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    PESTICIDE RESIDUES IN FOOD - 1983


    Sponsored jointly by FAO and WHO






    EVALUATIONS 1983





    Data and recommendations of the joint meeting
    of the FAO Panel of Experts on Pesticide Residues
    in Food and the Environment and the
    WHO Expert Group on Pesticide Residues
    Geneva, 5 - 14 December 1983

    Food and Agriculture Organization of the United Nations
    Rome 1985


    CYHEXATIN

    RESIDUES

    Explanation

         The 1978 Meeting proposed a maximum residue limit (MRL) of
    2 mg/kg for cyhexatin on peaches.1  At the 15th Session of the Codex
    Committee on Pesticide Residues (CCPR) in 1983 the United States
    referred to agricultural practices in that country that could result
    in residues exceeding 2 mg/kg. New information was reviewed by the
    present Meeting, which indicated a need to increase the MRL.

    RESIDUES IN FOOD AND THEIR EVALUATION

    RESIDUES RESULTING FROM SUPERVISED TRIALS

    Peaches

         Residue studies were conducted at several locations in the United
    States and in Ontario, Canada on seven varieties of peaches.
    Treatments were up to three times with dilute sprays of 20 g a.i./100
    1 applied to 3 400 to 9 000 l/ha or concentrated sprays of 0.84 kg or
    1.68 kg a.i./ha applied at 450 l/ha.

         Sample peaches were harvested at intervals after the last
    application and analysed for total organotin residue, including
    cyhexatin, dicyclohexyltin oxide and cyclohexylstannoic acid. The
    method involved treating macerated fruit with hydrochloric acid,
    extraction with warm chloroform to separate organotin compounds from
    inorganic tin, evaporation of the chloroform, oxidation with nitric
    and sulphuric acids, conversion of the resultant Sn+4 to its iodide,
    formation of pyrocatechol violet complex and spectrophotometric
    measurement of the colour (M&T Chemicals 1972). The residues found are
    summarized in Table 1 (Getzendaner & Carbin 1973, 1975). All are
    expressed as cyhexatin, which predominates at any given time.

    Kiwi Fruit

         Cyhexatin is the preferred miticide for the control of
    phytophagus mites on kiwi fruit and has been used at between 12.5 g
    and 20 g a.i./100 l, with one or two applications per season. The
    volume varied from 1 200 l/ha to 3 400 l/ha, depending on application
    equipment, equivalent to 150 g to 700 g a.i./ha.

         Residue trials carried out in New Zealand in 1978, 1979 and 1981
    are reported in Table 2.

        Table 1   Residues of Cyhexatin in Peaches
                                                                                                        

                                                     Application           AHI1     Total organotin found
    Year      Country             Variety        Rate          No.        (days)    (as mg/kg cyhexatin)
                                                                                                        

    Dilute sprays (20 a.i./100 l, 3 400-9 000 1/ha)

    1969      United States       Halford        1.7            3            0      2.0, 2.5, 2.8, 3.8
                                                                             7      3.2, 3.2, 3.2, 3.6
                                                                            14      2.4, 2.4, 3.2, 3.6
                                                                            28      1.9, 2.0, 2.2, 2.7
                                                                2           53      0.6, 1.2, 1.4, 1.5
                                                                1           53      0.6, 0.9, 1.0, 1.1

    1970                          Halford        1.7            3            0      0.9, 1.5, 1.7, 2.3
                                                                             7      1.1, 1.3, 2.1, 2.2
                                                                            14      2.0, 2.1, 2.4, 2.6
                                                                            28      1.2, 1.4, 1.5, 1.5

    1970                          Halford                       3            0      1.5, 1.7, 1.7, 2.0
                                                                             7      1.2, 1.4, 1.4, 1.5
                                                                            14      0.9, 1.0, 1.1, 1.2
                                                                            28      0.4, 0.5, 0.5, 0.6
                                                                                                        

    1  See Annex 2 for FAO and WHO documentation.

    Table 1 (con't)
                                                                                                        

                                                     Application           AHI1     Total organotin found
    Year      Country             Variety        Rate          No.        (days)    (as mg/kg cyhexatin)
                                                                                                        

    1970      United States       Red Haven      1.8-2.2        3            0      1.6, 1.9, 2.3
                                  (early)
                                                                             7      1.7, 2.0, 2.0
                                                                            14      0.5, 0.6, 0.8
                                                                            28      0.9, 0.9, 1.0

