PESTICIDE RESIDUES IN FOOD - 1984 Sponsored jointly by FAO and WHO EVALUATIONS 1984 The monographs Data and recommendations of the joint meeting of the FAO Panel of Experts on Pesticide Residues in Food and the Environment and the WHO Expert Group on Pesticide Residues Rome, 24 September - 3 October 1984 Food and Agriculture Organization of the United Nations Rome 1985 CYHALOTHRIN RESIDUES IN FOOD AND THEIR EVALUATION USE PATTERN Cyhalothrin is a pyrethroid insecticide with a high level of activity against a wide range of Lepidoptera, Hemiptera, Diptera and Coleoptera spp. It also has miticidal activity. The compound is a stomach, contact and residual insecticide. It shows adulticidal, ovicidal and, particularly, larvicidal activity. It is also extremely effective against a great number of insects resistant to standard treatments such as organochlorines and organophosphates. Like other photostable synthetic pyrethroids, cyhalothrin is relatively stable to degradation in sunlight. This allows it to be used as a practical tool in agriculture. Cyhalothrin is not plant systemic and has very little fumigant or translaminar activity. The miticidal activity of cyhalothrin is seen as being particularly useful as an adjunct to the control of other insects, which the compound provides in tree fruits such as apples. When used for lepidopterous pest control, it greatly reduces the risk of mite build-up that is experienced with some other insecticides in top fruit and can reduce, or even eliminate, the need for mite sprays. Owing in part to its short persistence in soil and in part to a lack of systemic effect, cyhalothrin is of only limited value when used as a soil insecticide. It can, however, give useful control of cutworms when applied as a crop/ground spray. Cyhalothrin has no molluscicidal or nematicidal activity. Preventive treatments are generally more effective than curative treatments against major pests, such as boring caterpillars or leaf miners. A programme of sprays is usually required, particularly during more active growth stages of the plant and when the potential for re-infestation remains high. Best results are obtained using good spray cover, particularly when cyhalothrin is used during the early development stages of the pest. RESIDUES RESULTING FROM SUPERVISED TRIALS Cyhalothrin residues have been determined in a range of crops taken from supervised trials in several different countries. Samples from trials conducted outside the United Kingdom were frozen and transported by air freight to laboratories in the United Kingdom, where they were stored in a deep freeze (-20°C) until required for analysis. Fresh samples from trials in the United Kingdom were frozen upon receipt. Cyhalothrin residues in crops stored deep frozen have been shown to be stable for a 12-month period (Swaine & Sapiets, 1984). Cyhalothrin residues were determined by gas chromatography using an electron-capture detector, as described under "Methods of Residue Analysis". Pome Fruits At rates of 20-40 mg/kg a.i. in the spray, or 40-80 g a.i./ha, cyhalothrin gives excellent control of a complex of insects on apples, including winter moth (Operophtera brumata), tortrix moths (Archips podana and Adoxophyes orana), codling moth (Cydia pomonella) , apple sucker (Psylla mali) and apple sawfly (Hoplocampa testudinea) . A programme of at least three sprays per season is normally required, with an interval of at least two weeks between the last application and first harvest. As an acaricide, 50-100 mg/kg a.i. in the spray, or at least 100 g a.i./ha, is needed for economic control of fruit tree red spider mite (Panonychus ulmi) and of Tetranychidae. During 1982-83, supervised residue trials on apples or pears were conducted in Cyprus, Japan, South Africa, Spain and the United Kingdom, using