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    PROPINEB            JMPR 1977

    IDENTITY

    Chemical name

    zinc propylenebisdithiocarbamate

    Synonyms

    (R) Antracol
    Methylzineb
    Mezineb
    LH 30/Z
    Bay 46 131

    Structural formula


           S              S
           |              |
    (-Zn-S-C-NH-CH2-CH-NH-C-S-)X
                    |
                   CH3

    Empirical formula

    (C5H8N2Zn)X

    Other information on identity and properties

    Composition         Satisfactory information was available to the
                        Meeting

    Appearance          White to yellowish powdery practically odourless

    Molecular Weight    289.8 (for the theoretical monomeric compound)

    Melting point       The technical product undergoes degradation,
                        accompanied by brown discoloration, above about
                        160°C; at about 300°C, the organic components of
                        the product sublime, leaving a very small
                        inorganic remainder of ZnS.

    Solubility          Practically insoluble in all usual solvents

    Stability           Unstable in strongly alkaline and strongly acid
                        conditions

    EVALUATION FOR ACCEPTABLE DAILY INTAKE

    Propineb, a propylene analogue of zineb, has moderate acute toxicity.
    It degrades to propylene thicurea (PTU).

    Orally administered propineb is readily excreted via urine and faeces.
    Propineb has a goitrogenic effect. Following oral administration, high
    tissue concentration in, and enlargement of the thyroid and pituitary
    glande occurred. These effects are reversible.

    A dominant lethal test in mice was negative. Reproduction studies in
    rats show no adverse effect at 20 and 60 ppm, but muscular damage
    (myasthenia) and reduced fertility occurred at 200 ppm. In a
    teratogenicity study, a teratogenic effect was produced only at a dose
    which was highly toxic and lethal to the dams. In a long-term study, a
    significent increase in the incidence of thyroid benign tumours and
    skeletal muscle degeneration was observed at dietary level of 1000 ppm
    and above. Significant thyroid enlargement occurred in the males at
    dietary levels of 100 ppm and above. In another two-year feeding
    experiment with rats at a level of 100 ppm, serum protein-bound iodine
    (PBI) was lowered.

    Two studies in dog were carried out (four months and two years). No
    adverse effects were seen at 3000 ppm. In a study with rats for two
    years, a dose-dependent reduction of growth and significant increase
    in liver and kidney weights were noted at 100 ppm and above.

    In a four-week study with hens, no effects were seen at 1000 ppm and a
    dose-dependent increase in thyroid weight occurred at 2000-8000 ppm.
    No neurotoxicity was observed.

    Because of the concern of the Meeting regarding the potential for
    thyrotoxicity and tumorigenicity of PTU (known to occur with ETU), the
    Meeting recommended a temporary ADI for humans.

    TOXICOLOGICAL EVALUATION

    Level causing no toxicological effect

    Rat: 10 mg/kg in the diet, equivalent to 0.5 mg/kg bw
    Dog: 3000 mg/kg In the diet, equivalent to 75 mg/kg bw

    ESTIMATE OF TEMPORARY ACCEPTABLE DAILY INTAKE FOR HUMANS

    0-0.005 mg/kg bw

    RESIDUES IN FOOD AND THEIR EVALUATION

    USE PATTERN

    Propineb is a propylenebisclithiocarbamate fungicide notable for its
    rapidity of action and its long-lasting activity. It has a broad
    spectrum of activity and is especially used as a protective treatment
    for the control of numerous fungal disease pathogens belonging to the
    classes and groups of Oomyoetes, Ascomycetes, Basidiomycetes and Fungi
    imperfecti. Propineb is marketed in more than 70 countries and hence
    is used worldwide.


