MODIFIED CELLULOSES (Ethyl cellulose, ethyl hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, methyl ethyl cellulose, sodium carboxymethyl cellulose). 1. EXPLANATION Modified celluloses were reviewed at the fifth, seventh, tenth, thirteenth, seventeenth, twenty-sixth, twenty-seventh and thirtieth meetings of the FAO/WHO Joint Expert Committee on Food Additives. (Annex 1, references 5, 7, 13, 19, 32, 59, 62, and 73). At the seventeenth meeting a group ADI of 0-25 mg/kg bw was allocated for the five previously reviewed modified celluloses which included methyl cellulose, methyl ethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, and sodium carboxymethyl cellulose. A monograph was prepared discussing these 5 compounds (Annex 1, reference 33). The use of microcrystalline cellulose was not included in this group ADI: no limitation other than that imposed by good manufacturing practice was deemed necessary for the use of microcrystalline cellulose as an additive (Annex 1, reference 26). At the twenty-sixth and twenty-seventh meetings of the committee ethyl cellulose and ethyl hydroxyethyl cellulose, respectively, were reviewed and added to the group ADI of 0-25 mg/kg bw. A monograph on ethyl hydroxyethyl cellulose was prepared after the thirtieth meeting (Annex 1, reference 74). Since the previous evaluation, additional data have become available and are summarized and discussed in the following monograph. The previously published monographs have been incorporated into the document below. 2. BIOLOGICAL DATA ETHYL CELLULOSE 2.2 Toxicological studies 2.2.1 Acute toxicity Species Sex Route LD50 (mg/kg bw) Reference Rat ? Oral 5,000 Moreno, 1977 Rabbit ? Dermal > 5,000 Moreno, 1977 2.2.2 Short-term studies 2.2.2.1 Rats No adverse effects were reported in 80 rats fed a diet containing 1.2% ethyl cellulose, which is equivalent to 182 mg/kg bw/day (Hake & Rowe, 1963). 2.2.3 Long-term/carcinogenicity studies No data available. 2.2.4 Reproduction studies No data available. ETHYL HYDROXYETHYL CELLULOSE 2.2 Toxicological studies 2.2.1 Acute toxicity Species Sex Route LD50 (mg/kg bw) Reference Rat ? Oral 5,000 - 10,000 Cuthbert, 1975 The sensitization potential of 4 types of ethyl hydroxyethyl celluloses was negative in the guinea pig by Kligman's maximization test using positive and negative control groups. In albino rabbits, primary skin irritation was evaluated on abraded skin using a Draize type of procedure. The ethyl hydroxyethyl celluloses produced only very mild irritant reactions, with primary indices between 0.75 and 1.0. The control material, sodium carboxymethyl cellulose, produced the greatest irritant reactions with a resulting index of 1.42 (Cuthbert, 1975). 2.2.2 Short-term studies 2.2.2.1 Rats Ethyl hydroxyethyl cellulose was administered to Charles River CD rats (20 males and 20 females/group) by continuous dietary incorporation at dose levels of 0, 250, 1375 or 2500 mg/kg bw/day for 90 consecutive days. All the animals were observed daily and all signs of toxicity or behavioral changes were recorded. Body weight and food consumption were recorded weekly. Ophthalmoscopy and hematology analyses were carried out prior to termination. After 90 days of treatment the animals were killed and macroscopic examination was carried out on all tissues from animals in the control and high-dose groups. A statistically-significant increase in cumulative food consumption was recorded in male rats from the high-dose group throughout the study (weeks 1, 4, 8 and 12) and female rats of the high-dose group at weeks 4, 8 and 10. The increased food consumption was considered to represent compensation for the dietary inclusion of the test substance. A corresponding marginal decrease in the efficiency of food utilization was noted in rats of this treatment group throughout the study. After adjustment for final body weight, a small decrease in liver weight was apparent in male rats of the high-dose group in comparison with control values. No changes in the livers of these rats were apparent on histological examination. In all other respects, including general health, body weight, ophthalmoscopy, hematology, biochemistry, and macroscopic and microscopic pathology, rats receiving ethyl hydroxyethyl cellulose were similar to the controls (Elliot et al., 1985). 2.2.3 Long-term/carcinogenicity studies No data available. 2.2.4 Reproduction studies No data available. 2.3 Observations in man Ethyl hydroxyethyl cellulose was administered in a dose of 1.0 to 1.5 g, three times a day for at least 2 months, in a study of 85 male and female ambulatory patients (age 21-75 yr) with intestinal problems. Sixty-eight patients remained on the treatment. X-ray contrast media were used to study tablet disintegration in several patients. The disintegration time was greater than 20 minutes. Aside from minor abdominal discomfort in a few patients, no toxicity was noted, and restoration to normal bowel movement was seen (Tomenius, 1957). HYDROXYPROPYL CELLULOSE 2.1 Biochemical aspects 2.2.1 Absorption, distribution and excretion When 250 or 1,000 mg/kg bw of [14C]hydroxypropyl cellulose was administered to rats in a 5% aqueous solution, radioactivity no greater than 0.01% of the administered dose was detected in organs, urine and expired air. Recovery of activity in the feces varied from 98.32 to 102.7%. Hence orally ingested material is not absorbed from the gastrointestinal tract of the rat and is excreted quantitatively in the feces, principally in the first 48 hours. To check on enterohepatic circulation, two additional rats with ligated bile ducts were administered 1,000 mg/kg bw of radio-labelled material. Bile was collected for 72 hours, but no significant activity was found (Industrial Bio-Test Lab., 1964, not validated). [14C]hydroxypropylcellulose (12.28% hydroxypropyl group, 2.74 uCi/mg) was administered to 3 male and 3 female rats of Wistar-Imamichi Strain, weighing approximately 250g, at a dose of 1.3 g/kg bw. Urine and feces were collected for 96 hours, and the residual radioactivity was measured in the tissues of the rats after sacrifice. The bile duct was cannulated for bile collections. The mean urinary excretion of radioactivity over 96 hours was 2.63% in males and 1.50% in females. The mean fecal excretion was 68.7% and 97.3% in males, and 62.4% and 96.8% in females, over 24 and 96 hrs, respectively. Total radioactivity in urine and feces over 96 hrs was 99.9% in males and 98.3% in females. Cumulative biliary excretion over 24 hrs was .015% for males and .0024% for females (Kitagawa et al., 1976a). Gel filtration chromatographic patterns of urine were inconclusive for identification of the single peak of radioactive material. The elution position showed a molecular weight slightly higher than glycerol or glucose and it was found at a different position than propylene glycol, which was present at a level of less than 2% in the administered material. No radioactivity was detectable in tissues other than the liver and kidney. The highest radioactivity in the liver was 1.5% of the dose in male rats at 12 hrs and 0.026% at 24 hrs in females (Kitagawa et al., 1976a). 2.2 Toxicological studies 2.2.1 Acute toxicity Species Sex Route LD50 (mg/kg bw) Reference Rat ? Oral 10,200-15,000 Industrial Bio- Test Lab., 1962 (not validated) Kitagawa, et al., 1976b 2.2.2 Short-term studies 2.2.2.1 Rats Groups of 5 male and 5 female rats received in their diet 0.2%, 1.0% and 5.0% of hydroxypropyl cellulose for 90 days. Controls received unmodified cellulose at the same levels. No differences were observed between test and control animals as regards mortality, growth, food utilization, urinalysis, hematological indices, organ weight, gross pathology and histopathology. At higher dietary levels, increased food consumption and decreased food utilization was observed, probably due to dietary dilution (Industrial Bio-Test Lab., 1963, not validated). Groups of 10 male and 10 female young adult Wistar rats were fed hydroxypropyl cellulose in 1% gum arabic at doses of 0, 1.5, 3.0, or 6.0 g/kg bw/day for 30 days and for 6 months. After 30 days, no effects were observed on body weight and food consumption, serum chemistry, urinalysis, or histopathology. In females, liver, kidney and brain weights were decreased at 3.0 g/kg bw/day, but the decrease did not show a consistent dose-response relationship. After 6 months, decreased body weight was noted at the high dose level, which was statistically significant for females. No effects related to dosage were noted on food consumption, serum chemistry, urinalysis, or histopathology. The hemoglobin level of male high- and mid-dose rats was reduced. There were sporadic increases or decreases in organ weights of a few groups without a dose relationship or associated pathological change (Kitigawa et al., 1976b). 