either 5 percent W.P. or 10 percent E.C. formulations. At rates needed for lepidopterous insect control, the maximum residue level in apples immediately after application did not exceed 0.5 mg/kg. As in the cases of permethrin and cypermethrin (FAO/WHO, 1980) 1/, cyhalothrin levels on apples decline relatively slowly. Nevertheless, at rates needed for lepidopterous insect control, cyhalothrin levels were consistently below 0.2 mg/kg two weeks after the last application (Table 1) (Swaine et al., 1983a,b,c,d). Results of a trial on pears in Cyprus were also consistent with these findings (Swaine et al., 1983e). In a comparative trial in South Africa, cyhalothrin residue levels on apples and pears were similar after various intervals in the 32 days following the application of a 10 percent E.C. formulation at 40 or 80 mg/kg a.i. in the spray, applied to "run-off" (Table 2) (Swaine et al., 1983b; 1984). Cabbage and Chinese Cabbage At rates of approximately 10-20 g a.i./ha, cyhalothrin gives good control of a range of insects on cabbage, including Spodoptera spp., cabbage loopers (Trichoplusia ni), cabbage moths and butterflies (Plutella and Pieris spp.), aphids (Myzus and Brevicoryne brassicae) and whitefly (Trialeurodes vaporarium). A programme of sprays may be required, depending on the location, pest spectrum and infestation pressure. An interval of two to four days between last spraying and harvest is often possible; in some situations it may be possible to observe an interval as long as seven days. 1/ See Annex 2 for FAO and WHO documentation During 1982-83, trials were conducted in Spain, the United Kingdom and the United States, using either a 5 percent W.P. or 10 percent E.C. formulation. Cyhalothrin was applied at two different rates at each site and there was good correlation within each trial between the initial residue found after the last application and the rate of application. There was greater variation in initial residues between sites. The data indicated over all that cyhalothrin has a relatively shorter initial half-life, of two to five days, on cabbages than on apples. This is consistent with the earlier findings with permethrin and cypermethrin (FAO/WHO 1980). Levels on cabbage were generally below 0.2 mg/kg within a few days of spraying (Table 3) (Sapiets, 1984a,b; Swaine et al., 1983f). There was no obvious build-up in cyhalothrin residue levels on cabbage after repeated applications (Sapiets, 1984b). Other Crops Cyhalothrin residues data are also available on Swiss chard, peaches, grapes, maize, sorghum and tea (Sapiets, 1984c). FATE OF RESIDUES In Plants 14C-cyclopropyl-labelled cyhalothrin, containing approximately 10 percent of the 14C-cyclopropyl-labelled 1RS-cis-E form of the molecule, was formulated as a 10 percent emulsifiable concentrate formulation, diluted with water and spotted onto young growing apple fruits. A polythene shelter erected over the tree protected the fruits from direct rainfall but allowed transmission of the ultraviolet radiation present in sunlight. The surfaces of the apples chosen for treatment were those exposed directly to the predominant direction of incident sunlight. After 56 days, at least 89 percent of the radioactivity recovered from treated peel was due to unchanged pyrethroid. The remainder of the radioactivity extracted with aqueous acetone was polar; acid hydrolysis produced an organosoluble fraction, which contained almost entirely 1RS-cis-3-(Z-2-chloro-3,3,3- trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylic acid, although the presence of about 14 percent of this radioactivity in the 1RS-trans form was evidence of a certain amount of photoisomerization. The applied radioactivity was not translocated away from the treated area of peel. The flesh from below the treated area of fruit harvested at 28 and 56 days after treatment contained less than 1 percent of the recovered radioactivity (Hall & Leahey, 1979). 