        TABLE 1. Application rates of propineb on various crops
                                                                                                   
                                               Application rate
                                                                               Recommended
                                                           Number of           pre-harvest
    Crop                Formulation      (in kg a.i./ha)   applications        interval (days)
                                                                                                   
    Bananas             WP               1.4 - 2.1         6 - 14              7
    Pome fruit
    (apples, pears)     WP               1.4 - 2.8         8 - 12              7 - 10
    Cherries            WP               1.4 - 2.8         2 - 3               7
    Plums, damsons      WP               1.4 - 2.8         2 - 5               7
    Peaches             WP               1.4 - 2.8         2 - 3               7 - 10
    Currants(fresh)     WP               2.1 - 2.8         2 - 3               14
    Gooseberries        WP               2.1 - 2.8         2 - 3               14
    Citrus fruit        WP               2.1 - 4.2         3 - 6               14
    Grapes              WP               1.75 - 2.8        4 - 10              21 - 28
                        DP               1.4               6 - 8               21 - 28
    Beans               WP               1.0 - 2.1         3 - 5               7
    Cucurbits           WP               0.84 -1.4         2 - 4               4 - 7
    Celery              WP               0.84 -1.4         2 - 4               7 - 14
    Tomatoes            WP               0.84 -1.75        4 - 8               4
    Onions              WP               1.4 - 2.1         2 - 3               7 - 10
    Potatoes            WP               1.05 - 2.8        4 - 8               7
    Cereals             WP               1.4 - 2.1         1 - 3               28 - 35
    Rice                WP               1.4 - 2.1         1 - 3               21
    Groundnuts          WP               1.4 - 2.1         4 - 6               14
    Coffee              WP               2.3               4                   4 - 7
    Tobacco             WP               2.1 - 2.8         10 - 16             7
                        DP               3.0 - 4.0         10 - 16             7
    Hops                WP               1.5 - 5.6         10 - 16             7

    Post-harvest treatments
                                         Dose              Number of
    Crop                Formulation      (in g. a.i./ha)   applications
    Currants (fresh)    WP               2.1 -2.4          2
                                                                                                   
    WP= Wettable powder
    DP= Dust

    TABLE 2. Residues of propineb in crops after application of 70% WP formulation (Supervised Trials: Bayer, 1977)

                                                                                                                            

                   Application rate      No. of       Days after                      Residue (mg/kg)
    Crop                kg/ha          applications  application    range                                     mean
                                                                                                                            

    Apples              2.1            12            0              n.d. - 5.4             (3)                3.2
                                                     4              1.5 - 2.7              (3)                2.2
                                                     7              1.7 - 2.4              (3)                2.0
                                                     10             1.1 - 2.2              (3)                1.5
                                                     14             0.9 - 2.3              (3)                1.6

    Bananas             2.35 - 3.53    4             1              n.d.                   (6)                n.d.
    Pulp                                             3              n.d.                   (6)                n.d.
                                                     7              n.d.                   (6)                n.d
                                                     14             n.d.                   (6)                n.d
                                                     21             n.d.                   (6)                n.d.
    Peel                                             1              n.d. - 0.5             (6)                0.2
                                                     3              n.d. - 0.4             (6)                <0.2
                                                     7              n.d. - 0.55            (6)                0.2
                                                     14             n.d. - 1.35            (6)                0.3
                                                     21             n.d. - 0.35            (6)                <0.2

    Total 1)            2.1 - 3.15     12            0 - 3          n.d.- <0.2             (20)               <0.2
                                                     7              n.d.- <0.2             (6)                <0.2
                                                     12             n.d.- <0.2             (3)                <0.2

    Beans               1.05           2 - 3         0              0.9 - 1.0              (2)                1.0
                                                     3              0.2 - 0.8              (2)                0.5
                                                     7              n.d. - 0.3             (3)                0.1
                                                     14             n.d.                   (3)                n.d
                                                     21             n.d.                   (3)                n.d.

    Celeriac            1.05           2 - 4         0              n.d.                   (3)                n.d
    Edible root                                      14             n.d. - 0.2             (6)                <0.1
                                                     21             n.d.                   (6)                n.d
                                                     28             n.d.                   (3)                n.d
                                                     35             n.d.                   (3)                n.d

    TABLE 2. (Continued)
                                                                                                                            

                   Application rate      No. of       Days after                      Residue (mg/kg)
    Crop                kg/ha          applications  application    range                                     mean
                                                                                                                            