2.2.2.2 Chickens Day-old Arbor Acres chicks were apportioned into groups of 10 and fed low-fat, high-fat or high-protein diets containing 2% hydroxypropyl cellulose or one of a variety of vegetable gums or other polysaccharides for 3 wk. The control diet contained 2% cellulose. Growth of chicks on the hydroxypropyl cellulose diet was depressed by 8% relative to the control diet, but feed intake, nitrogen retention and fat absorption were unchanged (Kratzer et al., 1967). 2.2.3 Long-term/carcinogenicity studies No data available. 2.2.4 Reproduction studies No data available. 2.2.5 Special studies on teratogenicity 2.2.5.1 Rats Groups of nulliparous female Wistar rats were mated to give 36-37 pregnant rats per dose group. Hydroxypropyl cellulose was administered daily by gavage in 1% gum arabic at dose levels of 0, 200, 1000, or 5000 mg/kg bw/day between days 7 and 17 post-mating. On day 21 of gestation, 21-24 females were subjected to Cesarean section. Corpora lutea, implantations, viable and dead fetuses, and resorbed embryos were counted and positions of implantations were observed. All viable fetuses were individually weighed and examined for abnormalities. Two to three fetuses per group were examined for skeletal abnormalities, and the remainder were examined for visceral abnormalities. Twelve to fifteen dams were allowed to deliver spontaneously. Viable pups and stillborns were counted, and records taken of body weight, sex, and presence of external anomalies. General behavior of pups was observed during nursing and individual body weights were recorded at delivery and weaning. Times for separation of lower incisors and and separation of eyelids were noted. The pups were weaned at the 28th day after birth. Each weanling was examined for general behavior and nervous reflexes. Skeletal examination was done by soft X-ray. One male and one female per group were killed and wet weights of the brain, heart, lung, liver, spleen, kidney, thymus, adrenal, testes, epididymis, prostate, ovary, pituitary and thyroid were obtained. Remaining weanlings were observed for 5 weeks for body weight gain, and at maturity for conditioned avoidance response and reproductive ability. Both mean litter weight and percent pre-implantation loss were significantly increased in the high dose group. The percent of skeletal variations was significantly increased for the mid dose only. At maturity, the progeny showed no effects on reflex behavior or reproductive ability (Kitagawa et al., 1978a). 2.2.5.2 Rabbits Groups of 11-12 pregnant Himalayan rabbits received oral doses of hydroxypropyl cellulose by gavage in 1% gum arabic at levels of 0, 200, 1000, or 5000 mg/kg bw daily from days 6-18 of pregnancy. Cesarean sections were performed on the 29th day of pregnancy and all fetuses were examined for skeletal and organ malformations. Up to the 18th day of study a slight body weight loss was noted in the high dose group. A slight decrease in the number of implants, not dose-related, was recorded in treatment groups. The resorption rate was significantly decreased only in the intermediate dosage group. The mean fetal viable weight was not different between groups. The pre-implantation loss was significantly increased in the 5000 mg/kg bw/day group. The incidence of malformations was comparable to historical controls and was not dose-related (Kitagawa et al., 1978b). HYDROXYPROPYL METHYL CELLULOSE 2.1 Biochemical aspects 2.1.2 Absorption, distribution and excretion The disposition of orally administered 500 mg [14C]hydroxypropyl methyl cellulose/kg bw (approx. 25 uCi in 3 ml) was examined in 3 male and 3 female Sprague-Dawley rats either as a single dose, or as a series of 5 consecutive doses. Radioactivity associated with the single dose was recovered from the feces (>99%), urine (approx. 1%), carcass and tissues (approx. 0.2%), expired air (0.07%), and bile (0.05%). The excretory half life from plasma was approximately 2 hr. The tissue accumulating the most radioactivity was the gastrointestinal tract; 0.53% of the administered dose consisted of cellulose units with an average molecular weight < 1000, which was thought by the authors to be accounted for entirely (approx. 0.56% administered dose) in the urine as methyl ethers of glucose and oligomers. Radioactivity recovered after 5 consecutive doses was found primarily in the feces (97% in males, 102% in females) with trace amounts in the urine (approx. 1.0%) and with no evidence of tissue accumulation (Gorzinski et al., 1986). The cecal contents from 2 male Wistar rats were incubated in vitro at 37°C for 0, 6, 12, 24, and 48 hr in complex medium broth with 2 mg/ml of hydroxypropyl methyl cellulose. Total viable bacteria, carbohydrate utilization and free reducing-ends were determined. Hydroxypropyl methyl cellulose remained almost completely unfermented (5% in 48 hr, no further change by 7 days), and there was no increase in reducing-ends (i.e., no degradation of the cellulose polymers), or in viable bacterial counts compared to the control incubations (Wyatt et al., 1988). 2.2 Toxicological studies 2.2.1 Acute toxicity Species Sex Route LD50 (mg/kg bw) Reference Mouse ? i.p. 5,000 Hodge et al., Rat F oral >1000 mg/kg 1950 CTFA, 1978a ? i.p. 5,000 Hodge et al., 1950 2.2.2 Short-term studies 2.2.2.1 Rats Groups of 10 male and 10 female weanling rats were fed diets containing 0, 2, 10 and 25% hydroxypropyl methyl cellulose, type B, for 30 days. Only in the highest dose were interference with body weight gain and diarrhea observed. There were no histological lesions nor were there abnormal findings in urine and blood (Hodge et al., 1950). Groups of 10 male and 10 female young rats were fed 0, 1, 3, 10 and 30% of hydroxypropyl methyl cellulose, type A, for 121 days. Body weight gain was markedly retarded at the 30% level, with 50% mortality attributed to undernutrition. Only the male rats showed slight body weight gain retardation at the 10% dietary level, while the weight gain was normal at the lower levels. Histological examination of internal organs revealed no abnormalities in any of the five groups (McCollister & Oyen, 1954). Groups of 10 male and 10 female young rats were fed diets containing 0, 0.3, 1, 10 and 20% of hydroxypropyl methyl cellulose, type C, for 90 days. At the 20% level both sexes showed marked retardation of body weight gain, with 30% mortality. At the 10% level male rats only showed slight but significant weight gain retardation. At the lower levels there were no adverse effects. The microscopic appearance of tissues was normal at all levels (McCollister et al., 1961). Groups of 10 male and 10 female young rats were fed 0, 0.3, 1, 3, 10 and 20% of hydroxypropyl methyl cellulose, type D, for 84 days. No adverse effects were noted with female rats at all levels. Male rats showed a definite retardation of body weight gain at 20% level and a slight retardation at 10%. Organ weights and gross and microscopic examination revealed no adverse effects (McCollister et al., 1961). Groups of 10 male and 10 female young rats (unknown strain) were fed diets containing 0, 1, 3 and 10% hydroxypropyl methyl cellulose (higher viscosity, 31,800 cP) and 0, 1, 3, and 10% hydroxypropyl methyl cellulose (lower viscosity, 8,480 cP) for 92 days. No adverse effects were observed as judged by mortality, growth, general appearance and behavior, body weights, food consumption, hematological and serum chemistry analysis, organ weights and gross and histological examination (McCollister & Copeland, 1967). Groups of 10 male and 10 female Dow-Wistar rats were fed diets containing 0, 1, 3 and 10% low