14C-cyclopropyl-labelled cyhalothrin, prepared as an aqueous dilution of a 10 percent E.C. formulation, was spotted at a rate equivalent to approximately 25 g a.i./ha onto the leaves of cabbage plants grown in pots. The plants were exposed to sunlight but were protected from rainfall and covered overnight with a polythene cover. TABLE 1. Cyhalothrin Residues in Apples from Supervised Trials Country/ Formulation Rate Volume No. Interval last Residues (mg/kg) year (g a.i./ha) of spray spray to (l/ha) sampling (days) R157836 Rl19321 Total Japan 1 5% W.P. 25 mg/kg a.i. 6 300 3 7 0.09, 0.10 0.06, 0.07 0.15, 0.17 1982 in spray 14 0.05, 0.05 0.03, 0.04 0.08, 0.09 (0.0025%) 21 0.02,<0.006 0.01,<0.004 0.03,<0.01 3 000 3 7 0.09, 0.09 0.07, 0.07 0.16, 0.16 14 0.08, 0.08 0.06, 0.06 0.14, 0.14 21 0.09, 0.06 0.06, 0.04 0.15, 0.10 Spain 10% E.C. 25 mg/kg a.i. Sprayed 4 0 0.06 0.04 0.10 1982 in spray to "run- 1 0.05 0.04 0.09 (0.0025%) off" 3 0.04 0.03 0.07 7 0.03 0.02 0.05 50 mg/kg a.i. Sprayed 4 0 0.07 0.06 0.13 in spray to "run- 1 0.07 0.05 0.12 (0.005%) off" 3 0.07 0.06 0.13 7 0.04 0.03 0.07 South 10% E.C. 40 mg/kg a.i. Sprayed 1 0 0.06 0.05 0.11 Africa in spray to "run- 1 0.08 0.06 0.14 1982-83 (0.007%) off" 2 0.06 0.05 0.11 (80 g/ha) 4 0.07 0.05 0.12 8 0.07 0.06 0.13 16 0.06 0.05 0.11 32 0.02 0.02 0.04 TABLE 1. (continued) Country/ Formulation Rate Volume No. Interval last Residues (mg/kg) year (g a.i./ha) of spray spray to (l/ha) sampling (days) R157836 Rl19321 Total 80 mg/kg a.i. Sprayed 1 0 0.21 0.16 0.37 (.0.008%) to "run- 1 0.24 0.19 0.43 (160 g/ha) off" 2 0.24 0.18 0.42 4 0.18 0.13 0.31 8 0.14 0.10 0.24 16 0.11 0.08 0.19 32 0.12 0.09 0.21 United 10% E.C. 45 g a.i./ha 560 1 0 0.03 0.02 0.05 Kingdom 2 7 0.02 0.01 0.03 1982 14 <0.006 <0.004 <0.01 45 g a.i./ha 560 1 0 0.04 0.03 0.07 7 0.02 0.02 0.04 14 0.01 <0.004 <0.014 1x18 g a.i./ha 560 3 14 0.04 0.02 0.06 and 2x45 g a.i./ha 1x24 g a.i./ha 560 2 40 0.01 0.01 0.02 and 1x60 g a.i./ha 25 mg/kg a.i. Sprayed 2 0 0.05, 0.12 0.04, 0.09 0.09, 0.21 in spray to "run- 1 0.06, 0.11 0.05, 0.08 0.11, 0.19 (50 g/ha) off" 3-4 0.04, 0.12 0.03, 0.09 0.07, 0.21 7 0.04, 0.08 0.04, 0.06 0.08, 0.14 TABLE 1. (continued) Country/ Formulation Rate Volume No. Interval last Residues (mg/kg) year (g a.i./ha) of spray spray to (l/ha) sampling (days) R157836 Rl19321 Total 50 mg/kg a.i. Sprayed 2 0 0.12, 0.11 0.10, 0.09 0.22, 0.20 in spray to "run- 1 0.12, 0.16 0.09, 0.13 0.21, 0.28 (100 g/ha) off" 3-4 0.08, 0.17 0.06, 0.12 0.14, 0.29 7 0.07, 0 12 0.06, 0.09 0.13. 0.21 1 The duplicate results are on replicate samples from the same trial 2 The duplicate results are on single samples from two different trials in different locationsœ TABLE 2. Cyhalothrin Residues in Apples and Pears from Supervised Trials, South Africa1 Application Interval spray Total residues Rate Volume No. to sampling (mg/kg) (mg/kg a.i.) of spray (days) Apples Pears 40 Sprayed to 1 0 0.11 0.12 "run-off" 1 0.14 0 17 (80 g/ha) 2 0.11 0.16 4 0.12 0.16 8 0.13 0.16 16 0.11 0.14 32 0.04 0.07 80 Sprayed to 1 0 0.37 0.29 "run-off" 1 0.43 0.29 (160 g/ha) 2 0.42 0.32 4 0.31 0.25 8 0.24 0.21 16 0.19 0.17 32 0.21 0.14 1 All trials done in 1982 using 10 percent E.C. formulation TABLE 3. Cyhalothrin Residues in Cabbage from Supervised Trials Residues (mg/kg) Country/ Rate Volume No. Interval last year Formulation (g a.i./ha) of spray spray to R157836 Rl19321 Total used (1/ha) sampling (days) Cyhalothrin Spain 10% E.C. 20 300 4 0 0.44 0.31 0.75 1983 3 0.25 0.18 0.43 (on 7 0.18 0.13 0.32 Chinese cabbage) 40 300 4 0 0.58 0.43 1.0 3 0.28 0.21 0.49 7 0.28 0.20 0.48 United 1 5% W.P. 75 3 000 2 14 - - 0.06, 0.08 Kingdom 3 0 - - 0.36, 0.34 1983 3 0.01, 0.04 0.01, 0.03 0.02, 0.07 