FAO/PL:1967/M/11/1 WHO/Food Add./68.30 1967 EVALUATIONS OF SOME PESTICIDE RESIDUES IN FOOD THE MONOGRAPHS The content of this document is the result of the deliberations of the Joint Meeting of the FAO Working Party of Experts and the WHO Expert Committee on Pesticide Residues, which met in Rome, 4 - 11 December, 1967. (FAO/WHO, 1968) FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS WORLD HEALTH ORGANIZATION Rome, 1968 DIMETHOATE IDENTITY Chemical names 0,0-dimethyl S-(N-methylcarbamoylmethyl) phosphorodithioate; S-methylcarbamoylmethyl 0,0-dimethyl phosphorodithioate; 0,0-dimethyl S-alpha-mercapto-N-methylacetamido dithiophosphate; N-monoethylamide of 0,0-dimethyldithiophosphoryl acetic acid; methyl dimethyldithiophosphoryl acetamide. Synonyms Rogor, Fortion MM Empirical formula C5H12NO3PS2 Structural formula S " (CH3O)2 - P - S -CH2-CO - NHCH3 Other relevant chemical properties Technical dimethoate - 93.3 per cent EVALUATION FOR ACCEPTABLE DAILY INTAKES Biochemical aspects Dimethoate is a cholinesterase inhibitor. The molar concentration of the pure compound necessary to produce 50 per cent cholinesterase inhibition in the rat brain in vitro (I50) is 8.5 × 10-3. It decomposes to give products which are more toxic than the original substance (Casida & Sanderson, 1963). Various studies (O'Brien, 1959; O'Brien, 1961); Sanderson & Edson, 1964) carried out with dimethoate labelled with 32P have shown that there is rapid absorption from the digestive tract. The radioactivity is concentrated in the liver, bile, kidneys and urine. There is no accumulation in the fat depots. Elimination is rapid in the rat and in man, 76-90 per cent of the radioactivity being found in the urine after 24 hours. In the guinea-pig, 25-40 per cent of the radioactivity is recovered in the faeces. Four dimethoate metabolites with anticholinesterase activity (molar I50's in 30 minutes at 37° in rat brain; 4.7 × 10-6; 1.1 × 10-5; approximately 0.2 × 10-5 and approximately 0.1 × 10-5) have been identified in the rat and in man. One of them seems to be a product resulting from thiono-oxidation, leading to the formation of the oxygen homologue of dimethoate and followed by hydrolysis with production of a thiocarboxyl derivative which constitutes the chief metabolite of dimethoate in mammals. In vitro studies on human liver enzymes indicated that dimethoate could inhibit the non-specific esterases to a greater degree than acetylesterase (Ecobichon & Kalow, 1963). For further information see In animals. Acute toxicity Dimethoate LD50 Animal Route (mg/kg body-weight) Reference Pure Laboratory Technical grade Mouse, female Oral 60 - 60 Sanderson & Edson, 1964 Rat, male Oral 500-600 280-350 180-325 " Rat, female Oral 570-680 300-356 240-336 " Rat, male Intraperitoneal - 175-325 - " Rat, female Intraperitoneal - 350 - " Rat, male Intravenous - 450 - " Hamster, male Oral - 200 - " Guinea-pig Oral 550 600 350-400 " Rabbit Oral 500 450 approx. 300 " Hen Oral 50 40 approx. 30 " The rat oral LD50's (mg/kg body weight) of desmethyl oxy-carboxy dimethoate and oxycarboxy dimethoate have been determined to be <600 and <800 respectively whereas the hen oral LD50 of oxygen analog of dimethoate has been determined to be 100 (Levinskas and Shaffer, 1965). Acute oral toxicity of dimethoate was not potentiated by any of 17 other insecticides (Sanderson & Edson, 1964). Short-term studies Mouse. A three generation reproduction study was conducted at dietary levels of 0, 5, 15 and 50 ppm of dimethoate, with two litters produced per generation. Second litter animals were used for composing succeeding generations. No effect of the compound was seen in fertility, lactation or survival of the pups to weaning, gross appearance of all pups produced, weights of major organs of F2b animals and gross and microscopic appearance of tissues of the F3b animals, autopsied at 21 days of age (Ribelin et al., 1965). Rat. Groups of 10 male rats were fed diets containing 1, 5, 25 and 125 ppm of dimethoate for 15 weeks. At the highest concentration, a slight fall in the