viscosity (10 cP) hydroxypropyl methyl cellulose for 90 days. Groups of 10 male and 10 female Sprague-Dawley rats were fed diets containing 0, 3 and 10% high viscosity (4000 cP) hydroxypropyl methyl cellulose for 90 days. No evidence of toxicity was observed in either strain of rats as judged by mortality, body weights, food consumption, urine and hematological analyses, serum chemistry, organ weights and gross and histological examination (McCollister et al., 1973). Groups of 15 male and 15 female Sprague-Dawley rats were fed diets containing 0, 1 and 5% low viscosity (4.22 cP) hydroxypropyl methyl cellulose for 90-91 days. No evidence of toxicity was observed in rats as judged by mortality, body weight, food consumption, urine and hematology analyses, serum chemistry, organ weights and gross and histological examination (Schwetz et al., 1973). Groups of 5 male Wistar rats were fed diets containing either 0 or 100 g hydroxypropyl methyl cellulose/kg bw for 12 days. The hydroxypropyl methyl cellulose diet led to an enlargement of the cecum and colon associated with increased contents and tissue weight. The density of bacteria in the cecum and colon of animals fed hydroxypropyl methyl cellulose was significantly reduced over the fibre-free controls. The authors concluded that the cecal and colonic hypertrophy is a physical response to the increased content bulk (Wyatt et al., 1988). 2.2.2.2 Rabbits Groups of 6 rabbits were fed diets containing 0, 10 and 25% hydroxypropyl methyl cellulose, type B, for 30 days. The group on the highest dose maintained, but did not increase, their body weight. Normal results were obtained from urine and blood analyses, comparison of organ weights and histological examination (Hodge et al., 1950). 2.2.2.3 Dogs Groups of two dogs were fed for one year 0.1, 0.3, 1.0 and 3.0 g/kg bw/day of hydroxypropyl methyl cellulose, type B, without effect on body and organ weights, urine, blood and microscopic appearance of internal organs. One dog fed 25 g/kg bw/day for 30 days suffered no ill effects. Another dog fed 50 g/kg bw/day for 30 days exhibited some diarrhea, slight weight loss and slight depression of red blood cell count without any histological changes (Hodge et al., 1950) Groups of 2 male and 2 female beagle dogs were fed diets containing 0, 2 and 6% low viscosity (10 cP) hydroxypropyl methyl cellulose for 90 days. No evidence of toxicity was observed in dogs as judged by mortality, body weight, food consumption, urine and hematological analyses, serum chemistry, organ weights and gross and histological examination (McCollister et al., 1973). Groups of 4 male and 4 female beagle dogs were fed diets containing 0, 1 and 5% low viscosity (4.22 cP) hydroxypropyl methyl cellulose for 90-91 days. No evidence of toxicity was observed in dogs as judged by mortality, body weight, food consumption, urine, hematology, and serum chemistry analyses, organ weights and gross and histological examination (Schwetz et al., 1973). 2.2.3 Long-term/carcinogenicity studies Rats Groups of 50 male and 50 female rats were fed for two years on diets containing 0, 1, 5 and 20% of hydroxypropyl methyl cellulose, type B. There was a slight retardation of body weight gain in the male group at the highest dose. Mortality ranged from 60 to 84% with no significant difference between the groups. Tumour incidence was the same in the experimental groups as in controls (Hodge et al., 1950). 2.2.4 Reproduction studies No data available. 2.3 Observations in man Twenty-five young adults ingested doses ranging from 0.6 to 8.9 g of hydroxypropyl methyl cellulose, type B, on three separate occasions. Only a mild laxative or constipating effect was noted in several cases. About 97% of the dose, determined as methoxy groups, was recovered from feces (Knight et al., 1952). METHYL CELLULOSE 2.1 Biochemical aspects 2.1.1 Absorption, distribution and excretion Methyl cellulose is usually resistant to microbial attack (Bargen, 1949). In rats it was not hydrolyzed to cellulose and methanol in the intestinal tract and it did not appear to be absorbed (Bauer & Lehman, 1951). Investigations on two male adults and one 10-year-old girl showed that methyl cellulose passed through the digestive tract practically unaltered. When 5-10 g of methyl cellulose were ingested the recovery of methoxyl groups from the feces was almost quantitative. Methanol formation after the taking of methyl cellulose was not significantly different from that observed under normal conditions (Machle et al., 1944). It has been stated that methyl cellulose could be partly hydrolyzed in the digestive tract to units of lower molecular weight, as indicated by a decrease in viscosity. Such intermediate products obtained after acid hydrolysis were fed to mice at a dose of 1 g daily for 28 days without any demonstrable effect on the growth rate (Letzig, 1943). There is some evidence for the excretion of methyl cellulose into the milk of pregnant rats and this causes transient anemia in suckling rats (Baldini, 1958). In rats, a one-time dose of 500 mg/kg bw of [14C]methyl cellulose (labelled in the methoxyl group, and with a viscosity of 3300 cP) was excreted in its entirety (102.2%) in the feces within 48 hours. No radioactivity was detected in the expired air and less than 0.1% of the original dose of radioactivity was found in the urine, selected tissues and remaining carcass. In rats receiving multiple doses of labeled methyl cellulose for 5 days, no accumulation of 14C activity was observed in the body or in selected tissues (Braun et al., 1974). When sucrose-based diets containing 8% methyl cellulose of either low (25 cP), medium (400 cP) or high (1500 cP) viscosity was fed to male rats (Hooded Wistar strain) for 10-11 days, neither food intake nor growth were affected by consumption of methyl cellulose. Tritium incorporation from i.p.-injected tritiated water into fatty acids and cholesterol in the liver was decreased. The authors concluded that the decreased hepatic fatty acid synthesis, decreased serum glucose and increased glycogen storage observed in treated rats could be explained by a slower absorption rate with increasing viscosity of the methyl cellulose diets. No effect of methyl cellulose was noted on cholesterol metabolism, and no evidence was found to suggest that methyl cellulose is fermented by gut microbial flora in the rat (Topping et al., 1988). 2.2 Toxicological studies 2.2.1 Acute toxicity LD50 values for methyl cellulose have not been found in the literature. In dogs, single intravenous injections of 40 ml of 0.7 to 2.8% solutions of methyl cellulose in saline resulted, within 24 hours, in a moderate anemia and leukopenia and an increased sedimentation rate (Hueper, 1944). In rabbits, intravenous injections of 10 to 100 mg methyl cellulose/kg bw in a 1% solution had no effect on blood pressure or respiration (Wiedersheim et al., 1953). In man, single oral doses of 5 and 10 g of methyl cellulose were well tolerated (Machle et al., 1944). Intravenous injection of a 1% solution of methyl cellulose in rabbits induced subintimal deposits of methyl cellulose at arterial walls followed by extensive calcification, ossification, cartilage formation and lipid deposition (Stehbens & Silver, 1966). 