of biphenyl
    in the presence of a suitable catalyst. They are known by a variety of
    trade names: Aroclor (USA), Phenochlor (France), Clophen (Federal
    Republic of Germany), Kanechlor (Japan), Fenchlor (Italy), and Sovol
    (USSR). Their value for industrial applications depends upon their
    chemical inertness, resistance to heat, non-flammability, low vapour
    pressure (particularly with the higher chlorinated compounds), and
    high dielectric constant. There are many different trade names for
    mixtures of PCBs with other compounds.

        Individual manufacturers have their own system of identification
    for their products. In the Aroclor series, a four digit code is used;
    biphenyls are generally indicated by 12 in the first two positions,
    while the last two numbers indicate the percentage by weight of
    chlorine in the mixture; thus Aroclor 1260 is a polychlorinated-
    biphenyl mixture containing 60% of chlorine. An exception to this
    generalization is Aroclor 1016 which is a distillation product of
    Aroclor 1242 containing only 60% of components with five or more
    chlorine atoms (Burse et al, 1974). With other commercial products the
    codes may indicate the approximate mean lumber of chlorine atoms in
    the components; thus Clophen A60, Pheno-DP6, and Kanechlor 600 are
    biphenyls with an average of about six chlorine atoms per molecule
    (equivalent to 59.0% chlorine by weight).

        In the Aroclor series, terphenyls are indicated by 54 in the first
    two of the four digit code. In Japan, the PCTs are coded Kanechlor.

        Individual PCBs have been synthesized for use as reference samples
    in the identification of gas-liquid chromatographic peaks, for
    toxicological investigations, and for studying their metabolic fate in
    living organisms, for which purpose they have been prepared labelled
    with carbon-14 (Hutzinger et al., 1971; Tas & de Vos, 1971; Webb &
    McCall, 1972; Moron, et al., 1972; Sundström & Wachtmeister, 1973;
    Jensen & Sundström, 1974).

        The chlorination of biphenyl can lead to the replacement of 1-10
    hydrogen atoms by chlorine; the conventional numbering of substituent
    positions is shown in the diagram. It has been calculated that 210
    different biphenyls of different chlorine content are theoretically
    possible, although Sissons & Welti (1971) have demonstrated that
    chlorine substituents in the 3,5- and 2,4,6-positions are not obtained
    by the direct chlorination of biphenyl. The proportions of PCBs with
    1-9 chlorine substituents in the Aroclors are shown in Table 1.

        Table 1.  Approximate composition of Aroclors
                                                                                             

                                       Aroclor
                                                                                             
    No. of CI atoms   % of chlorine
    in molecule       weight           1221a     1242b     1248b    1254b    1260b
                                                                                             

    0                     0            12.7
    1                  18.8            47.1        3
    2                  31.8            32.3       13         2
    3                  41.3                       28        18
    4                  48.6                       30        40       11
    5                  54.4                       22        36       49       12
    6                  59.0                        4         4       34       38
    7                  62.8                                           6       41
    8                  66.0                                                    8
    9                  68.8                                                    1
                                                                                             

    a  Willis & Addison (1972).
    b  Panel on Hazardous Trace Substances (1972).

    
    CHEMICAL STRUCTURE

        There have been several investigations to identify individual PCBs
    in commercial products. Sissons & Welti (1971) separated the
    components of the Aroclors by column and gas-liquid chromatography,
    and characterized many of the peaks by high-resolution mass
    spectrometry and nuclear magnetic resonance, and by comparison with 40
    synthesized Webb & McCall (1972) identified the gas-liquid
    chromatographic with those of synthesized compounds by retention times
    and spectrometry (Table 2). The most exhaustive study is that of
    Jensen & Sundström (1974). They recognized that conventional
    gas-liquid chromatography could not separate all the components, so
    they devised preliminary fractionation on a charcoal column, which
    separated the component PCBs according to the number of graphs in the
    2,2',6 or 6' positions in the molecule ( o-chlorines). They compared
    the gas-liquid chromatographic peaks with those of 90 synthesized
    PCBs, and were able to characterize and quantify 60 components of
    Clophens A50 and A60 (Table 3). Tables 2 and 3 show a considerable
    overlap between the components of Aroclor 1254 and Clophen A50.

        Table 2.  Polychlorinated biphenyls in Aroclors 1221-1254 (Webb & McCall, 1972)
                                                                                             

    Retention    Synthetic       Acolor
    timea      chlorobiphenyl                                                                  
                                 1221       1232     1242       1248     1254
                                                                                             

    10         Biphenyl          xb Cc      x        x          x
    13         2                 x C        x        x          x
    16.9       3                 x Dd
    17         4                 x C        x
    17.7
    18.5       2,2'              x C        x        x C        x
    21.3                         x          x        x
    21.6                         x          x        x
    23.5       2,3'              x C        x        x          x
    24         2,4'              x C        x        x C        x
    26         2,6,2'                       x        x D        x
    29         2,5,2'                       x        x C        x
    29.2       2,4,2'                       x        x          x C
    29.5       4,4'              x C        x        x C
    34         2,3,2'                       x        x Ce       x
    38         2,4,3'                       x        x C        x
    38.5       2,4,4'                       x        x C        x
    38.7       2,5,4'                       x        x C        x
    41.5                                             x          x
    42.5       3,4,2'                                x C        x
    43                                               x          x
    44                                               x          x
    44.5                                                        x
    48         2,5,2',5'                             x C        x        x C
    49.5       2,4,2',5'                             x C        x        x C
    50.5       2,4,2',4'                             x D        x
    51.5                                             x          x
    55         2,3,2',5'                             x          x        x C
    57                                               x          x
    59         2,3,6,2',6'                           x          x        x D
    60                                               x          x
    64                                               x          x
    69                                               x          x
    69.5                                                        x        x
    70         2,5,3',4'                             x Ce       x
    70.5       2,3,6,2',5'                                               x C
    71         2,4,3',4'                             x Ce       x
    72                                               x          x
    76         2,3,6,2',3'                                               x C
                                                                                             

    Table 2. (cont'd).
                                                                                             

    Retention    Synthetic       Acolor
    timea      chlorobiphenyl                                                                  
                                 1221       1232     1242       1248     1254
                                                                                             

    82                                                          x
    83         2,3,6,2',5'                                      x        x C
    84                                                          x
    85         2,4,5,2',5'                                      x        x C
    87         2,4,5,2',4'                                      x        x C
    96                                                                   x
    99         2,4,5,2',3'                                      x        x C
    101                                                         x        x
    104                                                         x        x
    107                                                         x        x
    115                                                                  x
    119                                                         x        x
    125        2,4,5,2',3',6'                                            x C
    126        2,4,5,3',4'                                               x C
    135                                                                  x
    147                                                                  x
    148        2,4,5,2',4',5'                                            x C
    149                                                                  x
    152                                                                  x
    165                                                                  x
    178                                                                  x
                                                                                             

    a  Relative to p,p'-DDE at 190°C on 1 00' × 0.02' SCOT SE 30 column.
    b  indicates a GLC peak in this Aroclor at this retention time.
    c  C means that the synthetic compound in the second column was
       confirmed by GLC and IR in this Aroclor. Because of the labour
       involved, most compounds were only confirmed in one Aroclor. For
       example, biphenyl is probably present in 1232-1248 as well as 1221.
    d  Only GLC data available.
    e  Something else also present.

    
    2.2  Purity of Products

        Commercial PCBs are not sold on a composition specification, but
    on their physical properties. Different batches may vary somewhat from
    the compositions shown in Tables 1-3. Impurities known to be present
    in commercial PCBs are chlorinated dibenzofurans and chlorinated
    naphthalenes (Vos et al., 1970; Bowes et al., 1975). Bowes et al.
    (1975) found chlorinated dibenzofurans at 0.8-2.0 mg/kg in samples of
    the Aroclor 1248-1260 series, but none in Aroclor 1016, and at levels
    of 8.4 mg/kg in Clophen A60 and 13.6 mg/kg in Phenoclor DP-6. Roach &
    Pomerantz (1974) found chlorinated dibenzofurans levels of 1 mg/kg and
    Nagayama et al. (1976) found 18 mg/kg in different batches of
    Kanechlor 400, but no chlorinated dibenzodioxins.

    2.3  Determination of PCB Residues

        Reviews have been published on methods used for the determination
    of organochlorine compounds including PCBs in environmental samples
    (Holden, 1973a, Panel on Hazardous Trace Substances, 1972). No two
    laboratories have identical methods, although all have features in
    common. The techniques appear to be those previously developed for the
    determination of organochlorine pesticides with appropriate
    modifications for the presence of PCBs, and the studies on PCBs
    sometimes form part of a wider programme for monitoring persistent
    organochlorine compounds in the environment. The major difficulties in
    the determination of PCBs are to separate them from interfering
    organochlorine pesticides, and to derive a single quantitative figure
    from a variable mixture of components.

    2.3.1  Extraction of sample

    Air

        Particulate fallout from air has been trapped on 200 µm nylon net
    coated with silicone oil, and the PCBs then extracted with hexane
    (Södergren, 1972a). Separate determinations of particulate and vapour
    phase PCBs in air have been made by the passage of a large volume of
    air through a filter which was followed by an impinger containing
    hexane (Hasegawa et al., 1972b), or a polyurethane plug (Bidleman &
    Olney, 1974) or ceramic saddles coated with OV 17 silicone (Harvey &
    Steinbauer, 1974) to absorb the vapour.



        Table 3.  Percentages of polychlorinated biphenyls in Clophen A50 and A60 and in human (Jensen & Sundström, 1974)
                                                                                                                                

                                                                                    Percentage in
                                                             Relative                                                           
    Compound                             No. of           retention time         Clophen
                                       orthochlorines                                               Human
    No.        Structurea                                 Apiezon L  SF 96       A50       A60        tissue
                                                                                                                                

    1          2,5-2'5'                     2                0.25       0.30        5.0
    2          2,4-2'5'                     2                0.26       0.30        1.4
    3          2,3-2'5'                     2                0.27       0.33        1.9                  1.1
    4          (2,-2',3',4')                2                0.30       0.43        1.2                  0.66
    5          (4-2',3'6')                  2                0.32       0.43        2.1                  0.56
    6          2,5-2',3',6'                 3                0.35       0.43        4.4b      2.9        1.2
    7          2,3-2',3',6'                 3                0.38       0.49        2.5b      0.28       0.48
    8          3,4-2',5'                    1                0.41       0.42        3.9                  1.5
    9                                       1 or 2           0.42                   2.2c                 2.0c
    10         2,5-2',3',5'                 2                0.45       0.49        2.2       1.1        1.2
    11         2,3,6-2',3',6'               4                0.47       0.61        0.50      1.0d
    12         2,5-2',4',5'                 2                0.48       0.50        7.0b      5.6        4.2
    13         2,4-2',4',5'                 2                0.51       0.51        1.8b      --e        1.9
    14         2,3-2',4',5'                 2                0.53       0.57        1.4b
    15         2,5-2',3',4'                 2                0.53       0.58        5.4       1.4        2.3
    16         2,3-2',3',4'                 2                0.55       0.66        1.0
    17         3,4-2',3',6'                 2                0.56       0.62        7.6b      2.9        4.7
    18         2,3,5-2',3',6'               3                0.60       0.70        1.2       4.2        1.0
    19         2,4,5-2',3',6'               3                0.64       0.73        2.0b      6.5d       0.13
    20         2,5-2',3',5',6'              3                0.65       0.68        1.3       3.3        0.43
    21         2,3-2',3',5',6'              3                0.70       0.74        0.5                  0.05
    22         2,3,4-2',3',6'               3                0.72       0.83        1.8       3.2        0.15
    23                                      1 or 2           0.76                   0.6c
                                                                                                                                

    Table 3.  (cont'd).
                                                                                                                                

                                                                                    Percentage in
                                                          Relative                                                           
    Compound                             No. of           retention time         Clophen
                                       orthochlorines                                               Human
    No.        Structurea                                 Apiezon L   SF 96       A50       A60        tissue
                                                                                                                                

    24         2,3,6-2',3',5',6'            4                0.77       0.91        0.09      0.96
    25         3,4-2',4',5'                 1                0.79       0.74        5.0b      1.6        5.4
    26         2,3,6-2',3'4',6'             4                0.84       0.95        0.05      0.37
    27         2,3,52',4',5'                2                0.85       0.86        0.90      2.9        2.7
    28         3,4-2',3',4'                 1                0.87       0.86        3.6                  1.9
    29         2,4,5-2',4',5'               2                0.90       0.86        4.2b      12.9d      21.5
    30         2,3,4-2',3',5'               2                0.94       0.97 )                1.5        --e
    31                                      1 or 2           0.95       0.93 )      1.1       --e
    32         2,3,4-2',4',5'               2                1.00       1.00        5.1       11.3d      14.0
    33         2,3,5-2',3',5',6'            3                1.01       1.06        0.04      0.49       0.90
    34                                      4                1.02                   0.05c
    35                                      1 or 2           1.04       1.10        1.1c      2.0c       1.5'
    36         2,4,5-2',3',5',6'            3                1.05       1.13        0.39      3.3        3.5
    37         2,3,4-2',3',4'               2                1.11       1.16        1.3       2.0        0.81
    38         2,3,6-2',3',4',5'            3                1.13       1.29        0.33      3.7d       --e
    39         2,4,5-2',3',4',6'            3                1.14       1.16        0.17      1.8        2.5
    40         2,3,4-2',3',5',6'            3                1.19       1.32        0.27      2.1        1.3
    41         2,3,5,6-2',3',5',6'          4                1.23       1.34        0.005     0.07
    42         2,3,4-2',3',4',6'            3                1.28       1.40        0.13      1.3        0.57
    43         2,3,4,6-2',3',5',6'          4                1.34       1.44        0.007     0.09
    44         2,4,5-3',4',5'               1                1.41       1.19        0.47      1.0        0.49
    45         2,3,6-2',3',4',5',6'         4                1.45       1.59        0.008     0.09
    46         2,3,4,5-2',3',4',6'          4                1.46       1.51        --e       0.03
    47         3,4-2',3',4',5'              1                1.55       1.37        0.81      1.5        2.0
    48         2,3,5-2',3',4',5'            2                1.59       1.49        0.23      0.90       1.2
    49         2,4,5-2',3',4',5'            2                1.71       1.56        0.98      7.6d       7.7
                                                                                                                                

    Table 3.  (cont'd).
                                                                                                                                

                                                                                    Percentage in
                                                          Relative                                                           
    Compound                             No. of           retention time         Clophen
                                       orthochlorines                                               Human
    No.        Structurea                                 Apiezon L   SF 96       A50       A60        tissue
                                                                                                                                

    50         2,3,4-2',3',4',5'            2                1.88       1.82        0.72      4.1d       5.9
    51         2,3,4,5-2',3',5',6'          3                1.94       1.99        0.08      0.74       0.77
    52                                      1 or 2           2.02       1.96        0.23c     1.0c       --e
    53         2,3,4,5-2',3',4',6'          3                2.07       2.05        0.06      0.44       0.94
    54         2,4,5-2',3',4',5',6'         3                2.15       2.04        0.01      0.28       0.46
    55         (2,3,5,6-2',3',4',5',6')     4                2.23                                        --e
    56         2,3,4-2',3',4',5',6'         3                2.40       2.20        0.01      0.17       0.31
    57         2,3,4,6-2',3',4',5',6'       4                2.45       2.56                  --e        --e
    58         3,4,5-2',3',4',5'            1                2.74       2.41        --e       --e
    59         2,3,4,5-2',3',4',6'          2                3.18       2.81        0.35      0.67       1.7
    60         2,3,4,5,6-2',3',4',5',6'4    4                4.12                                        0.62

                                                                        Total       86.7%     99.4%      100.0%
                                            p,p'-DDE         0.52       0.58
                                            p,p'-DDT         0.71       0.73
                                            p,p'-DDT         0.90       0.97
                                                                                                                                

    a  Tentative structures are given in brackets.
    b  Component found present in Aroclor 1254 by Webb & McCall (1972).
    c  Figures calculated using responses of chlorobiphenyls with similar retention times in the same fraction
       from the charcoal column.
    d  Component found present in Phenoclor DP6 by Tas & Vos (1971).
    e  Present in trace amounts only.

    


    Water

        PCBs have been extracted from water by passing a sample through a
    filter of undecane and Carbowax 400 monostearate supported on
    Chromosorb W (Ahling & Jensen, 1970) or a porous plug of polyurethane
    coated with a suitable gas-liquid chromatographic stationary phase
    (Uthe et al., 1972), or Amberlite XAD-2 resin (Harvey, et al., 1973)
    followed by elution of the PCBs with a solvent. Ahnoff & Josefsson
    (1975) have described a liquid-liquid extraction into cyclohexane.

    Biological samples

        Most analysts have used standard methods developed for
    organochlorine pesticides, in which the PCBs are extracted together
    with the fat; the sample is ground with anhydrous sodium sulphate and
    extracted with petroleum ether or hexane. Porter et al. (1970) studied
    the optimal conditions for this procedure. A dehydrating solvent may
    be included to facilitate the breakdown of cell structures; ethanol
    (Norén & Westöö, (1968) and acetone (Jensen et al., 1973) have been
    used. Rote & Murp (1971) digested the sample with a mixture of acetic
    and perchloric acid prior to hexane extraction.

    2.3.2  Clean-up

        Methods for the removal of fat from the extract include solve
    partitioning between hexane and acetonitrile or dimethylformamide,
    treatment with strong sulfuric acid or ethanolic potassium hydroxide
    Gel permeation has also been used (Stalling et al., 1972), and Holden
    Marsden (1969) removed fat on dry, partially deactivated alumina
    column. Certain pesticides such as dieldrin are destroyed by the
    sulfuric at treatment, so this method cannot be used if such
    pesticides are to determined together with PCBs (Jensen et al., 1973).

        PCBs may be separated from organochlorine pesticides by column
    chromatography on Florisil (Mulhern et al., 1971), silica gel (Holden
    Marsden 1969; Armour & Burke, 1970; Collins et al., 1972) or on
    charcoal (Berg et al., 1972; Jensen & Sundström, 1974). Several
    laboratories have reported difficulties in repeating results obtained
    by other investigators; the ease of separation appears to depend upon
    the characteristics of absorbent, of the eluting solvent, and of the
    sample extract, though the appears to be no difficulty in separating
    all interfering substances except DDE, a metabolite of DDT. Thin-layer
    chromatography has been us for separation by Norén & Westöö (1968),
    Bagley et al. (1970), and Rein et al. (1973).

