WORLD HEALTH ORGANIZATION WHO Food Additives Series 1972, No. 1 TOXICOLOGICAL EVALUATION OF SOME ENZYMES, MODIFIED STARCHES AND CERTAIN OTHER SUBSTANCES The evaluations contained in this publication were prepared by the Joint FAO/WHO Expert Committee on Food Additives which met in Rome, 16-24 June 19711 World Health Organization Geneva 1972 1 Fifteenth Report of the Joint FAO/WHO Expert Committee on Food Additives, Wld Hlth Org. techn. Rep. Ser., 1972, No. 488; FAO Nutrition Meetings Report Series, 1972, No. 50. The monographs contained in the present volume are also issued by the Food and Agriculture Organization of the United Nations, Rome, as FAO Nutrition Meetings Report Series, No. 50A (c) FAO and WHO 1972 HYDROXYPROPYL DISTARCH PHOSPHATE Modification is carried out with 0.1 per cent. phosphorus oxychloride and 8-10 per cent. of propylene oxide. Cross-linkage would be no greater than is experienced on modification with phosphorus oxychloride alone and ether linkages would probably not exceed 20 per 100 anhydroglucose units. Biological data Biochemical aspects Digestibility was tested in groups of 5 rats fed for 7 days a basal diet supplemented by 0, 1 g and 3 g modified or control starch. No difference in weight gain was observed (Prier, 1961). In a caloric utilization study groups of 10 male rats were fed for 10 days either unmodified starch or starch modified with 3 per cent., 6 per cent. or 8 per cent. propylene oxide as well as 0.0123 per cent. phosphorus oxychloride equivalent to a degree of substitution ranging from 0.085 to 0.23. There was a slight decrease in caloric utilization with increasing degree of modification. Diarrhoea occurred whenever 4 g modified starch was included in the daily ration but also at the 2 g/day level with the more highly modified starches. The relative organ weight of the empty caecum was always increased at the 4 g/day level but also at the 2 g/day level with the 2 highly modified starches. Histological examination of the heart, liver, spleen, kidney and caecum revealed no obvious abnormalities related to treatment. Similar data were obtained in a series of tests using starches modified in the intermediate range of degree of substitution by the use of propylene oxide and phosphorus oxychloride (Porter, 1971). Short-term studies Rat Groups of 10 male rats were fed diets containing 17, 34, 51, or 68 per cent. modified starch for 28 days. Growth and bodyweights were reduced compared with controls at the two highest levels tested. The relative organ weights of empty caeca were raised at all levels tested and the extent of the increase was dose-related. No histological abnormalities were seen in heart, liver, spleen, kidney and caecum (Porter, 1971). Long-term studies None available. Comments Only data from caloric utilization studies are available. Although it might be feasible to use the data from related modified starches to arrive at an evaluation there is a need for at least 1 short-term study at different levels to ensure that no toxicity exists unique to this modified starch. EVALUATION Not possible on the evidence available. Further work required by 1973 Appropriate 90-day studies in 2 species. REFERENCES Porter, M. W. (1971) Unpublished report submitted to WHO by A. E. Staley Manufacturing Co. Prier, R. T. (1961) Unpublished report of Wisconsin Alumni Research Foundation No. 1031347/8, submitted by Stein, Hall & Co., Inc.
See Also: Toxicological Abbreviations Hydroxypropyl distarch phosphate (FAO Nutrition Meetings Report Series 46a) Hydroxypropyl distarch phosphate (WHO Food Additives Series 5) Hydroxypropyl distarch phosphate (WHO Food Additives Series 6) HYDROXYPROPYL DISTARCH PHOSPHATE (JECFA Evaluation)