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    COUMAPHOS                                  JMPR 1972

    Explanation

    This insecticide was previously evaluated in 1968 (FAO/WHO, 1969) at
    which time a temporary acceptable daily intake and temporary
    tolerances were recommended. A requirement for further information was
    indicated on the required rates and frequencies of application,
    preharvest intervals and the resultant residues from countries other
    than U.S.A. and Canada. Since the previous evaluation, additional data
    have become available.

    At the Sixth Session of the Codex Committee on Pesticide Residues it
    was requested that the FAO/WHO Joint Meeting on Pesticide Residues
    evaluate the proposed temporary tolerances in meat, milk and eggs,
    taking into account any new data received.

    RESIDUES IN FOOD AND THEIR EVALUATION

    USE PATTERN

    The compound, as Asuntol WP, is generally applied in a concentration
    of 500 ppm a.i. to domestic animals for the control of ticks and other
    ectoparasites. "Asuntol liquid" (16% and 20% E.C.) is used in
    concentrations of 250 ppm a.i. (maximum). Only in the U.S.A. and
    Canada a concentration of 2 500 ppm is used for the control of some
    pests, including warble fly larvae (Bayer, 1972).

    In countries where one-host ticks occur (i.e., Australia and South
    America), Asuntol WP is usually applied in intervals of 3-4 weeks. In
    Africa the average interval is about 2 weeks. The frequency of
    application of the liquid formulation is identical with that of the
    wettable powder (Bayer, 1972).

    RESIDUES RESULTING FROM SUPERVISED TRIALS

    In animals

    New data was received from the U.S. Department of Agriculture (1972)
    on residues in meat, poultry, eggs and milk arising from carefully
    conducted experiments, at rates required for effective control and
    reflecting good agricultural practice.

    One application of 0.5% dust was applied by hand to individual birds.
    No detectable residues were found in the eggs.

    Residues in poultry meat from this application are shown in Table 1.

    TABLE 1  Coumaphos residues in poultry meat

                                                           

    Days after application             Residues (ppm)
                                                           

    0                                  0.014;0.071;0.063

    3                                  0.044;0.053;0.064

    7                                  0.028;0.035;ND1

    14                                 0.012;0.036;ND

    28                                 0.014;0.012;0.006
                                                           

    1  ND = not detectable.

    In other experiments, when coumaphos was fed to poultry at 40 ppm in
    the feed, residues in heart, liver and gizzard ranged from 0.02 to
    0.06 ppm, while residues in eggs ranged from 0.005 to 0.018 ppm. Dust
    applied at a rate of 1 oz, /bird of 0.5% material gave rise to
    residues in meat of 0.05 to 0.60 ppm and in eggs of 0.020 to 0.058
    ppm.

    Cattle receiving one application of 0.05% spray had no detectable
    residues in meat. However, fat samples had the residues shown in Table
    2.

    TABLE 2  Coumaphos residues in fat of meat of cattle

    Samples stored 6-7 months before analysis

    Days after application                  Residues (ppm)
                                                             
    6                                       0.13
    13                                      0.14
    21                                      0.18
                                                             

    Samples stored 2-5 months before analysis

    Days after application                  Residues (ppm)
                                                             
    7                                       0.12
    14                                      0.06
    25                                      ND1
    28                                      ND
                                                             
    1  ND = not detectable.

    Pigs received six treatments with 0.5% spray at 14-day intervals.
    Samples of muscle taken 8-29 days after last treatment had no
    detectable residues. Samples of fat had residues shown in Table 3.

    TABLE 3  Coumaphos residues in fat of meat of pigs

                                                        

    Days efter last treatment          Residues (ppm)
                                                        

    7                                  0.15
    14                                 0.02
    29                                 ND1
                                                        

    1  ND = not detectable.

    Sheep received six applications of 0.25% spray at 14-day intervals.
    Samples taken after last treatment had the residues shown in Table 4.

    TABLE 4  Coumaphos residues in meat of sheep

                                                                   

    Sample                   Days after last     Residues (ppm)
                             treatment
                                                                   

    Sheep muscle             8                   ND1 to 0.11

                             15                  ND to 0.20

                             29                  ND to 0.12

    Sheep fat                8                   1.3, 1.7, 1.2

                             15                  0.3, 0.4, 0.1

                             29                  0.1, 0.4, 0.2
                                                                   

    1  ND - not detectable.

    Cattle receiving a saturation spray of 1 gallon/animal of 0.5% a.i.
    had residues in meat (steak) samples taken 0 to 3 days after treatment
    of 0.12 to 0.80 ppm. Daily exposure of cattle to 1.0% a.i. in a
    backrubber had residues in meat ranging from not detectable to 0.43
    ppm. Daily exposure to 5% a.i. in a dust bag gave residues in fat of
    0.2 to 0.8 ppm.

    Dairy cows were fed 0-144 ppm coumaphos in their ration for seven
    days. No residues (<0.002 ppm) appeared in the milk at any level of
    coumaphos fed (Miller et al., 1970).

    In supervised trials in Australia (Hurwood, 1966) dairy cattle were
    sprayed with either 0  025% coumaphos or 0.050% coumaphos, and
    excretion in milk was examined in samples taken during a 5-day
    post-treatment period. The results are summarized in Table 5.