        In many environmental samples, DDE is present in large excess over
    the PCBs, and must be removed before the quantitative determination
    PCBs. Oxidation procedures have been used to convert DDE
    dichlorobenzophenone; recommended oxidants are potassium dichromate
    and sulfuric acid (Westöö & Norén, 1970b) and chromium (II) oxide a
    acetic acid (Mulhern et al., 1971). Jensen & Sundström (1974), who we
    interested in determining DDT/PCB ratios in environmental sample
    preferred sodium dichromate in acetic acid with a trace of sulfuric
    acid. They claim that this does not destroy DDT and its metabolite DDD
    which may be present in extracts after clean-up with strong sulfuric
    acid and that using this mixture makes possible the quantitative
    determination of the dichlorobenzophenone from the oxidation of DDE.

        Conversion of DDT to DDE may be achieved by treatment with
    ethanolic potassium hydroxide, which also removes interference from
    elemental sulfur (Ahling & Jensen, 1970). Sulfur may also be removed
    activated Raney nickel (Ahnoff & Josefsson, 1975) or by metallic
    mercury.

        Södergren (1973b) has scaled down the clean-up procedure for small
    samples, using microlitre volumes.

    2.3.3  Chromatographic separation of PCBs

    Gas-liquid chromatography

        Most analysts use gas-liquid chromatography with an electron-
    capture detector for the separation of PCBs from the extract after
    clean-up. Stationary phases commonly used are silicones or their
    derivatives, for example, DC 200, SF 96, OV 1, and QF 1, or Apiezon L.
    Jensen & Sundström (1974) state that with a mixture of SF 96 and QF 1,
    14 peaks can be obtained from Clophen A50, but that Apiezon L gives
    much better resolution. They obtained better peak separation by prior
    fractionation on a charcoal column, which separated the PCBs according
    to the number of  o-chlorine substituents; they regard such
    refinements as unnecessary in PCB residue analysis, although they may
    be of value in the study of the selective, environmental degradation
    of PCBs. Column temperatures used ranged between 170°C and 230°C.
    Glass capillary columns gave good separation of PCBs from DDT and its
    metabolites (Schulte & Acker, 1974).

    Thin-layer chromatography

        This has been used in the clean-up stage, but it may also provide
    semi-quantitative results by visualization of the spots followed by
    densitometry, or by comparison with spots produced by known amounts of
    PCBs. Mulhern et al. (1971) separated PCBs on a plate coated with
    alumina containing silver nitrate, and the spots were developed by

    exposure to ultraviolet light; the detection limit was in the region
    of 1 µg. Collins et al. (1972) devised a similar method in which the
    PCBs remained together in a single spot, and they claimed a limit of
    detection of about 50 ng. Reversed-phase chromatography on plates
    coated with kieselguhr treated with liquid paraffin has been used to
    separate Phenochlor DP6 into several spots with a detection limit of a
    few micrograms (de Vos & Peet, 1971).

    2.3.4  Quantification of PCB content

        The response of the electron capture detector is not equal for all
    PCB components, being much affected by the degree of chlorination
    (Zitko et al., 1971). This does not lead to difficulties when the
    sample under investigation has been directly contaminated by a
    commercial PCB mixture, as that mixture can be used as a standard.
    Difficulties are encountered when the PCBs in the sample have
    undergone selective environmental degradation (see sections 3 and 5).
    Several investigators have noted that the pattern of peaks from such
    samples resembles fairly closely that of one or other of the higher
    chlorinated PCB mixtures such as Aroclor 1254, and they have compared
    the total area of the peaks with that of the nearest commercial
    product in order to determine the amount of PCBs in the sample (Armour
    & Burke, 1970). Collins et al. (1972 observed that, under their
    conditions, the area of peaks usually encountered in extracts of
    tissue samples was closely similar to that of an equivalent amount of
    DDE, thus DDE could be used for calibration. In order to overcome the
    uncertainties of these procedures, Rote & Murphy (1971) divided the
    peaks into groups according to the number of chlorine atoms in the
    molecule, as determined from mass spectrographic data, and calculated
    the PCB content of each group from the theoretical response of the
    detector to chlorine content. Jensen et al. (1973) selected a
    commercial in PCB that included all the peaks from the extract; they
    determined the PCB content of each peak by combined mass spectrometry
    and coulometry, and determined the total PCBs in the sample by
    comparing the height of each peak obtained with the extract with those
    obtained with the reference sample. Simpler methods have been used by
    Koeman et al. (1969), who compared the height of a single peak
    obtained with the extract with that of a peak with the same retention
    time obtained with a e commercial PCB mixture; others have averaged
    out more than one peak s for this calculation (Reynolds, 1971; Reinke
    et al., 1973). Rote & Murphy (1971) have calculated that such
    procedures may more than double the values obtained by a more accurate
    method.

        A different technique has been recommended by Berg et al. (1972);
    the PGBs are chlorinated with antimony pentachloride to decachloro-
    biphenyl, which can then be measured as a single peak.

    2.3.5  Accuracy of PCB determinations

        A group of eight analysts engaged in an investigation of pollution
    in the North Sea undertook a collaborative study to determine the PCB
    content of a sample of fish oil, using the methods currently employed
    in their laboratories (International Council for the Exploration of
    the Sea, 1974). The PCB values obtained ranged from 1.0 to 3.9 mg/kg
    with a mean of 1.97 mg/kg and a standard deviation of 0.93 mg/kg.
    Better agreement was obtained with the same fish oil fortified with
    PCBs at a concentration of 10 mg/kg; the mean of the results for the
    fortified sample was 10.0 mg/kg with a standard deviation of
    1.1 mg/kg.

        A probable source of error is incomplete initial extraction of
    PCBs from the sample (Holden & Marsden, 1969). Another source of
    variation between laboratories lies in the method used to quantify
    gas-liquid chromatographic peaks (section 2.3.4); van Hove Holdrinet
    (1975) considered this to be the major source of error.

        It is evident that caution should be exercised in accepting the
    analytical results from a laboratory, particularly for samples with a
    low PCB content, until the competence of that laboratory has been
    established by an inter-laboratory collaborative study.

    2.3.6  Confirmation of identity

        Since Jensen first identified as PCBs those hitherto unknown
    substances that interfered in the gas-liquid chromatographic
    determination of organochlorine pesticides using mass spectrographic
    data, other investigators have confirmed the presence of PCBs in
    environmental samples by combining gas-liquid chromatography with mass
    spectrometry (Bagley et al., 1970) and with coulometry to measure the
    chlorine content. The conversion of PCBs to bicyclohexyl and
    decachlorobiphenyl is further confirmation (Berg et al., 1972). The
    widespread distribution of PCBs is now well established, and, as
    adequate methods are available to remove interference from
    organochlorine pesticides, there is no evidence of the presence of
    other interfering substances in the types of sample that have so far
    been analysed, down to a limit of detection of around 0.01 mg/kg. This
    does not necessarily apply to other types of sample, particularly when
    very low levels are being sought; Ahnoff & Josefsson (1973, 1975)
    reported a number of unknown interfering substances, when measuring
    PCBs in water at levels below 1 ng/litre, one of which was
    subsequently identified as elemental sulfur. They recommend
    confirmation by mass fragmentography for such samples.

    2.4  Determination of PCTs

        A few methods have been published for the determination of PCTs;
    extraction and clean-up procedures are similar to those used for PCBs,
    but the gas-liquid chromatographic details are different because of
    the lower volatility of the PCTs. Zitko et al. (1972b) used 3% OV 210
    as the stationary phase with a column temperature of 200°C. Thomas &
    Reynolds (1973) also used OV 210 with a column temperature of 250°C
    and another system with 3% Dexsil as stationary phase at 300°C with a
    63Ni electron capture detector; this was also used by Addison et al.
    (1972). Sosa-Lucero et al. (1973) used OV 210 and SE 30 at 255°C and
    Freudental & Greve (1973) used OV 17 with a temperature programmed
    from 200°C to 285°C. Thomas & Reynolds (1973) confirmed the identity
    by chlorination to tetradecachloroterphenyl with antimony
    pentachloride.

        A thin-layer chromatographic technique has also been described
    with limit of detection of about 1 µg (Addison et al., 1972).

    3.  SOURCES OF ENVIRONMENTAL POLLUTION

    3.1  Production and Uses of PCBs

        Details of the production and uses of PCBs in the USA have been
    released, and have been summarized by Nisbet & Sarofim (1972). Annual
    production has increased steadily since 1930 and reached a maximum in 
    1970 of 33 000 tonnes. During this peak year, 65% of the production
    was of the 42% chlorinated type, 25% was less chlorinated, and the
    remainder more chlorinated. After 1970, production sharply decreased
    owing to voluntary limitation of sales by the Monsanto Company, the
    sole manufacturer in the USA. According to information collected by
    the Organization for Economic Co-operation and Development (OECD), the
    1970, production in the USA was 18 000 tonnes and the total in OECD
    countries in that year was 48 000 tonnes. It has been estimated that
    the cumulative total production of PCBs in North America up to 1971
    was 0.5 million tonnes, and in the whole world probably double this
    figure.

        The commercial applications of PCBs have been reviewed in an OECD
    report (Organization for Economic Co-operation and Development, 1973)
    From an environmental viewpoint these can be divided into three
    categories:

     Controllable closed systems. PCBs used as dielectrics in
    transformers and large capacitors have a life equal to that of the
    equipment, and with proper design leakage does not occur. When the
    equipment is scrapper the quantity of dielectric is sufficiently large
    to justify regeneration.

     Uncontrollable closed systems. PCBs are used in heat transfer and
    hydraulic systems which, although technically closed, permit leakage.
    The need for frequent replacement of small quantities makes recovery
    impracticable, PCBs are very widely dispersed in small capacitors, and
    there are great difficulties in collecting these items for disposal.

     Dissipative uses. PCBs have been used in the formulation of
    lubricating and cutting oils, in pesticides, and as plasticizers in
    paints, copying paper, adhesives, sealants, and plastics. In these
    applications, the PCBs are in direct contact with the environment, and
    there is no way of recovering them when the product is scrapped.

        The uses of PCBs in the USA in 1970 have been analysed by Nisbet &
    Sarofim (1972). Of the total 33 000 tonnes, 56% was used as a
    dielectric, with 36% in capacitors and 20% in transformers. Various
    plasticizer outlets accounted for 30%, hydraulic fluids and lubricants
    12%, and heat transfer liquids 1.5%. Following the restriction of
    sales for non-dissipative uses, the percentage of PCBs sold as
    dielectrics rose to 77% in 1971 and the proportion of highly

    chlorinated products was considerably reduced, Aroclor 1016 replacing
    Aroclor 1242. In Japan, 44 800 tonnes of PCBs were used from 1962 to
    1971, and of this 65.4% was used in the electrical industry, 11.3% in
    heat exchangers, 17.9% in pressure-sensitive duplicating paper, and
    5.4% for other dissipative uses (Ishi, 1972). In Sweden, most of the
    600 tonnes imported in 1969 was used in the electrical industry and a
    large part of the remainder in paints (Jensen, unpublished report
    1972).

        According to the OECD report, transformers and capacitors provided
    the major outlets for PCBs in most OECD countries in 1971. In 1972,
    several countries restricted sales; in Sweden the importation and use
    of PCBs were restricted by law; in the United Kingdom, as in the USA,
    sales were voluntarily restricted to the lower chlorinated PCBs for
    use as dielectrics in enclosed systems; in the Federal Republic of
    Germany, use in hydraulic and heat transfer fluids was also permitted.
    In Japan the production and use of PCBs were banned in 1972.
    Limitations on sales were subsequently introduced in other countries.

        No information is available on the scale of production and the
    uses of PCTs.

    3.2  Entry of PCBs into the Environment

        Surveys of the sources of environmental pollution with PCBs were
    made before production and use were limited, and the information
    available may not now apply in North America and elsewhere. Table 4
    gives an estimate of the fate of the PCBs produced in 1970 in the USA
    (Nisbet & Sarofim, 1972). Only 20% of the annual production can be
    regarded as a net increase in current usage, and the remainder is
    balanced by a loss to the environment. More than one-half of this
    entered dumps and landfills and it has been calculated that 0.3
    million tonnes of PCBs have accumulated in such locations in North
    America since 1930. Much of this was originally enclosed in containers
    such as capacitors or was in plasticized resins and will not be
    released until the containing medium decays. The diffusion of PCBs
    from landfills is likely to be slow on account of their low volatility
    and low water solubility; Carnes et al. (1973) found little leaching
    from one site they tested.

        Pollution of the environment has occurred mainly from the first
    three routes mentioned in Table 4. In addition, there are other
    routes, which although involving relatively small amounts,
    nevertheless have an influence on the entry of PCBs into food chains.
    PCBs have been used in the USA in amounts of about 10 tonnes/year in
    pesticide formulations (Panel on Hazardous Trace Substances, 1972),
    and the unauthorized use of scrap transformer fluid for this purpose
    has led to local contamination of milk supplies.

        Table 4.  Entry of PCBs into the environmenta
                                                                                             

                                                      Percentage of
                                                      annual               PCB type
    Route                                             production           (% chlorination)
                                                                                             

    Vaporization from plasticizers                      4.5                48-60
    Vaporization during incineration                    1                  42
    Leaks and disposal of industrial fluids            13                  42-60
    Destruction by incineration                         9                  mainly 42
    Disposal in dumps and landfills                    52.5                42-60
    Net increase in current usage                      20                  42-54
                                                                                             

    a  From Nisbet & Sarafim, 1972.
    
        Pressure sensitive duplicating paper containing PCBs has found its
    way into waste paper supplies and has been recycled into paper and
    board used as food packaging materials; paints for coating the bottom
    of ships contained 3-5% of PCBs -- about 3% of the annual quantity
    imported into Sweden has been used for this purpose -- and this has
    been a source of plankton contamination (Jensen et al., 1972a).

    3.2.1   Release of PCBs into the atmosphere

        There appears to be little widespread atmospheric contamination
    during the manufacture and processing of PCBs, but this can occur
    during their subsequent use and disposal. Although PCBs have a low
    volatility, there may be an appreciable loss to the atmosphere during
    the lifetime of a PCB-plasticized resin, particularly of the lower
    chlorinated products. Further pollution may occur during the
    incineration of industrial and municipal waste. Most municipal
    incinerators are not very effective in destroying PCBs; efficient
    incinerators can be designed for this purpose (Jensen & Wickberg,
    unpublished report 1971; Jensen, unpublished report 1972), although
    the higher chlorinated PCBs are more resistant to pyrolysis. Secondary
    sources of atmospheric pollution are volatilization from soil, and
    from the drying of sewage sludge. Laveskog (1973) found that the PCB
    emissions from municipal incineration and from the drying of sewage
    sludge each amounted to about 1 kg/year per million inhabitants, a
    small amount compared with the 2 tonnes deposited yearly from aerial
    fallout in the south of Sweden.

    3.2.2  Leakage and disposal of PCBs in industry

        The major source of environmental pollution with PCBs, which
    eventually affects food chains, is the leakage and the disposal of
    industrial fluids. There have been serious cases of poisoning in man
    (Kuratsune et al., 1972) and in animals (Panel on Hazardous Trace
    Substances, 1972) due to leakage from a heat exchanger. Leakages, or
    the unintentional or deliberate discharge of waste, have contaminated
    seas, lakes, waterways, and sewers (Duke et al., 1970; Schmidt et al.,
    1971; Veith, 1972).

        Analysis of solids from factory wastes in Japan have revealed a
    wide variation in PCB content. In most, less than 1 mg/kg was
    detected, but in: some the contamination was very heavy, the highest
    level recorded being 8.26% from a factory manufacturing electrical
    equipment (Japanese Environment & Safety Bureau, unpublished report
    1972).

    4.  ENVIRONMENTAL TRANSPORT AND TRANSFORMATION

    4.1  Environmental Transport

        Nisbet & Sarofim (1972) emphasize that the available data are
    insufficient to determine anything but a very crude model of the
    transfer of PCBs into the environment. Guesses can be made by
    referring to the distribution of DDT, which resembles the PCBs to some
    extent in its physical and chemical properties and about which more is
    known. Much of the following discussion arises from their analysis of
    the situation in the North American continent.

    4.1.1  Air transport

        By analogy with DDT, it might be expected that PCBs entering the
    atmosphere in the vapour phase would be adsorbed rapidly on to
    particles, which would be deposited or washed out in rain at a rate
    depending on their particle size, the average residence time in the
    atmosphere being 2-3 days. This has been confirmed by Södergren
    (1972a), who measured the deposition in southern Sweden of PCBs that
    had originated from municipal incinerators or had been carried over
    from Denmark by the prevailing winds, and showed that the amount
    deposited in central Sweden was much less. Cames (1973) and Laveskog,
    (unpublished report 1973) found mainly particulate PCBs in the
    emission from incinerators. Harvey & Steinhauer (1974), however,
    considered that the results of their analyses of air in north Atlantic
    regions indicated that most of the PCBs carried in the air were in the
    vapour phase.

    4.1.2  Transport in soil

        The PCBs in soil are derived mainly from particulate deposition,
    estimated at 1000-2000 tonnes annually in North America, most of which
    is in urban areas. Small amounts have originated from the use of
    sewage sludge as a fertilizer, from the leaching of landfalls, and
    from the se of PCBs in pesticide formulations. Tucker et al. (1975)
    found that under experimental conditions, the higher chlorinated PCBs
    were not leached from soils by percolating water, and those with a
    lower level of chlorination were removed only slowly, particularly
    from soils of high lay content. Losses do occur by volatilization and
    by biotransformation; by analogy with DDT and its metabolites the
    half-time in soil has been estimated at 5 years. Haque et al. (1974)
    showed that the rate of evaporation decreased with the clay content of
    the soil and he degree of chlorination of the biphenyl, and increased
    with temperature. Biotransformation has also been shown to play a part
    in the disappearance of the lower chlorinated compounds from soil
    (Iwata et al., 1973).