2.2.2 Short-term studies 2.2.2.1 Rats A group of 10 rats (5 male and 5 female) was fed a diet containing 10% methyl cellulose for 95 days. The male rats gained weight at the same rate as the controls. The females showed lower food intake and slight growth depression. No abnormalities were found at autopsy or on microscopic examination. Weights of the heart, liver, spleen and kidney were normal. The stomachs were 15% heavier in the experimental group than in the controls (Tainter, 1943). Eighty rats received methyl cellulose at the level of 0.8% in the diet and 1% in the drinking-water for 8 months. This was equivalent to an average total daily intake of 436 mg of methyl cellulose per animal. No effect on growth rate was observed in any of the animals. Water and food intake were normal. No gross or microscopic pathological changes were found post mortem (Deichmann & Witherup, 1943). Groups of 5 female rats were fed diets containing 1.66% and 5% methyl cellulose for six months without any adverse effects (Bauer et al., 1944). Three groups of 10 rats (5 male and 5 female) were given diets containing 0.17% (changed after six weeks to 0.5%) and 5% methyl cellulose for eight months. No deleterious effect on growth was recorded, and macroscopic and microscopic examination of representative animals revealed essentially normal tissues. Deposition of abnormal material in the tissues was not observed. Reproduction was unimpaired through three generations. Second and third generation rats fed a diet containing 5% methyl cellulose for 4 months responded normally (Bauer & Lehman, 1951). A modified paired feeding experiment was conducted on three groups of rats for 90 days; one group received a diet containing 50% methyl cellulose, one a diet containing 50% cellulose powder, and one the basal diet. Growth depression was seen in the first two groups. Subsequent replacement of the methyl cellulose or cellulose diet by the basal diet resulted in marked weight gain (Bauer & Lehman, 1951). In 28-day experiments with groups of 10 rats, some normal and others vitamin-depleted, the oral administration of 50 mg of methyl cellulose did not affect the absorption of either 6 µg of thiamine or 3 units of vitamin A per day, as determined by weight gain (Ellingson & Massengale, 1952). Four intraperitoneal injections over 10 days of a maximal total dose of 160 mg of methyl cellulose produced arterial hypertension and glumerulo-nephritis in rats given a 1% NaCl solution to drink. In a further experiment on rats, methyl cellulose was shown to deposit in the renal glomeruli, leading to reduction of filtration and sodium accumulation if the latter is given as well. Hypertension and glomerular lesions developed (Hall & Hall, 1962). Intravenous injections of 1% methyl cellulose were given to rats at three-day intervals, which produced splenic enlargement 21 days after the last injection. Survival time studies on red cells showed that the enlarged spleens destroyed red cells more quickly (Fitch et al., 1962). Intraperitoneal injections of 2.5% methyl cellulose solution twice weekly into adult rats for one to 16 weeks reduced hematocrits and increased spleen weights in a dose-dependent manner. Foam histiocytes accumulated in the spleen pulp and sinusoids. Electron microscopic observations suggested lysosomal ingestion with phagolysosome formation (Lawson & Smith, 1968). Groups of 10 male and 10 female Sprague-Dawley (Spartan strain) rats were fed for 90 days on diets containing methyl cellulose with a viscosity of 10 cP (0, 1, 3 and 10%) or a viscosity of 4000 cP (0, 3 and 10%). Male rats consuming 10% methyl cellulose (viscosity of 10 cP) exhibited slight reductions in terminal body weight relative to controls, but growth was normal in all other 10 cP treatment groups, and in all rats consuming the 4000 cP methyl cellulose. Food consumption was significantly elevated (up to 10%) at certain treatment levels in rats consuming either of the 2 methyl cellulose preparations as described in the following table: Food Consumption Viscosity Dose Males Females 10 cP 3% N N 10% I N 4000 cP 3% I N 10% I I I = significant increase relative to controls N = no significant change relative to controls No significant treatment-related effect was observed on other toxicological parameters examined in the study, including serum chemistry, hematology, urinalyses, organ weights and pathology (gross and microscopic). No accumulation of test material in the reticuloendothelial system could be detected by histopathologic examination (McCollister, et al., 1973). 2.2.2.2 Dogs Two dogs were fed doses of methyl cellulose increasing from 2 to 100 g daily for one month without any noticeable effect (Bauer, 1945). Solutions containing 0.7 to 2.8% methyl cellulose of different molecular weights in 1% NaCl were administered by i.v. injection to 18 dogs in doses of 40 to 130 ml for 5 days a week. The maximum total dose of 5720 ml was injected within six months. Most of the animals died. Hematological reactions and the formation of foam cells were observed (Hueper, 1944). 2.2.3 Long-term/carcinogenicity studies 2.2.3.1 Rats Groups of 30 Sprague-Dawley rats (Spartan strain) of each sex were fed diets containing 0, 1% or 5% methyl cellulose of viscosity 15, 400 or 4000 cP for 2 yr. Gross pathological examinations were conducted on terminally ill rats or rats dying during the study, and on remaining survivors at termination. During necropsy, sections of grossly visible nodules or masses were preserved for histopathological examination. There was no indication of increased tumour incidence in rats receiving the methyl cellulose diets (McCollister et al., 1973). Groups of 20 Sprague-Dawley rats (Spartan strain) of each sex were fed chow diets containing 0, 1% or 5% methyl cellulose of viscosity 15, 400 or 4000 cP for 2 yr. At termination, gross pathologic examinations were performed and blood was sampled (5 rats from each sex) for hematologic evaluation (PCV, Hb, and WBC counts) and serum chemistry (BUN and AP). Additional groups of rats fed the same diets were interim sacrificed at 12 and 18 mos. and subjected to either gross pathologic examination (10 rats of each sex) or tested for hematology and serum chemistry parameters. During necropsy, selected tissues (lungs, heart, liver, kidneys, spleen, and testes) were weighed and, with pancreas and adrenals, preserved for histopathological evaluation. No treatment-related effect was reported on mortality or any other test parameters over the course of the study (McCollister et al., 1973). Subcutaneous implantation of 500 mg of methyl cellulose as a powder in 25 rats failed to demonstrate carcinogenic properties (Hueper, 1959). 2.2.4 Reproduction studies No data available. 2.2.5 Special studies on teratogenicity 2.2.5.1 Mice Groups of 12-17 pregnant mice were administered daily gavage doses of a methyl cellulose suspension in corn oil (70, 153, 330 and 700 mg/kg bw/day) during days 6-15 of pregnancy. Control groups received daily doses of corn oil (negative control) or acetylsalicylic acid (110 mg/kg bw, positive control) during the same period of pregnancy. On day 17 of pregnancy, dams were subjected to Cesarian section and examination of uterine contents. No dose-related effects on growth, mortality or incidence of gross lesions were observed in treated dams. However, significant reductions in the number of implantations, live fetuses, and corpora lutea were observed in high dose dams, relative to corn oil controls. No increase in incidence of external, visceral and skeletal abnormalities, reduced weight or mortality was observed in fetuses from treated dams (Cannon Labs, 1975). Groups of 20-22 pregnant mice were administered daily gavage doses of a methyl cellulose suspension in corn oil (0, 16, 74, 345, 1600 mg/kg bw/day) during days 6-15 of pregnancy. Control groups received daily doses of corn oil (negative control) or aspirin (150 mg/kg bw, positive control) during the same period of pregnancy. On day 17 of pregnancy, dams were subjected to Cesarian section and examination of uterine contents. Methyl cellulose administered at levels up to 345 mg/kg bw/day had no effect on nidation or on maternal or fetal survival. However, a significant increase in mortality was observed in high dose dams, with a reduced rate of pregnancy in survivors. At term, resorption sites were markedly increased in number, and live fetuses were significantly reduced in number and retarded in maturation and in weight. No evidence of teratogenic effects was seen in fetuses from high dose dams or from dams receiving lower levels of treatment (Food and Drug Research Laboratories, Inc., 1973). 