rate of gain of weight was observed as well as mild symptoms of poisoning (slight muscular fibrillation). In the group fed 25 ppm and higher concentrations, a significant fall in the cholinesterase activity of the plasma and erythrocytes was observed, while in the animals fed 5 ppm a fall of 20 per cent in cholinesterase activity was found. At 1 ppm there was no effect on the cholinesterase activity of the plasma, erythrocytes or brain (Edson & Noakes, 1960). Young rats in groups of 20 fed diets containing 2, 8 and 32 ppm of dimethoate for 90 days and other groups of 20 rats fed 50, 100 and 200 ppm for 35 days showed no haematological abnormalities, nor any significant histopathological changes. Regarding the cholinesterase activity of the plasma and the erythrocytes, the highest dose which did not give a significant inhibition was 32 ppm of dimethoate (West et al., 1961). Groups of 20 male rats were maintained for 6-12 months on diets containing various concentrations of laboratory grade dimethoate. At 800 ppm severe intoxication developed within a few days; the chemical was withdrawn after a week and complete recovery occurred in 10-14 days. No toxic effects were seen at 50 ppm or below. Marked inhibition of erythrocyte cholinesterase activity occurred at 50 ppm but at 10 ppm and below neither erythrocyte nor plasma cholinesterase showed significant inhibition throughout the test. At the end of the experiment there were no gross or microscopic changes in any group attributable to dimethoate. The maximum no-effect level in these experiments corresponded to 0.5-0.8 mg/kg body-weight per day. In the same experiment, further groups of 20 weanling male rats were treated for 5-1/2 months at dose levels of 5, 10 and 20 ppm of dimethoate. The maximum no-effect level in these experiments was 5 ppm corresponding to 0.3-0.6 mg/kg body-weight per day. A further test with the commercial liquid formulation of dimethoate on similar groups of male and female rats lasted 12 weeks. The maximum no-effect level in these experiments was again 5 ppm corresponding to 0.4-0.6 mg/kg body-weight per day (Sanderson & Edson, 1964). Groups of 10-15 males and 10-15 females were fed 0, 330, 1000 and 3000 ppm of oxy-carboxy dimethoate for 33 days. At 3000 ppm, rate of weight gain, food consumption and food utilization were affected in both sexes, and kidney weight ratios were slightly elevated in the males. Erythrocytic cholinesterase activity was inhibited in relation to dose in both sexes at 1000 and 3000 ppm, and slightly inhibited in the females at 330 ppm. Brain cholinesterase activity was significantly affected in the 3000 ppm females. Plasma cholinesterase was unaffected at all levels. No effect was seen on mortality, behaviour and gross and microscopic appearance of major organs (Levinskas & Sheffer, 1965). Initial groups of 25 males and 25 females were fed 0, 0.2, 0.4, 0.8 and 1.6 ppm at the oxygen analog of dimethoate for 28 days. Animals from each group were selected for determination of cholinesterase activities at 3, 7, 14 and 28 days. At 8 ppm, there was a marked effect on erythrocyte, plasma (except in the females) and brain cholinesterase activities. At 1.6 ppm, erythrocyte cholinesterase activity was affected in both sexes and brain cholinesterase activity in the females at 28 days. No distinct effect on cholinesterase was found at lower levels. No effect at any level was found on food intake, rate of weight gain, and gross and microscopic appearance of major organs (Fogleman et al., 1965). Guinea-pig. Groups of guinea-pigs were fed for 3 weeks on lettuce and brassica leaves that had been treated with dimethoate and contained residues of up to 189 ppm. No toxic effects were seen, and the cholinesterase inhibition observed was in agreement with that in parallel groups given daily oral doses of the same quantity of laboratory grade dimethoate (Sanderson & Edson, 1964). Chicken. In laying hens, dimethoate given over a period of 59 weeks at a concentration of 30 ppm daily in the drinking-water caused inhibition of