ACEPHATE EXPLANATION Acephate was evaluated for acceptable daily intake by the Joint Meeting in 1976 when an ADI of 0-0.2 mg/kg bw was allocated (Annex I, FAO/WHO, 1977b). The ADI was derived from no-effect levels based on studies performed by Industrial Bio-Test Laboratories (IBT). Further data were evaluated in 1982, when a temporary ADI of 0-0.003 mg/kg bw was allocated (Annex I, FAO/WHO, 1983a). The temporary ADI was reduced to 0-0.0005 mg/kg bw in 1984 (Annex I, FAO/WHO, 1985c) in view of the inadequacy of the multigeneration study and the lack of a delayed neurotoxicity study. An ADI at the level of 0-0.003 mg/kg bw was allocated in 1987 when multigeneration reproduction, delayed neurotoxicity and lifetime carcinogenicity studies were submitted for evaluation by the Joint Meeting (Annex I, FAO/WHO, 1988b). Since a cholinesterase study in human volunteers by IBT has been validated (Cavalli, 1978), it was reconsidered. Statistical analysis of in vitro cholinesterase inhibition data, which were previously reviewed by the Joint Meeting in 1984 (Annex I, FAO/WHO, 1985c), has also been reconsidered. EVALUATION FOR ACCEPTABLE INTAKE BIOCHEMICAL ASPECTS Effects on enzymes Regression analysis of in vitro data on inhibitory activity of acephate technical on brain and red blood cell (RBC) acetyl- cholinesterase (ACHE) and plasma cholinesterase (ChE) from rat, monkey and human tissues was performed. Calculated I50 are reported in Table 1 (Griffis, 1988). Table 1. I50 values (37°C, 60', pH 8.0) for acephate techmical I50 (mM) (95% confidence limits) Brain RBC AChE Plasma rat 1.25-1.72 1.04-1.47 2.84-5.22 monkey 2.78-3.38 1.90-3.17 1.43-2.49 human 3.88-5.22 2.41-2.87 1.56-1.76 BIOLOGICAL DATA Special study on delayed neurotoxicity In two experiments, groups of 6 adult hens were given by gavage 200 or 700 mg/kg bw acephate technical (98% purity). In each experiment a group of 3 hens received DFP (0.200 mg/kg bw i.m.). The control animals received only water. Twenty-four hours after dosing, brain neuropathy target esterase (NTE) and AChE of survivors were measured. Results are summarized in Table 2 (Wilson, 1988). Table 2. Hen brain NTE and AChE activities 21 hours after treatment Compound Dose % control (no.) NTE AChE acephate 200 mg/kg (5) 74 18 acephate 700 mg/kg (3) 55 12 DFP 0.2 mg/kg (3+3) 43-46 33-41 Toxicological studies Observations in humans Combinations of acephate and methamidophos were orally administered to human volunteers. They received either a combination (methamidophos/acephate) of 1:9 or 1:4 daily (divided in three equal doses). Controls (two males and two females) received corn oil. The 1:9 group (three males and three females) was administered the combination for consecutive periods of 21 days at grading doses of 0.1, 0.2 and 0.3 mg/kg bw/day followed by a 7-day recovery period after which the dose was increased to 0.4 mg/kg bw/day (females only) for 10 days. The 1:4 group (two males and two females) received the combination at doses of 0.1 and 0.2 mg/kg bw/day for 21 consecutive days. No effect was noted on RBC cholinesterase at any dose. In the 1:9 group, plasma cholinesterase activity was affected at the 0.3 mg/kg dose in males and only slightly at the 0.4 mg/kg dose in females. In the 1:4 group, both males and females showed decreased plasma cholinesterase at the 0.2 mg/kg dose level. The NOAEL in this study was determined to be 0.3 mg/kg bw of acephate in combination with 0.03 mg/kg bw of methamidophos or 0.2 mg/kg bw of acephate in combination with 0.04 mg/kg bw of methamidophos (Garofalo, 1973). The validated raw data support the above conclusions regarding cholinesterase activities. The dosing is also supported by analysis of samples of blood and urine of the study