        The total amount of PCBs distributed over North America, apart
    from that in dumps and landfills, has been estimated at 20 000 tonnes,
    of which one quarter has subsequently been transported via the air to
    the seas. (Nisbet & Sarofim, 1972).

    4.1.3  Transport in water

        The entry of PCBs into water occurs mainly at the points of
    discharge of industrial and urban wastes into rivers, lakes, and
    coastal waters. Sewage treatment appears to remove particulate PCBs
    from water, but not PCBs in solution; these are concentrated in the
    sludge (Ahling & 1970), which may be dumped into rivers and coastal
    waters. Holden (1970b) found a mean PCB content of 3 mg/kg in liquid
    sludges from Glasgow, and calculated that PCBs at the rate of
    1 tonne/year were released into the Clyde and Thames estuaries; a
    similar output was calculated from water treatment plants off the
    California coast (Schmidt et al., 1971). Other localized sources of
    pollution are leakages or waste disposal from ships. PCBs in water are
    attached mainly to particulate matter (Södergren, 1973a) and
    eventually fall to the bottom sediment at a rate that depends on the
    particle size. PCBs may be leached from the sediment and may reach
    coastal waters, but Nimmo et al. (1971) noted little change in the PCB
    content of a sediment at a point downstream from a source of
    contamination over a period of 9 months. The process may be
    accelerated by the dumping of dredging spoil.

    4.1.4  Transport through biota

        According to the approximate calculations of Nisbet & Sarofim
    (1972), less than 1000 tonnes of PCBs are located in living organisms
    throughout the world, so that biological transport and degradation
    play little part in the fate of PCBs in the environment, though these
    factors have great ecotoxicological significance.

    4.2  Transformation in the Environment

    4.2.1  Abiotic transformation

        The fate of the various PCBs in commercial mixtures depends on
    their physical and chemical properties. Some fractionation occurs
    during the volatilization of PCBs, because of a decrease in vapour
    pressure with increasing chlorination. The PCBs are chemically very
    stable, and are not likely to be degraded at a significant rate by
    hydrolytic or similar reactions under environmental conditions. They
    are, however, fairly easily degraded by photolysis under laboratory
    conditions; Safe & Hutzinger (1971), and Hutzinger et al. (1972b) have

    shown that PCBs are dechlorinated in hexane solution at a rate that
    increases with increasing chlorination. PCBs in aqueous-dioxane
    suspensions and in thin films give hydroxy and carboxylic acid
    derivatives on irradiation. The lower chlorinated biphenyls, at a
    vapour concentration in air of 1.5 mg/m3 are readily destroyed by
    photolysis under laboratory conditions. There is no direct evidence of
    the extent of PCB breakdown in the atmosphere under  environmental
    conditions, and nothing is known of the persistence and toxicity of
    any transformation products.

    4.2.2  Biotransformation

        The biotransformation of PCBs is discussed in section 6.6. Owing
    to small proportion of the total environmental PCBs contained in
    living biotransformation does not significantly influence the overall
    environmental concentrations of PCBs, though it has a marked influence
    on PCBs passing through food chains.


    4.2.3  Metabolism in limited ecosystems

        The presence of PCBs in sewage sludge suggests that they are not
    all readily transformed by microorganisms. Choi et al. (1974) found no
    evidence of biotransformation of Aroclor 1254 added to water entering
    an aerated biological water treatment system, although much of it was
    removed with the sludge; there appeared to be no interference by the
    PCBs in the performance of the system. Vodden (1973) quotes
    investigations showing that PCBs with four or fewer chlorine atoms are
    readily broken down by microorganisms, but that this can be inhibited
    by the presence of higher chlorinated PCBs. Mono- and dichlorobiphenyl
    can be transformed by  Achromobacter isolated from sewage effluent
    (Ahmed & Focht, 1973), and a culture of lake bacteria can degrade some
    of the lower chlorinated components of Aroclor 1242 to chlorine-free
    derivatives (Kaiser & Wong, 1974).

        Södergren (1972b) investigated the transport of Clophen A50 added
    to a model aquatic ecosystem; this was rapidly taken up by an alga
     (Chlorella) and no change was observed in the proportion of the
    component PCBs in the first consumer fish; however, there was a
    relative loss of the lower chlorinated PCBs in the perch, the second
    consumer. No progressive loss of the lower chlorinated PCBs was
    observed in sediment, plankton, invertebrates, and fish inhabiting a
    Swedish lake (Södergren, 1973a). Evidence on the metabolism of PCBs by
    fish is conflicting, but it seems probable that most fish,
    particularly those in the lower trophic stages of food chains, cannot
    readily degrade the low chlorinated PCBs.

    4.3  Biological Accumulation

        Although PCB concentrations in living organisms clearly indicate a
    progressive accumulation in food chains, the factors discussed in the
    previous sections make it impossible to give any reliable figure for
    bioaccumulation at each trophic stage. There is also doubt about which
    tissue level should be used in the calculation, that of the whole
    body, the fat, or the liver.

        There is good evidence that all aquatic organisms studied in
    aquaria can absorb PCBs directly from water. The accumulation varies
    with the duration of exposure and the concentration in the ambient
    water. A diatom exposed to Aroclor 1242 showed an accumulation factor
    of 1100 (Keil et al., 1971); with Aroclor 1254 the following values
    have been obtained: pink shrimp, 6600; blue crab, 4600; oyster, 8100;
    pinfish, 980 (Duke et al., 1970); spot, 37 000 (Hansen et al., 1971);
    bluegills, up to 71 400 (Stalling & Mayer, 1972). The accumulation
    factor in scud exposed to Aroclor 1254 reached a maximum of 24 000
    within 4 days and thereafter remained fairly constant (Sanders &
    Chandler, 1972). Similar results were obtained with other
    invertebrates, although with crayfish the uptake was slower and the
    accumulation was still increasing after 21 days. However, it is
    probable that most of the PCBs entering aquatic systems are retained
    by particulate matter, and the above accumulation factors are not
    necessarily applicable to natural ecosystems. Nimmo et al. (1971)
    demonstrated that the fiddler crab could ingest PCB contained in the
    bottom sediment. When exposed to Aroclor 1254 in water, ciliated
    protozoa, stated to be the major benthic input to aquatic food chains,
    had an accumulation factor of 60 (Cooley et al., 1972).

    5.  ENVIRONMENTAL LEVELS AND EXPOSURES

    5.1  Air

        Mean concentrations in air, in several locations in Sweden, ranged
    from the detection limit of 0.8 ng/m3-3.9 ng/m3. The highest figure
    recorded was 12.5 ng/m3 (Ekstedt & Odén, 1974). In the USA, PCB
    concentrations in air ranged from 5 ng/m3 near the north-east coast
    to 0.05 ng/m3 at a distance of 2000 m out over the Atlantic Ocean
    (Harvey & Steinhauer, 1974). Results from the United States
    Environmental Protection Agency indicate a range between 1 and
    50 ng/m3 (Panel on Hazardous Trace Substances, 1972), and similar
    results have been reported from Japan (Tatsukawa & Watanabe, 1972).

    5.2  Soil and Sediments

        In Sweden, PCBs have been found in natural soil at a concentration
    of 15 µg/kg by Odén & Berggren (1973). The same authors also found
    0.006-1.4 mg/kg in sediments from areas in the Baltic Sea with
    different degrees of pollution. Nimmo et al. (1971) found PCB levels
    of 1.461 mg/kg in sediment from an estuary at a point near the site of
    an accidental release of PCBs from a factory, and levels of 0.6 mg/kg
    at a point 16 km downstream. Soil samples from the bank 6.5 km
    downstream from the source contained 1.4-1.7 mg/kg. Less than 1 mg/kg
    has been found in Japanese agricultural soil, but as much as 510 mg/kg
    in soil near a factory making electrical components (Fukada, et al.,
    1973).

    5.3  Water

        In heavily contaminated waters, PCB concentrations may be several
    times greater than their solubility, owing to adsorption on suspended
    particles (Duke et al., 1970). Water in a Swedish river contained
    0.5 ng/litre as it entered a water treatment plant, and 0.33 ng/litre
    in the tap water produced (Ahling & Jensen, 1970). Values of
    0.1-0.3 ng/litre have been measured in other Swedish rivers (Ahnoff &
    Josefsson, 1974). Södergren (1973a) found a seasonal variation in the
    PCB level in a Swedish lake, with a maximum of 2 ng/litre, the
    pollution being attributed to aerial fallout. Concentrations of
    10-100 ng/litre have been measured in tap water at Kyoto in Japan
    (Panel on Hazardous Trace Substances, 1972)œ In a polluted coastal
    area of Lake Michigan in 1970, PCB concentrations of from 450 to less
    than 100 ng/litre were measured in 1970, but there was a marked
    decrease in 1971, possibly due to the limitation on sales of PCBs
    (Panel on Hazardous Trace Substances, 1972). From the scanty
    information on PCBs, reinforced by analogy with the more extensive
    information on DDT, it has been estimated that nonpolluted fresh
    waters should contain not more than 0.5 ng/litre up to 5 ng/litre for
    the Great Lakes of North America, 50 ng/litre for moderately polluted
    rivers and estuaries, and 500 ng/litre for highly polluted rivers.

    5.4  Living Organisms

        There is now considerable information from Canada, Japan, Sweden
    the United Kingdom, and the USA, on the accumulation of PCB's
    biological material. Analytical measurements on different organisms,
    and on the same organism from different localities, vary widely and it
    is necessary to consider the factors that lead to this variation.
    Difference in analytical techniques may contribute to this (see
    section 2.3.5) but important influences are exerted by the extent of
    local pollution, amount of fat in the organism studied, and its
    trophic stage in food chains.

    5.4.1  The influence of local pollution

        Most of the fish eaten by man is taken from waters with little
    pollution. In a collaborative study by seven national (International
    Council for the Exploration of the Sea, 1974), the PCB content of
    muscle tissue of fish taken from the North Sea was measured. A mean of
    0.01 mg/kg was found in cod, herring contained up to 0.48 mg/kg with
    most samples in the range of 0.1-0.2 mg/kg, and plaice contained
    0.1 mg/kg or less. Similar values were reported by Zitko (1971) for
    fish taken from the North Atlantic.

        There are many examples of different PCB levels in similar species
    collected from areas of high and low pollution. Jensen, et al. (1972b)
    found five times as much PCBs in herrings caught in waters off
    industrialized areas near Stockholm, as in herrings from the cleaner
    waters of the west coast of Sweden. Similarly, levels in plankton
    harvested along the Swedish archipelago at various distances from
    Stockholm fell progressively, away from the more polluted areas
    (Jensen, et al., 1972c); the concentration in pike fell to one-half
    (Olsson & Jensen, 1974). Koeman et al., (1972b) found PCB levels of up
    to 88 mg/kg in the blubber of toothed whales caught in the North Sea,
    but none was detectable in similar species from New Zealand or
    Surinam. Holden (1973b) found high PCB concentrations in the blubber
    of seals in polluted coastal areas of the United Kingdom (up to
    235 mg/kg), and much lower levels in unpolluted areas (down to
    2 mg/kg).

        Risebrough & de Lappe (1972) studied the PCB content of
    extractable lipids from brown pelican eggs collected from areas
    throughout North and South America, and showed that the content varied
    from 4 mg/kg up to 266 mg/kg in highly industrialized regions. They
    also reported levels greater than 3 mg/kg in fish from New York Sound
    and Tokyo Bay, both very polluted areas. Even higher levels of PCBs
    have been found in fish from polluted lakes and inland waterways, a
    level of 20 mg/kg being found in fish from Lake Ontario, and over
    200 mg/kg in fish from the Hudson River (Stalling & Mayer, 1972).
    Similar correlations between pollution and PCB levels have been
    reported from the United Kingdom in fish (Portmann, 1970), and in
    mussels (Holdgate, 1971).

        The association between high PCB levels and local pollution may be
    disturbed by the migratory habits of certain species, particularly in
    birds that may be exposed to PCBs in their wintering areas or on the
    migration routes. As much as 400 mg/kg has been measured in the fat of
    a robin entering Sweden, although the normal value is about 16 mg/kg.
    Many migratory birds start egg-laying on arrival at their summer
    quarters, so the PCB content of eggs may reflect the bird's previous
    exposure rather than the local pollution (Odsjö, 1973). A special case
    concerning the effect of pollution is seen in the use of fish-feed in
    poultry and fish farming. Kolbye (1972) stated that this may contain
    PCB levels of 0.6-4.5 mg/kg.

    5.4.2  The influence of the fat content of tissues

        PCBs are mainly stored in body fat (see section 5.5.5.1), and the
    total PCB content of the body tissues is much influenced by their fat
    content (Portmann, 1970; Westöö & Norén, 1970a). Jensen, et al. (1969)
    found PCB levels of 0.27 mg/kg and 0.33 mg/kg respectively, in the
    muscle tissue of herring and cod from the same area of the Baltic,
    although the cod is at a higher trophic stage (see section 5.4.3).
    These two species have 4.4 and 0.32% of extractable fat respectively,
    and when the PCB level is calculated on the fat content, values of
    6.8 mg/kg for the herring and 11 mg/kg for the cod are obtained. Cod
    liver has a much higher fat content than cod muscle and Jensen (1973)
    has reported the ratio of PCB concentrations in cod liver and muscle
    to be over 100, the maximum in liver being 59 mg/kg. Jensen et al.
    (1969) have remarked that the considerable seasonal variation in the
    fat content of the herring, rising from 1% in spring to 10% in autumn,
    influences the tissue level of PCBs. Peakall et al. (1972) noticed a
    marked rise in tissue levels in starved birds owing to the
    mobilization of fat, and it is possible that the high levels of PCBs
    in the livers of birds dying during the "seabird wreck" in the Irish
    Sea were secondary to emaciation (Holdgate, 1971). De Freitas &
    Norstrom (1974) showed that, in pigeons, pure PCBs left fatty tissues
    and accumulated mainly in muscle during the mobilization of fat
    associated with starvation.

    5.4.3  The influence of the trophic stage in food

        Swedish work on the distribution of PCBs in aquatic ecosystems has
    been summarized by Jensen et al. (1972b), and Olsson et al. (1973),
    and it has been largely confirmed by work in other areas (Risebrough
    et al., 1968; Risebrough & de Lappe, 1972: Holdgate, 1971). Zoo- and
    phytoplankton readily absorb or adsorb PCBs from their environment;
    Södergren (1972) has demonstrated the rapid uptake of Clophen A50 by
    the unicellular alga  Chlorella. Marine zooplankton may contain PCB
    levels of 5 mg/kg in extractable lipids in areas of moderate pollution
    (Jensen et al., 1972c; Williams & Holden, 1973), with somewhat lower

    values in relatively unpolluted areas. However, results with plankton,
    particularly where high levels are found, must be regarded with
    caution as the sample could be contaminated with PCB-rich oil or tar
    particles. Herring feeding on plankton in areas of moderate pollution
    contain PCB levels of about 0.5 mg/kg in muscle tissue (10 mg/kg in
    extractable fat); plaice and flounder, both bottom-feeding fish
    contain about one-third of this, presumably because the PCB content of
    benthic organisms is lower. In predatory fish such as the cod and
    pike, the PCB level in extractable fat is about 10 mg/kg and a mean of
    10 mg/kg has been measured in the blubber of seals.

        Much higher PCB values have been obtained in fish-eating birds;
    levels of 18 mg/kg (650 mg/kg in extractable fat), and 17 mg/kg
    (420 mg/kg in extractable fat) were found in the herring gull and
    comorant respectively. Lower values were found in birds feeding on
    invertebrates, such as the long-tailed duck which contained 14 mg/kg
    in fat; marine invertebrates contain PCB levels in the region of
    0.1-0.2 mg/kg. At the top trophic level, 96 mg/kg (9.7 g/kg in
    extractable fat) has been measured in the eagle owl; the highest
    recorded PCB values were from eagle owls found dead in the south-east
    coastal region of Sweden, 260 mg/kg in the brain (3.4 g/kg in
    extractable fat) and 110 mg/kg in muscle (12 g/kg in extractable fat).
    (Odsjö, 1973).

        Less information is available on terrestrial ecosystems. PCB
    concentrations in the region of 0.01 mg/kg have been found in fresh
    tissue in slugs, snakes, and ants, and slightly higher concentrations
    in earthworms. Tissue levels were generally at the limit of detection
    (0.01 mg/kg) in herbivorous mammals (Odsjö, 1973). Bruggemann et al.
    (1974) reported a mean PCB concentration of 0.22 mg/kg in 20 out of 72
    measurements in the adipose tissues of the hare and a higher value
    (2.5 mg/kg) in 1 out of 5 tests on the adipose tissues of the fox.
    Higher values have been found in the American mink in Sweden with
    0.58 mg/kg in muscle (45 mg/kg in fat) presumably because of a fish
    diet (Odsjö, 1973). Tissue levels in wild birds on a mixed diet are
    variable and rather low, but those in predatory birds are higher.
    Prestt et al. (1970) related the PCB concentration in the liver of
    wild birds to their diet; less than 1 mg/kg was found in insectivorous
    birds and more than 70 mg/kg in the sparrow hawk. A level of 0.5 mg/kg
    has been found in the muscle of the eagle owl in central Sweden, but
    this is much less than the tissue concentrations encountered in this
    bird in coastal areas (Odsjö, 1973). High tissue concentrations in
    predatory and marine birds have also been reported from Canada
    (Gilbertson & Reynolds, 1974), the Netherlands (Koeman et al., 1972a),
    and from the United Kingdom (Bourne & Bogan, 1972).

    5.4.4  Indicator organisms

        Several of the organisms, that have been shown to accumulate-PCBs
    from the environment, have been suggested as indicators of the extent
    of local pollution with PCBs. In aquatic systems, the use of plankton
    as an indicator has the advantage that it is at the lowest trophic
    stage of food chains but errors may occur in the determination because
    of the inclusion in the sample of nonplanktonic particles with a high
    PCB content (Jensen et al., 1972a). The herring, which feeds on
    plankton, has been suggested as an indicator (Jensen et al., 1972b)
    and, at higher trophic stages, the pike, which is a stationary fish
    (Olsson & Jensen, unpublished report, 1974), and seabirds (Jensen et
    al., 1972c). In fresh waters, the amphipod  Gammarus pulex has been
    used as an indicator organism for chlorinated hydrocarbons (Södergren
    et al., 1972). However, Zitko et. al. (1974) claimed that the
    variation between individual fish was so high that Atlantic herring
    and yellow perch could be used to detect trends in pollution only if
    large numbers were taken for analysis, with an interval between
    measurements of at least 4 years.