2.2.5.2 Rats Groups of 13-18 pregnant rats were administered daily gavage doses of a methyl cellulose suspension in corn oil (120, 260, 550 and 1200 mg/kg bw/day) during days 6-15 of pregnancy. Control groups received daily doses of corn oil (negative control) or acetylsalicylic acid (250 mg/kg bw, positive control) during the same period of pregnancy. On day 20 of pregnancy, dams were subjected to Cesarian section and examination of uterine contents. No dose-related effects on growth, mortality or incidence of gross lesions were observed in treated dams. The incidences of implantations, live fetuses, corpora lutea, dead fetuses and resorptions in treated dams were within the normal range. No increase in incidence of external, visceral and skeletal abnormalities was observed in fetuses from treated dams, with the exception of an increased incidence of extra centers of ossification in vertebrae of fetuses from high dose dams. Fetal weights were not affected by treatment (Cannon Labs, 1977). Groups of 20-25 pregnant rats were administered daily gavage doses of a methyl cellulose suspension in corn oil (13, 51, 285, 1320 mg/kg bw/day) during days 6-15 of pregnancy. Control groups received daily doses of corn oil (negative control) or aspirin (150 mg/kg bw, positive control) during the same period of pregnancy. On day 20 of pregnancy, dams were subjected to Cesarian section and examination of uterine contents. No dose-related effects on growth, mortality or incidence of gross lesions were observed in treated dams. The incidences of implantations, live fetuses, dead fetuses and resorptions in treated dams were within the normal range. No increase in incidence of external, visceral and skeletal abnormalities was observed in fetuses from treated dams, with the exception of an increased incidence of extra centers of ossification in vertebrae of fetuses from high dose dams, nor were fetal weights affected by treatment (Food and Drug Research Laboratories, Inc., 1973). 2.2.5.3 Hamsters Groups of 22-24 pregnant hamsters were administered daily gavage doses of a methyl cellulose suspension in corn oil (10, 46, 216, 1000 mg/kg bw) during days 6-10 of pregnancy. Control groups received daily doses of corn oil (negative control) or aspirin (250 mg/kg bw, positive control) during the same period of pregnancy. On day 24 of pregnancy, dams were subjected to Cesarian section and examination of uterine contents. No dose-related effects on growth, mortality or incidence of gross lesions were observed in treated dams. The incidence of implantations, live fetuses, dead fetuses and resorptions in treated dams was within the normal range. No increase in incidence of external, visceral and skeletal abnormalities was observed in fetuses from treated dams, nor were fetal weights affected by treatment (Food and Drug Research Laboratories, Inc., 1973). 2.2.5.4 Rabbits Groups of 10-17 pregnant rabbits were administered daily gavage doses of a methyl cellulose suspension in corn oil (7, 32, 148, 685 mg/kg bw) during days 6-18 of pregnancy. Control groups received daily doses of corn oil (negative control) or 6-aminonicotinamide (7 mg/kg bw, positive control) during the same period of pregnancy. On day 29 of pregnancy, dams were subjected to Cesarian section and examination of uterine contents. Dams treated at the highest dose level experienced increased mortality and a decrease in pregnancy rate in survivors, but no dose-related effects on growth or incidence of gross lesions were observed. The incidence of corpora lutea, implantations, live fetuses, dead fetuses and resorptions in treated dams was within the normal range. No increase in incidence of external, visceral and skeletal abnormalities was observed in fetuses from treated dams, nor were fetal weights affected by treatment (Food and Drug Research Laboratories, Inc., 1973). 2.2.5 Special studies on genotoxicity Results of genotoxicity assays on methyl cellulose Concentration of Test System Test Object Methyl cellulose Results Reference Ames test S.typhimurium 50 µg/plate Negative Blevins & (1) TA98, TA100 Taylor, 1982 TA1535, TA1537, TA1538 Ames test S.typhimurium <70 mg/plate Negative Ishidate, Jr., (1) TA92, TA94, et al., TA98, TA100, 1984 TA1535, TA1537, TA1538 Host-mediated S.typhymurium 1 x 4.75, 47.5 Negative Litton assay in rats/ TA1530. G46 & 475 mg/kg bw Bionetics, reverse mutagenesis & 5 x 5000 mg/kg 1974 bw/day Host-mediated S.cerevisiae 1 x 4.75, 47.5 Negative Litton assay in rats/ TA1530, G46 & 475 mg/kg bw Bionetics mitotic & 5 x 5000 mg/kg 1974 re-combination bw/day Chromosomal Chinese hamster <4.0 mg/ml Negative Ishidate, Jr., Aberration fibroblast et al., in vitro 1984 Concentration of Test System Test Object Methyl cellulose Results Reference Chromosome Human embryonic 80, 800 & 8000 Negative Litton Aberration lung cells µg/ml Bionetics Induction (WI-38) 1974 in vitro Chromosome Rat Bone 1 x 4.75, 47.5 Negative Litton Aberration Marrow cells 475 mg/kg bw Bionetics Induction in vivo & 5 x 5000 mg/kg 1974 bw/day Dominant Male Rats 1 x 4.75, 47.5 Negative Litton Lethal Assay 475 mg/kg bw Bionetics & 5 x 5000 mg/kg 1974 bw/day (1) Both with and without rat liver S-9 fraction. 2.3 Observations in man In 3 healthy adults, 5 g of methyl cellulose given twice a day for 8 days approximately doubled the volume of the stools and increased their frequency slightly (Tainter, 1943). There was no evidence of toxicity when 1-6 g of methyl cellulose were taken daily as a laxative for 4 to 240 days (maximum 6 g for 240 days) by 37 patients (Schweig, 1948). In another study, doses of 2.5 to 5.25 g of methyl cellulose taken orally as a gel in 250 ml of water were mildly constipating (Bauer, 1945). Patients have been given 2 g of methyl cellulose before meals without toxic reactions (Bargen, 1949). Two patients given 60-90 ml of a methyl cellulose preparation daily for 5 days developed generalized edema, visual disturbances and neurological signs which disappeared within 72 hours of cessation of intake. Symptoms were paralleled by sodium and water retention, increased serum osmolality and reduced urinary aldosterone excretion (Crane et al., 1969). Five adult male volunteers were given 250 mg methyl cellulose/kg bw daily, divided into 3 equal portions, over a period of 23 consecutive days. The treatment was well tolerated, producing no allergic responses or alteration in normal elimination patterns. Methyl cellulose, administered as a prehydrated gel, caused increased fecal weight (wet and dry basis), but had variable effects on intestinal transit time, causing increased transit time in three subjects and decreased transit time in the other two. Hematology, serum biochemistry and urinalysis parameters remained within normal limits. Small, but significant, reductions were observed in fecal volatile fatty acids and neutral sterols, but breath hydrogen levels were not affected (Eastwood et al., 1988). Fifty healthy adults (44 women and 6 men, 18-70 yr of age) were administered daily doses of bulk laxative containing either a placebo or methyl cellulose (4000 cP, 30% substitution, 2 or 4 g) for a 1 wk period. All subjects had received placebo for the week preceding treatment. In a second phase of the study, 59 adults (56 women, 3 men) suffering from constipation were administered daily doses of either methyl cellulose (1, 2, or 4 g) or 3.4 g psyllium as a positive control for a 1 wk period. All stools generated during the study were weighed and analyzed for solids and moisture content. Stool moisture content and frequency of bowel movements were increased in normal adults consuming 4 g of methyl cellulose, but they were unaffected at the 2 g level of exposure relative to the placebo period of the study. Stool size was unchanged, and no significant effect on stool consistency was noted by the patients. Constipated adults experienced an increase in stool frequency with the psyllium control and with methyl cellulose at all dose levels. No significant increase in incidence of abdominal discomfort or flatulence was reported (Hamilton et al., 1988). METHYL ETHYL CELLULOSE 2.1 Biochemical aspects 2.1.1 Absorption, distribution and excretion After feeding a single dose of 0.6 g of methyl ethyl cellulose in the diet of rats some 90% of the dose was recovered from the feces by the end of the fourth day. Nearly all alkoxyl groups remained attached to the cellulose chain during passage through the gut (Gage, 1962). 2.2 Toxicological studies 2.2.1 Acute studies No data available. 