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    DITHIANON

    First draft prepared by
    J.E.M. van Koten-Vermeulen and E.M. Den Tonkelaar
    National Institute of Public Health and Environmental Protection
    Bilthoven, Netherlands

    EXPLANATION

         Dithianon is used as a multi-site protective fungicide which
    inhibits spore germination. It is used on a range of fruits and
    vegetables. Dithianon was considered for the first time by the
    present Meeting.

    EVALUATION FOR ACCEPTABLE DAILY INTAKE

    BIOLOGICAL DATA

    Biochemical aspects

    Absorption, distribution, and excretion

    Rats

         Four male and 4 female rats were administered once daily 25 mg
    14C-dithianon (dioxonaphtyl-labelled)/kg bw/day in dimethyl
    sulfoxide for 7 days. Faeces and urine were collected at daily
    intervals until 8 days after the last administration. After 24 h,
    males eliminated 24.3% via urine and 37.1% via faeces and females
    22.9% and 32.2%, respectively. Seventy-two hours after the last
    treatment, 1.0% (males) and 1.8% (females) of the administered dose
    was still found in faeces and urine. Total recovery was 69.1% and
    68.0% in the faeces and 27.5% and 28.3% in urine of males and
    females, respectively. The carcass contained 0.1% of the total
    administered radioactivity (Schlüter, 1985).

         Radioactivity in blood was measured in 5 rats/sex over a period
    up to 6 days following a single oral dose of 25 mg 14C-
    dithianon/kg bw. Plasma radioactivity levels 15 minutes after dosing
    were 0.07 mg/l (males) and 0.15 mg/l (females) increasing to peak
    levels of 1.2 mg/l (males) and 1.9 mg/l (females) 8 h after dosing.
    Thereafter the values decreased rapidly to 0.07 mg/l after 144 h for
    both sexes (Schlüter, 1985).

         Rats (5/sex) were given a single oral dose of 25 mg 14C-
    dithianon/kg bw/day for 7 days. Tissue distribution was measured
    over 5 periods up to 7 days after the last treatment. Twenty-four
    hours after the last treatment, most tissue levels of radioactivity
    were below 0.01% of the administered dose except for liver (0.01-
    0.02%) and stomach and intestine (0.3-2.2%). After 7 days, all
    tissue residues were < 0.01% of the administered radioactivity
    (Schlüter, 1985).

         One rat/sex was given a single oral dose of 50 mg 14C-
    dithianon (dioxonaphtyl-labelled)/kg bw. After 5 days, a total of
    95.8% and 101.3% was excreted by the male and female rat,
    respectively. Urinary excretion was almost complete within 48 h and
    accounted for 33.5%-30.3% of the total radioactivity. Faecal
    excretion was complete by 72 h and accounted for 62.1% and 70.9%. No
    radioactivity was detected in the expired air during the 48 h after
    dosing (Hawkins  et al., 1989).

         Groups of rats (5/sex/group) were administered a single oral
    dose of 10 or 50 mg 14C-dithianon. Total recovery was about 97%
    regardless of the dose. After 5 days most of the radioactivity was

    excreted in the faeces (about 65%) while 31% was recovered in urine.
    Elimination occurred predominantly during the first 24 h. Dithianon
    was absorbed to approximately the same extent at the low- and high-
    dose levels. Only a small proportion of the administered dose was
    recovered in tissues. Highest amounts after the low-dose
    administration were in kidneys (males 0.16 mg/kg and females 0.17
    mg/kg), GIT (males and females 0.05 mg/kg) and whole-blood (males
    0.04 mg/kg and females 0.05 mg/kg). After the administration of 50
    mg the highest tissue residues were also found in kidneys (males
    0.58 mg/kg and females: 0.66 mg/kg), GIT (males 0.15 mg/kg and
    females 0.23 mg/kg) and whole-blood (males 0.28 mg/kg and females
    0.36 mg/kg) (Hawkins  et al., 1989).

         The repeated administration of 14 daily non-labelled doses of
    10 mg dithianon/kg bw/day followed by a single oral dose of 10 mg
    14C-dithianon/kg bw had no significant effect on the absorption,
    excretion and distribution of dithianon in rats (Hawkins  et al.,
    1989).

         Two groups of 3 rats/sex with cannulated bile ducts received a
    single oral dose of 10 or 50 mg 14C-dithianon/kg bw. Bile samples
    were collected at 3-h intervals and urine and faeces were collected
    at 24 h intervals. The rats were sacrificed 48 h after dosing. About
    10 and 7% of the dose was recovered in the bile at the low and the
    high dose, respectively (Hawkins, 1989).

         After a single oral administration of 10 or 50 mg 14C-
    dithianon/kg bw to rats radioactivity in plasma was measured at
    various time intervals up to 240 h after dosing. Peak plasma levels
    were reached in 6 h (males 0.99 mg/l and 3.9 mg/l and females 0.76
    mg/l and 3.81 mg/l at the low- and high-dose, respectively). At 240
    h all concentrations were below the detection limit. The terminal
    half-lives were similar for both dose levels, 55.8 and 46.4 h for
    males and 56.8 and 56.7 h for females, at 10 and 50 mg/kg bw,
    respectively (Hawkins  et al., 1989). 

    Hens

         Groups of 5 laying hens were given capsules containing 0, 0.36
    or 3.6 mg 14C-dithianon/day (purity 99.3%), orally for 5
    consecutive days. These doses were equivalent to 3 or 30 ppm in the
    feed, respectively. Excreta and eggs were collected twice daily.
    Radioactivity was excreted very rapidly and at a constant rate.
    Irrespective of the dose, 4 days after the last administration,
    total recovery of 14C was about 94% of the dose with about 90%
    recovered in the excreta and 3-5% in the GIT contents. Detectable
    tissue residues were found in kidney and liver (0.03% and 0.02%,
    respectively). The 14C recovered in eggs comprised less than 0.01%
    of the total radioactive dose. The radioactivity in egg yolk was
    higher than in egg white and was maximal at sacrifice (0.005 mg/kg

    and 0.075 mg/kg at the low- and the high-dose, respectively) (Cheng,
    1990a). 

    Goats

         Two lactating goats were given 14C-dithianon in capsules
    orally for 5 consecutive days at a dose level of 6 mg/day or 60
    mg/day. These dose levels were equivalent to 3 or 30 ppm in the
    feed, respectively. A control goat received placebo capsules. Body-
    weight, food consumption and clinical signs were recorded. Daily
    samples of urine, faeces and milk were collected. The goats were
    sacrificed 5 h after the last dose. Excretion was rapid and constant
    with the majority excreted in the faeces (50.2% to 53.7%) followed
    by the urine (27.9% and 24.2%). Less than 0.1% of the total
    radioactivity was recovered in the milk. Concentrations of
    radioactivity in milk, muscle and fat were less than 0.003 and 0.03
    mg/kg for the low- and high-dosed animal. The levels in liver (0.02
    and 0.17 mg/kg) and kidneys (0.06 and 0.49 mg/kg) were higher.
    Concentration in the bile was high, 0.3 and 2.9 mg/kg for the low-
    and the high-dose, respectively. This points to biliary involvement
    in the excretion also for the goat (Cheng, 1990b). 