    Celeriac            1.05           2 - 4         0              16.5 - 25.7            (3)                21.7
    leaf                                             14             0.2 - 0.4              (3)                0.3
                                                     21             0.2 - 0.3              (3)                0.2
                                                     28             0.1 - 0.3              (3)                0.1
                                                     35             n.d. - 0.2             (3)                <0.1

    Cherries            2.8            3             0              2.2 - 14.0             (5)                7.4
                                                     7              0.6 - 2.3              (5)                1.7
                                                     14             0.2 - 0.5              (5)                0.3
                                                     21             0.1 - 0.3              (5)                0.2
                                                     28             n.d. -<0.1             (3)                <0.1

    Citrus              2.52 -4.2      2 - 4         78/79          0.08- 0.17             (2)                0.13
    (Satsuma Orange)                                 124/228        0.07                   (2)                0.07
    Pulp
    Peel                                             78/79          0.09-0.18              (2)                0.14
                                                     124/128        0.06-0.31              (2)                0.19

    Juice                                            78/79          < 0.05                 (1)                0.05

    Coffee              21 g/shrub     4             6              n.d.                   (1)                n.d.
    (beans, hulled)

    Cucumber            1.4            3             7              n.d.                   (1)                n.d.
                        6X 0.1
                        g/plant        6             3              n.d. - <0.3            (2)                n.d.

    Blackcurrants       2.8            2 - 3         0              11.8 - 38.6            (3)                25.2
                                                     7              4.35 - 17.4            (4)                11.7
                                                     11-14          1.2 - 13.4             (6)                8.7
                                                     21             0.65 -10.3             (5)                6.3
                                                     28             0.25-10.7              (5)                5.0
                                                     38-39          0.4 - 1.1              (2)                0.75

    TABLE 2. (Continued)
                                                                                                                            
                   Application rate      No. of       Days after                      Residue (mg/kg)
    Crop                kg/ha          applications  application    range                                     mean
                                                                                                                            
    Red currants        2.8            2 - 3         0              7.2 - 34.2             (3)                20.8
                                                     7              2.15 - 22.6            (4)                10.2
                                                     11-14          1.9 - 16.4             (10)               6.3
                                                     21             0.4 - 9.9              (7)                3.8
                                                     28             0.3 - 7.6              (7)                3.5
                                                     39             0.3 - 1.2              (2)                0.75

    Grapes              2.1 - 3.5      6 - 10        0              4.3 - 25.45            (9)                15.2
                                                     14/15          2.05 -11.2             (9)                7.6
                                                     28             0.95 -8.2              (9)                5.0
                                                     35             0.6 - 5.3              (7)                3.1
                                                     42-78          n.d. - 6.15            (15)               3.2

    Hops                1.5 - 5.6      9 - 20        0 - 6          76.5 - 475             (3)                253
                                                     7 - 9          86 - 309               (4)                175
                                                     14             14                     (1)                14

    Sweet melon
    Pulp                1.4            3             7              n.d.                   (1)                n.d.
    Peel                                             7              0.2                    (1)                0.2

    Onions              2.52           10 - 14       7              0.03                   (2)                <0.03
                                       7 - 13        12             0.03 - 0.06            (2)                0.05
                                       7 - 13        20             0.03                   (2)                0.03

    Peaches             2.1 - 2.8      3             0              2.45 - 30.5            (3)                16.9
                                                     4 - 7          0.2 - 9.9              (4)                4.9
                                                     14             0.4 - 3.8              (3)                2.4
                                                     21             1.3                    (2)                1.3
                                                     28             0.85 - 5.0             (2)                2.9
    Plums               2.8            3             0              1.0 - 1.4              (3)                1.2
                                                     7              1.0 - 1.8              (3)                1.3
                                                     14             0.65 - 0.7             (3)                0.7
                                                     21             0.35 - 0.55            (3)                0.4
                                                     28             0.25 - 0.4             (3)                0.3

    TABLE 2. (Continued)

                                                                                                                            

                   Application rate      No. of       Days after                      Residue (mg/kg)
    Crop                kg/ha          applications  application    range                                     mean
                                                                                                                            

    Potatoes            1.26           6             7              n.d.                   (3)                n.d.
                                                     22             n.d.                   (1)                n.d.
                                                     51-60          n.d.                   (2)                n.d.