2.2.2 Short-term studies 2.2.2.1 Chicken Day-old Arbor Acres chicks were apportioned into groups of 10 and fed low-fat, high-fat or high-protein diets containing 2% methyl ethyl cellulose or one of a variety of vegetable gums or other polysaccharides for 3 wks. Cellulose was used in the control diet. Chicks grew normally on the methyl ethyl cellulose diet. Feed intake was enhanced by 6% and nitrogen retention was increased by 14%, but fat absorption was not significantly changed relative to controls (Kratzer, et al., 1967). 2.2.3 Long-term/carcinogenicity studies 2.2.3.1 Mice Groups of 50 male and 50 female mice were fed 0, 0.1 and 1% of methyl ethyl cellulose for two years. Body weight was slightly reduced in both sexes at the 1% level in the latter part of the test period. There was no difference between the groups in survival, tumour incidence, blood picture and gross and microscopic appearance of internal organs (Imperial Chemical Industries, 1966). 2.2.3.2 Rats Groups of 50 male and 50 female rats were fed 0, 0.1 and 1% of methyl ethyl cellulose for 2 years. Body weight was reduced in males at the 1% level in the latter part of the test period. There was no difference between the groups in survival, tumour incidence, blood picture and gross and microscopic appearance of internal organs (Imperial Chemical Industries, 1966). 2.2.4 Reproduction studies No data available. SODIUM CARBOXYMETHYL CELLULOSE 2.1 Biochemical aspects 2.1.1 Absorption, distribution and excretion Sodium carboxymethyl cellulose (CMC) is readily hydrolyzed by micro-organisms (Reese et al., 1950). Diastase and cellulases are stated to bring about the breakdown of this compound (Letzig, 1943). Pepsin and pancreatin, separately or in combination, do not attack the substance (Massatsch & Steudel, 1941). In 5 rats fed 5 g of CMC collectively, approximately 90% of the dose was recovered in the feces (Shelanski & Clark, 1948). Experiments on 6 rats during 4 periods of 10 days each showed that CMC given in the diet at levels of 5%, 10% and 14% was reclaimed quantitatively in the feces (Ziegelmayer et al., 1951). [14C]-labelled CMC, containing up to 0.34% radioactive sodium glycolate, was given orally to 2 groups of 5 male rats each in a dose of 400 mg. No detectable activity (less than 0.02% of the dose) was found in the livers and kidneys and about 0.14% of the administered radioactivity was found in the 48-hour urine samples. This amount, however, could be accounted for by the free radioactive glycolate present in the test compound (Wiebe et al., 1962). Only about 50% of the intake of CMC could be recovered from the feces of two rabbits on diets containing 4.76% and 9% CMC. Two dogs received 10 g of CMC for one day and 20 g daily for the following 5 days. The total doses were recovered quantitatively in the feces (Ziegelmayer et al., 1951). Two human adults were given 30 g of CMC by mouth daily for 4 days and a third was given 20 g/day. About 90% of the compound was recovered from the feces (Ziegelmayer et al., 1951). 2.2 Toxicological studies 2.2.1 Acute toxicity Species Route LD50(mg/kg bw) Reference Rat oral 15,000-27,000 Shelanski & Clark, 1948 CTFA, 1977 CTFA, 1978a,b CTFA, 1980 Guinea-pig oral 16,000 Shelanski & Clark, 1948 Rats, guinea-pigs and rabbits showed no symptoms after administration by stomach tube of 300 mg/kg bw in three divided doses (Rowe et al., 1944). Six rats given an intravenous injection of 1 ml of a 1.6% solution of CMC showed the presence of particles localized in cells of the reticulo-endothelial system 48 hours later (Jasmin & Bois, 1961). Four dogs given an i.v. injection of 40 ml of 0.25% CMC in 1% sodium chloride solution reacted with a transitory leukopenia (Hueper, 1945). 2.2.2 Short-term studies 2.2.2.1 Rats Ten rats received 300 to 500 mg of CMC daily for two months without any adverse effect (Werle, 1941). Ten male and 15 female rats were fed a diet containing 5% CMC for 201 to 250 days. Judged by growth rate, mortality, organ weights and the results of histopathological examination of the liver, kidney, spleen, pancreas, adrenal gland, testis and gastrointestinal tract, there were no significant differences between the treated and the control groups (Rowe et al., 1944). Another group of 10 rats received a diet containing 20% of CMC for 63 days. Slight growth retardation and a laxative effect were observed. Organ weights and both gross and microscopic pathological examination revealed no abnormalities (Rowe et al., 1944). Two groups of 100 rats received 500 and 1000 mg/kg bw/day of CMC mixed in their diets for six months. No adverse effects were observed in any of the experimental animals as determined by growth rate, fertility and examination of the blood, urine and main tissues (Shelanski & Clark, 1948). Six rats were fed 14% of CMC in the diet for five weeks without demonstrable deleterious effect (Ziegelmayer et al., 1951). Ten rats given subcutaneous injections of CMC showed mast-cell- like elements within the adrenal medulla. Changes in the adrenocortical cells and the presence of granules in the adrenal-vein were noted occasionally (Selye, 1955). Rodents (12 animals per group) were maintained for 21 days on a high-protein diet containing 0 or 15% sodium CMC of 10 viscosity grades (35-4500 cP) or 4 other vegetable gums. Animals were weighed on alternate days. Body weight gain for one sample of CMC exceeded that of controls and body weight gains for two CMC samples were less than that of controls. Average fecal water content (measured as %) was increased in all CMC-fed animals from 1.9-3.0 fold, and average filled cecal weight (g/kg bw) was increased 1.5-3.3 fold relative to controls. It was noted that there was a tendency for CMC samples of low molecular weight to produce high fecal wet weights. Measuring the viscosities of completely hydrated samples of CMC indicated that CMC can have large or narrow molecular weight distributions. It was suggested that different molecular weight distributions in samples of CMC may produce different physiologic or dietary responses to CMC (Anderson, 1986). 2.2.2.2 Guinea-pigs Two groups of 100 animals were fed CMC for six months at levels of 500 and 1000 mg/kg bw/day mixed in the diet. No signs of toxicity were observed. Two groups of 20 guinea-pigs received CMC in their diet at rates of 500 and 1000 mg/kg bw/day for one year. As judged by weight gain, gross and histopathological examination, no adverse effects were noted (Shelanski & Clark, 1948). 2.2.2.3 Rabbits Three rabbits were fed CMC at levels of 4.8% and 9% in their diet for two periods of 15 days without any detectable toxic effects (Ziegelmayer et al., 1951). 2.2.2.4 Dogs Two dogs were given daily doses of CMC (0.3-0.4 g/kg bw) in water by mouth for two months without adverse effects (Werle, 1941). Groups of 10 dogs were fed CMC in the diet at levels of 500 and 1000 mg/kg bw/day for six months. Growth rate was the same in all groups. Six animals from each group were examined post mortem. Histologically, the stomach, intestines, spleen, kidney, heart, lung and pancreas in treated animals were no different from those of controls (Shelanski & Clark, 1948). Five dogs received intravenous injections of 0.25% CMC in 1% sodium chloride solution in doses increasing from 40 ml to 150 ml for a maximum of three months. There were no gross pathological changes. Histopathological studies revealed uptake of CMC in the reticuloendothelial cells in the aorta (Hueper, 1945). 2.2.2.5 Chickens Groups of 20 one-day-old chicks were maintained on diets containing 0 or 2% sodium CMC for 20 days. Addition of sodium CMC to the diet resulted in decreased growth rate (Vohra & Kratzer, 1964). 2.2.3 Long-term/carcinogenicity studies 2.2.3.1 Mice Groups of 50 male and 50 female mice were maintained for up to 100 weeks on ad libitum diets containing 0, 0.1 and 1% of sodium CMC. There was no apparent difference in mortality and tumour incidence between the groups (Imperial Chemical Industries, 1966). Groups of 50 male and 50 female B6C3F1 mice were used as vehicle- controls in a carcinogenicity study of selenium sulfide, and received 50 mg/kg bw of CMC by gavage, 5 days per week for 103 weeks. Untreated mice served as controls. Test animals were observed twice daily and examined weekly for clinical signs and the presence of palpable lesions. Mean body weights were recorded every two weeks for the first 12 weeks, then monthly for the remaining 93 weeks. Animals that were moribund and those that survived to the end of the study were necropsied. Gross and microscopic examinations were performed on major organs and all gross lesions. CMC-gavaged animals had approximately the same or fewer neoplasms than untreated control animals (NCI, 1979). 