    Biotransformation

         After 7 daily treatments of 14C-dithianon (dioxonaphtyl-
    labelled), urinary and faecal extracts were studied with thin-layer
    chromatography. There was no difference between males and females.
    The compound is quickly metabolized to a great number of polar
    compounds. None of these compounds exceeded 5% of the administered
    radioactivity. According to the authors this is due to the molecular
    structure of the substance, having different functional groups (CO,
    CN, S). Also cleavage of the molecule in various places is possible.
    It is assumed that very reactive products are formed which are
    immediately degraded further or react with endogeneous compounds in
    the animal to form polar products which are rapidly excreted. Less
    than 1% unchanged dithianon is found in the faeces (Schlüter, 1985).

         In the study of Hawkins  et al. (1989) extracts of urine and
    faeces were examined by TLC. More than 14 components were detected
    in the urine. The three major components had Rf values of 0.98, 0.55
    and 0.36. The two more polar compounds (Rf 0.55 and 0.36) were
    hydrolyzed with ß-glucuronidase or sulfatase giving rise to a higher
    proportion of the component with Rf value of 0.98. In the faeces the
    major components had the same chromatographic properties as those
    found in urine extracts. No attempt was made to characterize any of
    the metabolites.

    Toxicological studies

    Acute toxicity studies

         The acute toxicity of dithianon to several animal species is
    given in Table 1. Signs of toxicity following oral administration
    included sedation, dyspnoea, abnormal appearance, changes in gait
    and body posture, emaciation and diarrhoea. 

         The acute oral toxicity in Wistar rats of the formulation Delan
    Liquid (25% dithianon) was 1325 mg/kg bw (Sommer & Frohberg, 1968a).

    Short-term toxicity studies

    Mice

         In a range-finding study, groups of 6 Crl:CD-1(ICR)BR mice/sex
    were fed diets containing 0, 100, 500 or 1000 ppm dithianon (purity
    92%) for 4 weeks. Observation included clinical signs, body-weight,
    food consumption, haematology and clinical chemistry. Macroscopical
    examinations were performed on all mice, but weight and
    histopathology of liver and kidney were examined in female mice
    only. Body-weight gain was decreased at the highest dose. Hb, PCV
    and RBC counts were slightly lower in male mice at 1000 ppm and in
    female mice at 500 and 1000 ppm. T3 and T4 concentrations were
    lower in treated mice (dose-related in females). Increased relative
    liver and kidney weight were observed in mid- and high-dose females.
    A dose-related increased deposition of pigment in Kupffer cells was
    seen in 4/6 female livers at 500 ppm and in 6/6 female livers at
    1000 ppm (Brown, 1987). 

    Rats

         Groups of 10 rats/sex were fed diets containing 0, 30 or 180
    ppm dithianon (purity 92%) equal to 0, 2.5 and 14.6 mg/kg bw/day for
    males and 0, 2.97 and 16.3 mg/kg bw/day for females, respectively,
    for 90 days. The high-dose group received 1080 ppm (equal to 86.7
    mg/kg bw/day for males and 99.5 mg/kg bw/day for females) and
    consisted of 20 rats/sex of which 10/sex were kept after the 90-day
    dosing for a 4-week recovery period. Observations included clinical
    signs, body-weight, food consumption, haematology, clinical
    chemistry, urinalysis, ophthalmoscopy, auditory acuity and
    dentition, macroscopy, organ weight and histopathology. Body-weight
    gain was decreased in high-dose males and females; Hb and Ht and the
    number of erythrocytes was significantly decreased and the number of
    reticulocytes was increased. Male rats at all dose levels showed
    significantly decreased T3 levels (not clearly dose-related) and
    at the highest dose decreased T4 levels. TSH was slightly
    decreased in males at low- and mid-dose and increased at the highest

        Table 1. Acute toxicity of dithianon
                                                                                  
                                      LD50       LC50
    Species  Strain    Sex   Route    mg/kg bw   mg/m3   Reference
                                                                                  
    
    Mouse1   JCL.ICR   M     oral     492                Sakamoto  et al. (1975)
                       F              528
                       M     i.p      100
                       F              77
                       M&F   dermal   >3200
                       M&F   s.c.     >3200

    Mouse    NMRI      M&F   i.p.     49                 Sommer & Frohberg (1969)

    Rat2     KFM-Han   M     oral     720                Ullmann (1987a)
             Wistar    F              678

    Rat3     KFM-HAN   M&F   inhal.              2089    Ullmann (1984)
             wistar          (4-hr
                             exp.)

    Rat2     KFM-Han   M&F   dermal   >2000              Ullmann (1986a)
             wistar          (24-hr
                             exp.)
                                                                                  

    1    the purity of dithianon was 95%
    2    the purity of dithianon was 92%
    3    technical dithianon of 94.7% purity was used.
    
    
    dose. Blood urea was increased in high-dose males (statistically
    significant) and females. Chloride was decreased in females at 1080
    ppm as was total protein. Albumin and alpha1-globulin was
    increased in males (statistically significant) and females.
    Urinalysis showed that, in males at 1080 ppm, specific gravity was
    decreased. Absolute and relative liver and kidney weight and
    relative adrenal weight were slightly increased in both sexes at 108
    ppm. In females a tendency to an increased absolute and relative
    kidney weight was seen at 180 ppm. This is considered to be an
    effect because the kidney in the female rat is the target organ.
    After the recovery period, females still exhibited a slightly
    depressed erythrocyte count and an increased kidney weight and in
    males T4 levels were still depressed, all at the highest dose. No
    histopathological lesions were found. The NOAEL in this study was 30
    ppm, equal to 2.5 mg/kg bw/day, based on increased kidney weight in
    females (Leuschner & Neumann, 1987). 

         Kidney slides of 10 control female rats and of 20 rats at 1080
    ppm (including 10 from the recovery experiment) were re-evaluated.
    The incidence of hydropic degeneration and tubular hyperplasia was
    increased in treated rats at termination of the treatment period
    (Grasso, 1991a). 

         Groups of rats (5/sex/group) were exposed to 0.11, 0.31 or 1.07
    g dithianon/m3, 6 h/day, 5 days/week for 2 weeks (head/nose
    exposure). One of the mid-dose groups was kept for a recovery period
    of 14 days. Additionally, 2 groups of 5 rats/sex were exposed to
    filtered air and the vehicle alone, respectively. No dose-related
    effects were observed on clinical signs, body-weight, food
    consumption, haematology, blood chemistry, urinalysis, organ weight,
    macroscopy or histopathology. The NOAEL in this study was > 1.07
    g/m3 (Bhide, undated). 

