FAO Meeting Report No. PL/1965/10/1 WHO/Food Add./27.65 EVALUATION OF THE TOXICITY OF PESTICIDE RESIDUES IN FOOD The content of this document is the result of the deliberations of the Joint Meeting of the FAO Committee on Pesticides in Agriculture and the WHO Expert Committee on Pesticide Residues, which met in Rome, 15-22 March 19651 Food and Agriculture Organization of the United Nations World Health Organization 1965 1 Report of the second joint meeting of the FAO Committee on Pesticides in Agriculture and the WHO Expert Committee on Pesticide Residues, FAO Meeting Report No. PL/1965/10; WHO/Food Add./26.65 ALLETHRIN Chemical name d1-2-allyl-4-hydroxy-3-methyl-2-cyclopenten-1-one esters of cis and trans d1-chrysanthemum monocarboxylic acid. Synonyms allyl homologue of cinerin I (see monograph on pyrethrins) Empirical formula C19H26O3 (Mol. wt. 302.4) Structural formulaRelevant physical and chemical properties Allethrin is a synthetic pyrethroid and has chemical properties similar to those of pyrethrins, however it is more stable on exposure to heat and sunlight. Detoxification occurs by double bond hydrogenation of acid or allyl side chains, and it hydrolyses to chrysanthemic acid and 2-allyl-3-methyl-2, 4-cyclopentadienone. Allylrethrolone and chrysanthemic acid exist as optical isomers. Chrysanthemic acid also exists as geometric isomers. There are eight optical and geometric isomers of allethrin, all possibly present in the technical material. Insecticidal activity depends on the proportions of the various isomers present (Negherbon, 1959). Uses Allethrin, alone or in combination with synergists, is used in many instances where pyrethrins are used for the same purpose. If price is reduced in the future, use may be expanded. Principal formulations are technical allethrin (usually 95%); 1% dust; 0.1% to 0.6% aerosol formulations and in distillate formulations with or without synergists and other ingredients. When used alone it is exempt from tolerances for residues (in Canada and U.S.A.) except for post-harvest application to grain, with no limitations on use. Residue tolerances have been established when used in conjunction with synergists. It is particularly useful as dusts on a wide range of horticultural crops when other pesticides cannot be used close to date of harvest. It is recommended in synergized formulations as aerosols or dips for post-harvest use on fruits and berries, in storage and in processing plants. Agricultural premises, including milk rooms, are treated with allethrin aerosols or surface sprays where residues of other pesticides must be avoided. Direct application is approved for beef and dairy cattle, sheep, goats, poultry and swine. Post-harvest use for stored grain (surface treatment) has also been approved in some countries. Residues Methods described for pyrethrins residue analysis are applicable for allethrin, except that the standard is made from allethrin. A colorimetric method for allethrin residues in milk and meat has been described (McClelland and Moore, 1958). The method is reported accurate to 0.1 ppm or 10 mmg/100g sample. Allethrin was not found in the milk of dairy cows, which had been sprayed daily for three weeks, or in the meat tissue of a female goat that had been sprayed daily for five weeks, all with a large overdose of spray. No information is available on the chemical nature of terminal residues in treated crops. Effect on treated crop There is no information available on the effect of allethrin on treated crops. BIOLOGICAL DATA Acute toxicity Animal Route LD50 mg/kg Reference body-weight Rat, male Oral 920 Carpenter et al., 1950 Rat, female Oral 900 " Mouse, male Oral 480 " 20% in Deo-base Mouse, male, Oral 1580 " 5% in Deo-base Rabbit, male Oral 4290 " Short-term studies Rat. Rats showed a slight decrease in growth-rate when fed commercial allethrin at a dietary level of 5000 ppm and a more nearly normal growth-rate when fed the same concentration of purified allethrin. A dietary level of 2500 ppm apparently produced no clinical effect. No examination of the liver was reported (Ambrose & Robbins, 1951). In another study rats that were fed allethrin for 16 weeks showed no gross effect at 5000 ppm but did show tremor and convulsions at 10 000 ppm (Lehman, 1952). It was shown that rats (and dogs) withstand repeated inhalation of allethrin aerosols at many times the concentration in air that would possibly be reached under practical conditions. The method of dosage did not lend itself to measurement on the basis of milligram per kilogram of body-weight (Carpenter et al., 1950). Dog. Four male and four female dogs fed allethrin at a rate of 50 mg/kg/day for two years showed no gross or microscopical effects. At higher dosage levels in other groups, there were convulsions, progressively shortened survival, and non-specific pathological changes (Lehman, 1965). Man. There are apparently no reports of allergy to allethrin comparable to those associated with pyrethrum and pyrethrins. However, since the types of exposure have not been the same the possibility of allergy to allethrin cannot be excluded. Comments on the experimental studies reported and evaluation Rats apparently tolerate prolonged feeding at a rate of 125 mg/kg/day but the exact duration of the test was not stated. A dosage of 50 mg/kg/day for 2 years produced no detectable effect in dogs. Because no study of the liver seems to have been made in rats and because no study has been made in any species for more than half its life-span, it is not possible to estimate an acceptable daily intake for man. Further work required Long-term studies in at least two species are required in which particular attention is paid to possible effects on the liver. The metabolism of the substance should be explored. REFERENCES Ambrose, A. M. & Robbins, D. J. (1951) Fed. Proc., 10, 276 Carpenter, C. P., Weil, C. S., Pozzani, U. C. & Smyth, J. F., jr (1950) Arch. industr. Hyg., 2, 420 Lehman, A. J. (1952) Quart. Bull. Assoc. Food and Drug Officials U.S., 16, 47 Lehman, A. J. (1965) Summaries of pesticide toxicity (In press) McClellan, D. B. & Moore, J. B. (1958) J. Agr. Food Chem., 6, 463 Negherbon, W. (1959) Handbook of Toxicology, vol. 3, Saunders, Philadelphia
See Also: Toxicological Abbreviations Allethrin (ICSC) Allethrin (UKPID)