    Tobacco             0.2 - 0.4      7 - 17        2 - 7          90 - ca.4000           (28)               625
                        1.3 - 4.6      11 - 18       3 - 5          64.9 - 465             (3)                224
                                                     12             45.4                   (1)                45.4
                                                     17             158.5                  (1)                158.5

    Tomatoes            1.4            6             0              0.2 - 0.55             (3)                0.5
    Greenhouse                                       3              0.3 - 0.65             (3)                0.5
                                                     4              0.4 - 0.8              (3)                0.6
                                                     7              0.3 - 0.75             (3)                0.45

    Field-grown         1.75           5             0/1            2.2 - 3.35             (2)                2.8
                                                     3              1.65 - 2.3             (2)                2.0
                                                     7              0.4 - 1.8              (2)                1.1

    Wheat               1.575 - 2.1    1 - 4         0              37.5 - 53.0            (3)                43.3
    Ears                                             7              7.15 - 10.35           (3)                8.45

    Grains                                           26             0.3                    (1)                0.3
                                                     38-42          n.d. - 0.15            (6)                0.1
                                                     47-68          n.d. - 0.5             (9)                0.1
    Straw                                            42-68          0.85 - 1.6             (6)                1.1
                                                                                                                            

    1) The peel was analyzed, and the residue found was converted to whole fruit.

    

    Pre-harvest treatments

    Propineb is formulated as a wettable powder and as a dust, with the
    wettable powder formulation being used predominantly. The major uses
    of propineb are in viticulture, fruit-growing and vegetable-growing.
    It is used also on a wide range of other crops including tobacco,
    hops, rice, groundnuts and coffee. The application rates for the
    different crops are listed in Table 1. The large variation in the
    numbers of applications per season is accounted for not only by the
    many different diseases on each crop but also by the very large
    differences in infection conditions from one region to another and
    also from year to year.

    RESIDUES RESULTING FROM SUPERVISED TRIALS

    Data on residues of propineb (determined as evolved CS but expressed
    as propineb) in various crops following supervised treatments with the
    wettable powder are shown in Table 2. The figures in brackets
    represent the number of analytical results reported (Bayer, 1977).

    Other uses

    Propineb is also recommended for use on ornamentals (WP formulation).

    FATE OF RESIDUES

    In plants

    The distribution and metabolism of (14C) propineb was studied on
    apples and grapes after a single application in 1972/73 (Vogeler et
    al., 1977a), and on grapes after three applications in 1976 (Vogeler
    et al., 1977b), under field conditions.

    Degradation of (14C) propylenethiourea (PTU) on apples was also
    studied (Vogeler et al., 1977a). The studies were model experiments
    under field conditions to investigate metabolism and not designed to
    make quantitative determinations of metabolite residues after
    commercial application of propineb.

    Whereas propineb remains mainly on the fruit surface, some of the
    metabolites formed can penetrate, at least partially, into the pulp.
    Degradation of propineb results in the formation of a metabolite which
    has not yet been identified, and also the following known metabolites:
    propyleneurea (PU), PTU, 4-methylimidazoline, and the methyl compounds
    of DIDT (5,6-dihydro-3H-imidazo (2,1-C)-1,2,4-dithiyole-3-thione) and
    Jaffé's base (3-(2imidazolin-2-yl)-2-imidazolidinethione. In the 1976
    study involving three applications to grapes, the metabolites were
    found to be present in the grapes in the following percentages at
    harvest:

                                                                                                

                                                 Methyl
    Propineb       4-methyl-      Unidentified   compound       PU      PTU    Methyl
                   imidazoline    metabolite     of Jaffé's                    compound
                                                 base                          of DIDT
                                                                                            

    41             1.7            7              4.6            4.3     9      9
                                                                                            

    
    In the studies conducted in 1972-73, the unidentified metabolite
    occurred in a larger amount than in the 1976 study. The methyl
    compounds of DIDT and Jaffé's base were detected only in the 1976
    study. 4-methylimidazoline is formed in the isolation step as a result
    of a transformation of the unidentified metabolite, which is not
    stable as a free base. It is presumed that this compound is also
    formed from this metabolite in the plant.