         Groups of Wistar rats (9/sex/group) were exposed to an aerosol
    of 0 or 33.6 mg/m3 Delan WP (equal to 26 mg/m3 dithianon), 4
    h/day, 6 days/week for a total of 13 exposures (head/nose exposure).
    No effects were observed on clinical signs, body-weight, food
    consumption, haematology, clinical chemistry, macroscopy or
    histopathology. The NOAEL in this study was > 33.6 mg/m3 Delan
    WP, equal to 26 mg/m3 dithianon (Sommer & Frohberg, 1971; Spicer,
    1971a). 

         In a 5-day dermal range-finding study, 2000 mg dithianon/kg
    bw/day was administered to 5 Crl:CD(SD)BR rats/sex for 6 h/day on 5
    consecutive days. Brown staining was observed at the application
    site and on surrounding hair with dermal erythema or fissuring.
    Body-weight and food consumption were slightly reduced in treated
    male rats (Lackenby, 1987). 

         In a dermal limit test, suspensions of dithianon (purity 92%)
    in PEG 200 were applied to the skin of Crl:CD(SD)BR rats
    (5/sex/group) at doses of 0 or 1000 mg/kg bw/day, 6 h/day on 22
    consecutive days. Mortality, food consump-tion and blood
    biochemistry showed no dose-related effects. Body-weight gain was
    decreased in treated males. In treated females, Hb, RBC and PCV were
    slightly lower and MCV was slightly higher. Adrenal and kidney
    weights were increased in female rats. At histopathology slight
    irritation was observed in the treated skin. The incidence of
    basophilic tubules was increased in the kidneys of treated rats; in
    some of the females, the lesion was associated with the presence of
    occasional mitotic figures and eosinophilic material in the tubular
    lumen (Brown, 1989).

         Because of the effects seen at 1000 mg/kg bw/day, an additional
    study was carried out according to the same protocol with 5
    rats/sex/group administered 0, 40 or 200 mg dithianon/kg bw/day. At
    both dose levels the same haematological changes were observed not
    only in females but also in some treated males. Body weight was

    decreased and adrenal, kidney and liver weights were increased in
    treated rats. Skin damage (moderate to marked acanthosis, slight to
    moderate hyperkeratosis, leucocyte infiltration in the stratum
    corneum and the dermis and occasionally ulceration) was observed in
    all treated rats. Most of the systemic effects may be secondary, due
    to stress caused by the marked local irritation. A NOAEL was not
    determined in this study (Brown, 1989). 

    Dogs

         In a 4-week range-finding study, one beagle dog/sex was fed
    1000 ppm dithianon (purity 92%) for 14 days. After 7 days off-dose,
    they were treated for 7 days at 60 ppm. One dog/sex, used as control
    dog for the initial 15 days, was given 1000 ppm dithianon in the
    diet from days 15-21 and 1500 ppm from days 21-28. Food consumption
    and body-weight were markedly reduced at 1500 ppm, while at 1000 ppm
    only slight changes in food consumption were observed (Pickersgill,
    1987).

         Dithianon (purity 92%) was fed in the diet to groups of 4
    beagle dogs/sex at dose levels of 0, 40, 200 or 1000 ppm (equal to
    0, 0.6, 3.0 or 12.6 mg/kg bw/day for males and 0, 0.7, 3.0 or 12.6
    mg/kg bw/day for females, respectively) for 90 days. No dose-related
    effects were observed on mortality, clinical signs, haematology,
    urinalysis, eye examination, hearing, dentition, macroscopy or
    histopathology. Food consumption was decreased in females at 1000
    ppm. Body-weight gain was lower at the highest dose. A marked
    increase in ALP was observed in both sexes after 6 and 13 weeks at
    1000 ppm. The absolute and relative weights of liver, spleen and
    kidneys were increased in all high-dose dogs and absolute and
    relative thymus weight was decreased at the highest dose. No
    histopathological findings were observed. The NOAEL in this study
    was 200 ppm, equal to 3 mg/kg bw/day, based on increased organ
    weights at the high dose (Neumann & Leuschner, 1989). 

         Groups of beagle dogs (4/sex/group) were fed diets containing
    0, 40, 200 or 1000 ppm dithianon (purity 92%) for 52 weeks.
    Observations included clinical signs, food consumption,
    ophthalmoscopy, haematology, clinical chemistry, urinalysis,
    macroscopy, organ weight and histopathology. During the first 13
    weeks, food consumption was decreased in high-dose males. Male body-
    weight was lower during the whole study period. Hb, RBC and PCV were
    decreased in all high-dose dogs and MCHC was decreased in high-dose
    females throughout the study. At termination, platelet count was
    increased in high-dose males. In 3/4 high-dose males and 3/4 high-
    dose females erythrocytes in which deposition of cholesterol had
    occurred were present. At the highest dose, ALP activity was
    increased and BUN and creatinine were decreased. The incidence of
    blood in urine was increased in females at the mid- and high-dose
    level at week 26 and in high-dose females at termination. Absolute
    and relative liver, kidney and thyroid weights (spleen not measured)

    were increased at 1000 ppm. In females a tendency to an increased
    liver weight was observed at 200 ppm. The incidence of
    hepatocellular hypertrophy in the liver was increased in females at
    200 and in both males and females at 1000 ppm. In the same groups
    there was also an increase in tubular pigment in the kidney.
    Intranuclear inclusions and foci of pigmented histiocytes in the
    liver were observed in most high-dose dogs. The NOAEL in this study
    was 40 ppm, equivalent to 1 mg/kg bw/day, based on increased liver
    weight and histopathological changes at 200 ppm (Clay, 1991). 

         Groups of 4 beagle dogs/sex were administered 0, 40, 400 or
    1000 ppm dithianon (purity 95.3%) in the diet for 2 years. No
    effects were observed on clinical signs, mortality, body-weight,
    urinalysis or ophthalmoscopy. Food consumption was slightly
    depressed in high-dose dogs during the first 3 months of the study.
    At the highest dose level, Hb, PCV and RBC were decreased throughout
    the study and platelet counts tended to increase. At 1000 ppm,
    activity of ALP and ASAT were increased, as were levels of total
    protein and ß-globulin. At the end of the study, a decrease in
    glucose 6-phosphatase activity in the liver was seen in all female
    dogs and 1 male dog at 1000 ppm. Absolute and relative liver,
    kidney, pituitary, pancreas, thyroid weights were increased in dogs
    at 1000 ppm. Liver weight was also increased at 400 ppm. An
    irregular appearance of the exterior and cut surface of the liver
    was seen at 1000 ppm. At histopathology liver changes, consisting of
    hepatocyte enlargement associated with the presence of Kupffer cells
    which contained brown material, were observed at 1000 and sometimes
    at 400 ppm, more marked in females than in males. In the kidney
    tubular brown pigmentation was increased at 1000 ppm. The NOAEL in
    this study was 40 ppm, equivalent to 1 mg/kg bw/day, based on
    increased liver weight and histopathological changes at 400 ppm
    (Noel  et al., 1969)

         Additionally, stains for iron were used on all liver sections
    and bone marrow smears and sections were examined. The incidence and
    degree of iron accumulation was increased at 1000 ppm, particularly
    in females (Spicer, 1971b).