plasma cholinesterase and some reduction in appetite, but no egg abnormalities (Sherman, et al., 1963). Groups of 6 hens were fed 0, 65, 130 and 260 ppm of dimethoate for 4 weeks. There was no effect on mortality, but hens at the high level were unable to maintain their original weights. Groups of 6 hens received 0 and 130 ppm of dimethoate and 2000 and 4000 ppm of tri-o-cresyl phosphate for 4 weeks. At the termination of the study, samples of brain, thoracic cord and sciatic nerve were examined for effects on axons and myelin sheaths. Tri-o-cresyl phosphate was found to produce a demonstrable general myelin loss, but dimethoate had no effect on either sheaths or axons. (Levinskas and Shaffer, 1965). Groups of 6 hens were fed 0, 60, 120 and 240 ppm of oxygen analog of dimethoate for 4 weeks. At the termination of the study, samples of brain, thoracic cord and sciatic nerve were examined for effects on axons and myelin sheaths. No effect was found on nerve tissue or on survival, but it was noted that the test birds were generally unable to maintain body weight satisfactorily (Levinskas and Shaffer, 1965). Dog. Three groups each of 4 dogs, 2 males and 2 females, were fed diets containing 2, 10 and 50 ppm for 13 weeks. No significant harmful effect was noted. The cholinesterase activity of the erythrocytes was only slightly decreased at the highest concentration of 50 ppm while that of the plasma was unaffected at any of the concentrations employed (West et al., 1961). Man. Twenty subjects ingested 2.5 mg of dimethoate in aqueous solution, corresponding to about 0.04 mg/kg body-weight daily for 4 weeks. No toxic effect was observed, nor any significant change in the blood cholinesterase activity. The same results were found in 2 subjects who ingested daily during 21 days, 9 mg (0.13 mg/kg) and 18 mg (0.26 mg/kg body-weight) dimethoate respectively (Sanderson and Edson, 1964). Thirty-six male and female volunteers were given daily oral doses of dimethoate of 5, 15, 30, 45 and 60 mg for periods of 14 to 57 days. There was no effect on the blood cholinesterase levels with intakes of 5 and 15 mg daily, but there was at 30 mg and above (Edson et al., 1967). Long-term studies No data available. Comments The findings now available from man provide a satisfactory basis for assessment. The reproduction studies now completed in the mouse meet the needs of the request previously made for such experiments in the rat. TOXICOLOGICAL EVALUATION Level causing no significant toxicological effect Man 0.2 mg/kg body-weight/day. Estimate of acceptable daily intake for man 0.02 mg/kg body-weight. EVALUATION FOR TOLERANCES USE PATTERN Pre-harvest treatments Dimethoate is a systemic insecticide used on a number of fruit and vegetable crops to combat aphids, leafhoppers, leafminers, lygus bugs, pear psylla, various mites (except rust mites), olive flies, various citrus pests and fruit eating larvae. Typical maximum recommended usages are presented in the following Table I. TABLE I Crop Dosage, lbs Pre-harvest actual period, days apples - pears 0.5 lbs/100 gal 28 beans 0.25 - 0.5 lbs/A 0* peas 0.2 lbs/A 0 brassica 0.25 - 0.5 lbs/A 7 cabbage 0.25 - 0.5 lbs/A 3 leafy vegetable 0.25 lbs/A 14 * Cattle should not be allowed to graze on vines Dimethoate is also used on alfalfa, wheat, safflower and pea forage against aphids, leafhoppers, lygus bugs, grasshoppers and thrips with applications of 0.25 - 0.5 lb/100 gal and a pre-grazing and pre-harvest period of 21 - 28 days. Post-harvest treatments No post harvest use of dimethoate is known. Other uses Dimethoate is used in formulations on a large number of ornamental plants, shrubs and trees. It has been recommended for control of houseflies and maggots and is used for this purpose around the home, barn, and commercial establishments with a caution to avoid contamination of human and animal food supplies. RESIDUES RESULTING FROM SUPERVISED TRIALS Numerous supervised field trials have been made on a large number of crops under varying cultural and climatic conditions with different rates of