subjects. No information was available to support the claim of no clinical effects at any of the administered doses (Cavalli, 1978). COMMENTS Acephate was evaluated for acceptable daily intake by the Joint Meeting in 1976 when an ADI of 0-0.02 mg/kg was allocated. The ADI was obtained on no-effect levels based on studies performed by Industrial Bio-Test Laboratories (IBT), most of which were later considered to be invalid. An ADI at the level of 0-0.003 mg/kg bw was allocated in 1987 when multigeneration reproduction, delayed neurotoxicity and lifetime carcinogenicity studies were submitted for evaluation by the Joint Meeting. In vitro data showed minimal differences in the sensitivity of rat, monkey and human plasma cholinesterase and erythrocyte and brain acetylcholinesterase to inhibition by acephate. A study in humans demonstrated no effect on erythrocyte acetylcholinesterase at any of the dose levels used (up to 0.3 mg/kg bw in combination with 0.03 mg/kg methamidophos or 0.2 mg/kg bw in combination with 0.04 mg/kg bw methamidophos). Acephate caused inhibition of brain neuropathy target esterase in hens at a dose lower than the LD50. The Meeting was therefore aware of the possibility that in extreme conditions of exposure, which are unlikely to occur in food, acephate may have the potential of causing delayed polyneuropathy. The Meeting was also aware that methamidophos is found as a residue in crops treated with acephate. On the basis of the rat NOAEL (brain acetylcholinesterase), the rabbit NOAEL for teratogenicity, and the human volunteer study, the Meeting agreed to establish an ADI at a higher level. TOXICOLOGICAL EVALUATION LEVEL CAUSING NO TOXOLOGICAL EFFECT Rat: 5 ppm in the diet, equivalent to 0.25 mg/kg bw/day Dog: 30 ppm in the diet, equivalent to 0.75 mg/kg bw/day Rabbit: 3 mg/kg bw/day Man: 0.3 mg/kg bw/day ESTIMATE OF ACCEPTABLE DAILY INTAKE FOR MAN 0-0.03 mg/kg bw STUDIES WHICH WILL PROVIDE INFORMATION VALUABLE IN THE CONTINUED EVALUATION OF THE COMPOUND Further observations in man. REFERENCES Cavalli, R.D. 1978. Data validation Industrial Bio-Test report no. 636-02498. A study on the effects of orthene and monitor on plasma and erythrocyte cholinesterase activity in human subjects during subacute oral administration. Submitted to WHO by Chevron Chemical Company, Richmond, CA, USA (Unpublished). Garofalo, M. 1973. A study on the effects of orthene and monitor on plasma and erythrocyte cholinesterase activity in human subjects during subacute oral administration. Industrial Bio-Test Laboratories, Inc. report no. 636-02498. Submitted to WHO by Chevron Chemical Company, Richmond, Ca, USA (Unpublished) (Validated by EPA, USA). Griffis, L.C. 1988. Statistical analysis of acephate in vitro cholinesterase inhibition data. Chevron Environmental Health Center, Inc. Submitted to WHO by Chevron Chemical Company, Richmond, CA, USA (Unpublished). Wilson, B.W. 1988. Cholinesterase and neurotoxic esterase studies in chickens with Chevron Acephate technical. Preliminary Report submitted to WHO by Chevron Chemical Company, Richmond, CA, USA (Unpublished).
See Also: Toxicological Abbreviations Acephate (ICSC) Acephate (Pesticide residues in food: 1976 evaluations) Acephate (Pesticide residues in food: 1979 evaluations) Acephate (Pesticide residues in food: 1981 evaluations) Acephate (Pesticide residues in food: 1982 evaluations) Acephate (Pesticide residues in food: 1984 evaluations) Acephate (Pesticide residues in food: 1984 evaluations) Acephate (Pesticide residues in food: 1987 evaluations Part II Toxicology) Acephate (Pesticide residues in food: 1990 evaluations Toxicology) Acephate (JMPR Evaluations 2002 Part II Toxicological) Acephate (JMPR Evaluations 2005 Part II Toxicological)