    Long-term toxicity/carcinogenicity studies

    Mice

         Groups of Crl:CD-1 (ICR)BR mice (51/sex/group) were fed
    concent-rations of 0, 20, 100 or 500 ppm dithianon (purity 92%) in
    the diet for 80 weeks. Observations included clinical signs,
    mortality body-weight, food and water consumption, haematology
    (differential white cell count), macroscopy, organ weights (brain,
    liver, thyroids, kidneys and testes of 20 mice/sex/group) and
    histopathology (about 40 tissues from control and high-dose mice and
    from mice that died or were killed in extremis, and tissues of

    lungs, liver and kidneys of all mice in the mid- and low-dose
    groups).

         Mortality was increased in high-dose males during the second
    part of the study. The incidence of fur staining in mice was
    increased at 500 ppm. Male terminal body-weight was lower in high-
    dose group. Relative kidney weight was increased in mice at 100 and
    500 ppm and relative brain weight was decreased in high-dose males.
    Histopathological kidney changes were observed in males at 500 ppm
    and in females at 100 and 500 ppm. Tumour incidence was not
    enhanced. The NOAEL in this study was 20 ppm, equal to 2.9 mg/kg
    bw/day, based on increased kidney weight and an increased incidence
    of chronic nephrosis (Brown, 1990). 

    Rats

         Groups of Charles River CD rats (35/sex/group) were fed diets
    containing 0, 20, 200 or 1000 ppm dithianon (purity 95%) for 2
    years. High-dose rats received 500 ppm dithianon in their feed
    during the first 4 weeks of the study. An interim kill was performed
    on 5 rats/sex/group at weeks 26 and 52 for clinical and pathological
    examinations. The remaining rats were sacrificed after 104 weeks
    except for the high-dose group in which the surviving males and
    females were killed at week 88 and 96, respectively, due to
    declining general condition. Observations included mortality,
    clinical signs, body-weight, food and water consumption, food
    efficiency, haematology, blood chemistry, urinalysis, macroscopy,
    organ weight and histopathology. Mid- and high-dose rats showed
    yellow discoloration of the fur during the first part of the study.
    Body-weight gain and food consumption were reduced in high-dose rats
    and in females at 200 ppm and water consumption was increased at the
    highest dose. PCV and Hb were decreased in rats at 1000 ppm
    throughout the study and PCV was also decreased at week 78 and 103
    in rats at 200 ppm. At the high-dose level, protein levels were
    increased in males and females and polyurea and proteinuria were
    occasionally found in this group towards the end of the study.
    Relative liver and kidney weight were increased in males and females
    at 1000 ppm and at 200 ppm in females. Relative thyroid weight was
    increased in high-dose males. No dose-related histopathological
    findings were observed. The NOAEL in this study was 20 ppm,
    equivalent to 1 mg/kg bw/day, based on effects on body-weight, red
    blood cells and liver and kidney at 200 ppm and higher (Wheldon  et
     al., 1969; Spicer & Benson, 1971). 

         Groups of Crl:CD(SD)BR rats (50/sex/group) were fed diets
    containing 0, 20, 120 or 600 ppm dithianion (purity 92%) for 104
    weeks. Observations included mortality, clinical signs, body-weight,
    food and water consumption, ophthalmoscopy, macroscopy, organ weight
    (adrenals, liver, ovaries, brain, kidneys and testes) and
    histopathology (about 40 tissues from control and high-dose rats and
    from rats that died or were killed in extremis, and tissues of

    lungs, liver and kidneys of all mid- and low-dose rats). Satellite
    groups (20/sex/group) were kept according to the same protocol but
    observations also included haematology, clinical chemistry
    (including thyroid hormone assays), urinalysis and thyroid weight.
    The incidence of fur staining and rough haircoat was increased in
    high-dose females. At high-dose, body-weight gain and food
    consumption were decreased in males up to week 24 and in females
    during the whole study. At 600 ppm and occasionally at 120 ppm, Hb,
    RBC, PCV and MCV were decreased in both sexes. T3 levels were
    decreased at the highest dose. At 120 and 600 ppm, glucose, BUN and
    œ-GT were increased in both sexes. Throughout the study cholesterol
    levels were decreased at the highest dose. No clear effects were
    observed in urinalysis. Relative kidney and liver weights were
    increased in both sexes at 600 ppm. The incidence of treatment-
    related kidney lesions (glomerulonephropathy and tubular nephrosis)
    was increased in both sexes at 600 ppm. In the kidneys of females at
    this dose level, an increased incidence of proliferative tubules,
    adenomas and carcinomas was observed. In females at 120 ppm,
    glomerulonephropathy and tubular nephrosis showed an increased
    incidence. The NOAEL in this study was 20 ppm, equivalent to 1 mg/kg
    bw/day, based on non-neoplastic kidney lesions observed at 120 ppm
    (Brown, 1991).

         Grasso (1991b) re-evaluated the kidney sections from female
    rats in the Brown, 1991 study. Similar changes were reported as in
    the original evaluation, including proliferative tubular changes
    (see Table 2). The incidence of kidney lesions was increased at 120
    and 600 ppm. A comparison of the kidney tumours found by Brown
    (1991) and Grasso (1991b) is given in Table 3. The re-evaluation
    reduced the number of adenomas, but the number of adenocarcinomas
    remained the same. In neither evaluation were tumours seen at doses
    below 600 ppm.

    Table 2.  Incidence of proliferative and some non-proliferative
              lesions in kidneys of female rats in a long-term/
              carcinogenicity study (according to Grasso, 1991b)
                                                                       
                                              Dose level (ppm)

                                     0          20       120       600
                                                                       

    Basophilic tubules               24        24        34        30
    Eosinophilic inclusions           0         2         3         9
    Atypical hyperplasia              0         0         4        10
    Proliferative tubules             0         0         0         6
    Adenoma                           0         0         0         7
    Adenocarcinoma                    0         0         0         2
                                                                       

    Table 3.  Tumour incidence in female rats in a long-term/
              carcinogenicity study according to Brown (1991) and a
              re-evaluation by Grasso (1991b)
                                                                       
                                              Dose level (ppm)

    Pathologist                      0         20        120       600
                                                                       

    Brown          adenoma           0         0         0         10*
                   carcinoma         0         0         0          2*

    Grasso         adenoma           0         0         0          7
                   carcinoma         0         0         0          2
                                                                       

    *    one animal had an adenoma and a carcinoma therefore 11 animals
         had tumours.

