FAO/PL:1967/M/11/1 WHO/Food Add./68.30 1967 EVALUATIONS OF SOME PESTICIDE RESIDUES IN FOOD THE MONOGRAPHS The content of this document is the result of the deliberations of the Joint Meeting of the FAO Working Party of Experts and the WHO Expert Committee on Pesticide Residues, which met in Rome, 4 - 11 December, 1967. (FAO/WHO, 1968) FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS WORLD HEALTH ORGANIZATION Rome, 1968 NABAM This pesticide wan evaluated toxicologically by the 1965 Joint Meeting of the FAO Committee on Pesticides in Agriculture and the WHO Expert Committee on Pesticide Residues (FAO/WHO, 1965). Additional toxicological information, together with information for evaluation for tolerances, is summarized and discussed in the following monograph addendum. IDENTITY Other relevant chemical properties The standard formulation contains 19 per cent of active ingredient (27 per cent as the hexahydrate) and is formulated to be used as a liquid spray after addition of an equimolar amount of zinc sulphate. Occasionally a zinc-iron sulphate mixture is added. The water soluble salt under aeration conditions has been shown to yield a number of products including ethylenethiuram monosulfide and its polymer, ethylene thiourea disulfide and ethylene thiourea (Thorn and Ludwig, 1962). Addition of zinc sulphate to the aqueous solution of the tank mix of the soluble sodium salt yields the relatively insoluble zinc ethylene bisdithiocarbamate. Therefore except for possible by-products formed initially from the sodium salt, the properties of the final spray are essentially those of zineb and for further discussion reference should be made to the monograph on zineb. EVALUATION FOR ACCEPTABLE DAILY INTAKES Biochemical aspects Nabam, in vitro, completely inhibited dopamine beta hydroxylase, prepared from beef adrenals, at a concentration of 4 × 10-6M. Partial inhibition occurs at a concentration of 1 × 10-6M. In rat brain, in vivo, this inhibitory effect is produced only by relatively high doses (300 mg/kg body-weight) (Truhaut et al, 1967). Comment No long-term studies on nabam have been reported. There is no basis for estimating an acceptable daily intake for this compound. Where nabam in used in combination with zinc sulfate prior to spraying, it is converted to zineb and hence residues of nabam would not be present as a result of this use. For this method of use, there is no need for an ADI for nabam, per se, and the ADI estimated for zineb would be applicable. TOXICOLOGICAL EVALUATION Temporary acceptable daily intake for man 0 - 0.025 mg/kg body-weight (as zineb alone or in combination with other ethylene bisdithiocarbamates) This value is based on experiments carried out with zineb and does not take account of chemical alterations after application. Further work required Studies of the compound in plants to determine the chemical nature of the residues followed by appropriate toxicological studies. Results of the above work should be made available not later than 30 June 1971 after which a re-evaluation of this compound will be made. The re-evaluation may be made at an earlier meeting should relevant information become available. EVALUATION FOR TOLERANCES USE PATTERN Pre-harvest treatments The nabam-zinc sulphate tank mix is used as a pre-harvest spray to protect a number of agricultural crops from invasion by fungal plant pathogens. Residues are reduced as outlined under zineb. METHODS OF RESIDUE ANALYSIS The method is identical with that for zineb involving the colorimetric measurement of the carbon disulfide liberated on acid treatment of the residue. (Gordon, Schuckert and Bornak, 1967). NATIONAL TOLERANCES Nabam is registered for use in Canada on the same crops as for zineb except wheat, collards, mustard greens, lettuce and spinach, with a tolerance limit of 7 ppm (Canada, 1967). In the United States, except for lettuce, mustard greens, Swiss chard and spinach with a tolerance limit of 25 ppm, the numerous other vegetables and fruit have a limit of 7 ppm (USDA, 1966) RECOMMENDATIONS FOR TOLERANCES No tolerances for nabam per se are necessary, since this compound is converted to zineb. See monograph on latter compound. REFERENCE PERTINENT TO EVALUATION FOR ACCEPTABLE DAILY INTAKES Truhaut, R., Baquet, A., Bohnon, C. and Guirinot F. (1967) Paper given at IUPAC Congress, Prague, September, 1967 (in press) REFERENCES PERTINENT TO EVALUATION FOR TOLERANCES Canada. (1967) Food and Drug Directorate, T.I.L. 290, Sept. 15. FAO/WHO. (1965) Evaluation of the toxicity of pesticide residues in food. FAO Meeting Rept. PL/1965/10/1; WHO/Food Add./27.65. Gordon, C.F., Schuckert, R.J., and Bornak, W.E. (1967) Improved method for the determination of ethylenebisdithiocarbamate residues in plants, fruits and vegetables. J. Assoc. Off. Anal. Chem. 50: 1102-1108. Thorn, G.D. and Ludwig, R.A. (1962) The dithiocarbamates and related compounds. Amsterdam, Elsevier Pub. Co. 234 p. USDA. (1966) Summary of Registered Agricultural Pesticide Chemical Uses, 2nd ed. Suppl. 2 and 3.
See Also: Toxicological Abbreviations Nabam (FAO Meeting Report PL/1965/10/1)