    Degradation of PTU on apples proceeds rapidly and as it results in the
    formation of the same unidentified metabolitel it is assumed that
    propineb is degraded mainly via PTU. The residue level of PTO is
    governed mainly by the formation of PTU from propineb and the further
    degradation of PTU.

    In soil

    In field studies, propineb was found to undergo relatively fast
    degradation with half-lives in the range 2-8 clays, while in similar
    studies conducted in Japan, a halflife of about 20 days was recorded
    after five applications (Bayer, 1977). The half-lives in laboratory
    degradation studies were 1 and 9 days while under experimental
    conditions in Japan they were less than 1 day (Bayer, 1977).

    In leaching studies conducted with propineb in which 200 mm of water
    (simulating rainfall) vas passed through 300 mm-high soil columns in 2
    days no propineb was found in the leachate (Bayer, 1977).

    In metabolic studies conducted with (14C) propineb in two standard
    soils it was degraded to PU to the extent of more than 50% of the
    amount applied (Vogeler, 1976). In addition, formation of CO2 was
    noted. Small amounts (about 4%) of PTU were found. Other metabolites
    occurred only 2 in amounts equivalent to less than 4% of the applied
    propineb; they were not identified.

    Degradation of (14C) PTU also resulted in the formation mainly of PU
    and CO2. At the end of the study period, PTU was no lonjer
    detectable. Pr)m the comparison of-the results of the degradation
    studies with propineb mid PTU, it is concluded that degradation of
    propineb proceeds mainly via PTU.

    The behaviour of (14C) propineb in field lysimeters with cores of
    undisturbed soil was studied over a period of 22 months by
    Mittelstaedt and Führ (1977). The total radioactivity showed a loss of
    43% in follow soil with no plant cover and a loss of 26% in soil
    covered with grass. This loss may be attributed largely to the
    degradation of propineb CO2, because laboratory studies revealed that
    39% of the applied propineb was degraded to C02 after 74 days. The
    radioactivity still present in the field soil after 22 months was
    retained largely in the upper 5 cm layer. Of this radioactivity, only
    about 10% was extractable. Heavy absorption or incorporation in the
    organic matter of the soil probably prevented further translocation or
    leaching. Of the radioactivity originally applied, 1.8% was recovered
    in the leachate of the soil with no plant cover and 0.3% was recovered
    in that of the covered soil. From the results of an additional
    laboratory study (Vogeler, 1977), it is assumed that the radioactivity
    in the leachates was accounted for by PU and other polar compounds.

    METHODS OF RESIDUE ANALYSIS

    Propineb residues can be determined by the well-known CS2 evoluation 
    methods such as that on Keppel (1969), in which stannous chloride used
    as a reducing agent.

    A modified procedure based on the Keppel method has recently been
    proposed (Thier, 1977).

    Propylene thiourea can be determined by gas chromatography, without
    derivatization, by an adaptation of the method of Otto et al. (1977).
    The sample is extracted with methanol and the methanol evaporated. The
    remaining water phase is shaken out with n-hexane and cleaned up on an
    aluminium oxide column. The aqueous eluate is concentrated and shaken
    out with dichloromethane. After evaporation of the dichloromethane,
    the residue is dissolved in methanol and PTU directly determined by
    gas chromatography.

    NATIONAL TOLERANCES REPORTED TO THE MEETING

    The following national tolerance and pre-harvest intervals were
    reported to the Meeting.

