WORLD HEALTH ORGANIZATION

    WHO Food Additives Series 1972, No. 1




    TOXICOLOGICAL EVALUATION OF SOME 
    ENZYMES, MODIFIED STARCHES AND 
    CERTAIN OTHER SUBSTANCES




    The evaluations contained in this publication were prepared by the
    Joint FAO/WHO Expert Committee on Food Additives which met in Rome,
    16-24 June 19711





    World Health Organization

    Geneva

    1972





                   

    1 Fifteenth Report of the Joint FAO/WHO Expert Committee on Food
    Additives, Wld Hlth Org. techn. Rep. Ser., 1972, No. 488; FAO
    Nutrition Meetings Report Series, 1972, No. 50.

    The monographs contained in the present volume are also issued by the
    Food and Agriculture Organization of the United Nations, Rome, as FAO
    Nutrition Meetings Report Series, No. 50A

    (c) FAO and WHO 1972



    MICROBIAL GLUCOSE OXIDASE

    Biological data

    Source

    This enzyme preparation is prepared from some varieties of
    Aspergillus niger.

    Acute toxicity

    None available.

    Short-term studies

    None available.

    Long-term studies

    None available.

    Special studies

    Groups of 5 ducklings received in their diet either 0, 1, 5 or 10 per
    cent. of enzyme for 29 days.  Growth, feed efficiency, behaviour and
    survival were similar in all groups except the 10 per cent. level
    which showed some growth retardation.  No gross liver lesions or
    differences in mean liver weight were noted.  Histopathology was
    normal.  No toxic element was noted (F.D.R.L., 1963).

    Comments

    Aspergillus niger is a contaminant of food.  The available
    information appears to indicate that it is not pathogenic to man.  A
    duckling study is available and may serve to exclude the possibility
    of aflatoxin or related mycotoxin production occurring in this strain.

    EVALUATION

    Temporarily not limited.*

    Further work required by 1972

    Adequate 90-day study in the rat.

    REFERENCE

    F.D.R.L. (1963) Unpublished report No. 846OOd of the Food and Drug
    Research Laboratories submitted by Miles Chem. Co.

                   
    *Except for good manufacturing practice.
    

    See Also:
       Toxicological Abbreviations