7 0.01, 0.01 0.01, 0.004 0.02, 0.01 14 <0.006,<0.006 <0.004,<0.004 <0.01,<0.01 United 10% E.C. 10 225 6 0 0.07 0.04 0.11 States 1 0.06 0.03 0.09 1982 3 0.02 0.01 0.03 7 0.01 0.01 0.02 20 225 6 0 0.20 0.13 0.33 1 0.05 0.04 0.09 3 0.05 0.02 0.07 7 0.04 0.03 0.07 1 The duplicate results are on single samples from two different varieties on the same site sprayed at the same time. After six weeks, 54 percent of the radioactivity was still present as the intact pyrethroid. Some photoisomerization was indicated by the interconversion of cis and trans isomers and of Z and E isomers, although even after six weeks the combination of the two enantiomer pairs making up cyhalothrin itself still predominated. Cyhalothrin was degraded on cabbage leaves to polar compounds that could be converted to organosoluble materials by acid hydrolysis. 1RS-cis- and 1RS-trans-3-(ZE-2-chloro-3,3,3-trifluoroprop- 1-enyl)-2,2-dimethylcyclopropanecarboxylic acids were the major hydrolysis products, accounting for approximately 8 percent of the radioactivity present (Curl & Leahey, 1983). A cabbage plant was sprayed eight times at weekly intervals with 14C-cyclopropyl-labelled cyhalothrin, prepared as an aqueous dilution of a 10 percent E.C. formulation, at a rate of 25 g a.i./ha. The plant was left exposed to all weather conditions without shelter. One week after the final spray, total radioactive residues in the outer leaves and in the heart were 1.13 and 0.003 mg cyhalothrin equivalents/kg, respectively, equivalent to 0.44 mg/kg on a whole cabbage basis; 80 percent of the radioactive residue in the outer leaves was unchanged cyhalothrin and the pattern of metabolism was the same as for a single application. The effects of photoisomerization were less marked in this study than in the one involving the single application, probably because most of the residues resulted from the final spray (Curl & Leahey, 1983). Evidence for the fate of the alcohol part of the molecule after ester hydrolysis was provided by studies on the structurally-similar compound cypermethrin, which were reviewed by the 1979 meeting and in which 14C-benzyl-labelled material was used. In outdoor studies on lettuce and apples, cypermethrin itself was still the dominant part of the residue after three weeks. On apple fruit and lettuce, most of the remainder of the radioactivity was present as polar materials, which individually were in too small amounts for detailed study. On apple leaves, compounds identified included 3-phenoxybenzoic acid, 3-(4-hydroxyphenoxy)benzoic acid, 3-phenoxybenzaldehyde and 3-phenoxybenzyl alcohol, either free or as conjugates. Samples of apples and peaches from trials in Japan have been analysed for residues of cyhalothrin, 3-phenoxybenzoic acid, 3-(4-hydroxyphenoxy)-benzoic acid and 1RS-cis-3-(Z-2-chloro- 3,3,3-trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylic acid (HCVA). In all cases, residues of the metabolites were very much smaller than those of cyhalothrin (Table 4) (Sapiets, 1984d; Swaine, Sapiets et al., 1983a, h). TABLE 4. Cyhalothrin and Metabolite Residue in Apples and Peaches, Japan 1 Residue (mg/kg) Crop Interval last application to Cyhalothrin HCVA 3-phenoxy- 3-(4-hydroxyphenoxy)- sampling benzoic acid benzoic acid (days) Apple 7 0.16 0.02 <0.02 <0.05 14 0.14 <0.01 <0.02 <0.05 21 0.13 0.01 <0.02 <0.05 7 0.16 0.04 <0.02 <0.02 14 0.09 0.02 <0.02 <0.02 21 <0.02 <0.01 <0.01 <0.02 Peach 7 0.30 0.05 <0.02 <0.02 14 0.25 0.08 <0.02 <0.05 21 0.12 0.03 <0.02 <0.02 7 0.30 0.07 0.02 <0.02 14 0.22 0.03 <0.02 <0.02 21 0.12 0.02 <0.02 <0.02 1 All trials done in 1982 using a 5 percent W.P. formulation In Soil At residue levels that are likely to occur under normal field conditions, cyhalothrin is degraded rapidly in soil. When a sandy loam soil was treated with 14C-cyclopropyl-labelled