    Reproduction studies

         Groups of 10 male and 20 female rats received 0, 20, 200 or 500
    ppm dithianon (purity not specified) in the diet for 100 days before
    the initial mating. The treatment was continued throughout 3
    generations of 2 litters each. Observations were made on general
    condition and behaviour, food consumption (F0 generation only) and
    body-weight. Conception rate, gestation time, litter size, pup
    mortality, litter and mean pup weights at birth as well as at
    weaning (day 21) were studied. Autopsy, organ weight and
    histopathological examinations of selected organs were performed on
    all rats of the F3b generation. Grooming was impaired over the 3
    generations at 500 ppm and, in the F2b generation, at 200 ppm. At
    the highest dose, body-weight gain was depressed in all generations
    and in the 200 ppm of the F2b generation. Litter size at birth was
    not affected but throughout the study entire litters tended to be
    lost completely before weaning so litter size at weaning was
    generally lower in treated groups than in control groups (none dose-
    related). In the F1b matings (both litters) the incidence of
    litter loss was increased at 500 ppm. Over the three generations a
    tendency to a lower mean pup weight at birth as well as at weaning
    was observed at 500 ppm. Pup mortality was very variable in both
    treated and control groups but at the highest dose pup mortality
    seemed to be increased in the F1a, F2a and F2b litters. Liver
    and kidney weights of F3b pups were increased in males and females
    at 500 ppm. Kidney weight was also increased in 200 ppm males as was
    adrenal weight in 500 ppm males. The NOAEL was 20 ppm, equivalent to
    1 mg/kg bw/day, on the basis of decreased body-weight gain and
    increased kidney weight (Palmer & Readshaw, 1969). 

         Groups of 28 Crl:CD(SD)BR rats/sex were fed diets containing 0,
    35, 200 or 600 ppm dithianon (purity 91.6%). After 100 days of

    treatment, animals (F0 generation) were mated to start a 2-
    generation (1 litter/generation) study. F1 parents (24/sex/group)
    selected from F1a offspring were mated after 100 days to produce
    one litter. During the premating and mating period, body-weight gain
    and food consumption were decreased in F0 and F1 males and
    females at 600 ppm and in F0 females during gestation at the
    highest dose. No treatment-related effects were observed on clinical
    signs, mating and fertility indices in any of the parenteral rats,
    gestational length, pup weight, sex ratio and pup viability,
    physical and functional development and macroscopy of both parents
    and pups. Histopathology and weights of liver and kidneys were not
    available. The NOAEL in this study was 200 ppm, equivalent to 10
    mg/kg bw/day, based on decreased body-weight gain and food
    consumption at 600 ppm (Osterburg, 1991). 

    Special studies on embryotoxicity and/or teratogenicity

    Mice

         Groups of 24 pregnant NMRI mice were orally administered 0,
    3.3, 10, 30, or 90 mg dithianon/kg bw/day suspended in MHEC from
    days 6-15 of gestation. The dams were observed for clinical signs,
    food consumption and body-weight and were killed at day 19 of
    gestation. The number of implantations, resorptions, live and dead
    fetuses were recorded. Fetuses were weighed, sexed and examined for
    external and skeletal malformations. At 90 mg/kg bw/day, all dams
    died, 4 by day 7, and the last dam at day 15 of gestation. At
    autopsy pale parenchymatous organs were observed and the intestine
    was filled with haemorrhagic fluid. Body-weight was decreased at 10
    and 30 mg/kg bw/day and food consumption was decreased at 30 mg/kg
    bw/day during treatment. Fetal and placental weights were decreased
    at 30 mg/kg bw/day. Delayed ossification was observed at 10 and 30
    mg/kg bw/day. No malformations were observed. The NOAEL in this
    study for maternal and embryo-fetal toxicity was 3.3 mg/kg bw/day
    (Leuschner, 1976a).

    Rats

         In a preliminary study, groups of 8 mated Crl:CD(SD)BR rats
    were administered by gastric intubation 0, 20, 40 or 70 mg
    dithianon/kg bw/day suspended in CMC from days 6-15 post-coitum. At
    the end of the study, another 2 groups of 8 mated rats were treated
    according to the same protocol with 0 or 100 mg dithianon/kg bw/day.
    The dams were sacrificed on day 20 of gestation and the fetuses were
    removed and examined for external/visceral malformations. Excessive
    urination and increased water consumption was observed at 100 mg/kg
    bw/day. Maternal body-weight was decreased during days 6-9 of
    gestation and body-weight gain was depressed during treatment at 70
    and 100 mg/kg bw/day. At 100 mg/kg bw/day, post-implantation loss
    was increased and mean fetal weight was reduced. The incidence of
    external malformations was not increased. The NOAEL for maternal

    toxicity was 40 mg/kg bw/day and 70 mg/kg bw/day for embryotoxicity
    (Müller, 1989a).

         Groups of 25-32 mated female Crl:CD(SD)BR rats were dosed
    orally by gavage with 0, 20, 70 or 100 mg dithianon/kg bw/day
    (purity 91.6%) suspended in CMC from days 6-15 of gestation. Another
    2 groups of 25 mated female rats received 0 or 50 mg dithianon/kg
    bw/day and were treated according to the same protocol. On day 20
    the dams were sacrificed and the fetuses were removed and examined.
    Observations included mortality, clinical signs, body-weight, food
    consumption, number of corpora lutea, number and position of
    implantations, early and late resorptions, and live and dead
    fetuses. Fetuses were weighed, sexed and examined for external,
    skeletal or visceral abnormalities. At 100 mg/kg bw/day, 5/25 dams
    died and at 70 mg/kg bw/day, 1/32 dams died. Body-weight gain and
    food consumption were dose-relatedly reduced at 50, 70 and 100 mg/kg
    bw/day. Dams at 70 and 100 mg/kg bw/day revealed an increased
    incidence of abnormal findings in the stomach and large intestines
    at necropsy. Post-implantation loss and intra-uterine deaths were
    markedly and dose-relatedly increased at 50, 70 and 100 mg/kg
    bw/day. Mean fetal weight was reduced at the highest dose. No
    teratogenic effects were observed. The NOAEL for maternal and
    embryo-fetal toxicity was 20 mg/kg bw/day (Müller, 1991). 

    Rabbits

         Groups of 12 pregnant New Zeeland white rabbits were orally
    dosed by gavage with 0, 3.3, 10, 30 or 90 mg dithianon/kg bw/day
    suspended in MHEC from days 6-18 of pregnancy. The dams were killed
    on day 29 of pregnancy and fetuses were removed and weighed, sexed
    and examined for external and skeletal malformations. At the highest
    dose, all dams died prematurely between days 8 and 12 of pregnancy.
    At autopsy their parenchymatous organs appeared pale and the
    intestine was filled with fluid. In dams at 30 mg/kg bw/day food
    consumption was reduced during treatment and body-weight was
    decreased. The resorption rate and the post-implantation loss were
    increased at 30 mg/kg bw/day and placental weight was decreased.
    Slightly retarded ossification was observed in fetuses at 30 mg/kg
    bw/day. The NOAEL in this study for maternal and embryo-fetal
    toxicity is 10 mg/kg bw/day (Leuschner, 1976b).