        A series of monitoring studies has been made by OECD, the species
    selected covering terrestrial, fresh water, and marine environments.
    The analytical results, and the general problems of selecting species
    for monitoring, have been discussed by Holden. (1970a, 1973a, 1973b).

    5.5  The Extent of Human Exposure to PCBs and PCTs

    5.5.1  Air and water

        The maximum concentration of PCBs in air is not likely to exceed
    50 ng/m3 (section 5.1). The highest concentration of PCBs reported in
    domestic tap water is 100 ng/litre in the Kyoto area of Japan (Panel
    on Hazardous Trace Substances, 1972), but levels more likely to be
    encountered should not exceed 1 ng/litre (section 5.3).

    5.5.2  Food

        The PCB content of a variety of foods on the Swedish market been
    measured by Westöö & Norén (1970a) and Westöö et al. Less than
    0.1 mg/kg was found in samples of butter, margarine, oils, eggs, beef,
    lamb, chicken, bread, biscuits, and baby of pork out of more than 100
    had a PCB content in the range of 0.5 mg/kg. As might be expected from
    the discussion in section 5.4 higher values were found in fish
    depending on the fat content and pollution of the fishing area (Westöö
    & Norén, 1970a; Berglund, 1972] The PCB levels obtained in an
    extensive study by the US Food Administration are shown in Table 5.

        These values are considerably higher than but they are probably
    biased, as they include samples originating areas previously suspected
    of having been subject to local pollution. In a Canadian survey, PCB
    levels of less than 0.01 mg/kg were found in eggs (Mes et al., 1974)
    and a mean of 0.042 mg/kg was found in domestic and imported cheese
    with a maximum of 0.27 mg/kg (Villeneuve et al., 1973b). No traces of
    PCTs were found.

        In Japan, a similar range of PCB contents for most foods has been
    reported; however, some high levels have been-reported for rice and
    vegetables harvested in fields polluted with PCBs (Environmental
    Sanitation Bureau, 1973). The PCB content of most fish on the market
    was less than 3 mg/kg, although some contained more than this. The PCT
    content of fish was much lower (Fukano et al., 1974). In the
    Netherlands, eel has been reported to contain PCT levels of
    0.2-0.5 mg/kg and PCB levels of 4.7 mg/kg (Freudenthal & Greve, 1973).

    Table 5.  PCB levels in food in the USAa
                                                                         

                  % positive        Level in positive samples (mg/kg)
    Food          (0.1 mg/kg)           Mean              Maximum
                                                                         

    Cheese            6                 0.25              1.0
    Milk              7                 2.3               27.8
    Eggs             29                 0.55              3.7
    Fish             54                 1.87              35.3
                                                                         

    a  From Kolbye (1972).

        Samples of butter from the Westphalian area of the Federal
    Republic of Germany, obtained in the period 1972-74, contained PCB
    levels of 0.38 mg/kg (range 0.25-0.54 mg/kg) (Claus & Acker, 1975).

        Relatively high PCB levels in some packaged foods in Sweden,
    mainly of imported origin, could be attributed to migration from the
    packaging material (Westöö et al., 1971). The highest level
    encountered was 11 mg/kg in a children's breakfast cereal; PCB levels
    of 70 mg/kg and 700 mg/kg were found in the material of the inner bag
    containing this product and in the outer cardboard container
    respectively. Up to 2000 mg/kg was found in cartons of other samples.
    Villeneuve et al. (1973a) have analysed packaged food in Canada; they
    found that 66.7% of the samples contained PCB levels of less than
    0.01 mg/kg, 30.7% contained between 0.01 and 1 mg/kg, and 2.6%
    contained more than 1 mg/kg. PCT determinations were also made on
    these samples; 94.5% of the samples contained less than 0.01 mg/kg and

    5.5% contained 0.01-0.05 mg/kg. The highest PCB levels were
    encountered in a rice sample with 2.1 mg/kg where the packaging
    material contained 31 mg/kg, and in a dried fruit sample with
    4.5 mg/kg in a container containing 76 mg/kg. In a survey of packaging
    containers, approximately 80% were found to contain PCB and PCT levels
    of less than 1 mg/kg, while about 4% contained levels higher than
    10 mg/kg. The most likely source of PCBs in packaging materials is the
    recycling of paper waste containing pressure-sensitive duplicating
    paper (Masuda et al., 1972).

    5.5.3  Occupational exposure

        Occupational exposure does not only occur during the manufacture
    of PCBs and with their use in the electrical industry. It may also be
    widespread among mechanics in contact with lubricating oils and
    hydraulic fluids, among workers exposed to varnishes and paints, and
    among office workers from contact with pressure-sensitive duplicating
    paper, some brands of which readily transfer PCBs to skin (Kuratsune &
    Masuda, 1972b). Studies in Finland showed that whole blood from
    persons with no special exposure to PCBs contained 0.3-1.2 µg/100 ml,
    while blood from persons handling PCBs in an analytical laboratory
    contained 3.66.3 µg/100 ml and blood from workers in a capacitor
    factory had PCB levels of 7.5-190 µg/100 ml in the blood and
    30-700 mg/kg in fat. No signs of toxicity were evident in these workers
    (Karppanen & Kolho, 1973). Similar plasma values were found in workers
    from Japanese capacitor factories, but here skin lesions were noted
    (Hasegawa et al., 1972a). This same study reported that air levels of
    PCBs of 0.01-0.05 mg/m3 were measured in a factory where KC-300 was
    used in the manufacture of electric condensers. PCB levels in serum in
    workers ranged from 10 to 65 µg/100 ml.a One month after the use of
    PCBs had been suspended, serum levels still ranged from 9 to
    74 µg/100 ml. However, in another factory making electric condensers, serum
    levels decreased from an average of 80 µg/100 ml to 30 µg/100 ml
    within three months of the use of PCBs being suspended (Kitamura et
    al., 1973). According to Hara et al. (1974), the half-time of PCBs in
    the blood of workers engaged in the manufacture of electric condensers
    for less than 5 years was several months, while that of workers
    employed for more than 10 years was 2-3 years. Hammer et al. (1972)
    found a higher frequency of measurable plasma values in workers
    working with refuse burners than in a control group.

                 

    a  In this document, the concentrations of PCBs in blood and serum
       are expressed in µg/100 ml although in some original papers the
       values are given in µg/100 g. For practical purposes the
       differences, about 5% and 3% respectively, can be neglected.

    5.5.4  Other sources of exposure

        Broadhurst (1972) has reviewed the many technical applications of
    PCBs that appear in the literature and in patent specifications, and
    which indicate the possibility of a widespread nonoccupational
    low-level exposure to PCBs, other than that deriving from the diet.
    PCBs are used in the home in ballast capacitors for fluorescent
    lighting, and exposure deriving from pressure-sensitive copying paper
    has not been limited to office workers. The valuable properties of
    PCBs as plasticizers has led to their use in furnishings, interior
    decoration, and building construction; examples are surface treatment
    for textiles, adhesive for waterproof wall coatings, paints, and
    sealant putties. PCBs have been used as plasticizers for plastic
    materials and in the formulation of printing inks.

    5.5.5  Biological indices of human exposure

        The only surveys of value have been on body fat, blood, and milk.

    5.5.5.1  Body fat

        In a survey of 637 fat samples taken at autopsy or during surgery
    in the USA, 68.9% contained PCB levels of less than 1 mg/kg, 25.9%
    contained 1-2 mg/kg, and 5.2% contained more than 2 mg/kg (Yobs,
    1972). A similar distribution was found in a smaller survey by Price &
    Welch (1972). In the Kochi area of Japan, a mean PCB level of
    2.86 mg/kg was recorded with an upper limit of 7.5 mg/kg; about double
    these values were found in the Kyoto area (Nishimoto et al., 1972a,
    1972b). Bjerk (1972) reported average PCB levels of 0.9 mg/kg
    (1.6 mg/kg on a lipid basis) in adipose tissue, taken at 40 autopsies
    in the Oslo area. Curley et al. (1973b) found PCB levels ranging from
    0.30 to 1.48 mg/kg in a total of 241 human adipose samples in Japan. A
    mean value of 5.7 mg/kg has been reported for 20 samples from the
    Federal Republic of Germany (Acker & Schulte, 1970); in a more recent
    study, 282 adipose tissue samples from different areas were found to
    contain PCB levels of 8.3 mg/kg of adipose tissue on a lipid basis
    (Acker & Schulte, 1974). In Austria, a range of PCB levels of
    0.3-7.3 mg/kg on a lipid weight basis was found in 32 residents in the
    Vienna metropolitan area; an increase of the PCB concentration with
    age was not observed (Pesendorfer et al., 1973). Detectable levels of
    PCBs were found in only a few of 201 human fat samples in the United
    Kingdom and in these, the level did not exceed 1 mg/kg (Abbott et al.,
    1972). A survey of 51 human fat samples in New Zealand showed that all
    samples contained PCB residues with an average of 0.82 mg/kg (Solly &
    Shanks, 1974).

        Doguchi et al. (1974) found an average PCT level of 0.6 mg/kg in
    human fat with a range of 0.1-2.1 mg/kg. Takizawa & Minagawa (1974)
    also found PCT levels of 0.02 mg/kg in human liver ( n = 6),
    0.01 mg/kg in the kidney ( n = 2), 0.02 mg/kg in the brain ( n = 3)
    and 0.04 mg/kg in the pancreas ( n = 1). In the Netherlands, PCTs
    were found in human fat at levels of 0-1 mg/kg (Freudenthal & Greve,
    1973).

    5.5.5.2  Blood

        43% of blood plasma samples from 723 volunteers in the USA showed
    the presence of PCBs; the mean value in these was about 0.5 µg/100 ml
    (Finklea et al., 1972). Studies in Finland on whole blood showed
    0.31-1.2 µg/100 ml in persons with no special exposure to PCBs
    (Karppanen & Kolho, 1973). In Japan, an average PCB level of
    0.32 µg/100 ml and a PCT level of 0.5 µg/100 ml have been recorded in
    the blood of non-occupationally exposed volunteers (Doguchi & Fukano,
    1975). Inpatients with severe weight loss, high levels of PCBs in the
    blood (up to 10 µg/100 ml) were noted (Hesselberg & Scherr, 1974).
    This was attributed to the release of PCBs from the mobilization of
    fat.

    5.5.5.3  Human milk

        PCB concentrations measured in whole human milk in Sweden were
    0.014 mg/litre in 1967 and 0.025 mg/litre in 1971-72 (Westöö & Norén,
    1972), 0.03 mg/litre in Japan Nishimoto et al., 1972a), 0.103 in the
    Federal Republic of Germany (Acker & Schulte, 1970) and 0.02 mg/litre
    in Canada (Musial et al., 1974) A survey in Colorado, USA, revealed 8
    positive samples out of 39, within the range of 0.04-0.1 mg/kg (Savage
    et al., 1973).

    5.5.6  Estimated daily intake

        From the PCB levels encountered in air and drinking water (section
    5.5.1), the daily intake from each of these sources is likely to be
    less than 1 µg.

        It has been stated that the major part of the human dietary intake
    of PCBs is from fish (Berglund, 1972; Hammond, 1972). This may well be
    true in areas such as Japan or certain localities near the North
    American Great Lakes, where fish from polluted waters may form a
    relatively large part of the diet. Several investigators from Japan
    have measured the daily intake of PCBs in food; the highest mean value
    recorded was 48 µg/day, of which 90% was from fish (Kobayashi, 1972);
    the lowest was 8 µg/day (Ushio et al., 1974).

        In much of Europe and North America, however, the daily intake of
    fish is in the region of 30-40 g and most of the fish is taken from
    waters of low pollution and contains PCB levels of not more than
    0.1 mg/kg. Berglund (1972) has estimated that the daily intake of PCBs
    from fish in Sweden is in the region of 1 µg, though if the fish
    consumed were solely Baltic herring, the intake would be about 10 µg.
    It is difficult to make an assessment of the PCB intake from foods
    other than fish. Westöö et al. (1971, 1972), in their extensive study
    of the Swedish diet, reported that most foods contained PCB levels of
    less than 0.1 mg/kg; it may be concluded that this corresponds to a
    daily intake of less than 100 µg. The conclusion that foods other than
    fish may make a greater contribution to the PCB content of the diet
    can be drawn from the survey in the USA reported by Kolbye (1972), and
    also from a Swiss study of three different types of home-prepared
    meals, not containing fish, which were found to contain 6, 41, and
    84 µg of PCBs, respectively (Zimmerli & Marek, 1973).

        The figures for the PCB content of human milk (section 5.5.5.3)
    indicate that most nursing mothers excreted about 30 µg/day by this
    route, and in some areas up to 100 µg/day. It may be assumed that only
    a portion of the PCBs absorbed was excreted by this route, so that the
    daily intake could have been more than 100 µg.

        It may be concluded that, in the more industrialized countries,
    the average daily PCB intake from the diet has rarely been less than
    5 µg or greater than 100 µg; it is likely that the non-dietary sources
    of exposure detailed in section 5.5.4 have made a significant
    contribution, but this cannot be estimated at this time. In any area,
    the intake depends not only on the diet, but also on social domestic
    and environmental conditions; the influence of these factors on the
    daily intake cannot readily be quantified.

    6.  METABOLISM

    6.1  Absorption

        The experiments of Vos & Beems (1971) who applied several
    commercial PCBs to rabbit skin and found systemic effects (see section
    7.1.1.4) indicate that PCBs can penetrate the skin. Early cases of
    human poisoning from occupational exposure were probably due to a
    combination of skin absorption and inhalation. Experimental studies on
    rats by Benthe et al. (1972a) showed that an aerosol containing
    Aroclor 1242 (particle size 0.5-3.0 µm) was readily absorbed through
    the lungs.

        Although one means of entry of PCBs into aquatic food chains is
    through the consumption of plankton by fish, aquatic organisms can
    also absorb PCBs in solution in the ambient water, presumably mainly
    through the gills (see section 4.3). Salmon eggs can also absorb PCBs
    from water (Johansson et al., 1970), and Södergren & Svensson 1973
    concluded that mayfly nymphs could take in PCBs from water through the
    gills and the integument.

        Recent work with chlorobiphenyl isomers administered orally to
    rodents at levels up 100 mg/kg of body weight for lower chlorinated
    compounds and up to 5 mg/kg for the higher chlorinated compounds,
    showed that 90% of the compounds were rapidly absorbed (Albro &
    Fishbein, 1972; Berlin et al., 1973; Melvås & Brandt, 1973).
    Cholestyramine, a basic anion exchange resin, was shown to interfere
    with intestinal absorption of KC-400 in mice (Tanaka & Araki, 1974).

        PCTs have been shown to be absorbed from the gut (Sosa-Lucero et
    al., 1973) but very little information is available on the rate of
    absorption.

    6.2   Tissue Distribution of PCBs

        Grant et al. (1971a) demonstrated that 4 days after an oral dose
    of Aroclor 1254 at 500 mg/kg was given to rats, the concentrations of
    PCBs in fat, liver, and brain were 996, 116, and 40 mg/kg,
    respectively. Similar results showing that the highest concentration
    was in fat, were obtained in rats given Aroclor 1254 in the diet
    (Curley et al., 1971), in boars (Platonow et al, 1972), cows (Platonow
    & Chen, 1973), and in pigeons and quail (Bailey & Bunyan, 1972). In
    the experiments of Curley et al. (1971), the tissue concentrations
    initially showed a rapid rise and thereafter a slow increase while the

    PCB diet was being administered; Grant et al. (1974) fed diets
    containing Aroclor 1254 at 0.2, 20, and 100 mg/kg to rats for 8
    months, during which period the tissue concentrations reached a steady
    state that was dose-dependent (Table 6). Similar tissue distribution
    data for Aroclors 1016 and 1242 have been reported by Burse et al.
    (1974) and for Kanechlor-400 by Yoshimura et al. (1971). PCB
    deposition, in general, depends on the fat content of the tissue
    (section 5.4.2). Residues in trout, receiving Aroclor 1254 in doses of
    15 mg/kg in the diet, stabilized after 16 weeks while the absolute
    quantity continued to increase as the fish grew (Lieb et al., 1974).

        More detailed information on the tissue distribution of PCBs and
    their metabolites has been obtained by the administration of pure
    14C-labelled compounds, using both whole-body autoradiography and
    scintillation counting of tissue samples. Berlin et al. (1975)
    demonstrated that after a single oral dose of 14C-labelled
    2,5,2',4',5'-pentachlorobiphenyl, radioactivity rapidly entered the
    circulation of mice and was distributed in the tissues, particularly
    in the liver, kidneys, lungs, and adrenals. Subsequently, the
    radioactivity in the body fat increased, rising to a maximum within
    4-24 h. In most other tissues the radioactivity decreased rapidly
    after dosing, but the authors noted a special affinity for the skin,
    the bronchiolar epithelium of the lungs, and certain glandular
    secreting tissue. Soon after administration of the dose, radioactivity
    appeared in bile and was excreted in the faeces. Similar results were
    obtained by Melvås & Brandt (1973) with 2,4,2',4'-tetrachlorobiphenyl
    in the mouse, and with 2,4,2',3'- and 2,4,3',4'-tetrachlorobiphenyls
    in the quail; in the mouse they found a high affinity for the adrenal
    cortex, the corpora lutea, and glandular secreting tissue, and in the
    quail the radioactivity in egg yolk was high, exceeding that in fat.
    Brandt & Ullberg (unpublished report 1973) found a similar pattern
    after administration of hexa- and octachlorobiphenyls to mice.