cyhalothrin, only 28 percent of the recovered radioactivity was present as cyhalothrin after five weeks incubation under aerobic conditions; 30 percent was evolved as 14C-labelled carbon dioxide and 3.5 percent of the recovered radioactivity was due to 1RS-cis-3-(Z-2-chloro-3,3,3- trifluoroprop-1-enyl)-2,2-dimethylcyclopropane-carboxylic acid. Approximately 19 percent of the radioactivity was not extracted using acetonitrile at room temperature followed by soxhlet extraction with aqueous acetonitrile (Bewick & Zinner, 1981). Using soil thick-layer descending chromatography in three soils, cyhalothrin was shown to have low mobility in soil which was considerably less than that of atrazine (Stevens & Poole, 1981). In Water 14C-cyclopropyl-labelled cyhalothrin was incubated with river water/sediment mixtures contained in quartz flasks. The flasks were either exposed to sunlight or were maintained under dark conditions by covering them with aluminium foil. In the dark, degradation of cyhalothrin was slow (over 80 percent unchanged after 32 days). However, when exposed to sunlight the cyhalothrin degraded with a half-life of approximately 20 days in both river water/sediment mixtures. The rate at which the parent compound was lost from the aqueous phase was, however, much more rapid than its rate of degradation in the whole water/sediment system. This was due to the ready absorption of cyhalothrin onto the sediment. The major degradation process was simple ester cleavage of the molecule, producing 1RS-cis- and 1RS-trans-3-(ZE-2-chloro-3,3,3- trifluoroprop-1-enyl)-2,2-dimethylcyclopropanecarboxylic acids. After 32 days irradiation, the compounds together represented 36 to 47 percent of the radioactivity applied to the water/sediment systems. Some photoisomerization also occurred (Hall & Leahey, 1983). In Processing Cyhalothrin residues on cabbage are present mostly in the outer leaves. Therefore, the extent to which these outer leaves are removed before consumption has a major bearing on residues on cabbage at the point of consumption. In a field residue trial in the United Kingdom, cyhalothrin residues were found to be mainly in the seven outermost leaves (Sapiets, 1984b). In the cabbage metabolism work reviewed above, one week after the last of eight weekly sprays of 25 g a.i./ha, total radioactive residues in the outer leaves and in the hearts were 1.13 and 0.003 mg cyhalothrin equivalents/kg, respectively, equivalent to 0.44 mg/kg on a whole cabbage basis. Eighty percent of the radioactivity in the outer leaves was due to unchanged cyhalothrin (Curl & Leahey, 1983). Cyhalothrin levels on cabbage treated in Japan and from which leaves had been stripped before the crop was submitted for analysis were also extremely small (<0.01 mg/kg) (Swaine et al., 1983g). A comparison of cyhalothrin residues from two concurrent trials with the same 10 percent E.C. formulation, applied at the same rates in the same location in Spain on Chinese cabbage, from which outer leaves were not stripped, and on common cabbage, from which leaves were stripped before the crop was submitted for analysis, showed very much larger residues in the former (Table 5) (Sapiets, 1984a,e). METHODS OF RESIDUE ANALYSIS The crops were prepared for analysis by mincing or chopping until a homogeneous sample was obtained. For small fruits the whole sample was prepared; large vegetables, e.g. cabbage, were quartered and opposite quarters taken. Samples were extracted using 50 percent acetone in hexane and the extracts washed with water. Co-extractives were removed by liquid-liquid partition chromatography where necessary (crops requiring this clean-up include cabbage). All crops were cleaned up using Florisil adsorption chromatography. Final quantitative determination of cyhalothrin residues was by gas-liquid