         In a preliminary study, groups of 8-9 mated female New Zeeland
    white rabbits received 0, 10, 20 or 40 mg dithianon/kg bw/day
    (purity 91.6%) by oral gavage from days 6-18 of gestation. The dams
    were sacrificed on day 28 and the ovaries and uteri were removed and
    examined for number of corpora lutea, live and dead fetuses, early
    and late resorptions. Fetuses were weighed, sexed and externally
    examined. At the highest dose, two abortions were observed and one
    female showed 100% intra-uterine deaths at necropsy. During
    treatment, food and water consumption were reduced and body-weight
    was decreased from days 6-9 post-coitum at 40 mg/kg bw/day. The mean

    number of early resorptions and the post-implantation loss was
    increased at the highest dose. No external malformations were
    observed. The NOAEL for maternal and embryo-fetal toxicity is 20
    mg/kg bw/day (Müller, 1989b).

         Groups of 20 mated New Zeeland white rabbits were orally
    administered by gavage 0, 10, 25, or 40 mg dithianon/kg bw/day
    (purity 91.6%) in CMC from days 6-18 post-coitum. On day 28, the
    dams were sacrificed and fetuses were delivered by caesarean
    section. The number of corpora lutea, early and late resorptions and
    live and dead fetuses were recorded. The fetuses were weighed, sexed
    and examined for external, visceral or skeletal abnormalities.
    Mortality of dams was 0, 4, 3 and 4 in the control, low-, mid- and
    high-dose group, respectively. Three high-dose dams aborted; at
    necropsy no relevant findings were observed. Body-weight gain and
    food consumption were decreased at 25 and 40 mg/kg bw/day during
    treatment. At 10 mg/kg bw/day food consumption was also slightly
    decreased. An increased pre- and post-implantation loss was seen and
    the number of fetuses was reduced at the highest dose. In this study
    there were no indications for structural malformations associated
    with treatment. The NOAEL for maternal toxicity was 10 mg/kg bw/day
    and 25 mg/kg bw/day for embryo-fetal toxicity (Müller, 1990).

    Special studies on genotoxicity

          A number of genotoxicity tests had been carried out with
    dithianon. The results are summarized in Table 3. The only positive
    effect found was in the chromosomal aberration test  in vitro.
    However, an  in vivo test having the same endpoint, was negative.

    Special studies on nephrotoxicity

         Groups of CrL:CD(SD)BR rats (15/sex/group) were administered 0,
    120, 600 or 1080 ppm dithianon (purity 90%) in the diet for 7 days.
    Interim kills were performed on 5 rats/sex/group at days 2 and 4,
    the remaining animals were sacrificed at day 7. Food consumption was
    measured during treatment and body and kidney weights were recorded.
    Kidney and adrenal tissues as well as tissues from organs showing
    visible abnormalities were histopathologically examined. Sections of
    the cortex of the right kidney of all rats were examined by electron
    microscopy. Kidney samples of the control and high-dose groups after
    day 2 were not evaluated because of a technical problem. At both
    interim kills and at final sacrifice, body-weight was decreased at
    high dose and absolute and relative kidney weight were increased at
    all dose levels in a dose-related way. The incidence of pale kidneys
    was increased at the highest dose. After 7 days, kidneys showed
    hydropic degeneration of the proximal tubular cells and basophilic

        Table 3.  Results of genotoxicity assays on dithianon
                                                                                                 
                                     Concentration
    Test system    Test object       of dithianon       Purity  Results    Reference
                                                                                                 

    In vitro

    Ames test      S. typhimurium    1-333.3 µg/pl      91.6%   negativec  Müller &
                   TA1535, TA1537    in DMSOa                              Miltenburger,
                   TA1538, TA98      33.3-3333.3 µg/pl          negativec  (1986)
                   TA100             in DMSOb

    Ames test      S. typhimurium    0.1-5 µg/pld       95%     negativec  Shirasu  et al.
                   TA1535, TA100                                           (1977)
                   TA1538, TA98
                   TA1537

    Ames test      S. typhimurium    0.3-6.6 µg/pla     91.6%   negativec  Timm (1987)
                   TA 1537, TA98     10-2000 µg/plb             negativec
                   TA 1538;
                   TA1535            0.1-20 µg/pla              negativec
                                     10-2000 µg/plb             negativec
                   TA100             1.0-333.3 µg/pla           negativec
                                     10-3333.3 µg/plb           negativec

    Bacterial      E. coli WP2 hcr   0.1-5µg/pld        95%     negativec  Shirasu  et al.
    mutation                                                               (1977)
    assay

    Rec assay      B. subtilis       0.1-5 µg/pld       95%     negativec  Shirasu  et al.
                   H17, M45                                                (1977)

    Chromosome     Chinese hamster   25-600 ng/ml       91.6%   positivec  Heidemann
    aberration     V79 cells         in DMSOa                              (1988)
    assay                            500-5000 ng/ml
                                     in DMSOb                   positivec

    CHO/HGPRT      Chinese hamster   20-200 ng/ml       94.7%   negativec  Miltenburger
    mutation       V 79 cells        in DMSOa                              (1984)
    assay                            60-600 ng/ml               negativec
                                     in DMSOb

    UDS assay      Rat hepatocytes   0.1-20 µg/ml       91.6%   negativec  Timm (1986)
                                     in 1% DMSO

    Table 3 (Contd)
                                                                                                 
                                     Concentration
    Test system    Test object       of dithianon       Purity  Results    Reference
                                                                                                 

    In vivo

    Micro nucleus  NMRI (SPF)        1, 10 or 100       94.6%   negativec  Schultze
    test and       mice              mg/kg orally                          Schencking, M.
    in CMC                                                                 & Unkelbach, H.
                                                                           (1984)

    Host-mediated  S. typhimurium    100-400 mg/kg      95%     negativec  Shirasu  et al.
    assay          G46                                                     (1977)
                   in male ICR mice

    Chromosomal    Wistar rat        22.3, 106 and      91.6%   negativec  Volkner (1990)
    aberration     bone marrow       393.5 mg/kg bw
    assay                            in PEG 400
                                                                                                 

    a without metabolic activation
    b with metabolic activation
    c positive control(s) yielded expected positive results.
    d with and without metabolic activation
    
    tubules at 600 and 1080 ppm. In high-dose females the lesions were
    more severe and persistent. To a lesser extent these effects were
    already found after 4 days. Basophilic tubules represent tubular
    degeneration and reflect the extensive loss of proximal tubular
    cells through hydropic degeneration. From electron microscopical
    examination it appeared that the mitochondriae were especially
    involved. They were affected in a dose-related manner at most time
    points or doses investigated (in females even after day 2 at 120
    ppm). The mitochondria were swollen and had lost their cristae. The
    lesions were most severe in female rats treated with 1080 ppm. In
    addition, the proximal cells in female rats also showed inclusions
    of osmophilic material within the lyosomes. This suggests that lipid
    peroxidation had taken place (Price, 1991). A hypothesis was
    proposed that because dithianon shows a toxic effect to the kidneys,
    the increase in tumour incidence at a high toxic dose is most
    probably due to a non-genotoxic mechanism. The toxic action may be
    due to accumulation of a toxic glutathion conjugate in the kidneys,
    similar to the mechanism of action for hexachloro 1:3-butadiene.