application and harvest times. Much of this data has been submitted to the U.S. Food and Drug Administration in the form of petitions. Table II summarizes data representative of residues found when the maximum recommended dosage of pesticide (see Table I) was used. TABLE II Crop Typical initial Pre-harvest Residue at end Estimated residues, ppm period, days of pre-harvest half-life period, ppm. days Fruits Apples 4.0 - 6.0 28 1.0 - 1.5 15 Pears 1.0 - 2.0 28 0.4 10 Cole crops Cauliflower 5.0 - 6.0 7 0.6 - 0.9 3 Broccoli 9.0 - 23.0 7 2.0 2 Cabbage 3.0 - 8.0 3 2.0 1-4 Head lettuce 5.0 7 1.2 4 Leafy vegetables Spinach 4.0 - 12.0 14 0 - 0.3 2-3 Kale 5.0 14 0.1 1 Turnip and mustard greens 7.0 - 9.0 14 0.3 2 Leaf lettuce 0.2 - 0.9 14 0.1 - 0.3 4 Collards 1.0 - 10.0 14 0.1 - 0.5 3 Endive 2.0 - 3.0 14 0.1 2 TABLE II (cont'd) Crop Typical initial Pre-harvest Residue at end Estimated residues, ppm period, days of pre-harvest half-life period, ppm. days Escarole and chard 1.0 - 2.0 14 0.1 3 Legumes (fruit and pod only) Snap beans 1.5 0 1.5 4 Green beans 0.5 - 1.0 0 0.5 - 1.0 7 Lima beans 0.4 0 0.4 1 Peas 1.0 0 1.0 7 Other vegetables Peppers 0.1 - 0.3 0 0.1 - 0.3 7 Tomatoes 0.2 - 0.8 7 0.2 4 TABLE III Crop Dosage Typical Pre-harvest Residue at and Estimated lbs actual initial period of pre-harvest half-life per 100 gal residues ppm days period, ppm days Peaches 0.25 lb 3.0-7.0 14 1.0-1.5 7 Apricots 0.12 lb 8.0-10.0 14 <1.0 5-7 Cherries 0.2 lb 2.0-12.0 14 1.0-1.5 3-5 Grapes 0.5 lb 7.0-10.0 28 <1.0 8 Strawberries 0.4 lb 7 0.3 Grapefruit 0.8 lb 2.0 90 1.2 120 Oranges (peeled) 0.8 lb <1.0 90 <0.5 100 Lemons 1.0 lb 21 0.1 Tangerines 0.5 lb 48 0.1 Artichokes 0.25-4.0 lb 7 1.1 Brussels sprouts 1.5 lb 12 1.1 Potatoes 0.4-1.0 lb 10-126 0.1 Sugar beats 0.4-2.0 lb 48-100 <0.1-0.2 Wheat 0.3 lb 58 0.2 Corn 0.5-1.0 lb 21 0.1 TABLE III (cont'd) Crop Dosage Typical Pre-harvest Residue at and Estimated lbs actual initial period of pre-harvest half-life per 100 gal residues ppm days period, ppm days Olives (eating) 0.16 lb 2.0-4.0 30 0.6 12 Olive oil 0.5 lb 2.0-3.0 14 <1.0 10 A number of studies made throughout the world are also reported in a review by de Pietri-Tonelli et al., (1965). Table III summarizes the results of these studies on crops not listed in Table II. FATE OF RESIDUES General considerations Extensive studies have been made on the metabolism of dimethoate in both plants and mammals. The major route in mammals appears to be through the thiocarboxy derivative to the corresponding dimethyl esters of phosphoric, thiophosphoric or dithiophosphoric acids. A side pathway may also occur through the thiodesmethyl carboxy derivative. The oxygen analog of dimethoate forms in animals and although this route may be considered minor with respect to the principle route, it is an important one to consider because of the toxicity of the oxygen analog. Evidence indicates that in plants the formation of the oxygen analog is a major route. However, various investigators are not in agreement regarding the quantitative measure of the material degraded by this oxidation route, nor are they in agreement on the other pathways of metabolism involving hydrolysis and demethylation which occur to a substantial degree. See Figure I for the proposed schemes. In soils The fate of dimethoate in soils is pertinent to this presentation since the compound is systemic from soils; that is, it is absorbed by plants from the soil and transported to the aerial part of the plant in sufficient quantity to make the whole plant insecticidal to certain insects. The persistency of dimethoate in sandy loam soil was determined by Bohn (1964). The biological half-life was found to be 4 days under drought conditions and shortened to 2-1/2 days with moderate rainfall. Parker and Dewey (1965) applied a dosage 5 times that used by Bohn to gravelly silt loam. Bioassay with vinegar flies showed a rapid decline of dimethoate in the first few days with 40 per cent remaining on the 5th day. The rate of dissipation then slowed down considerably with approximately 20 per