PESTICIDE RESIDUES IN FOOD - 1984 Sponsored jointly by FAO and WHO EVALUATIONS 1984 The monographs Data and recommendations of the joint meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Expert Group on Pesticide Residues Rome, 24 September - 3 October 1984 Food and Agriculture Organization of the United Nations Rome 1985 ISOFENPHOS Explanation The CCPR, at its sixteenth Session, asked the JMPR to consider whether the residue definition, currently "sum of isofenphos and its oxygen analogue" should be extended to include the metabolites des-N-isopropyl-isofenphos (DNI) and its oxygen analogue (DNIOA). It was pointed out that crop rotation metabolism studies had suggested that residues of DNIOA sometimes exceeded those of isofenphos and its oxygen analogue in some crops. RESIDUES IN FOOD AND THEIR EVALUATION Many of the data supporting the 1981 and 1982 evaluations were based on analyses for isofenphos and its oxon only. When DNI and DNIOA were also determined, the levels of all four compounds were often below the limit of determination. Data from supervised trials in which at least one of them was at a measurable level were available for maize and sweet corn grain, husks, cobs and forage, wheat, sorghum, soybeans and onions. Of a total of 93 samples, only one (onion) contained a measurable level of DNI: it was at the limit of determination (0.01 mg/kg) and represented about 1 percent of the residue. DNIOA was measured in 24 samples of corn forage, husks and kernels at levels ranging from 0.01 to 0.1 mg/kg. It represented 10 percent of the residue or less in 4 samples, and 10-25 percent in 14 samples of forage with total residues of 0.06-0.4 mg/kg. It was found in forage at 0.02 and 0.08 mg/kg in total residues of 0.07 and 0.17 mg/kg, and was the only residue measured in the remaining four samples (maize husk, kernels and forage), at levels of 0.01-0.03 mg/kg. In metabolism studies on sweet corn, onions and cabbage, DNIOA was a minor metabolite reaching a maximum of 10 percent of the sum of isofenphos, its oxon and DNIOA in sweet corn, 4 percent in onions and 5 percent in cabbage. DNI was not detected. In feeding studies with hens and a pig, the main intact ester residue was isofenphos. DNI accounted for a maximum of about 5 percent of the total organophosphorus residue in tissues and eggs. DNIOA was not found. In a cow feeding study, the only identified organophosphorus residue was isofenphos oxon in liver and kidney. In metabolism studies with beans, sugar beet and wheat as rotational crops, isofenphos, its oxon, DNI and DNIOA were determined by GLC. Isofenphos oxon was the main component of the residue in most samples. DNIOA accounted for 11 percent, 18 percent and 17 percent of the total residue in wheat heads, stems and leaves respectively, and for 75 percent of a total residue of 0.08 mg/kg in sugar beet tops. It was below the limit of determination in sugar beet roots and bean forage. DNI was below the limit of determination in all samples. It is clear that inclusion of DNI in the residue definition is unnecessary. DNIOA occasionally forms a substantial proportion of the residue, but only apparently when the total residue is low. It occurs mainly in forage, for which the JMPR has not proposed an MRL. (There is a proposed TMRL of 0.5 mg/kg for maize fodder and sweet corn fodder.) RECOMMENDATION The definition of the residue should not be changed.
See Also: Toxicological Abbreviations Isofenphos (Pesticide residues in food: 1981 evaluations) Isofenphos (Pesticide residues in food: 1982 evaluations) Isofenphos (Pesticide residues in food: 1986 evaluations Part II Toxicology)