    Special studies on skin and eye irritation

         A dose of 500 mg dithianon (purity 91-94%) moistened with
    distilled water was applied to the shaven intact and abraded skin of
    New Zeeland white rabbits for 24 h. No skin irritation up to 7 days
    after application was observed (Sommer & Frohberg, 1968b).

         Dithianon (purity 92%) was tested for irritation to shaven
    intact skin areas of 3 male and 3 female New Zeeland white rabbits
    under occlusive conditions. No edema or erythema were observed 24,
    48 and 72 h after a 4-h exposure (Ullmann, 1986b).

         Delan liquid (formulation with 25% dithianon) produced marked
    erythema and occasionally edema in 5 male and 5 female New Zeeland
    white rabbits when applied under occlusive conditions to the shaven
    intact and abraded skin for 24 hours. The effects were still present
    72 h after application (Sommer & Frohberg, 1968a).

         Nine New Zeeland white rabbits were given doses of 100 mg
    dithianon (purity 91-94%) into the conjunctival sac of the left eye.
    The eyes of 6/9 rabbits were washed, 3/9 after 2 seconds and the
    remaining 3/9 after 4 seconds. In the unwashed eyes redness,
    swelling and secretion of the conjunctiva, swelling of the iris,
    iridic injection and distinct turbidity of the whole cornea were
    observed. The pupils did not react to light. After 2 weeks, the
    conjunctival irritation was still apparent (slight) in 1 rabbit and
    the corneal changes were not reversible in 1/3 rabbits and only
    partly in the other 2/9. When the eyes of the rabbits were washed no
    signs of irritation were observed (Sommer & Frohberg, 1968b).

         Two New Zeeland white rabbits received a single installation of
    0.1 ml Delan liquid into the conjunctival sac of the left eye.
    Slight erythema, swelling and secretion of the conjunctiva developed
    in both eyes after 24 h. After a week, the conjunctiva still showed
    slight erythema and swelling (Sommer & Frohberg, 1968a).

         Application of 100 mg dithianon (purity 92%) into the left eyes
    of 3 male and 3 female New Zeeland white rabbits caused corneal
    opacity, iritis and inflammation of the conjunctivae. Severe corneal
    opacity was still observed in 2/6 rabbit by day 21 (Ullmann, 1987b).

    Special studies on skin sensitization

         Dithianon (purity 94.7%) had slight sensitizing properties when
    tested in Dunkin-Hartley albino guinea-pigs by the maximization test
    (Ullmann, 1985). This weak sensitization was also observed when
    dithianon (purity 91.6%) was tested by the open epicutaneous test
    (OET) on Dunkin-Hartley guinea-pigs (Ullmann & Kups, 1989a). After a

    re-challenge with 3 different dithianon impurities (D13, D14 or D25)
    in the OET with dithianon-sensitized guinea-pigs at least one
    impurity gave a positive result (Ullmann  et al., 1990). Pure
    dithianon (99.2%) failed to produce sensitization when also tested
    in the open epicutaneous test (Ullmann & Kups, 1989b).

         Dithianon was tested in albino guinea-pigs for photoallergenic
    properties. In one test, the observed weak sensitizing potential was
    enhanced by simultaneous UV-exposure (Heusener, 1986). This slight
    photo-allergenic effect was not established by two additional tests
    carried out with either dithianon technical (purity 91.6%) or
    dithianon pure (purity 99.6%) (Ullmann  et al., 1989d, 1989e).

    COMMENTS

         After oral administration to rats, goats and hens, dithianon
    was rapidly absorbed, distributed and excreted. Five days after its
    oral administration to rats almost all of the administered
    radioactivity had been eliminated, 62-70% via faeces and 30-34% via
    urine. Only a small proportion of the dose was recovered in tissue,
    with highest levels in kidneys, the gastrointestinal tract and whole
    blood. Up to 10% had been excreted in the bile after 48 h. Dithianon
    was quickly metabolized to a number of polar metabolites, which have
    defied identification due to their lability. Less than 1% unchanged
    dithianon was found in the faeces.

         The acute oral toxicity of dithianon was moderate in mice and
    rats. WHO has classified dithianon as slightly hazardous (WHO,
    1992).

         Short-term toxicity studies with mice, rats and dogs indicated
    that the kidney is the primary target organ. Increased kidney
    weight, hydropic degeneration of the proximal tubular cells and
    basophilic tubules were demonstrated in the rat.

         In a 90-day study in rats, dithianon was administered at
    dietary concentrations of 0, 30, 180, or 1080 ppm. At high
    concentrations effects on red blood cells, an increase in liver and
    kidney weight and histopathological changes in the kidney were
    noted. The NOAEL was 30 ppm, equal to 2.5 mg/kg bw/day for males and
    3 mg/kg bw/day for females based on increased kidney weight.

         Three studies with dogs were reviewed. In a 90-day study,
    dithianon was fed at dietary concentrations of 0, 40, 200, or 1000
    ppm. The NOAEL was 200 ppm, equal to 3 mg/kg bw/day based on
    increased organ weights in the high-dose group. In both the 52-week
    (0, 40, 200 or 1000 ppm) and 2-year (0, 40, 400 or 1000 ppm) studies
    dithianon caused effects on red blood cells increased liver and
    kidney weights, hepatocellular hypertrophy and tubular pigmentation
    in the kidney. In each study the NOAEL was 40 ppm, equivalent to 1
    mg/kg bw/day, based on increased liver weight and histopathological
    changes at 200 ppm and 400 ppm, respectively.

         In an 80-week long-term feeding study in mice at dietary
    concentrations of 0, 20, 100, or 500 ppm, the NOAEL was 20 ppm,
    equal to 2.9 mg/kg bw/day, based on increased kidney weight and an
    increased incidence of chronic nephrosis. Two 2-year rat studies at
    dietary concentrations of 0, 20, 200, or 1000 ppm and 0, 20, 120, or
    600 ppm, respectively, were conducted. In the first study, only
    effects on body-weight, red blood cells and liver and kidney weight
    were observed at 200 ppm and higher, equivalent to 10 mg/kg bw/day.
    In the second study these effects were confirmed together with a
    number of histo-pathological renal changes, especially in females at
    600 ppm. At this level kidney adenomas and adenocarcinomas were also

    found. The NOAEL was 20 ppm, equivalent to 1 mg/kg bw/day, based on
    non-neoplastic kidney lesions observed at 120 ppm. The Meeting
    concluded that dithianon induced kidney tumours in females rats at
    600 ppm. It has been hypothesized that tumour induction is secondary
    to other renal changes seen in rats.