    6.3  Tissue Distribution of PCTs

        Diets containing Aroclor 5460 at levels of 10, 100, and 1000 mg/kg
    were administered to rats for 7 days (Sosa-Lucero et al., 1973). Table
    7 shows the tissue distribution obtained in this study in rats fed
    with Aroclor 5460 at 100 mg/kg body weight and the values in rats fed
    with Aroclor 1254 at 100 mg/kg body weight in a similar study (Curley
    et al., 1971). After oral administration of Aroclor 5460 to the cod,
    the concentration of PCTs in the liver was more than 100 times that in
    muscle on a wet weight basis (Addison et al., 1972), a ratio found by
    Jensen et al. (1973) for PCBs in the cod.

    Table 7.  Tissue distribution (mg/kg wet weight) of PCTs (Aroclor
              5460) in rats fed dietary levels of 100 mg/kg for 7 days
              (Sosa-Lucero et al., 1 973) and fed PCB (Aroclor 1254) at
              100 mg/kg for 9 days (Curley et al., 1971)
                                                                         

                          Aroclor                Aroclor
    Tissue                5460                   1254
                                                                         

    Blood                   1.32                   0.1
    Liver                  47                      6
    Brain                   5.1                    4
    Kidneys                15.1                    5
    Heart                  21.5                   --
    Fat                    --                    180
                                                                         

    6.4  Placental Transport

        Aroclors 1221 and 1254 were found to cross the placenta of
    rabbits, when administered orally to does during gestation. The
    concentration in fetal tissues was dose-dependent and much less with
    Aroclor 1221 than with Aroclor 1254; with the latter, the
    concentration in the fetal liver was greater than that in the maternal
    liver (Grant et al., 1971b). Curley et al. (1973a) found some
    placental transport of Aroclor 1254 in the rat. Platonow & Chen (1973)
    demonstrated that the PCBs in the fetal kidney of a cow dosed with
    Aroclor 1254 was greater than that in the mother. Placental transfer
    of polychlorinated biphenyls has also been reported in the mouse
    (Berlin et al., 1975; Melvås & Brandt, 1973; Brandt & Ullberg,
    unpublished report 1973).

        PCB concentration in human umbilical blood has been shown to be
    about 25% of that in maternal blood (Taki et al., 1973). Placental
    transfer of PCBs was observed in Yusho patients (Tsukamoto et al.,
    1969).

        No information is available on the placental transfer of PCTs.

    6.5  Excretion and Elimination

    6.5.1  Milk

        Saschenbrecker et al. (1972) found that after oral administration
    of doses of Aroclor 1254 of 10, and 100 mg/kg in diet to cows, 6.27
    and 74.5 mg/litre, respectively, appeared in the milk after 24 hours.
    These levels were reduced to less than one-half within 3 days but
    traces remained at 50 days. Cows receiving 200 mg of Aroclor 1254
    daily reached a steady state concentration of 61 mg/kg in milk fat and
    42 mg/kg in body fat after 10 days (Fries et al., 1973). The PCBs in
    milk survived processing into dairy products, and most was located in
    milk fat (Platonow et al., 1971). PCBs have also been found in human
    milk (see section 5.5.5.3).

    6.5.2  Eggs

        Several investigations have demonstrated the presence of high
    levels of PCBs in the eggs of seabirds (Riseburgh & de Lappe, 1972).
    An incident has been reported in which PCB contamination of poultry
    food was toxic to chickens and decreased the hatchability of eggs
    which also contained PCB residues (Pichirallo, 1971). In a laboratory
    study, Scott et al. (1975) fed Aroclor 1248 to chickens at dietary
    concentrations of 0, 0.5, 1.0, 10, and 20 mg/kg. After 8 weeks, the
    approximate PCB levels in eggs were 0, 0.22, 0.41, 3.1 and 7.0 mg/kg
    respectively. Jensen & Sundström (1974) found 33% of ah oral dose of
    2,4,5,T,4',5'-hexa-chlorobiphenyl administered to quails was excreted
    in eggs over a period of 10 days; eggs also provided a major route of
    excretion in the pheasant (Dahlgren et al., 1971).

    6.5.3  Urine and faeces

        All investigators agree that only traces of PCBs can be found in
    the urine of dosed animals, and that faeces provide a major route of
    elimination. When the analysis of faeces is limited to the
    determination of unchanged PCBs, the recovery of the dose administered
    is incomplete; in boars receiving single or repeated doses of Aroclor
    1254, not more than 16% of the dose was recovered from the faeces and
    less than 1% in urine (Platonow et al., 1972). Better recoveries have
    been obtained with PCB labelled with radioactive isotopes. Yoshimura
    et al. (1971) found 70% of the activity from a dose of tritium-
    labelled Kanechlor 400 in faeces and 2% in urine over a 4-week period.
    Berlin et al. (1974, 1975) found over 75% of the activity from
    14C-labelled penta- and hexachlorobiphenyls in faeces and less than
    2% in urine; most of the faecal excretion consisted of PCB metabolites
    (see section 6.6.1). Similar results were obtained by Melvås & Brandt
    (1973) with tetrachlorobiphenyls.

    6.6  Biotransformation

    6.6.1  Metabolic degradation

        Most investigators studying the tissue distribution of PCBs after
    administration of commercial mixtures have noted a relative reduction
    of the gas-liquid chromatographic peaks with shorter retention times,
    corresponding to the lower chlorinated biphenyls. This has been
    reported in the rat (Grant et al., 1971a; Curley et al., 1971), the
    rabbit (Grant et al, 1971b), the cow (Platonow & Chen, 1973), and in
    pigeons and quails (Koeman et al., 1969; Bailey & Bunyan, 1972).
    Samples of tissues from animals and man (see table 3, pp. 20-21) that
    have absorbed PCBs from the environment have shown, on analysis, a
    pattern of peaks approaching that of PCB mixtures with more than 50%
    chlorination, although the major manufactured products contain 42% of
    chlorine or less. This has led to the belief that the rate of
    metabolic attack on PCBs decreases with increasing chlorination.
    Studies on single PCBs with 1, 2, 4, or 5 chlorine atoms have shown
    that these are more readily excreted as metabolites in faeces by
    mammals and birds and remain for a shorter time in fatty tissues than
    most PCBs with 6 or more chlorine atoms (Berlin et al., 1975;
    Hutzinger et al., 1972a; Melvås & Brandt, 1973; Brandt & Ullberg,
    unpublished report 1973). See also section 6.6.2.

        The administration to rats of diets containing Aroclors 1016 or
    1242 at a concentration of 100 mg/kg resulted in a steady state in
    adipose tissue for both compounds in about 4 months. When gas
    chromatographic traces were compared with those for standard PCB
    mixtures a difference in the gas-liquid chromatographic pattern, that
    is disappearance of the peaks with short retention times was noted.
    After exposure to PCBs was discontinued, a major portion of the PCBs
    was eliminated from the body in 4 months. However, 20% of the total
    PCBs in Aroclor 1242 with longer retention times were present after 6
    months, and 10% of the total PCBs of Aroclor 1016 with longer
    retention times were present in adipose tissue after 5 months. (Burse
    et al., 1974).

        The excretion of monohydroxy metabolites of 3,4,3',4'-tetrachloro-
    biphenyl and 2,4,3',4'-tetrachlorobiphenyl (orally administered) in
    rats has been demonstrated by Yoshimura & Yamamoto (1973). Yoshimura
    et al. (1973), Yamamoto & Yoshimura (1973), Yoshimura & Yamamoto
    (1974), and Yoshimura et al. (1974). They demonstrated that the
    metabolites of the first isomer were 2-hydroxy or 5-hydroxy compounds
    while the metabolites of the second isomer were 5-hydroxy and
    3-hydroxy compounds. All hydroxy metabolites were excreted
    non-conjugated via the bile and no parent isomers were found in the
    bile. Yoshimura & Yamamoto (1975) found that unchanged
    2,4,3',4'-tetrachlorobiphenyl was excreted through the intestine, when
    it was intravenously injected in rats with the bile duct ligated,
    while no metabolite of this isomer was excreted by this route.

        Hutzinger et al. (1972a) demonstrated the presence of hydroxylated
    derivatives of mono-, di-, and tetrachlorobiphenyls in the excreta of
    rats and pigeons but not of trout and were unable to detect
    hydroxylated 2,4,5,2',4',5'-hexachlorobiphenyl. Berlin et al. (1975)
    isolated a hydroxy derivative of 2,5,2',4',5'-pentachlorobiphenyl from
    mouse faeces, and Jensen & Sundström (1975) have shown that although
    2,4,5,2',4',5'-hexachlorobiphenyl is excreted very slowly, a hydroxy
    derivative could be detected in rat faeces. Hutzinger et al. (1974),
    however, showed that this compound was also dechlorinated by the
    rabbit and excreted as the hydroxy derivative of pentachlorobiphenyl.
    Gardner et al. (1973) detected hydroxylated metabolites in the urine
    of rabbits dosed with 2,5,2',5'-tetrachlorobiphenyl together with a
    dihydroxy derivative which they regarded as evidence for the formation
    of an arene oxide (epoxide) intermediate (see section 6.5.3). Jansson
    et al. (1975) have identified up to 26 mono- and dihydroxylated
    metabolites of PCB in the bile and faeces of wild grey seal and
    guillemot from the Baltic.

        There is little information on the biotransformation of PCTs.
    Addison et al. (1972), using gas-liquid chromatography, noted a loss
    of PCTs with a shorter retention time in the excreta of a cod dosed
    orally with Aroclor 5460; the same loss was observed in rat faeces
    after the administration of a diet containing Aroclor 5460
    (Sosa-Lucero et al., 1973).

    6.6.2  The effect of structure on retention

        While each of the components of mixtures of PCBs has a different
    pattern of retention and elimination in different species, the
    measurement of biological half-times for PCB mixtures in tissues has
    provided useful information. Bailey & Bunyan (1972) found the
    half-time of PCBs in the fat of quail and pigeon, after cessation of
    dosing with Aroclor 1242, or with Aroclor 1254, to be 50 days and 125
    days, respectively. A half-time of about 200 days was recorded in the
    fat of rats after feeding with Aroclor 1254 (Grant et al., 1974).
    Berlin et al. (1975) noted that in mice dosed with a
    pentachlorobiphenyl there was an initial fairly rapid elimination
    while the PCB level in the liver was high, followed by a slower
    elimination when most of the PCB was located in fat. It seems likely
    that the mobilization of PCBs from fat, and therefore their half-time
    in the body, depends upon their rates of metabolism. Berlin et al.
    (1974) investigated the hypothesis that the ability of a PCB to be
    readily degraded with a half-time of a few days depended upon the
    presence of two adjacent unsubstituted carbon atoms in the molecule

    rather than on the number of chlorine atoms, although the presence of
    such unsubstituted pairs depends to a large extent on the degree of
    chlorination. They came to the conclusion that this hypothesis
    probably applied to unsubstituted pairs in the 3,4-position, but that
    in the 2,3-position, their susceptibility to metabolic degradation was
    much influenced by the presence of chlorines in the  o-position of
    the ring bridge.

        Jensen & Sundström (1974) demonstrated that the retention of PCBs
    in human fat is also influenced by  o-chlorine substitution (Table 3,
    pp. 20-21 ).

    7.  EXPERIMENTAL STUDIES ON THE EFFECTS OF PCBs AND PCTs

    7.1  Toxic Effects in Different Species

        Most of the available information on the toxicity of the PCBs has
    been obtained from studies on commercial mixtures. The much smaller
    amount of information available concerning the impurities in
    commercial products and PCTs, is given in sections 7.2 and 7.3.

    7.1.1  Mammals

    7.1.1.1  Acute oral and intravenous toxicity

        Earlier work by the Monsanto Company indicated a low acute oral
    toxicity for the Aroclors, the LD50s to rats ranging from 4.0 g/kg
    for Aroclor 1221 to 11.3 g/kg for Aroclor 1262 (Panel on Hazardous
    Trace Substances, 1972). More recent work has demonstrated a slightly
    higher toxicity (Bruckner et al., 1973; Grant & Philips, 1974). With a
    single intravenous dose the LD50 for Aroclor 1254 was 358 mg/kg body
    weight in adult female Sherman rats (Linder et al., 1974). The acute
    oral LD50 for Aroclor 1254 in the same strain and sex was between 4
    and 10 g/kg (Kimbrough et al., 1972). According to Bruckner et al.,
    (1973), severely poisoned animals showed weight loss, ataxia,
    diarrhoea, and chromodacryorrhoea, and they considered progressive
    dehydration and central nervous depression were the causes of death.
    In rats, vacuolation in the liver and kidneys was observed (Bruckner
    et al., 1973) and also ulceration of the gastric and duodenal mucosa
    (Kimbrough et al., 1972).

        Yamamoto & Yoshimura (1973) found the intraperitoneal LD50 of
    2,4,3'4'-tetrachlorobiphenyl in mice to be 2.15 g/kg body weight and
    that of the 5-hydroxy derivative, which is the main  in vivo
    metabolite, to be 0.43 g/kg body weight.

    7.1.1.2  Subacute oral toxicity

        After repeated administration, the PCBs have a cumulative toxic
    action. In a group of rats receiving Aroclor 1254 at a dose of 1 g/kg
    of diet, deaths occurred between the 28th and 53rd days of feeding
    (Tucker & Crabtree, 1970), and with Phenochlor DP6 at 2 g/kg of diet,
    deaths occurred between the 12th and 26th days (Vos & Koeman, 1970).
    In the latter experiment enlarged livers, small spleens, and a
    progressive chemically-induced hepatic porphyria were seen at autopsy.
    Repeated weekly oral administration of 150 mg of Aroclors 1221, 1242
    or 1254 to rabbits for 14 weeks produced liver enlargement and damage

    with Aroclor 1242 and no effect with Aroclor 1221 (Koller & Zinkl,
    1973). Allen et al. (1974) administered diets containing Aroclor 1248
    at the concentration of 25 mg/kg of diet to 6 female rhesus monkeys
    for two months; facial oedema, loss of hair, and acne developed after
    1 month and one animal died with severe gastritis 2 months after
    removal from experimental diet. PCB concentrations in the body fat of
    the animals, after two months of treatment, averaged 127 mg/kg while 8
    months later the value declined to 34-mg/kg.

        Mink appear to be unusually sensitive to PCBs. Aulerich et al.
    (1973) administered diets containing PCB levels of 30 mg/kg (10 mg/kg
    each of Aroclors 1242, 1248, and 1254) to adult mink and demonstrated
    100% mortality within 6 months. PCB residues in the brains of these
    mink averaged about 11 mg/kg and were approximately twice the level
    observed in other tissues. Four months of feeding Aroclor 1254 at
    levels of 5 and 10 mg/kg demonstrated a dose-dependent retardation of
    weight gain of growing female mink. Female mink fed a diet
    supplemented with Aroclor 1254 at 5 mg/kg for 9 months failed to
    produce offspring (Ringer et al., 1972).

    7.1.1.3  Chronic oral toxicity

        Aroclors 1242, 1254, and 1260 have been administered for 18 months
    to rats at 1, 10 and 100 mg/kg in the diet (Keplinger et al., 1971).
    No adverse effects were recorded with the three Aroclors at 10 mg/kg
    but with Aroclors 1242 and 1254 at 100 mg/kg there was an increase in
    liver weight and a reduced survival of litters. In similar experiments
    on dogs, there was a reduced weight gain with Aroclors 1254 and 1260
    in the diet at a level of 100 mg/kg (Keplinger et al., 1971). In the
    experiments reported by Kimbrough et al. (1972), male rats survived
    Aroclor 1260 in the diet at 1 g/kg (71.4 mg/kg body weight) for 8
    months but 8/10 females died at this dose, 2/10 died at 500 mg/kg, and
    1/10 at 100 mg/kg (7.2 mg/kg body weight). With Aroclors 1254 and
    1260, a dose-dependent increase in liver weight in male rats was
    significant down to 20 mg/kg (1.4 mg/kg body weight) in the diet; with
    females the liver enlargement occurred only at diet levels of
    500 mg/kg and higher. The livers showed an orange fluorescence, the
    cells were enlarged and vacuolated with lipid inclusions; there was
    also much increased smooth endoplasmic reticulum, and what was termed
    "adenofibrosis" was present, being more marked with Aroclor 1254 (see
    section 7.8). Grant et al. (1974) fed diets containing Aroclor 1254 at
    0, 2, 20, and 100 mg/kg to rats for 246 days followed by 180 days on a
    PCB-free diet; after 246 days the body weight of the 100 mg/kg rats
    was significantly less than that of the controls and the liver weight
    was greater. The most notable change in the livers on histological
    examination was the appearance of fat microdroplets in the
    centrilobular region; this effect was dose-dependent and not seen in
    the rats receiving the 2 mg/kg diet and was reversible when the rats
    were returned to a normal diet.

        Liver enlargement has been described by a number of authors, in a
    number of species, with different PCB mixtures and pure isomers and it
    is considered to be due primarily to hypertrophy of the smooth
    endoplasmic reticulum of the liver cells (Vos & Beems, 1971; Allen et
    al., 1973; Kimbrough, 1974; Nishizumi, 1970) but with large enough
    doses (oral intubation of about 150 mg/kg body weight/week for 14
    weeks) of Aroclor 1254 and 1242, it progressed to frank liver damage
    (Koller & Zinkl, 1973). The smooth endoplasmic reticulum may condense
    in the liver cell and form hyalin inclusions and this may be
    accompanied by a loss of enzyme activity. Lipid accumulation, pigment
    deposition, nuclear changes, and necrosis may also occur (Vos &
    Notenboom-Ram, 1972).

        The rhesus monkey is the only species reported to show signs of
    poisoning similar to those in human Yusho patients (see section 8).
    The administration of Aroclor 1248 at 2.5 and 5.0 mg/kg of diet for 1
    year produced periorbital oedema, alopecia, erythema, and acneiform
    lesions involving the face and neck within 1-2 months. The effects
    were less marked in male monkeys. At 25 mg/kg of diet, one out of a
    group of six died, and at 100 and 300 mg/kg the mortality approached
    100% within 2-3 months. Animals more severely affected showed
    hypertrophic hyperplastic gastritis with ulceration, anaemia,
    hyperproteinaemia and bone marrow hypoplasia. The survivors still
    showed signs of poisoning 8 months after exposure (Allen & Norback,
    1973; Allen et al., 1974; Allen 1975).