                                                                                               
    Country             Crop                   Tolerance,                    Pre-harvest
                                               mg/kg                         interval
                                                                             days
                                                                                           
    Australia           General                                              3

    Austria             General                                              14
                        Vegetables,
                        fruit crops              2.0  (dithiocarbamates
                                                      calculated totally
                        Other crops              0.05 as carbon
                                                      disulphide)

    Belgium             Cereals                  0.2 calculated as CS2
                        Fruit, vegetables,
                        excl. potatoes and
                        citrus                   2.0 as CS2
                        Potatoes                 0.5 as CS2
                        Field-grown:
                        Grapes, hops                                         42

                        Leek, onions
                        celery, asparagus -                                  28
                        shallot, beans,
                        cereals, spinach

                        Gherkins                                             10
                        All other crops                                      14
                        Field-grown and
                        under glass:
                        Lettuce, endive from      1.3 - 1.11                 28
                        Under glass:
                        Lettuce, endive from      1.11 - 1.3                 42
                        Radish, spinach                                      28
                        Tomatoes, gherkins,
                        cucumbers                                            3

                                                                                           

    Country             Crop                   Tolerance,                    Pre-harvest
                                               mg/kg                         interval
                                                                             days
                                                                                           

    German
    Democratic
    Republic (GDR)      Field crops -
                        Potatoes                                             7
                        Top fruit and
                        vegetables                                           10
                        Small fruit, leafy
                        vegetables                                           21
                        Fruit-producing
                        vegetables                                           4
                        Grapes                                               14
                        Crops for production
                        of baby
                        foods and dietary
                        foods                                                28
                        Crops for production
                        of drugs                                             21
                        Forage crops,
                        grassland (pastures)                                 7
                        Pome fruit, small
                        fruit, strawberries,
                        stone fruit, nut
                        crops, root
                        vegetables,
                        leafy vegetables,        3.0 calculated as
                        brassicas, legumes       carbon disulphide
                        (pulse crops),
                        fruit-producing
                        vegetables
                        Citrus fruit,
                        cereals,
                        milled grain
                        products,
                        potatoes, onions,
                        vegetable and
                        animal fats,             0.1 calculated as
                        meat, fish, eggs,        carbon disulphide,
                        milk, baby foods

                                                                                           

    Country             Crop                   Tolerance,                    Pre-harvest
                                               mg/kg                         interval
                                                                             days
                                                                                           

    Germany Federal
    Republic of (FRG)   Potatoes                                             7
                        Pome fruit                                           10
                        Stone fruit, small
                        fruit                                                21
                        Grapes                                               42
                        Tomatoes
                        (field-grown
                        and under glass)                                     4
                        Celery                                               28
                        Hops, tobacco                                        7

                        Vegetables, excl.
                        cucumbers
                        and tomatoes             2.0 dithiocarbamates,
                        Fruit crops              2.0 thiuram disulphides
                        Cucumbers and
                        tomatoes                 1.0 calculated totally
                        Other food crops         0.05 as carbon disulphide

    Hungary             General                  3.0
                        Tomatoes (processing)                                8
                        Peppers (processing)                                 10
                        Fruit crops and grapes                               30
                        All other crops                                      21

    Israel              General                                              unrestricted

    Italy               General                                              7

    Mexico              Avocado, cocoa, lemons,
                        mandarin oranges,

                        bergamot oranges,                                    7
                        mango, apples, pears,
                        potatoes


    Netherlands         Potatoes, cereals          0.5 as CS2
                        All other crops            2.0 as CS2

    New Zealand         General                                              7

                                                                                           

    Country             Crop                   Tolerance,                    Pre-harvest
                                               mg/kg                         interval
                                                                             days
                                                                                           

    Norway              General                                              14

    Portugal            General                                              7

    South Africa        General                   3.0
                        Wine grapes                                          28
                        Table grapes                                         28-42
                        Tomatoes, potatoes                                   3
                        Boysenberries,
                        youngberries                                         14

    Spain               General                                              10

    Sweden              General                                              30

    Switzerland         Grapes                   3.0                         21
                        Vegetables,
                        excl. lettuce                                        21

    Yugoslavia          Tobacco                                              7*
                        Grapes                                               42*
                        All other crops                                      14*
                                                                                           

    * (official recommendation)

    
    APPRAISAL

    Propineb is a broad specturm propylenebisdithiocarbamate fungicide
    which is marketed world-wide. Major uses are pre-harvest treatments of
    fruits and vegetables and in viticulture. The wide variation in
    application frequency (1 to 16 applications) is due to the different
    natures and extent of the fungi against which it is aimed.