    1970                          Richaven       1.1-1.8        3            0      1.8, 2.0, 2.2, 2.2
                                                                             7      1.8, 2.1, 2.4, 2.5
                                                                             8      0.9, 0.9, 1.3, 1.4
                                                                            20      0.4, 0.6, 0.6, 0.7

    1972      Canada              Goldray        1.1            1            7      0.6, 0.6, 0.8, 1.6
                                                                            17      0.2, 0.4, 0.5, 0.6
                                                                            25      0.2, 0.3, 0.3, 0.5
                                                                            30      0.1, 0.1, 0.1, 0.2
                                                                            60      0.0, 0.0, 0.0, 0.0

    Concentrated sprays (0.84 kg or 1.68 kg a.i./ha in 450 l/ha)

    1971      United States       Elberta        0.84           3            0      0.3, 0.4, 0.5, 0.5

    1974      United States       Elberta        1.68           3            1      3.0, 4.1, 4.3
                                                                            14      1.2, 1.9, 2.6
                                                                                                        

    1  AHI = Interval from last application to harvest.

    Table 2  Residues of Cyhexatin on Kiwi Fruit
                                                                                                            

                             Rate              Formulation                     Residues (mg/kg)
    No.                      (g a.i./ha)                                   after last trial (days)
                                                                1         2         4         7         14
                                                                                                            

    1979
    1                        680                                1.9       -         -         2.4       1.6

    1978
    1                        700                                1.0       -         -         1.4       1.3

    1981

    2 (14-day interval)      600 (total)       W.P.             1.7       -         -         1.4       0.8
    2 (14-day interval)      500 (total)       aqueous susp.              2.1       1.9       1.4       1.6
    2 (14-day interval)      300 (total)       W.P.             -         1.0       0.8       0.5       0.6
    2 (14-day interval)      600 (total)       W.P.             -         1.6       2.4       0.5       0.5
                                                                                                            
    
         In five trials the edible part was also analysed separately and
    was found to contain less than 10 percent of the level in whole fruit,
    except in the trial using an aqueous suspension when the proportion
    was about 15 percent.

         In general, the new data for peaches support those evaluated in
    1978. However, it is clear that at shorter intervals between last
    application and harvest, and particularly if the chemical is applied
    in lower volumes than previously considered, then the earlier estimate
    of 2 mg/kg as MRL will be exceeded. Thus, the Meeting estimated a new
    MRL of 5 mg/kg, to take into account the new data and those in the
    1978 evaluation, noting again that most of the residue was present in
    the peel and that lower residues are found in smooth-skinned
    varieties. The Meeting recommended that this estimate was suitable for
    establishing an MRL.

    APPRAISAL

         Residue data on kiwi fruit from five trials over three seasons
    have provided a basis for estimating an MRL. Levels in the edible part
    of the fruit were up to about 15 percent of the whole-fruit residue.
    Since MRLs are recommended for kiwi fruit on a whole-commodity basis,
    5 mg/kg was proposed on the basis of a 3 to 4 day preharvest interval.

         The Meeting reviewed the limited data on residues of cyhexatin on
    beans in the 1978 evaluations and concluded that an estimated MRL of
    0.2 mg/kg was more appropriate than the figure of 0.5 mg/kg previously
    estimated.

    REFERENCES - RESIDUES

    Getzendaner, M.E. & Carbin, H.B. Residues of organotin on peaches and
    1973      nectarines, fresh and dried plums from applications of
              PLICTRAN 50W miticide. Dow Chemical Company report GH-C 643.
              (Unpublished)

    Getzendaner, M.E. & Carbin, H.B. A study of residues on stone fruit
    1975      from concentrate spray applications of PLICTRAN 50W
              miticide. Dow Chemical Company report GH-C 800.
              (Unpublished)

    M&T Chemicals. Determination of small amounts of organotin in
    1972      macerated fruit. Analytical method TA-27. (Unpublished)


    See Also:
       Toxicological Abbreviations
       Cyhexatin (WHO Pesticide Residues Series 4)
       Cyhexatin (WHO Pesticide Residues Series 5)
       Cyhexatin (Pesticide residues in food: 1978 evaluations)
       Cyhexatin (Pesticide residues in food: 1980 evaluations)
       Cyhexatin (Pesticide residues in food: 1981 evaluations)
       Cyhexatin (Pesticide residues in food: 1989 evaluations Part II Toxicology)
       Cyhexatin (Pesticide residues in food: 1991 evaluations Part II Toxicology)
       Cyhexatin (JMPR Evaluations 2005 Part II Toxicological)