    7.1.1.4  Dermal toxicity

        Vos & Beems (1971) have confirmed earlier reports that PCBs damage
    the follicular epithelium in experimental animals. They applied three
    commercial 60% chlorinated mixtures, Clophen A60, Phenochlor DP6, and
    Aroclor 1260 to rabbit skin at a daily dose of 118 mg/50 cm (5 times
    per week) for 38 days. After initial reddening, transverse wrinkling
    developed with hyperplasia and hyperkeratosis of the epidermal and
    follicular epithelium. These effects were more marked with Clophen and
    Phenochlor than with the Aroclor.

        During these experiments, deaths occurred in the Clophen- and
    Phenochlor-treated groups but not in the Aroclor group. Kidney lesions
    were seen in all groups; liver damage was least in the Aroclor group.
    There was atrophy of the thymus cortex and a reduction of germinal
    centres of the lymph nodes as well as lymphopenia, and some animals in
    all groups showed oedema of the abdominal and thoracic cavities,
    subcutaneous tissue, and pericardium. Faecal excretion of copro- and
    protoporphyrins was increased by all three PCBs but was lowest with
    Aroclor 1260. Vos & Beems attributed the greater severity of the

    effects observed with Clophen and Phenochlor to the presence of toxic
    impurities (see section 7.2). In another comparative dermal toxicity
    study in rabbits (Vos & Notenboom-Ram, 1972), skin lesions in Aroclor
    1260-treated animals were more severe than in animals treated with
    2,4,5,2',4',5'-hexachlorobiphenyl. In a Japanese study, no differences
    in skin lesions were observed in rabbits after dermal applications of
    Kanechlor 400, Kanechlor 500, or 3,4,3',4'-tetrachlorobiphenyl
    (Komatsu & Kikuchi, 1972).

    7.1.1.5  Inhalation toxicity

        Only one inhalation study has been reported using Aroclor 1242 and
    1254 (Treon et al., 1956). Rats, mice, rabbits, and guinea pigs were
    exposed to Aroclor 1242 or 1254 vapours for five days a week for
    several weeks at concentrations ranging from 1.5 to 8.6 mg/m3. At
    these concentrations Aroclor 1254 produced liver enlargement in rats.

    7.1.2  Birds

        The acute oral toxicity of Aroclors 1242 and 1254 is low for the
    mallard duck, the LD50s being greater than 2 g/kg body weight (Tucker
    & Crabtree, 1970). Published figures for the lethal dose for birds
    after repeated administration are very variable; it appears to be
    dependent on the species and age of the bird, the method of
    administration, the degree of chlorination of the PCBs, and the
    presence of impurities (Vos, 1972). Heath et al. (1970) determined the
    dietary concentrations of Aroclors 1232 to 1262, administered over a
    5-day period, that were required to kill 50% of groups of mallards,
    pheasants, and quails. The concentrations were in the range of 500 to
    over 5000 mg/kg, with the bobwhite quail the most sensitive and the
    Japanese quail the least. There was a positive relationship between
    the percentage of chlorine in a technical Aroclor and its toxicity.
    The relationship held true for those containing less than 60%
    chlorine, Aroclor 1260 and 1262 deviating slightly. Mallards were
    relatively less responsive to chlorine content than the three
    gallimaceous species. Prestt et al. (1970) found a 50% mortality in
    Bengalese finches receiving a daily oral dose of Aroclor 1254 at
    254 mg/kg body weight for 56 days; cormorants have been killed with a
    cumulative dose of 5.7 g of Clophen A60, but herons were more
    resistant (Koeman et al., 1973).

        The toxicity to chickens of diets containing Phenochlor DP6,
    Clophen A60, or Aroclor 1260 at 400 mg/kg has been studied by Vos &
    Koeman (1970). The first two produced a 100% mortality with survival
    times of 24.3 and 20.5 days, but there was only 15%o mortality with
    the Aroclor over the 60-day test period. The authors demonstrated the
    presence of impurities in the Phenochlor and Clophen (Vos et al.,
    1970). The results of Flick et al. (1965) and Platonow & Funnel (1971)

    indicate that chickens can survive 200 mg/kg in the diet for several
    months, but that deaths may occur at 250 mg/kg, though Rehfeld et al.
    (1971) found a much higher toxicity, recording 1/30 deaths with
    Aroclor 1248 at 30 mg/kg of diet over 25 days and 16/30 at 50 mg/kg.
    Keplinger et al. (1971) found decreased growth in chickens receiving
    Aroclor 1242 at 10 mg/kg and Aroclor 1254 at 100 mg/kg of diet, but no
    effects with Aroclor 1260 at 100 mg/kg. Birds severely poisoned with
    PCBs show tremors, ataxia, and ruffling and loss of feathers. Oedema
    of the abdominal and peritoneal cavities has been a characteristic
    sign at autopsy in some experiments (Flick et al., 1965) and this was
    observed in a very large number of chickens killed in Japan by the
    contamination of their feed with Kanechlor KC400 (Kohanawa et al.,
    1969a, 1969b). Enlargement of the kidneys and sometimes of the liver
    has been reported; some authors have noted a reduction in the size of
    the spleen, comb, and testes, an enlargement of the adrenals and
    thyroid, and a pale pancreas. No pathological signs were observed by
    Dahlgren et al. (1972) that could account for the deaths of pheasants,
    which occurred with a cumulative intake of about 900 mg of Aroclor
    1254 (daily oral administration of 20 or 200 mg to 11-week-old hens);
    they found a mean concentration of 520 mg/kg in brain tissue at death
    and it is possible that effects in the central nervous system were a
    contributory cause.

    7.1.3  Aquatic organisms

    7.1.3.1  Fish

        Stalling & Mayer (1972) report an oral LD50 of more than 1.5 g/kg
    for rainbow trout with Aroclors 1242 and 1260. A 15 mg/kg oral dose to
    cod affected their ability to maintain an upright position in rotating
    water (Lindahl, unpublished report 1974).

        The assessment of toxicity to fish by adding PCBs to aquarium
    water is subject to considerable error on account of the different
    solubilities of the components. Zitko (1970) claims the aqueous
    solubility of Aroclor 1221 to be 3.8-5 mg/litre and that of Aroclor
    1254 to be 0.3-0.5 mg/litre. Stalling & Mayer (1972) report the
    96-hour LC50s on the cut-throat trout to be 1.17 mg/litre for Aroclor
    1221 and up to 60 mg/litre for Aroclor 1260, where the solubility is
    clearly exceeded. In the more prolonged experiments of these authors
    (Table 8), most of the concentrations are within the solubility limits
    and therefore more reliable. In more prolonged experiments, Hansen et
    al. (1971) found deaths in fish exposed for up to 45 days to Aroclor
    1254 at 5 µg/litre, but none at 1 µg/litre. These results show that
    the effect of PCBs is cumulative in fish and that the toxicity
    decreases with increasing chlorination.

        Table 8.  Intermittent-flow bioassays of Aroclors against three species of fisha
                                                                                             

                                   LC50 (µg/litre)
                                                                                             
                                   5         10        15        20        25        30
    Aroclor   Species              days      days      days      days      days      days
                                                                                             

    1254      Rainbow trout        156         8        --        --        --        --
    1260                            --       240        94        21        --        --
    DDT                              2.26      0.87      0.26     --        --        --
    1242      Bluegills            154        72        54        --        --        --
    248                            307       160        76        10        --        --
    1254                            --       443       204       135        54        --
    1260                            --        --        --       245       212       151
    1242      Channel catfish       --       174       107        --        --        --
    1248                            --       225       127        --        --        --
    1254                            --        __       741       300       113        --
    1260                            --        --        --       296       166       137
    1248b     Bluegills            137        76        --        --        --        --
    1248c     Channel catfish       --        94        57        --        --        --
                                                                                             

    a  From Stalling & Mayer (1972).
    b  Temperature, 20°C; alkalinity 260 mg/litre pH 7.4.
    c  Temperature, 27°C.

    
        Young fish appear to be more sensitive to PCBs than adults,
    96-hour LC50s for newly hatched fathead minnows were 15 and
    8 µg/litre, respectively for Aroclors 1242 and 1254. Growth of young
    fathead minnows and flagfish was affected above 2.2 µg/litre (Nebeker
    et al., 1974). There is no information on pathological changes in fish
    that might be related to the lethal action of PCBs; Hansen et al.
    (1971) found that fish survived exposure to Aroclor 1254 at
    5 µg/litre, but died later, after being returned to clean water, with
    signs of a lowered resistance to infection. Pathological changes were
    observed in kidney, spleen, and liver of rainbow trout fed Aroclor
    1254 at 10 or 100 mg/kg of diet for up to 330 days (Nestel & Budd,
    1975).

    7.1.3.2  Aquatic invertebrates

        The results of several investigations into the toxicity of PCBs to
    aquatic invertebrates have been reported by Stalling & Mayer (1972).
    The exposure of a variety of scud  (Gammarus pseudolimnaeus) to PCBs
    showed a decreased toxicity with increasing chlorination; 4-day LC50s
    of 10, 52, and 2400 µg/litre were obtained with Aroclors 1242, 1248
    and 1254, respectively. The threshold for survival, growth, and
    reproduction of  Gammarus pseudolimnaeus exposed to Aroclor 1248 was
    about 5 µg/litre and was the same for  Daphnia magna. With the
    crayfish, 7-day LC50s were 30 µg/litre (Aroclor 1242) and 80 µg/litre
    (Aroclor 1254) and with the glass shrimp, 3 µg/litre (Aroclor 1254).
    Wildish (1970) found that mortality in  Gammarus oceanicus was
    dependent on the duration of exposure to Aroclor 1254 at levels above
    10 µg/litre. Stalling & Mayer (1972) reported the 15-day LC50 of
    Aroclor 1254 for immature pink shrimp to be 0.94 µg/litre.

    7.1.3.3  Microorganisms

        The growth of certain marine diatoms is inhibited by Aroclor 1254
    at 10-25 µg/litre, but marine and freshwater algae are more resistant,
    being unaffected by 100 µg/litre (Mosser et al., 1972). Fisher et al.
    (1972) found that phytoplankton from the Sargasso Sea did not grow in
    Aroclor 1254 at 10 µg/litre, though phytoplankton from estuarine and
    coastal waters were not much affected by this concentration. Keil et
    al. (1971) report that the growth of a diatom was inhibited by Aroclor
    1242 at 100 µg/litre with a reduction of RNA synthesis, but that
    10 µg/litre had no effect.

        The growth of cultures of lake bacteria was not inhibited by
    concentrations of Aroclors 1221, 1242, and 1254 in excess of
    solubility, and Aroclors 1221 and 1242 could be utilized as the sole
    source of carbon and energy (Wong & Kaiser, 1975).

    7.2  Toxicity of Impurities in Commercial PCBs

        Vos & Koeman (1970) observed that Phenochlor DP6 and Clophen A60
    were more toxic to chickens than was Aroclor 1260 (see section 7.1.2)
    and Vos & Beems (1971) showed a similar difference in the dermal
    toxicity to the rabbit (see section 7.1.1.4). Vos et al. (1970)
    subdivided Clophen A60 and Phenochlor DP6 into non-polar PCBs and
    polar fractions and found polar components that were not detectable in
    a similar fraction from Aroclor 1260. Phenoclor DP6, Clophen A60, and
    the polar fraction from Clophen A60 produced a high mortality in the
    chick embryo test but the polar fraction from Aroclor 1260 did not. A
    difference between the three fractions was seen in the development of

    skin lesions in the rabbit (Vos & Beems, 1971). Mass spectrographic
    analysis indicated that the impurities were tetra- and pentachloro-
    dibenzofurans. Additional contaminants were chlorinated naphthalenes.
    Vos et al. (1970) calculated that the maximum level of chlorinated
    dibenzofurans in Clophen A60 was 5 mg/kg and in Phenochlor DP6,
    20 mg/kg. They calculated that the chlorinated dibenzofurans were
    approximately one order of magnitude less toxic than the chlorinated
    benzodioxins, and considered that they were mainly responsible for the
    toxicity of the polar fraction and for the difference in toxicity
    between the three commercial PCB mixtures.

        Recently, chlorinated dibenzofurans were detected in the
    PCB-contaminated oil that was responsible for the Yusho disease in
    Japan (see section 8, p. 65).

        In 1961, Bauer et al. demonstrated the toxicity of a mixture of
    tri- and tetrachlorodibenzofurans; a single oral dose of 0.5-1.0 mg/kg
    body weight caused severe and often lethal liver necrosis in rabbits.
    Application to the rabbit ear resulted in hyperplasia and
    hyperkeratosis. Similar toxic effects were found with 2,3,7,8-tetra-
    chlorodibenzo- p-dioxin in doses that were 10 times lower than those
    found to be toxic in the case of chlorinated dibenzofurans. See also
    the review by Kimbrough (1974). 2,3,7,8-Tetrachlorodibenzofuran, which
    has recently been shown to be present in PCBs (Bowes et al., 1975)
    caused mortality in chickens after 8-15 days when they were dosed
    orally with 5 µg/kg/day. A single oral dose of 4000 µg/kg was not
    lethal to mice. Porphyria was not observed in chicks or mice
    (Goldstein et al., 1975a). In comparison, 8 out of 10 chicks died
    after 9-19 days when dosed orally with 2,3,7,8-tetrachloro-
    dibenzo- p-dioxin at 1 µg/kg body weight/day (Schwetz et al., 1973).
    The oral LD50 of 2,3,7,8-tetrachlorodibenzofuran in guinea pigs is
    approximately 7 µg/kg body weight, which is less than one order of
    magnitude higher than that of 2,3,7,8-tetrachlorodibenzo- p-dioxin
    (Moore, 1975).

        Using low resolution mass spectrometry, McKinney (1975) found
    metabolites in the excreta of chickens fed 2,4,6,2',4',6'-hexa-
    chlorobiphenyl which had correct masses for chlorodibenzofurans. In
    addition, by perchlorination, he identified octachlorodibenzofuran
    again by low resolution mass spectrometry. The 2,4,6,2',4',6'-hexa-
    chlorobiphenyl isomer is not found in commercial mixtures.

        Zitko et al. (1972b) looked for the presence of chlorinated
    dibenzofurans in fish and fish products taken from a contaminated
    coastal area. The samples included shark tissues, cormorant and
    herring gull eggs, herring oil, and herring fishmeal. The detection

    limit of the method was 0.01-0.02 mg/kg in the sample. No chlorinated
    dibenzofurans were detected, although the authors admit that the limit
    of detection was not sufficiently low to detect amounts that might
    exert a significant toxic action. Curley et al. (1975) detected a
    component which had a 4 Cl isotopic cluster and a mass number of 304
    corresponding with that of tetrachlorodibenzofuran in the urine of
    rats dosed with PCBs, but a positive identification was not made.

    7.3  Toxicity of the PCTs

        There have not been any systematic studies on the toxicity of the
    PCTs. Sosa-Lucero et al. (1973) administered diets containing Aroclor
    5460 at 0, 10, 100, and 1000 mg/kg to groups of rats for seven days.
    There were no adverse effects on health or body weight; a significant
    liver enlargement was recorded at the 1000 mg/kg level. In a test for
    estrogenic activity involving the stimulation of glycogen response in
    the immature rat uterus, Aroclor 5460 was inactive, as was Aroclor
    1260 but Aroclor 5442 was more active than Aroclor 1242 (Bitman et
    al., 1972). A dietary level of Aroclor 5460 of 5000 mg/kg for 12 weeks
    caused decreased body weight and increased liver weight in rhesus
    monkeys (Allen & Norback, 1973); after 6 weeks, facial oedema, hair
    loss, and eye discharge were observed as described by the same authors
    in experiments with Aroclor 1248 (section 7.1.1.2), and similar
    gastric changes were also reported.

    7.4  Biochemical Effects

    7.4.1  Induction of enzymes

        Several investigators have observed an increase in the smooth
    endoplasmic reticulum of liver cells after administration of PCBs (see
    section 7.1.1.3). This is accompanied by an induction of microsomal
    mixed function oxidase. Induction of microsomal enzyme activity is,
    like liver enlargement, more marked with the higher chlorinated PCBs
    and relatively low with Aroclors 1221 and 1016 (Villeneuve et al.,
    1971a, 1972; Bickers et al., 1972; Ecobichon & Corneau, 1974). The
    effect has also been demonstrated with single pure PCBs administered
    orally (Fujita et al., 1971); more recently this work has been
    confirmed by Johnstone et al. (1974) whose results are summarized in
    Table 9 and show a greater degree of enzyme induction with the higher
    chlorinated compounds.

        Grant et al. (1974) found an increase in microsomal enzyme
    activity in rats receiving Aroclor 1254 at 20 mg/kg of diet for 246
    days, but none at 2 mg/kg. Iverson et al. (1975) and Goldstein et al.
    (1975) compared the microsomal enzyme inducing potential of Aroclors
    1242 and 1016. Iverson et al. (1975) found increased hepatic
    microsomal enzyme activity with both Aroclors in male rats receiving

    21 daily oral doses of 1 mg/kg body weight, and with Aroclor 1242 in
    females at 10 mg/kg of body weight and with Aroclor 1016 at 100 mg/kg.
    PCBs administered to rats during pregnancy can induce microsomal
    enzyme activity in the placenta and fetus and this also occurs in the
    liver of newborn rats suckled by mothers fed with diets containing
    PCBs (Alvares & Kappas, 1975). Benthe et al. (1972b) reported that
    when rats were stressed by food deprivation or cold, the PCB residues
    in adipose tissue were released during mobilization of the fat and
    caused increased hepatic microsomal enzyme activity.

        Table 9.  Stimulation of microsomal enzyme activity by single chlorinated biphenyls
                                                                                             

                          Hepatic microsomal enzyme activity
                                                                                             
    Chlorine                                                  aniline
    substituents          O-demethylation   N-demethylation   hydroxylation    nitro-reduction
                                                                                             

    4                     0                 0                 0                0
    2,2'                  0                 +                 +                0
    2,4'                  0                 0                 +                0
    4,4'                  + +               + +               + +              +
    2,5,2',5'             0                 +                 + +              +
    2,4,2',4'             + +               + +               + +              0
    2,4,5,2',4',5'        + +               + +               + +              + +
    2,3,5,2',3',5'        + +               + +               +                + +
    2,4,6,2',4',6'        + +               + +               + +              + +
    2,3,4,5,2',3',4',5'   + +               + +               + +              + +
                                                                                             

    a  Johnstone et al. (1974).
       0    no activity.
       +    slight activity.
       + +  marked activity.