    Residues data were provided from supervised trials of propineb
    applications to a range of crops. Residues observed, expressed as
    propineb, varied from up to 10 mg/kg on grapes and blackcurrants to
    less than 0.05 mg/kg (limit of determination) in banana pulp.

    The distribution and metabolism of propineb residues in apples and
    grapes have been studied, together with the associated
    propylenethiorurea (PTU) in apples. Whereas the parent propineb and
    PTU remain mainly on the surface, some other metabolites (of which
    several are described) can penetrate at least partially into the flesh
    of the fruit. PTO degrades rapidly to yield the same metabolites (some
    as yet unidentified) as does propineb, suggesting that the degradation
    of propineb may proceed via PTU.

    Field studies of propineb degradation in soil have shown half-lives
    ranging from 2 to 20 days depending on conditions. Over 50% was
    converted to propyleneurea (PU) during similar studies using
    0-14 labelled propineb or PTU.

    Residue analysis is carried out by CS evolution procedures based on
    that of Keppel (1969). A gas-chromatographic method for ETU (Otto et
    al., 1977) has been adapted for the determination of PTU residues.

    RECOMMENDATIONS

    As the analytical method depends on CS2 evoluation, temporary maximum
    residue limits identical to those of the other dithiocarbamates are
    recommended (see "Dithiocarbamate fungicides"). The limits are for
    CS2.

    FURTHER WORK OR INFORMATION

    REQUIRED (by July 1980)

    1.   A study of the mechanism of action of propineb on the thyroid.
    2.   Further work to elucidate the mode of action of PTU and its
         long-lerm toxicity.
    3.   Information regarding the fate of residues during food
         processing, including cooking.

    REFERENCES

    Bayer, (1971) Data submitted by Bayer AG (unpublished).

    Keppel G.E. (1969) Modification of the Carbon Disulfide evolution
    Method for Dithiooarbamate Residues. J. Ass. Off. Anal. Chem. 52,
    162-167.

    Mittelstaedt, W. and Führ, F. (1977) Verhalten von Propineb in
    bewachsenem und unbewachsenem Boden. Landwirtroh. Forsoh., in press.

    Otto, S., Keller, W. and Drescher, N. (1977) A new gas chromatographic
    determination of ethylenethiourea residuee. J. Environ, So. Hlth., B
    12 (3): 179-191.

    Thier, H.P. (1977) Veröffentlichungen aus den Arbeitsgruppen sur
    RUckstandsanalytik der Pestizide in Lebensmitteln. 5. Mitteilung:
    Nalytik von DithiocarbamatRückstäden. Lebensmittelchem. gerichtl.
    Chem. 3-1, 25-27.

    Vogeler, K. (1976) Zum Metabolismus von Propineb, Zineby
    Propylenthioharnstoff und Athylenthioharnstoff im Boden nach
    BBA-Plerkblatt Nr. 36. Bayer AG, Pflanzenschutz-Anwendungstechnik,
    unpublished report RA 726, September, 1976.

    Vogeler, K. (1977) Leachine-Laborversuche mit (140) Propineb Bayer AG;
    Pflanzenschutz-Anwendunstechnik, unpublished report RA-573, July 20,
    1977.

    Vogeler K., Drezer Ph., Papp, R.l Steffan, E., Ullerneyer, H.
    Verteilung und Metabolismum von Propineb in Apfeln und Weintrauben
    sowie der Abbauprodukte Propylenthioharnstoff und Athylenthioharnstoff
    in Apfeln. Pflanzenschutz-Nachrichten Bayer 30, 72-97.

    Vogeler K., Rapp, A., Steffan, H., Ullemeyer, H. Der Metabolismus von
    Propineb in Weintrauben nach dreimaliger Applikation von. Antracol im
    Freiland. Bayer AG, Pflanzenschutz-Anwendungstechnik, unpublished
    report RA-363, April 28, 1977b.
    


    See Also:
       Toxicological Abbreviations
       Propineb (Pesticide residues in food: 1984 evaluations)
       Propineb (Pesticide residues in food: 1985 evaluations Part II Toxicology)
       Propineb (Pesticide residues in food: 1993 evaluations Part II Toxicology)