2.2.3.2 Rats Groups of 25 rats, divided about equally by sex, were placed for two years on diets containing CMC in concentrations providing 100, 500 and 1000 mg/kg bw daily. Three generations of litters were produced and kept on the same diet as their parents. According to growth rates, monthly urine and blood examinations, fertility, and histopathological examination of the main organs there were no differences between the test rats and the controls. No neoplasms were found in any of the experimental animals (Shelanski & Clark, 1948). Groups of 50 male and 50 female rats were maintained for up to two years on ad libitum diets containing 0, 0.1 and 1% of sodium CMC. No difference in mortality and tumour incidence was apparent between the groups (McElligot & Hurst, 1968). Thirty rats were given weekly injections of 1 ml of a 2% aqueous solution of CMC subcutaneously. After 73 weeks, 43% of the animals showed tumour s at the site of injection, characterized as fibrosarcomas of moderate malignancy (Lusky & Nelson, 1957). Twenty rats were given subcutaneous injections once a week of 2% aqueous solution of CMC. In 4 animals, tumours developed at the site of injection within 13 to 16 months. Two of the neoplasms were fibromas and two fibrosarcomas (Jasmin, 1961). Fifty F344 rats of each sex served as the vehicle control for a carcinogenicity study of selenium sulfide, and received 5 mg/kg bw of CMC by gavage five days per week for 103 weeks. Untreated rats served as controls. Test animals were observed twice daily and examined weekly for clinical signs and the presence of palpable lesions. Mean body weights were recorded every two weeks for the first 12 weeks, then monthly for the remaining 93 weeks. Animals that were moribund and those that survived to the end of the study were necropsied. Gross and microscopic examinations were performed on major organs and all gross lesions. Eighty percent of CMC-fed male rats and 76% of CMC-fed female rats survived until the end of the study. These percentages were similar to those for untreated rats. CMC-gavaged animals had approximately the same or fewer neoplasms than untreated control animals (NCI, 1979). 2.2.4 Reproduction studies No data available. 2.2.5 Special studies on cecal microflora Groups of 6 male Sprague Dawley rats with conventional gut microflora were fed a purified diet containing 0 or 5% of CMC for 4 weeks. After 4 weeks, the rats were killed by cervical dislocation and the ceca removed; cecal contents from each animal were removed and maintained under anaerobic conditions for analysis of gut microflora and measurement of enzyme activities. CMC decreased the final body weight of the rats by 9% compared to the control group. The weight of the cecal wall was significantly increased as was the weight of cecal contents. Feeding CMC to rats significantly increased the total bacterial population of the cecum. No attempt was made to analyze the species composition of the microflora. Feeding CMC to rats also significantly increased in vitro activity of bacterial azoreductase, beta-glucosidase, beta-glucuronidase, nitrate reductase, and urease. The greatest effect on for urease activity (elevated 5.3 fold) and beta-glucosidase activity (elevated 8.2 fold). The authors suggested that the fact that feeding CMC increased the total activity of all enzymes studied may indicate that the beta-1,4-glucose backbone of CMC was susceptible to attack by the intestinal bacteria (Mallett et al., 1984). Groups of 5 male Wistar rats were fed a fiber-free synthetic diet containing 0 or 10% CMC, hydroxypropyl methyl cellulose, or carboxymethyl guar for 12 days. At termination, rats were anesthetized and killed by cervical dislocation; cecum and ascending colon were removed. All diets supplemented with non-digestible polysaccharides led to a visible enlargement of the cecum, which was associated with increased wet weight of contents and of tissue. The enlargement in the CMC-supplemented group was particularly marked, with an almost 8-fold increase in weight of cecal contents and a doubling of tissue weight. Animals fed the CMC-supplemented diet had diarrhea (profuse unformed stools) from the 2nd day of feeding, and the total output of fecal material was significantly increased. CMC did not significantly increase the density of bacteria in the cecum and colon, but because of the increased cecal mass the absolute number of bacteria per cecum was elevated. The aerobic flora of the fiber-free controls was composed primarily of Streptococcus spp., but aerobic bacteria found in cecal and colonic contents of rats fed CMC were almost entirely E.coli. No enterotoxin activity was detected in the E.coli strains isolated from CMC-fed rats with diarrhoea, and serotyping showed that the strains belonged to groups that are not commonly associated with diarrhea in man. A substantial amount of unfermented CMC was present in the intestinal contents of rats fed the CMC-supplemented diet, and the proportion of CMC increased distally. The CMC appeared not to have been hydrolyzed to shorter chain lengths, as no increase in reducing ends could be demonstrated compared with animals maintained on the control diet. CMC also was poorly fermented by cecal and colonic bacteria in vitro. In addition, rats fed the CMC-supplemented diet had significantly lower short-chain fatty acid concentrations in the cecum. The authors concluded that enlargement of the rat cecum in response to dietary non-digestible polysaccharides such as CMC can occur when the polysaccharide is resistant to fermentation (Wyatt et al., 1988). 2.2.6 Special studies on genotoxicity Results of genotoxicity assays on CMC Concentration Test System Test Object of CMC Results Reference Ames test S.typhimurium 5.0% Na CMC Negative Litton (1,3) TA1535,TA1537 Bionetics TA1538 1975 Ames test S.typhimutrium 2.5, 5.0 & 10% Negative Litton (l,4) TA1535,TA1537 Na CMC Bionetics TA1538 1975 Ames test S.typhimurium 0.5, 1, 10, 100, Negative Litton (2,3) TA98,TA100, 1000, 2500 & 5000 Bionetics TA1535,TA1537 µg Na CMC/plate 1980 TA1538 Ames test S.typhimurium <2.5 mg Na Negative Ishidate, (2,3) TA92,TA94,TA98, Na CMC Jr. et al. TA100,TA1535, 1984 TA1537 Recombinogenicity S.cerevisiae 0.25, 0.50, 1.00% Negative Litton D4 - ade Na CMC Bionetics D4 - try 1975 Concentration Test System Test Object of CMC Results Reference Chromosome Chinese Hamster <2.8 mg Na Negative Ishidate, Aberration fibroblasts Na CMC/ml Jr. et al. 1984 (1) Both with and without rat, mouse or monkey liver, lung or testes S-9 fraction (2) Both with and without rat liver S-9 fraction (3) Plate incorporation assay (4) Suspension assay 2.2.7 Special studies on teratogenicity 2.2.7.1 Mice Sodium CMC (0, 16, 74, 345, 1600 mg/kg bw/day) was administered as a corn oil solution by gavage to groups of 19-24 pregnant mice (Albino CD-1 outbred females) from days 6-15 of gestation. A positive control group of 24 pregnant mice received 150 mg aspirin/kg bw/day. All pregnant females survived until the end of the study. No effects were observed on nidation or on maternal or fetal survival. The number of abnormalities seen in either soft or skeletal tissues of the test groups did not differ from the number occurring spontaneously in sham-treated controls (Food and Drug Research Laboratories, 1975). 