    
    7.4.2  Porphyria

        Hepatic porphyria has been induced by a number of PCBs (Clophen
    A60, Phenoclor DP6, Aroclors 1016, 1242, 1254, and 1260) in the
    chicken, rabbit, Japanese quail, and the rat (Vos & Koeman 1970; Vos &
    Beems, 1971; Iverson et al., 1975; Vos et al., 1971; Goldstein et al.,
    1974, 1975a, 1975b). Porphyrin induction has been studied more
    extensively in rats. A dose-dependent increase in liver porphyrins has
    been observed in females receiving 21 daily oral doses of Aroclor 1242
    at 10 and 100 mg/kg of body weight, but not at 1 mg/kg. Female rats

    were more sensitive than males, and Aroclor 1016 had less effect
    (Iverson et al., 1975). In rats receiving Aroclor 1254 at 100 mg/kg in
    the diet, the increase was usually delayed 2-4 months after the start
    of dosing (Goldstein, 1974) and was characterized by high hepatic and
    urinary levels of uroporphyrin. Disturbance of porphyrin biosynthesis
    had been connected with an increase of the rate limiting enzyme
    2.3.1.37-delta-aminolaevulinate synthase (Vos et al., 1971; Goldstein
    1974, 1975).

        Kawanishi et al. (1973, 1974) have shown that the administration,
    in the diet, of Kanechlors KC-300 and KC-500 to rats at 500 mg/kg
    produced a marked increase in urinary excretion of copro- and
    uroporphyrins, and in faecal excretion of protoporphyrin, but no
    increase was observed with KC-400. Experimental administration of pure
    tetrachlorobiphenyls did not produce porphyria. However, porphyria did
    result from the repeated subcutaneous injection of KC400 (total dose
    1.8 g) to rabbits for 55 days (Miura et al., 1973).

    7.4.3  Effects on steroid metabolism

        PCBs have been shown to stimulate the activity of enzymes
    responsible for metabolizing steroids such as estrodiol and
    androsterone more effectively than does DDT or DDE (Risebrough et al.,
    1968; Lincer & Peakall, 1970). It has been suggested that effects on
    reproduction (see section 7.4) may be attributed to the induction of
    steroid-metabolizing enzymes (Kihlström et al., 1973). Long-term
    administration of daily oral doses of 0.025 mg Clophen A60 to 23
    female NMRI strain mice caused a lengthening of the estrous cycle and
    a reduction in the frequency of implantation of ova Orberg &
    Kihlström, 1973).

    7.4.4  Other biochemical effects

        Hepatic vitamin A has been reported by Villeneuve et al. (1971a)
    to be reduced to half the normal values in pregnant rabbits by Aroclor
    1254. Similar observations have been made by Cecil et al. (1973) in
    male and female Japanese quails and in rats after feeding Aroclor 1242
    at 100 mg/kg of diet. The 50% decrease in hepatic vitamin A found in
    male and female rats was also found in male and female quail provided
    that the latter were kept in the dark to prevent egg laying.

        A lowering of hepatic vitamin A in rats, fed a 0.1% PCB diet (PCB
    not specified), was described by Innami et al. (1974). The rats given
    vitamin A supplement (3400 IU) plus PCB for 6 weeks showed better
    growth than those given PCB alone, while those given PCB and a vitamin
    A deficient diet showed significant growth retardation. On the PCB

    diet, the hepatic vitamin A level decreased to 20% of the normal level
    during the experimental period; supplementing the diet with 1000 IU
    did not re-establish the vitamin A level in the liver. Administration
    of 3000 IU of vitamin A with the PCB diet, however, allowed better
    than normal hepatic vitamin A levels to be re-established. The authors
    concluded that vitamin A may play a role in the detoxification of PCB
    rather than that PCB plays a role in the destructive metabolism of
    vitamin A.

        Aroclor 1254 administered intraperitoneally to rats at 25 mg/kg
    body weight daily for 4 days caused a 4- to 5-fold increase in the
    biliary excretion of thyroxine during a 3-hour period. Biliary
    clearance was greatly elevated. Hypobilirubinaemia has been produced
    in rats by Bastomsky et al. (1975) who investigated the mechanism by
    administering daily intra-peritoneal injections of Aroclor 1254
    (25 mg/kg body weight in corn oil) to female rats for 4 days, then
    measuring bilirubin glucuronide formation by hepatic microsomes
     in vitro. PCB treatment was not effective in increasing
    UDPglucuronosyltransferase (2.4.1.7) activity. Serum bilirubin levels
    were also significantly decreased by PCB treatment of Gunn rats, which
    are genetically deficient in UDPglucuronosyltransferase (2.4.1.7)
    activity.

    7.4.5  Potentiation and antagonism by PCBs

        As PCBs can stimulate microsomal enzyme activity, it is to be
    expected that they may potentiate the action of those chemicals that
    undergo microsomal activation, and decrease the action of those that
    are detoxified. Villeneuve et al. (1973) demonstrated the antagonistic
    effect by the reduction of phenobarbital sleeping time in rats
    receiving Aroclors 1242, 1254, and 1260 in their diet, but not in
    those receiving Aroclor 1221. Johnstone et al. (1974) have confirmed
    this with a series of single PCBs. Tanaka & Komatsu (1972) found that
    the hexobarbital-induced sleeping time in female rats was reduced to
    49% of the control value by daily oral doses of Kanechlor 500 of
    2 mg/kg for 3 days (total 6 mg/kg). When a daily dose of 0.4 mg/kg was
    given for 15 days (total 6 mg/kg), no reduction in sleeping time was
    observed. When this small dose was continued for 45 and 53 days, the
    reduction remained at 12-13%. Phillips et al. (1972) did not find any
    potentiation of the cholinesterase-inhibitory action of parathion in
    rats dosed with Aroclors 1221 and 1254; this does not necessarily
    imply that there was no enhanced activation of parathion, as a
    stimulation of detoxication may have occurred concurrently. A
    stimulation of parathion detoxication but not of activation has been
    demonstrated in rabbit microsomes (Villeneuve et al., 1971a).
    Lichtenstein (1972) reports a potentiation by PCBs of the toxicity of
    parathion to flies.

        Cecil et al. (1975) have shown that the ability of PCTs to
    decrease phenobarbital sleeping time in quails is rather less than
    that of the PCBs.

        Aroclor 1254 at 160 mg/kg of diet fed to 5-week old male and
    female Fischer-344 rats for 8 weeks reduced mortality due to feeding
    hexachlorophene at a concentration of 600 mg/kg of diet from 77% to 7%
    and completely prevented the paralysis that was observed in all
    animals on the hexachlorophene diet alone. However, in the animals on
    the combined treatment, histological changes in the brain
    characteristic of hexachlorophene were still apparent and the
    possibility of delayed toxicity beyond the 8 weeks of the experiment
    could not be eliminated. The protective effect of Aroclor 1254 was
    explained by its capacity to enhance detoxification by means of
    hepatic microsomal enzyme induction (Jones et al., 1974).

    7.5  Cytotoxic Effects

        Peakall et al. (1972) found a significant increase in chromosome
    abnormalities in the embryos of ring doves when the parents were fed
    on a diet containing Aroclor 1254 at 10 mg/kg. Nilsson & Ramel (1974)
    found no chromosome breakage in  Drosophila melanogaster when either
    Clophen A30 or A50 was added to the substrate. Green et al. (1975)
    administered Aroclor 1242 orally to rats in single doses of 1250,
    2500, or 5000 mg/kg or as a repeated dose of 500 mg/kg/day for 4 days.
    Aroclor 1254 was also administered for 5 days at doses of 75, 150, or
    300 mg/kg/day; they found no evidence of a mutagenic potential as
    assessed by cytogenic analysis of bone marrow and spermatogonia.

    7.6  Immunosuppressive Effects

        Administration of PCBs leads to an atrophy of lymphoid tissue in
    chickens (Flick et al., 1965; Vos & Koeman, 1970), in pheasants
    (Dahlgren et al., 1972), and in rabbits (Vos & Beems, 1971). Vos & de
    Roij (1972) and Vos & van Driel-Grootenhuis (1972) came to the
    conclusion that these effects could be attributed to an
    immunosuppressive effect of PCBs. They found that when guinea pigs fed
    on diets containing Clophen A60 or Aroclor 1260 at 50 mg/kg were
    stimulated with tetanus toxoid, a lower antitoxin titre and a lower
    count of antitoxin-producing cells was obtained than in control guinea
    pigs, resulting in a significant reduction of immunoglobulins. The
    skin reaction after tuberculination in animals immunized with Freund's
    complete adjuvant (as a parameter of cell-mediated immunity) was also
    depressed at the 50 mg/kg of diet level. Vos & de Roij (1972)
    suggested that the ability of PCBs to increase the susceptibility of
    ducklings to duck hepatitis virus (Friend & Trainer, 1970) and of fish
    to fungal disease (Hansen et al., 1971) could be attributed to this
    immunosuppressive effect. Kimuru & Baba (1973) found an increased
    incidence of pneumonia, and lung and intracranial abscesses in rats on
    a diet containing Kanechlor 400 and suggested that this was due to a
    lowered resistance to infection.

    7.7  Effects on Reproduction

        In a one-generation reproduction study, rats were fed with a diet
    containing Aroclor 1242, 1254, or 1260 at levels of 1, 10, and
    100 mg/kg. Decreased survival of pups with Aroclors 1242 and 1254 at
    100 mg/kg was noted. No effect on reproduction was detected with
    Aroclor 1260 (Keplinger et al., 1971). In a two-generation
    reproduction study (Linder et al., 1974), rats were fed a diet
    containing Aroclor 1254 at levels of 0, 1, 5, 20, and 100 mg/kg and
    Aroclor 1260 at levels of 0, 5, 20, and 100 mg/kg. Rats exposed to
    Aroclor 1254 at dietary levels of 20 mg/kg or more had fewer pups per
    litter. Aroclor 1260 had no effect on reproduction, even at levels of
    100 mg/kg. Kihlström et al. (1973) showed that a daily oral dose of
    25 µg of Clophen A60 to female mice for 62 days significantly
    increased the length of the estrus cycle and decreased the frequency
    of implanted ova. In order to study the effect of PCBs on the
    development of sexual functions in the early postnatal period they
    also mated mice that had been suckled by mothers dosed with Clophen
    A60 during the lactation period. A decrease in the frequency of
    implanted ova was noted when both parents of the couple had been
    suckled with milk containing PCBs.

        In the rhesus monkey, Allen (1975) reports a lower fertility and a
    diminished weight of the young at birth, after the administration of a
    diet containing Aroclor 1248 at 2.5 mg/kg for several months.

        Studies by Aulerich et al. (1971), and Ringer et al. (1972) showed
    that Aroclor 1254 fed to mink at 5 mg/kg severely affected
    reproduction.

        There have been several reports that PCBs can adversely affect egg
    production and hatchability in birds. Contamination of feed by PCBs
    from a heat exchanger was shown to be the cause of reduced
    hatchability of hen eggs at a large poultry hatchery in the USA
    (Kolbye, 1972), and this has been confirmed in laboratory experiments.
    Keplinger et al. (1971) found poor hatchability of eggs from hens
    receiving diets containing Aroclor 1242 at 10 mg/kg or Aroclor 1254 at
    100 mg/kg, but not with hens receiving Aroclor 1260 at 100 mg/kg. An
    adverse effect on eggs has been noted with Aroclor 1248 at 10 mg/kg
    (Panel on Hazardous Trace Substances, 1972). Peakall et al. (1972) fed
    ring doves on a diet containing Aroclor 1254 at 10 mg/kg for 3 months
    and found a marked reduction in egg hatchability 6 months later, due
    to embryo mortality.

        The viability of salmon eggs bears some relation to their PCB
    content; Johansson et al. (1970) found a 12% mortality in eggs
    containing PCBs at the rate of 9.2 mg/kg in extractable fat, and a
    100% mortality with 34 mg/kg. Adverse effects on the reproduction of
    aquatic invertebrates have occurred at water concentrations of PCBs in
    the region of 5 µg/litre.

        The potential teratogenic effect of the PCBs has been studied by
    dosing pregnant females during the gestation period. No fetal
    abnormalities were produced in the rat by daily doses of Aroclors
    1242, 1254, or 1260 at 10 and 30 mg/kg (Keplinger et al., 1971), or
    Aroclor 1254 at 100 mg/kg (Villeneuve et al., 1971b), or in the rabbit
    dosed with Aroclor 1254 at 10 and 50 mg/kg (Villeneuve et al., 1971b).
    Injection of PCBs into eggs has been reported to produce beak
    abnormalities in chicks (McLaughlin et al., 1963). Cecil et al. (1974)
    have claimed that the administration of Aroclors 1232 and 1254 to hens
    at 20 mg/kg in the diet causes teratogenic effects and a reduction in
    hatchability of fertile eggs.

    7.8  Neoplasia and Adenofibrosis

        The liver is the only organ where tumours have been reported
    following the ingestion of PCBs. Ito et al. (1973) fed groups of 12
    male dd mice with diets containing 500, 250, 100 and 0 mg/kg of
    Kanechlor 500, 400, and 300, respectively. After 1 year, 7/12 mice
    developed neoplastic nodules (hyperplastic nodules) and 5/12 developed
    hepatocellular carcinomas, all in mice from the group fed with
    500 mg/kg Kanechlor 500. Metastases were not observed. In a second
    study on mice, combined exposure to Kanechlor 500 and either alpha- or
    ß-hexachlorocyclohexane isomers enhanced the development of neoplastic
    nodules (hyperplastic nodules) and hepato-cellular carcinomas. A
    combination of Kanechlor 500 and gamma-hexachlorocyclohexane did not
    produce tumours. Dosing with Kanechlor 500 alone at a dietary level of
    250 mg/kg, and ß- or gamma-hexachlorocyclohexane at dietary levels of
    250, 100 or 50 mg/kg did not produce tumours. However, alpha-hexa-
    chlorocyclohexane produced 8/30 hepata-cellular carcinomas and 23/30
    hyperplastic nodules.

        Groups of 50 BALB/cj male inbred mice were fed dietary
    concentrations of Aroclor 1254 of 0 or 300 mg/kg (49.8 mg/kg body
    weight) for 6 or 11 months respectively. No liver tumours were noted
    in a total of 58 surviving controls. A total of 10 hepatomas
    (neoplastic or hyperplastic nodules) were noted in 9/22 surviving mice
    fed Aroclor 1254 for 11 months and in 1/24 surviving mice fed Aroclor
    1254 for 6 months. In addition adenofibrosis (cholangiofibrosis) was
    observed in all 22 livers of mice fed Aroclor 1254 for 11 months, but
    not in the other groups (Kimbrough & Linder 1974).

        In another study (Kimbrough et al., 1975), 200 female Sherman
    strain COBS random bred rats (descendants of the Osborne Mendel
    strain) were given a diet containing Aroclor 1260 at 100 mg/kg
    (11.6-4.3 mg/kg body weight) for approximately 21 months; 200 female
    rats were kept as controls. The rats were sacrificed when 23 months
    old. Hepatocellular carcinomas were found in 26/184 of the
    experimental groups and in 1/173 of the control rats. None of the

    controls, but 144/184 experimental rats had neoplastic nodules
    (hyperplastic nodules). Areas of hepatocellular alteration were noted
    in 28/173 controls and 182/184 experimental rats. No effect of the
    Aroclor on the incidence of tumours in other organs and no metastases
    from the liver tumours were observed. In this and two earlier studies,
    adenofibrosis of the liver was observed in male and female rats fed
    either Aroclor 1260 or Aroclor 1254 (Kimbrough et al., 1972; 1973);
    1975). Adenofibrosis of the liver is a persistent progressive lesion
    that consists of a marked proliferation of fibrous tissue and
    epithelial glandular cells which are well differentiated in the mouse
    but appear atypical in the rat.

        In a preliminary study, liver tumours (multiple adenomatous
    nodules) were induced by Kanechlor 400 in 6/10 female, but not in male
    Donryu rats. The dietary exposure varied throughout the study and the
    number of animals used was small (10 experimental and 5 control rats
    for each sex) (Kimura & Baba, 1973). Makiura et al. (1974) reported
    that PCBs (Kanechlor 500) inhibited the induction of liver tumours in
    male Sprague-Dawley rats by the known carcinogens 3' methyl- p-
    dimethylaminoazo-benzene, N-2-fluosenyl acetamide, and diethyl-
    nitrosamine.

    8.  EFFECTS OF PCBs AND PCTs ON MAN -- EPIDEMIOLOGICAL AND CLINICAL
        STUDIES

        In June 1968, patients appeared at the Dermatology Clinic of
    Kyushu University Hospital, Fukuoka, Japan suffering from chloracne. A
    group at the University undertook intensive clinical, chemical, and
    epidemiological investigations and found that the disease originated
    from the consumption of a batch of rice oil supplied in February 1968;
    the disease was called Yusho (rice oil disease) (Katsuki, 1969). This
    batch of rice oil was found to be contaminated with Kanechlor 400, a
    48% chlorinated biphenyl, at 2000-3000 mg/kg which entered the oil
    through a leak in a heat exchanger (Tsukamoto et al., 1969). The
    symptoms and signs of Yusho were described by Goto & Higuchi (1969)
    and by Okumura & Katsuki (1969). The earliest signs were enlargement
    and hypersecretion of the Meibomian glands of the eyes, swelling of
    the eyelids, and pigmentation of the nails and mucous membranes,
    occasionally associated with fatigue, nausea, and vomiting. This was
    usually followed by hyperkeratosis and darkening of the skin with
    follicular enlargement and acneform eruptions, frequently with a
    secondary staphylococcal infection. These skin changes were most often
    seen on the neck and upper chest, but in severe cases extended to the
    whole body. Biopsy skin samples showed hyperkeratosis, dilation of the
    follicles, and an accumulation of melanin in the basal cells of the
    epidermis; melanin granules have also been observed in biopsy samples
    of the conjunctiva. Oedema of the arms and legs was seen in some
    patients. There were no definite signs of liver enlargement or liver
    disorders (Okumura & Katsuki 1969), but slight rises in serum
    transaminases and in alkaline phosphatase were detected, and a liver
    sample from a Yusho patient showed an increase in the smooth
    endoplasmic reticulum (Hirayama et al., 1969). The majority of the
    patients were found to have respiratory symptoms, and suffered from a
    chronic bronchitis-like disturbance that persisted for several years
    (Shigematsu et al., 1971, 1974).