         Two reproduction studies in rats were reviewed. In the first
    study at dietary concentrations of 0, 20, 200, or 500 ppm dithianon,
    the body-weight gain of parents and pups was decreased, pup
    mortality was increased and liver and kidney weight were increased.
    The NOAEL was 20 ppm, equivalent to 1 mg/kg bw/day based on
    decreased body-weight gain and increased kidney weight. In the
    second study (dietary concentrations of 0, 35, 200, or 600 ppm) the
    NOAEL was 200 ppm, equivalent to 10 mg/kg bw/day based on decreased
    body-weight gain and food consumption at 600 ppm.

         Teratogenicity studies were conducted with mice, rats, and
    rabbits. In mice, maternal toxicity and delayed ossification were
    observed. The NOAEL for both effects was 3.3 mg/kg bw/day. In rats,
    maternal toxicity, post-implantation loss and reduced fetal weight
    were observed at doses of 50-100 mg/kg bw/day. The NOAEL was 20
    mg/kg bw/day. In a study in rabbits, 30 mg/kg bw/day caused maternal
    toxicity, post-implantation loss and retarded ossification. The
    NOAEL was 10 mg/kg bw/day. In a second study in rabbits, maternal
    toxicity was observed at 25 and 40 mg/kg bw/day but not at 10 mg/kg
    bw/day. The NOAEL for fetotoxicity was 25 mg/kg bw/day. Teratogenic
    effects were not observed in any of the studies.

         After reviewing the available genotoxicity data, the Meeting
    concluded that dithianon was not genotoxic.

         The Meeting concluded, after consideration of the long-term
    toxicity studies and the genotoxicity data, that dithianon did not
    pose a carcinogenic hazard for humans.

         An ADI was allocated, based on NOAELs in two-year studies in
    rats and dogs, using a 100-fold safety factor.

    TOXICOLOGY EVALUATION

    Level causing no toxicological effect

         Mouse:    20 ppm in the diet, equal to 2.9 mg/kg bw/day (80-
                        week study)

         Rat:      20 ppm in the diet, equivalent to 1 mg/kg bw/day (2-
                        year studies)

         Dog:      40 ppm in the diet, equivalent to 1 mg/kg bw/day (one
                        and two-year studies).

    Estimate of acceptable daily intake for humans

         0-0.01 mg/kg bw

    Studies which will provide information valuable in the continued
    evaluation of the compound

    1.   Characterization of the metabolites of dithianon in mammals and
         plants.

    2.   Clarification of the mechanism of nephrotoxicity and induction
         of kidney tumours.

    3.   Observations in humans.

    REFERENCES

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    of dithianon (technical) of E. Merck (India) Ltd., Bombay, Institute
    of Toxicology. Submitted to WHO by Shell International Chemical
    Company Ltd., Shell Centre, London, UK.

    Brown, D. (1987) Dithianon, 4-week oral (dietary administration)
    dose range-finding study in the mouse. Unpublished report No. 5478-
    460/15 dated July 1987 from Hazleton UK. Otley Road, Harrogate,
    England. Submitted to WHO by Shell International Chemical Company
    Ltd., Shell Centre, London, UK.

    Brown, D. (1989) Dithianon: 21-day dermal toxicity study in the rat.
    Unpublished report 5774-460/19A from Hazleton UK, Otley Road,
    Harrogate, England. Submitted to WHO by Shell International Chemical
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    Brown, D. (1990) Dithianon: 80-week oral (dietary administration)
    carcinogenicity study in the mouse. Unpublished report No. 6096-
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    Brown, D. (1991) Dithianon: 104 week oral (dietary administration)
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    Cheng, Th. (1990a) Five-day repeated dose of 14C-dithianon in
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    Cheng, Th. (1990b) Five-day repeated dose of 14C-dithianon in
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    Clay, H. (1991) Dithianon: 52 week oral (dietary administration)
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    Grasso, P. (1991a) Summary report on short-term studies on dithianon
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    Hawkins, D.R., Elsom, L.F., Thomson, J.K & Whitby, B.R.(1989) The
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    Heidemann, A. (1988) Chromosome aberration assay in Chinese hamster
    V79 cells  in vitro with dithianon technical. Unpublished report
    CCR project 122106 dated August 08, 1988 from CCR Cytotest Cell
    Research GmbH & Co.KG Darmstadt, Germany. Submitted to WHO by Shell
    International Chemical Company Ltd., Shell Centre, London, UK.

    Heusener, A. (1986) Investigation for photoallergenic potential in
    albino guinea pigs. Unpublished report dated August, 25, 1986
    Bericht nr. 4/56/86 from Merck, Darmstadt, Germany. Submitted to WHO
    by Shell International Chemical Company Ltd., Shell Centre, London,
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    Lackenby, F. (1987) Dithianon: 5 day dermal range-finding study in
    the rat. Unpublished report No. 5537-460/18 dated September 1987
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    Leuschner, F. (1976a) Influence of dithianon on the pregnant mouse
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    Leuschner, F. (1976b) Influence of dithianon technical lot 45 (1,2-
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    Miltenburger, H.G. (1984) Mutations affecting the hypoxanthine-
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    Deutschland GmbH, Münster, Germany. Submitted to WHO by Shell
    International Chemical Company Ltd., Shell Centre, London, UK.

    Müller, W. (1990) Dithianon oral (gavage) teratogenicity study in
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    Müller, E.W. (1991) Dithianon oral (gavage) teratogenicity study in
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    Müller, E.W. & Miltenburger, H.G. (1986) Induction of gene mutations
    in mutant strains of  Salmonella typhimurium (Ames test) without
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    dithianon, batch No. 15C/86 in beagle dogs. Unpublished report No.
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    Sakamoto, Y., Matsumoto, K. & Okami, H. (1975) Acute toxicological
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    Sommer, S. & Frohberg, H. (1968b) Trial for primary irritation of
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    Sommer, S. & Frohberg, H. (1969) Trial for acute toxicity in mice
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    Spicer, E.J.F. (1971a) Delan 75WP subacute inhalation toxicity in
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    Ullmann, L. (1985) Test for delayed hypersensitivity in the albino
    guinea-pig with dithianon technical (Guinea-pig maximization test)
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    Ullmann, L. (1986a) Acute dermal toxicity study with dithianon in
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    Ullmann, L. (1987a) Acute oral toxicity study with dithianon in
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    Ullmann, L. (1987b) Primary eye irritation study with dithianon in
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    Ullmann, L. & Kups, A. (1989a) Determination of skin irritation and
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    See Also:
       Toxicological Abbreviations