2.2.7.2 Rats Sodium CMC (0, 16, 74, 345, 1600 mg/kg bw/day) was administered as a corn oil solution by gavage to groups of 19-22 pregnant rats (Wistar-derived) from days 6-15 of gestation. Nineteen pregnant rats (positive control group) were dosed with 250 mg aspirin/kg bw/day. All pregnant females survived until the end of the study. No effects were observed on nidation or on maternal or fetal survival. The number of abnormalities seen in either soft or skeletal tissues of the test groups did not differ from the number occurring spontaneously in sham-treated controls (Food and Drug Research Laboratories, 1975). Twenty male rats (albino, Sprague-Dawley-derived) were treated at least 60 days and 40 female rats were treated at least 14 days before mating and during a 6-day mating period with 200 mg/kg bw/day by gavage; 20 male and 40 female rats were maintained under identical conditions but were not dosed with CMC (controls) For one half of the females, treatment was continued until sacrifice on day 14 of gestation; for the remaining half of the females, treatment continued until weaning of the progeny (day 28 after birth). No reactions to treatment with CMC were noted in the parents. Average body weights were comparable throughout the experiment (however, the body weight gain for males treated with CMC was less than the body weight gain for control males at 7 of the 10 weighings). No difference was observed in mating efficiency or pregnancy rate. The mean numbers of corpora lutea and implantation sites, as well as the ratios of corpora lutea to implantation sites, were comparable in both the CMC-treated and control groups. The rate of resorptions was not significantly increased in the CMC-treated group. In both groups of rats, the normal pregnancy duration was maintained (21-22 days) and no disturbances of parturition were noted. No significant difference was noted in litter size and sex ratio between the CMC-treated and control groups. The body weight gain of the pups was comparable for both groups. No abnormal nesting behavior (nursing, suckling, and creeping) was noted, and eye opening and pinna detachment followed the normal course in both groups. Results of behavioral tests (which included righting reflex, photophobotaxis, cliff avoidance, palmar grasp ability, negative geotaxis, and exploratory locomotion pattern in a cylindrical cage, direct pupillary reflex, and hearing ability by startle response), were comparable in test and control offspring (Fritz & Becker, 1981). 2.3 Observations in man Daily oral doses of 20 to 30 g of Na CMC for seven days were well tolerated by 3 human subjects (Ziegelmayer et al., 1951). Eleven patients received 10 g of Na CMC daily for six months without complaint, but in seven further cases the dose had to be reduced owing to abdominal discomfort. No hematological changes were observed (Brick, 1952). Adult patients have been treated for more than a year with daily oral doses of 2-6 g of CMC as a laxative and there were no ill effects (Brick, 1949; Fittipoldi & Davis, 1948; Schultz, 1949). Skin tests on 100 men and 100 women demonstrated that sodium CMC is not a primary irritant or a sensitizer (Shelanski & Clark, 1948). Twelve men (26-62 years of age; mean age of 38) weighing from 77.5 to 111.5 kg (mean weight 88.7 kg) consumed a basal diet containing four refined fibers (cellulose, CMC, locust bean gum, or karaya gum) at a level of 0.75 g fiber/100 calories (19.1 to 26.0 g/day). Each of the four fibers were fed for 4 weeks in a randomized rotation pattern throughout the study, so that each fiber was followed by every other fiber. The basal diet contained 14.6% of calories as protein, 35% of calories as fat (641 mg cholesterol), and 50.4% of calories as carbohydrate, with a relatively low amount of endogenous fiber. Caloric intake ranged from 2550 to 3600 kcal/day. At the end of each 4-week dietary period, fasting plasma cholesterol (HDL, LDL, and VLDL), serum total cholesterol, triglycerides, and free fatty acids were determined. At the end of 4 weeks, total serum cholesterol had dropped significantly (from 196 mg/dl base line, to 164 mg/dl after CMC) and plasma LDL decreased significantly (from 131 mg/dl to 107 mg/dl). No statistically significant changes were observed in triglycerides, free fatty acids, VLDL, or HDL levels. The HDL/(VLDL+LDL) ratio after consuming CMC-supplemented diets for 4 weeks was significantly higher than the ratios after consumption of the basal diet alone or of the cellulose-supplemented basal diet (Behall et al., 1984). Five healthy men (24-58 yrs of age, weighing 73-84 kg each) consumed 15 g CMC daily (5 g CMC, 3 times a day) for 23 days following a 7-day control period. Results of a diet inquiry for the 1st and 4th weeks of the experiment showed no differences in individual intakes of protein, fat, carbohydrate, sugar, alcohol, and fiber. CMC was well tolerated by all subjects and no adverse effects were observed. CMC had no effect on plasma biochemistry, urinalysis, glucose tolerance, serum cholesterol, triglyceride and phospholipids, breath hydrogen and methane concentrations. The average intestinal transit time decreased. Fecal wet and dry weights increased substantially when CMC was added to the basal diet. Mean fecal bile acid levels and fecal fat content increased. The mean excretion of neutral sterols decreased from 48 to 29 umol sterols/g dry weight of feces (Anderson et al., 1986; Anderson, 1986). 3. COMMENTS Since the previous evaluation, additional data have become available. These data include studies in rats on cecal enlargement and changes in cecal flora, teratology and development, as well as in vitro mutagenicity studies on methyl cellulose and carboxymethyl cellulose. These studies confirmed the conclusion of the earlier meetings of the Committee that modified celluloses have a low toxicity. Long-term/carcinogenicity studies on hydroxypropyl methyl cellulose, methyl cellulose, methyl ethyl cellulose, and sodium carboxymethyl cellulose in rats and mice are available. No evidence of mutagenicity or carcinogenicity has been observed. Reproduction and teratology studies with hydroxypropyl cellulose, methyl cellulose, and sodium carboxymethyl cellulose have been performed in mice, rats and rabbits. The consumption of these modified celluloses did not interfere with the reproductive process, and no embrytoxic or developmental effects were observed. A new substantial body of human data was available investigating the laxative effects of modified celluloses which occurs in some subjects at levels as low as 5 g/person/day. At higher doses diarrhea has been reported in some subjects, but in others constipation developed. Studies in humans did not exceed the addition of 30 g/person/day. An intake of 30 g/day has been recommended as the upper safe level of dietary fiber in general (NRC, 1989). 4. EVALUATION Estimate of acceptable daily intake ADI "not specified". The ability to produce laxation should be taken into account when using these substances as food additives. See p.78 for the definition of "ADI not specified". 5. REFERENCES ANDERSON, D.M.W., EASTWOOD, M.A. & BRYDON, W.G. (1986). The dietary effects of sodium carboxymethylcellulose in man. Food Hydrocolloids 1, 37-44. ANDERSON, D.M.W. (1986). Some dietary effects of the ingestion of sodium carboxymethylcellulose by man and rodents. Unpublished Report from University of Edinburgh. Submitted to WHO by Organisation des Fabricants Cellulosiques Alimentaires (OFCA). BALDINI, M. (1958). Proc. 6th Int. Congr. Int. Soc. Haematol., New York, GR & STR. BARGEN, J.A. (1949). A method of improving function of the bowel: the use of methylcellulose. Gastroenterology 13, 4, 275-278. BAUER, R.O. (1945). Methyl cellulose: its laxative action and effects of chronic feeding on growth and reproduction. Fed. Proc. 4, 112. BAUER, R.O. & LEHMAN, A.J. (1951). Chronic toxicity studies on methyl cellulose in rats. J.Amer.Pharm.Assoc.,Sci.Ed. 40, 257-260. BAUER, R.O., LEHMAN, A.J. & YONKMAN, F.F. (1944). Chronic toxicity of an alkyl ether of cellulose, methyl cellulose. Fed.Proc. 3, 65-66. BEHALL, K.M., LEE, K.H. & MOSER, P.B. (1984). Blood lipids and lipoproteins in adult men fed four refined fibers. Am.J.Clin. Nutrition, 39, 209-214. BLEVINS, R.D. & TAYLOR, D.E. (1982). Mutagenicity screening of twenty-five cosmetic ingredients with the Salmonella/microsome test. J.Environ.Sci. Health, A17, 217-239. BRAUN, W.H., RAMSEY, J.C. & GEHRING, P.J. (1974). The lack of significant absorption of methylcellulose, viscosity 3300 cp. from the gastrointestinal tract following single and multiple oral doses to the rat. Fd.Cosm.Toxicol. 12, 373-376. BRICK, J.B. (1949). 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See Also: Toxicological Abbreviations Modified celluloses (WHO Food Additives Series 5) MODIFIED CELLULOSES (JECFA Evaluation)