        Yusho patients did not appear to suffer from central nervous
    effects, but some complained of numbness of the arms and legs. Mural &
    Kuroiwa (1971) found a decrease in the conduction velocity in
    peripheral sensory nerves.

        Yoshimura (1971) reported diminished growth in boys but not in
    girls, who consumed the oil. Babies born to Yusho mothers were smaller
    than normal. Newborn babies showed a dark brown skin pigmentation,
    which disappeared after a few months (Yagamuchi et al., 1971; Taki et
    al., 1969). Funatsu et al. (1972) found spotted and sporadic
    ossification of the skull and facial oedema with exophthalmia in four
    babies, but there was no evidence of any teratogenic action.

        Determinations of PCB concentrations in the tissue of Yusho
    patients were made several months after the ingestion of the oil,
    apparently by an X-ray fluorescence method for organic chlorine (Goto
    & Higuchi, 1969). Abdominal fat contained 13.1 mg/kg, subcutaneous fat
    75.5 mg/kg, and nails 59 mg/kg. The mesenteric adipose tissue in six
    Yusho patients, analysed by gas-liquid chromatography 1-3 years after
    the occurrence of intoxication, contained PCB levels of 2.5 mg/kg on
    average, which was considerably higher than the normal value. (Masuda
    et al., 1974a). The mean blood level of PCBs in patients was 0.6 or
    0.7 µg/100 ml (0.3 µg/100 ml for the general population) five years
    after exposure (Masuda et al., 1974b; Takamatsu et al., 1974). These
    authors also noted a specific gas-liquid chromatographic pattern,
    peculiar to Yusho patients, which is still observed.

        Hirayama et al. (1974) also reported that the serum bilirubin
    level of patients was significantly lower than the normal level and
    was negatively correlated with the blood level of PCBs and the serum
    triglyceride level.

        A considerable number of patients had elevated serum triglyceride
    levels, up to four times the normal values, although this was not
    correlated with the severity of the symptoms; these high values were
    maintained for three years in many patients (Uzawa, 1972). There were
    no marked abnormalities in serum cholesterol and phospholipid levels
    (Okumura & Katsuki, 1969; Uzawa et al., 1969). Nagai et al. (1969)
    reported an increase in urinary 17-ketosteroid excretion. Kusuda
    (1971) also observed changes in the menstrual cycle in approximately
    60% of 81 female Yusho patients as compared with their cycles prior to
    exposure. Okumura et al. (1974) examined the relationship between the
    blood levels of triglycerides and PCBs in 42 patients and observed a
    positive correlation. Uzawa et al. (1972) showed that high values of
    serum triglycerides were maintained for 3 years in many patients.
    Shigematsu et al. (1971) examined serum immunoglobulin levels in 38
    patients, 2 years after onset, and observed a decrease in IgA and IgM
    and an increase in IgG. Saito et al. (1972) reported lower IgM levels
    in patients showing chloracne.

        Urabe (1974) reported that the total number of Yusho patients had
    reached 1200 by 13 September 1973 and that 22 of them had died.
    Mucocutaneous signs had decreased year by year, but neurological and
    respiratory signs and symptoms and various complaints such as general
    fatigue, anorexia, abdominal pain, and headache had become more
    prominent among the patients. The smallest amount of oil that produced
    symptoms when ingested over approximately 120 days, contained
    approximately 0.5 g of PCBs, or approximately 0.07 mg/kg body weight
    per day (Kuratsune, 1972a). Recently chlorinated dibenzofurans at
    5 mg/kg were found in three samples of the toxic rice oil that
    contained PCB levels of about 1000 mg/kg (Nagayama et al., 1975).

        Symptoms similar to those of Yusho have been observed in workers
    in a Japanese condenser factory, including pigmentation of the fingers
    and nails, and acneiform eruptions on the jaw, back, and thighs. It
    was thought that these effects arose from local contact with PCBs;
    when the use of PCBs ceased, the symptoms disappeared (Hasegawa et
    al., 1972b).

    9. EVALUATION OF HEALTH RISKS TO MAN FROM EXPOSURE TO PCBs AND PCTs

    9.1  Species Variation

        The data in sections 7 and 8 indicate that man appears to be the
    species most sensitive to PCBs, the consumption of relatively small
    amounts having resulted in a severe disease (Yusho) in 1200 persons in
    Japan. The monkey is the only experimental species in which effects
    qualitatively and quantitatively approaching those in man have been
    observed; Allen (1975) attributed this to metabolic differences
    leading to a slower elimination than that observed in other species
    tested.

        Conclusions concerning the specific effects of PCBs on different
    species are confused by uncertainty arising from the presence of toxic
    impurities. The rice oil that caused the outbreak of Yusho was
    contaminated with PCBs containing relatively high amounts of
    tetrachlorodibenzofuran (see section 8), but the sample used in the
    monkey experiments had a low content of these impurities, so it is not
    clear whether PCBs alone were responsible for the Yusho incident.
    Further uncertainty arises from reports from Finland of high PCB
    concentrations in blood and body fat of occupationally exposed workers
    with no indication of adverse effects, while at similar tissue
    concentrations Japanese workers showed skin lesions typical of Yusho
    (see section 5.5.3).

        A species-specific toxic manifestation that can probably be
    attributed to toxic impurities, is the abdominal oedema and
    hydropericardium seen in birds affected by some commercial PCB
    mixtures.

        Mink is another species showing a high sensitivity to PCBs. Deaths
    have been produced with diets containing PCB levels of 30 mg/kg; no
    information is available on any species-specific metabolic pathway in
    the mink that would account for this susceptibility.

    9.2  Dose-Effect Relationships

        The following is a summary of the data in Sections 7 and 8
    concerning the relationship between mammalian toxicity and dose.
    Approximate calculations of the daily dose in mg/kg body weight
    derived from the dietary concentration are given in parentheses.a

              

    a  When no food consumption figures were available from the
       experimental studies, the following factors were used to transform
       mg/kg in the diet to mg/kg body weight: mouse (7), rat (20),
       guinea-pig (25), mink (10), rabbit (33), monkey (25).

    9.2.1  Body weight

        Body weight was reduced in rats after 8 months of dietary intake
    of Aroclor 1254 at 100 mg/kg (corresponding to 5 mg/kg body weight);
    no effects were observed at 20 mg/kg in the diet (corresponding to
    1 mg/kg body weight).

        Dose-dependent retardation of weight gain was observed in mink
    after 4 months of dietary intake of Aroclor 1254 at 5 and 10 mg/kg
    (corresponding to 0.5 and 1.1 mg/kg body weight respectively).

    9.2.2  Effects on liver

    Liver weight

        Dose-dependent increase in liver weight was observed in rats
    receiving Aroclors 1242, 1254 and 1260 at concentrations of more than
    20 mg/kg in the diet (corresponding to > 1.4 mg/kg body weight). Male
    rats were more sensitive than female rats; no effects were observed
    with Aroclors 1254 and 1260 at concentrations lower than 20 mg/kg in
    the diet (corresponding to < 1.4 mg/kg body weight). Effects were
    less marked with the lower chlorinated PCBs.

    Liver changes

        Smooth endoplasmic reticulum proliferation with fat droplet
    inclusions were observed in the liver tissue of rats after 8 months of
    dietary intake of Aroclor 1254 at 20 mg/kg (corresponding to 1.4 mg/kg
    body weight).

        Liver damage was observed with Aroclors 1242 and 1254 in rabbits
    receiving 14 weekly oral doses of 150 mg/kg body weight; no effect was
    observed with Aroclor 1221.

    Liver enzyme activitya

        Increase in microsomal enzyme activity was observed in male rats
    after 8 months of dietary intake of Aroclor 1254 of 20 mg/kg
    (corresponding to 1 mg/kg body weight). No effect was observed at
    2 mg/kg in the diet (corresponding to 0.1 mg/kg body weight. Effects
    were less marked in female rats.

        Increased activity was also observed with Aroclors 1242 and 1016
    in male rats receiving 21 daily oral doses of 1 mg/kg body weight.

                 

    a  According to Litterst, et al. (1972) the dose producing an effect
       on nitroreductase activity in the rat corresponds to 0.5 mg/kg in
       the diet (corresponding to 0.3 mg/kg body weight).

    Liver porphyria

        Effects were observed in rats after several months of dietary
    intake of Aroclor 1254 at 100 mg/kg (corresponding to 5 mg/kg body
    weight); dose-dependent effects were observed in female rats after 21
    daily oral doses of Aroclor 1242 at 10 and 100 mg/kg; no effects were
    noted at 1 mg/kg body weight.

    Liver vitamin A

        Reduction of hepatic vitamin A was observed in rats receiving
    Aroclor 1242 at the rate of 100 mg/kg in the diet (corresponding to
    5 mg/kg body weight).

    Liver tumours

        Hepatocellular carcinomas were observed in mice after one year of
    dietary intake of Kanechlor 500 at 500 mg/kg (corresponding to
    75 mg/kg body weight); no carcinomas were observed with Kanechlor 500
    at 250 mg/kg in the diet (corresponding to 37.5 mg/kg body weight), or
    with Kanechlor 300 and 400 at 500 mg/kg in the diet (corresponding to
    75 mg/kg body weight).

        Hepatomas were observed in mice after 10 months of daily intake of
    Aroclor 1254 at 300 mg/kg in the diet (corresponding to 49.8 mg/kg
    body weight).

        Hepatocellular carcinomas were observed in rats after 21 months of
    daily intake of Aroclor 1260 at 100 mg/kg in the diet (corresponding
    to 11.6-4.3 mg/kg body weight).

    9.2.3  Reproduction

        Effects on reproduction were observed in the mouse at a daily oral
    dose of 0.025 mg Clophen A60; in the rat at a dietary level of Aroclor
    1254 of 20 mg/kg (corresponding to 1 mg/kg body weight) with the
    effects decreasing with higher chlorinated PCBs; in the mink at a
    dietary level of Aroclor 1254 of 5 mg/kg (corresponding to 0.5 mg/kg
    body weight); and in the monkey at a dietary level of Aroclor 1248 of
    2.5 mg/kg (corresponding to 0.1 mg/kg body weight).

    9.2.4  Immunosuppression

        Immunosuppressive effects were observed in the guinea-pig at a
    dietary level of Clophen A60 or Aroclor 1260 of 50 mg/kg
    (corresponding to 2 mg/kg body weight).

    9.2.5  Skin effects

        In man, symptoms of Yusho disease were observed at a dietary level
    of 4.2 mg/day of PCBs (corresponding to 0.07 mg/kg body weight/day for
    a 60-kg person). A value of 0.50 g was estimated as the quantity of
    PCBs consumed over approximately 120 days above which toxic symptoms
    were evident. Similar effects were observed in the monkey at a dietary
    level of Aroclor 1248 of 2.5 mg/kg (corresponding to 0.1 mg/kg body
    weight) after several months.

    9.3  Nondetected effect levels

        The assessment of non detected effect levels for toxic effects is
    complicated by the different activities of the component PCBs and by
    the presence of impurities, in addition to the influence of inter- and
    intraspecies variation, age, sex, and length of exposure. Moreover,
    many of the available experimental studies do not include a
    nondetected effect level.

        The most sensitive species appears to be man, and effects have
    been observed at intake rates of 0.07 mg/kg body weight/day. This may
    have been influenced by the intake of impurities more toxic than PCBs,
    but similar effects have been produced in the monkey, at the same
    order of dosage, with a product containing little of these impurities.
    At this dosage level, no effects may be expected on growth, liver
    enlargement, and liver enzyme activity in less sensitive species such
    as the rat. Although non-detected effect levels are not available for
    effects on immunosuppression and reproduction, and for certain
    biochemical effects on the liver, it seems unlikely that these effects
    would be apparent at intake rates of 0.1 mg/kg body weight/day.
    Carcinogenic effects have been observed in rats and mice at doses two
    orders of magnitude greater than this, but there is no epidemiological
    evidence to suggest that PCBs cause tumours in man. According to Grant
    et al. (1974) rats fed on a diet containing Aroclor at the rate of
    2 mg/kg (equivalent to about 0.1 mg/kg body weight) showed PCB levels
    of 8 µg/100 ml in blood and 26.1 mg/kg in body fat. However, values
    much higher than these have been observed in men occupationally
    exposed to PCBs without evidence of any toxic effects (see section
    5.5.3).

        It is not possible at present to resolve this conflict in the
    evidence on the toxicity of PCBs to man.

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         Health Perspect., 1: 105-117.

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        polychlorinated biphenyls and fractions thereof in rabbits.
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        polychlorinated biphenyls in chickens with special reference to
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         Toxicol. appl. Pharmacol., 17: 656-668.

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        2,4,5,2',4',5'-hexachlorobiphenyl and a polychlorinated biphenyl
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        of the humoral and cell-mediated immunity in guinea pigs.  Sci.
         Total Environ., 1: 289-302.

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        M. G. & DE VOS, R. E. (1970) Identification and toxicological
        evaluation of chlorinated dibensofuran and chlorinated naphthalene
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    WESTOO, G. & NORÉN, K. (1972) Levels of organochlorine pesticides and
        polychlorinated biphenyls in Swedish human milk.  Var Föda,
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         Var Föda, 10: 341-360 (summary in English).

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        in sea water to  Grammarus oceanicus. Bull. environ. Contam.
         Toxicol., 5: 202-204.

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        plankton.  Mar. Bull., 4, 109-111.

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        the major components of a commercial polychlorinated biphenyl
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        polychlorinated biphenyls. II. Rate studies.  Bull. environ.
         Contam. Toxicol, 13: 249-256.

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        pregnant women having consumed rice oil contaminated with
        chlorobiphenyls and their babies.  Fukuoka Acta Med.,
        62: 112-117 (in Japanese).

    YAMAMOTO, H. & YOSHIMURA, H. (1973) Metabolic studies on
        polychlorinated biphenyls. III. Complete structure and acute
        toxicity of the metabolites of 2,4,3',4'-tetrachlorobiphenyl.
         Chem. pharm. Bull., 21 (10): 2237-2242 (in English).

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        tissue of the general population of the nation.  Environ. Health
         Perspect., 1: 79-81.

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        special reference to sex, age, clinical grades and oil
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        polychlorinated biphenyls. I. Metabolic fate of
        3,4,3',4'-tetrachlorobiphenyl in rats.  Chem. pharm. Bull.,
        21: 1168-1169 (in English).

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        polychlorinated biphenyls. IV. Biotransformation of
        3,4,3',4'-tetrachlorobiphenyl, one of the major components of
        Kanechlor-400.  Fukuoka Acta Med., 65: 5-11 (in Japanese).

    YOSHIMURA, H. & YAMAMOTO, H. (1975) A novel route of excretion of
        2,4,3',4'-tetrachlorobiphenyl in rats.  Bull. environ. Contam.
         Toxicol., 13: 681-687.

    YOSHIMURA, H., YAMAMOTO, H., NAGAI, J., YAE, Y., UZAWA, H., ITO, Y.,
        NOTOMI, A., MIMAKAMI, S., ITO, A., KATO, K. & TSUJI, H. (1971)
        Studies on the tissue distribution and the urinary and faecal
        excretion of 3H-Kanechlor (chlorobiphenyls) in rats.  Fukuoka
         Acta Med., 62: 12-19 (in Japanese).

    YOSHIMURA, H., YAMAMOTO, H. & SAEKI, S. (1973) Metabolic studies on
        polychlorinated biphenyls. II. Metabolic fate of
        2,4,3'4'-tetrachlorobiphenyl in rats.  Chem. pharm. Bull.,
        21: 2231-2236 (in English).

    YOSHIMURA, H., YAMAMOTO, H. & KINOSHITA, H. (1974) Metabolic studies
        on polychlorinated biphenyls. V. Biliary excretion of
        5-hydroxy-2,4,3',4',-tetrachlorobiphenyl, a major metabolite of
        2,4,3',4'-tetrachlorobiphenyl.  Fukuoka Acta Med., 65: 12-16
        (in Japanese).

    ZIMMERLI, B. & MARES, B. (1973) Die Belastung der Schweizerischen
        Bevolkerung mit Pestiziden.  Mitt. Lebensm. Unters. Hyg.,
        64: 459-479.

    ZITKO, V. (1970) Polychlorinated biphenyls (PCB) solubilized in water
        by nonionic surfactants for studies of toxicity to aquatic
        animals.  Bull. environ. Contam. Toxicol., 5: 279-285.

    ZITKO, V. (1971) Polychlorinated biphenyls and organochlorine
        pesticides in some fresh-water and marine fishes.  Bull. environ.
         Contam. Toxicol., 6: 464-470.

    ZITKO, V., HUTZINGER, O. & SAFE, S. (1971) Retention times and
        electron-capture detector responses of some individual
        chlorobiphenyls.  Bull. environ. Contam. Toxicol., 6: 160-163.

    ZITKO, V., HUTZINGER, O., JAMIESON, W. D. & CHOI, P. M. K. (1972a)
        Polychlorinated terphenyls in the environment.  Bull. environ.
         Contam. Toxicol., 7: 200-201.

    ZITKO, V., HUTZINGER, O. & CHOI, P.M. K. (1972b) Contamination of the
        Bay of Fundy-Gulf of Maine area with polychlorinated biphenyls,
        polychlorinated terphenyls, chlorinated dibenzodioxins and
        dibenzofurans.  Environ. Health Perspect., 1: 47-50.

    ZITKO, V., CHOI, P. M. K., WILDISH, D. J., MONAGHAN, C. F. & LISTER,
        N. A. (1974) Distribution of PCBs and  p,p'-DDE residues in
        Atlantic herring and yellow perch in eastern Canada, 1972.
         Post. Mon. J., 8: 105-109.

    


    See Also:
       Toxicological Abbreviations
       Polychlorinated biphenyls and terphenyls (EHC 140, 1992, 2nd edition)