chromatography, using electron-capture detection. The limit of determination for total cyhalothrin isomers is 0.01 mg/kg. An internal standard procedure was used for the quantitative measurement of residues. The internal standard compound, a synthetic pyrethroid analogue in which a bromine atom is substituted for the chlorine atom on the vinyl side chain, was added to each sample prior to extraction. The response ratio of cyhalothrin to internal standard in the final extract of each sample was used for quantitative measurement of residues. The use of the internal standard allows the size of the sample aliquot processed through the method to be reduced without loss of accuracy. In addition, as the recovery of pyrethroid is effectively monitored for each sample, the precision of the assay is significantly improved using this procedure. Calibration curves plotted for amount of cyhalothrin versus response ratio of cyhalothrin to internal standard gave linear regression lines (correlation coefficient r > 0.99) with an intercept passing through the origin (Sapiets & Swaine, 1983). APPRAISAL Cyhalothrin is a new photostable synthetic pyrethroid insecticide and acarcide with a level of activity, demonstrated by required dose rates, between deltamethrin and the other related compounds permethrin and cypermethrin. Since it is not systemic, residue levels depend on the amount applied and the efficiency of application. TABLE 5. Cyhalothrin Residues on Cabbage and Chinese Cabbage, Spain 1 Application Residues (mg/kg) Crop Rate Volume No. Interval last (g a.i./ha) of spray spray to R157836 Rl19321 Total (1/ha) sampling (days) Cyhalothrin Cabbage (wrapper 20 300 4 0 0.03 0.02 0.04 leaves stripped 3 0.01 0.01 0.02 off before 7 0.01 0.01 0.02 analysis) 40 300 4 0 0.02 0.01 0.03 3 0.04 0.02 0.06 7 0.01 0.01 0.01 Chinese cabbage 20 300 4 0 0.44 0.31 0.75 (wrapper leaves 3 0.25 0.18 0.43 not stripped off 7 0.18 0.13 0.32 before analysis) 40 300 4 0 0.58 0.43 1.0 3 0.28 0.21 0.49 7 0.28 0.20 0.48 1 All trials done in 1983 using 10 percent E.C formulation The uses considered by the meeting were on pome fruit and cabbage only. The proposed GAP is up to 100 mg/l (0.01 percent) in the spray, 100 g a.i./ha, per application on apples and pears with a 14-day interval between the last application and harvest. Rates are approximately 10-20 g a.i./ha on cabbage, up to six applications are used with an interval of 2-4 days between the last application and harvesting. The meeting noted that the maximum rate of application used in the trials on pome fruit was not well defined. The highest concentration used was 80 mg/l in the spray; this sprayed to 'run-off' might equate with a high-volume spray of 2 000 l/ha, i.e. 160 g cyhalothrin/ha. Initial deposits from a single application indicated that residues up to 0.5 mg/kg could be expected; this decreased to about 0.2 mg/kg in 14 days. In several other trials, at lower spray concentrations, residues did not exceed 0.2 mg/kg as initial deposits, even when very high volumes were applied and the total rate of application approached 160 g a.i./ha. Although on some occasions some residues diminished with time, several trials showed virtually no decrease in residues over the first 14 days after application. The meeting concluded that more attention should be given to initial deposits and that residues could reach 0.5 mg/kg, especially at the higher rates and volumes needed to control fruit tree red spider mite. This estimate of the maximum residue level was considered suitable for establishing a temporary MRL and should be reviewed in 1986 in the light of more residues data. The data on cabbage were considered inadequate for the estimation of a maximum residue level. RECOMMENDATIONS The following maximum residue level was estimated and recommended as suitable for use as a temporary MRL. Temporary MRL Preharvest interval on which (mg/kg) recommendation is based (days) Pome fruit 0.5 14 FURTHER WORK OR INFORMATION Required (by 1986) 1. Further residues data on pome fruit. 