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    CHLORDANE        JMPR 1974

    Explanation

         Chlordane was evaluated in the Joint Meetings of 1963, 1965, 1967
    and 1970 (FAO/WHO, 1964, 1965, 1968 and 1971). Recommended tolerances
    for numerous fruits, vegetables, grains and animal products are at
    various steps of the Codex Committee on Pesticide Residues (CCPR)
    procedure (Alinorm 74/24, Appendix II). The recommended tolerances are
    expressed as combined cis- and trans-chlordane for commodities of
    plant origin and as combined cis- and trans-chlordane and oxychlordane
    for animal products.

         The 1974 Meeting of the CCPR has returned items 12.15 to 12.31 to
    step 6 and requested the 1974 Joint Meeting to review new data
    requested from governments. The purpose of the request was to
    determine whether the recommended maximum limits of 0.3 and 0.2 mg/kg
    for potatoes (12.15) through collards (12.31) are necessary if only
    the alpha (cis)-chlordane and gamma (trans)-chlordane are to be
    measured.

    RESIDUES IN FOOD AND THEIR EVALUATION

    RESIDUES RESULTING FROM SUPERVISED TRIALS

         New data from member governments have not been made available to
    the 1974 Joint Meeting. Velsicol Chemical Corporation has submitted
    new data on the levels and identity of chlordane residues in potatoes,
    beets, asparagus, turnips, parsnips, swiss chard, pumpkin, mustard
    greens, spinach, and cucumbers. All data were from supervised trials
    reflecting registered uses in the USA. US uses are generally
    restricted to applications before edible parts form or to soil
    treatments.

         Eight principal components of chlordane residues have been
    identified in the studies and their relative abundance measured after
    various periods of weathering. The residues are heptachlor, heptachlor
    epoxide, alpha (cis)-chlordane, gamma (trans)-chlordane, nonachlor,
    photo-alpha-chlordane, and "compounds C and E" which are two isomers
    of chlordane. Not all of the compounds were found in all samples. The
    additional metabolite oxychlordane is found only in animal tissues.
    Certain other constituents have been postulated but were not detected.
    The distribution of residues at any time is complex but the alpha -
    and gamma - isomers still appear to be the best index of chlordane
    residues at the point of regulation (though not always the most
    abundant single component).

         The highest residue levels occur in the root crops, and may
    occasionally exceed 0.3 mg/kg when total residues are calculated. If
    only alpha- and gamma-chlordane are measured, the residues usually do
    not exceed 0.2 mg/kg. In the other vegetables studied, the total
    residues are usually between 0.1 and 0.2 mg/kg. If only alpha- and

    gamma-chlordane are measured, the residues do not exceed 0.1 mg/kg.
    The supervised residue trials in the USA would thus tend to support
    somewhat lower tolerance levels than the presently recommended
    tolerances for combined alpha- and gamma-chlordane residues in the
    vegetables 12.15 through 12.31. Lower tolerance levels however would
    leave little latitude for the normal variations observed in crops, and
    particularly in crops from countries other than the USA for which no
    data are available.

    METHODS OF RESIDUE ANALYSIS

         The principal advance in the technology since 1970 is apparently
    the availability of pure reference standards for the individual
    constituents. The analytical methods employed in the Velsicol study
    were not identified. Presumably they are Velsicol methods AM 0507 and
    AM 0508 which were discussed in the JMPR 1970 report referred to
    above. These methods, based on electron-capture gas chromatography,
    determine the unchanged components of technical chlordane plus their
    photolytic and metabolic conversion products. There is no single GLC
    column which will provide resolution of all constituents and it is
    unlikely that these methods would be suitable for regulatory purposes.
    For this reason the Joint Meeting reaffirms the desirability of
    regulating chlordane residues through measurement of the alpha- and
    gamma-chlordane residues in plants. Satisfactory methods are available
    for determination of these two components (FAO/WHO, 1971).

    APPRAISAL

         The 1974 Meeting of the CCPR has returned the recommended
    chlordane tolerances for potatoes (12.15) through collards (12.31) to
    step 6 and requested governments to submit new data for the purpose of
    showing whether maximum limits of 0.3 and 0.2 mg/kg are required when
    the tolerances are expressed as combined alpha (cis)-chlordane and
    gamma (trans)-chlordane. Data were made available to the Joint Meeting
    by the Velsicol Chemical Corporation on the identity and levels of
    chlordane residue components in various root crops and vegetables from
    US trails. While the data generally show that the recommended
    tolerances are slightly higher than residues reported in the
    supervised trials, the Joint Meeting was unable to conclude that there
    was sufficient latitude to reduce the recommended tolerances.

         The meeting reaffirms the desirability of expressing chlordane
    maximum limits for plant products as the combined residues of alpha
    (cis)-chlordane and gamma (trans)-chlordane.

    FURTHER WORK OR INFORMATION

    DESIRABLE

    1.   Information from Governments on use patterns.

    2.   Information from countries other than the USA on residues
    resulting from approved uses.

    REFERENCES

    FAO/WHO (1964). Evaluation of the toxicity of pesticide residues in
    food. FAO/PL/M/63/13; WHO/Food Add./23.

    FAO/WHO (1965). Evaluation of the toxicity of pesticide residues in
    food. FAO/PL/1965/10/1; WHO/Food Add./27.65.

    FAO/WHO (1968). 1967 Evaluations of some pesticide residues in food.
    FAO/PL/1967/M/11/1; WHO/Food Add./68.30.

    FAO/WHO (1971). 1970 Evaluations of some pesticide residues in food.
    FAO/AGP/1970/M/12/1; WHO/Food Add./71.42.
    


    See Also:
       Toxicological Abbreviations
       Chlordane (EHC 34, 1984)
       Chlordane (HSG 13, 1988)
       Chlordane (PIM 574)
       Chlordane (FAO Meeting Report PL/1965/10/1)
       Chlordane (FAO/PL:1967/M/11/1)
       Chlordane (FAO/PL:1969/M/17/1)
       Chlordane (AGP:1970/M/12/1)
       Chlordane (WHO Pesticide Residues Series 2)
       Chlordane (Pesticide residues in food: 1977 evaluations)
       Chlordane (Pesticide residues in food: 1982 evaluations)
       Chlordane (Pesticide residues in food: 1984 evaluations)
       Chlordane (Pesticide residues in food: 1986 evaluations Part II Toxicology)