WORLD HEALTH ORGANIZATION WHO Food Additives Series 1972, No. 1 TOXICOLOGICAL EVALUATION OF SOME ENZYMES, MODIFIED STARCHES AND CERTAIN OTHER SUBSTANCES The evaluations contained in this publication were prepared by the Joint FAO/WHO Expert Committee on Food Additives which met in Rome, 16-24 June 19711 World Health Organization Geneva 1972 1 Fifteenth Report of the Joint FAO/WHO Expert Committee on Food Additives, Wld Hlth Org. techn. Rep. Ser., 1972, No. 488; FAO Nutrition Meetings Report Series, 1972, No. 50. The monographs contained in the present volume are also issued by the Food and Agriculture Organization of the United Nations, Rome, as FAO Nutrition Meetings Report Series, No. 50A (c) FAO and WHO 1972 DISTARCH GLYCEROL Treatment with epichlorhydrin under akaline conditions up to 0.3 per cent. tends to produce cross-linking at a rate of one glycerol diether linkage per 220 - glycopyranose units. Biological data Biochemical aspects Much evidence in the literature points to etherified starches being somewhat resistant to enzymic degradation. Probably small fragments containing the ether linkage will remain and will not be absorbed readily. Any material absorbed is likely to be excreted by the kidney. No direct experimental evidence exists for this particular hypothesis (French, 1960). The metabolic fate of methylglucose is cited as collateral evidence. Although methylglucose is absorbed by rats (Sols, 1956) no demethylation is believed to occur (Csaky & Wilson, 1956) and excretion is essentially quantitative (Csaky & Glenn, 1957). The in vitro digestibility by amyloglucosidase of distarch glycerol (0.3 per cent. epichlorhydrin) was shown to be 98.3 per cent. of that of unmodified starch (Kruger, 1970). The caloric values of two starches treated at levels of 0.07 per cent. and 0.50 per cent. epichlorhydrin were estimated in groups of 10 weanling male rats by comparing the weight gain obtained after feeding for 28 days a basal diet supplemented with 3 g of each starch against a dose-response curve obtained by feeding daily sucrose supplements of 0, 1.5 g, 3 g, 4.5 g and 6 g equivalent to approximately 0, 6, 12, 18 and 24 calories. Distarch glycerols were only slightly lower in caloric value than unmodified starch, the differences not being significant. Short-term studies Rat Three groups of 10 male and 10 female rats were fed for 90 days unmodified starch or starch treated with 0.07 per cent. or 0.50 per cent. epichlorhydrin at 71 per cent. of their diet. Growth rate, appearance, behaviour and food intake were similar in all 3 groups. Haematological investigations showed no abnormalities related to the feeding of modified starches. Non-protein nitrogen blood levels and blood sugar levels remained normal in all groups. The weights of all major organs were normal and no abnormal gross or histopathological changes related to treatment were seen. Long-term studies None available. Comments The feeding studies with rats show that the modified starch is well utilized. The available evidence for the group of modified starches considered suggests that caecal enlargement without associated histopathological changes is of little toxicological significance. The short-term rat study shows no adverse effects at the high dietary level used. No long-term study on this modified starch is available and collateral evidence from the long-term and reproduction studies with hydroxypropyl distarch glycerol, a more highly modified starch, indicates that the glycerol linkage is causing no adverse effects. EVALUATION Temporarily not limited.* Further work required by 1973 Histological report to complete the 2-year rat study on hydroxypropyl distarch glycerol. Histological report to complete the reproduction study on hydroxypropyl distarch glycerol. REFERENCES Csáky, T. Z. & Wilson, J. E. (1956a) Biochem. Biophys. Acta., 22, 185 Csáky, T. Z. & Glenn, J. E. (1956b) Amer. J. Physiol., 188, 159 French, D. (1960) Unpublished report Kruger, L. (1970) Unpublished reports Nos 405 & 406 submitted by National Starch and Chemical Co. Oser, B. L. (1961) Unpublished report of Food and Drug Research Laboratories Inc., submitted by National Starch and Chemical Corp. Sols, A. (1956) Rev. esp. Fisiol., 12, 17 * Except for good manufacturing practice.
See Also: Toxicological Abbreviations Distarch glycerol (FAO Nutrition Meetings Report Series 46a) Distarch glycerol (WHO Food Additives Series 5) DISTARCH GLYCEROL (JECFA Evaluation)