    APPRAISAL

    The requirement from the 1968 Joint Meeting for information on the use
    pattern in countries other than Canada and U.S.A. was met with data
    from the manufacturer.

    New data from supervised trials in U.S.A. was received on residues in
    meat, poultry, eggs and milk and from Australia on residues in milk.


    TABLE 5  Residues of coumaphos in butterfat and milk following spray
             treatment of dairy cattle

                                                                         

    Treatment               Time after        Residues (ppm)
                            treatment     Butterfat       Milk
                             (h)
                                                                         

    Before treatment                      N D1            N D

    Coumaphos, emulsion )   5             0.34 - 1.10     0.021 - 0.050
    0.025%              )   21            0.11 - 0.39     0.004 - 0.013
                            29            0.03 - 0.16     0.001 - 0.006
                            45            0.04 - 0.14     0.001 - 0.006
                            53            N.D. - 0.03     N.D.  - 0.001
                            69            N D             N D

    Coumaphos. wettable )   5             0.94 - 1.56     0.028 - 0.051
    powder, 0.050%      )   21            0.59 - 1.14     0.019 - 0.036
                            29            0.28 - 0.57     0.013 - 0.030
                            45            N.D. - 0.21     N.D.  - 0.004
                            53            N D             N D
                                                                         

    1  N D = not detectable (0.005 ppm).


    Poultry receiving dust treatment of 1 ounce/bird of 0.5% a.i. had
    residues of 0.05 to 0.60 ppm in the meat and 0.020 to 0.058 ppm in
    eggs. This "Registered Use" has no time limitation between last
    application and slaughter.

    Cattle receiving a saturation spray of 1 gallon/animal of 0.5% a.i.
    had residues in meat at 0 to 3 days after treatment of 0.12 to 0.80
    ppm. Daily exposure to 5% a.i. in a dust bag resulted in residues in
    fat of 0.2 to 0.8 ppm. These "Registered Uses" have no time limitation
    for slaughter stock.

    Pigs receiving six treatments at 14-day intervals with 0.5% spray had
    no detectable residues in muscle samples taken 8-29 days after last
    treatment. Fat samples had residues of not detectable to 0.15 ppm.

    Dairy cattle receiving a single spray treatment of either 0.025%
    emulsion or 0.050% wettable powder had residues in butterfat of 0.03
    to 0.16 ppm from the first treatment and 0.20 to 0.57 ppm from the
    second treatment at 29 hours after the treatment.

    Sheep receiving six applications at 14-day intervals of 0.25% spray
    had residues in muscle samples taken 8-29 days after last treatment of
    not detectable to 0.20 ppm. Fat samples ranged from 1.7 ppm at 8 days
    to 0.4 at 15 days to 0.1 ppm at 29 days. For this rate and manner of
    application to sheep, the "Registered Use" reads: "Do not apply within
    15 days of slaughter".

    On the basis of the new data summarized above and taking into account
    the time limitations applicable to the types of treatment employed, a
    need for revision of the previously recommended tolerances was
    indicated.

    RECOMMENDATIONS

    TEMPORARY TOLERANCES

    The temporary tolerances recommended in 1968 for meat including
    poultry (fat basis) are replaced by the following revised and
    additional/temporary tolerances. The tolerance figures include the
    oxygen analogue.

                                                 ppm

         Poultry, fat of meat of cattle          1

         Fat of meat of sheep, pigs, goats
         milk and milk products (fat basis)      0.5

         Eggs (shell free basis)                 0.05

    TEMPORARY ADI

    The data on which the temporary ADI for coumaphos were based were
    re-evaluated and it was decided to extend the temporary ADI for a
    further three-year period.

    FURTHER WORK OR INFORMATION

         None

    REFERENCES

    Bayer, A.G. (1972) Personal communication.

    FAO/WHO. (1969) 1968 evaluations of some pesticide residues in food.
    FAO/PL: 1968/M/9/1; WHO/Food Add./69.35.

    Miller, R.W., Gordon, C.H., Morgan, N.O., Bowman, M.C. and Morton
    Beroya. (1970) Coumaphos as a feed additive for the control of
    house-fly larvae in manure. J. Econ. Entomol., 63(3): 853-855.

    U.S. Department of Agriculture. (1972) Data submitted to the Joint
    Meeting.

    Hurwood, I.S. (1966) Studies on pesticide residues. 1. Excretion of
    pesticides in milk following dermal treatment of dairy cattle with
    coumaphos and dioxathion. Bulletin No. 120, Queensland Department of
    Primary Industries, Division of Animal Industry.
    


    See Also:
       Toxicological Abbreviations
       Coumaphos (ICSC)
       Coumaphos (FAO/PL:1968/M/9/1)
       Coumaphos (WHO Pesticide Residues Series 5)
       Coumaphos (Pesticide residues in food: 1978 evaluations)
       Coumaphos (Pesticide residues in food: 1980 evaluations)
       Coumaphos (Pesticide residues in food: 1983 evaluations)
       Coumaphos (Pesticide residues in food: 1990 evaluations Toxicology)