2. Further information on GAP in use of cyhalothrin on pome fruit. REFERENCES Bewick, D.W. & Zinner, C.K.J. Cyhalothrin: degradation in soil. Report 1981 No. RJ 0203B submitted by ICI Plant Protection Division to FAO. (Unpublished) Curl, E.A. & Leahey, J.P. Cyhalothrin: metabolism in cabbage. Report 1983 No. RJ 0308B submitted by ICI Plant Protection Division to FAO. (Unpublished) Hall, J.S. & Leahey, J.P. PP564: degradation on apples. Report No. TMJ 1979 1728B submitted by ICI Plant Protection Division to FAO. (Unpublished) Hall, J.S. & Leahey, J.P. Cyhalothrin: fate in river water. Report No. 1983 RJ 1983 0320B submitted by ICI Plant Protection Division to FAO. (Unpublished) Sapiets, A. Cyhalothrin: residue levels on chinese cabbage from 1983 1984a trials in Spain. Report No. M3749B submitted by ICI Plant Protection Division to FAO. (Unpublished) Sapiets, A. Cyhalothrin: residues on cabbage from 1983 trials in the 1984b United Kingdom. Report No. M3733B submitted by ICI Plant Protection Division to FAO. (Unpublished) Sapiets, A. Cyhalothrin: residue levels on crop samples taken from 1984c trials during 1982-84. Report No. M3791B submitted by ICI Plant Protection Division to FAO. (Unpublished) Sapiets, A. Cyhalothrin: residue levels of the major metabolites in 1984d apple, peach and cabbage from 1982 trials in Japan. Report No. M3708B submitted by ICI Plant Protection Division to FAO. (Unpublished) Sapiets, A. Cyhalothrin: residue levels on cabbage from 1983 trials in 1984e Spain. Report No. M3790B submitted by ICI Plant Protection Division to FAO. (Unpublished) Sapiets, A. & Swaine, H. The determination of residues of cyhalothrin 1983 in crops - a gas-liquid chromatographic method using an internal standard. ICI Plant Protection Division Residue Analytical Method (PPRAM) NO. 70. Stevens, J.E.B. & Poole, N.J. Cyhalothrin: leaching on soil 1981 thick-layer chromatograms. Report No. RJ 0206B submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H. & Sapiets, A. Cyhalothrin: storage stability of frozen crop 1984 samples. Report submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in apples 1983a from Japan in 1982. Residue Data Report No. PP563B003 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in apples 1983b from South Africa in 1982-3. Residue Data Report No. PP563B008 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in apples 1983c from Spain in 1982. Residue Data Report No. PP563B007 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in apples 1983d from UK in 1982. Residue Data Report No. PP563B001 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in pears from 1983e Cyprus in 1982. Residue Data Report No. PP563B005 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in cabbage 1983f from USA in 1982. Residue Data Report No. PP563B006 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in cabbage 1983g from Japan in 1982. Residue Data Report No. PP563B002 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues in peaches 1983h from Japan in 1982. Residue Data Report No. PP563B004 submitted by ICI Plant Protection Division to FAO. (Unpublished) Swaine, H., Sapiets, A. & Burke, S. Cyhalothrin residues n pears 1984 from South Africa in 1982-3. Residue Data Report No. PP563B013 submitted by ICI Plant Protection Division to FAO. (Unpublished)
See Also: Toxicological Abbreviations Cyhalothrin (EHC 99, 1990) Cyhalothrin (ICSC) Cyhalothrin (WHO Food Additives Series 53) Cyhalothrin (WHO Food Additives Series 45) CYHALOTHRIN (JECFA Evaluation) Cyhalothrin (Pesticide residues in food: 1984 evaluations) Cyhalothrin (UKPID)