cent remaining after 54 days. In plants Santi and de Pietri-Tonelli (1959b) showed the systemic nature of dimethoate. Application of the insecticide and its oxygen analog to roots, stems and leaves of bean plants resulted in translocation to other parts of the plant. Foliar spray on trees resulted in residues in the fruit. Both compounds penetrate from the outside to the inside of the fruit and kill fruit-eating larvae. They showed that transformation to the oxygen analog occurs in broad bean plants after root absorption of the insecticide and in cherries picked from sprayed trees.Dauterman et al. (1960) studied the metabolism of dimethoate-32P on the surface and inside cotton, corn, pea and potato leaves following foliar application. Substantial quantities of oxygen analog were found in all samples. Although the quantity decreased with time, there was a proportionate increase in the oxygen analog relative to dimethoate. Internally this is attributed to enzymatic oxidation; however, since this same oxidation results on non-biological surfaces exposed to the atmosphere, non-enzymatic oxidation may occur on the leaf surface. Based on the quantities of water-soluble metabolites on the surface and inside leaves, Dauterman postulates that different mechanisms of degradation occur. There also appears to be a marked difference in crop species. Plant-surface water-soluble metabolites were primarily the oxycarboxy derivative; whereas the desmethyl derivatives were the major internal metabolites. Pea plants did not follow the pattern of cotton, corn and potato plants, but had about 50 per cent residue of phosphoric acid both on the surface and inside leaves. No dithiophosphoric acids or thiocarboxy derivative were found in any samples. Apparently oxidation occurs more rapidly than hydrolysis. Hacskaylo and Bull (1963) found that metabolism of dimethoate in excised cotton leaves resembles the pathway proposed for mammals and is different from that occurring from foliar treatments. They found the level of oxygen analog fairly constant and always less than 6 per cent of the total metabolites found. Recently Lucier (1967) studied the metabolism of 32P and 14C-carbonyl labeled dimethoate in bean plants using four modes of application: foliar treatment, stem injection, root absorption and excised leaves. Conversion of dimethoate to the oxygen analog was the major route of degradation and was directly correlated with the ease of translocation of dimethoate to the leaf tissue. Thus the amount of oxygen analog was greatest for the excised leaves and less for the foliar application. In root absorption studies, the oxygen analog content was 4-1/2 times the amount of dimethoate and represented 10 per cent of the administered dose 10 days after application. The major hydrolysis products were the thiocarboxy derivative, dimethyl phosphorothioate and dimethyl phosphorodithioate. Only trace quantities of the thiodesmethyl carboxy compound were found. A new compound, des-N-methyl dimethoate was detected in trace quantities in all samples. Foliar application gave rise to large quantities of two unknown metabolites which were postulated to be the N-hydroxy methyl derivatives of dimethoate and its oxygen analog. These unknowns are rapidly degraded and not detectable after four days. Chillwell and Beecham (1960) found that climatic conditions had no substantial effect on residue level of dimethoate if similar applications were made on crops at the same growth stage. Recent preliminary investigations by Watts and Storherr (1967) show that residues on field-sprayed kale may contain five times as much oxygen analog as the parent compound 14 days after treatment. The oxygen analog seems to be the only metabolite of toxicological importance. Therefore, its residue level in food products must be considered. Data submitted to the U.S. Food and Drug Administration and data reported in the literature by Santi* (1961), Santi and Giacomelli (1962) and Dauterman et al. (1960) indicate that the amount of oxygen analog is generally not above 10-20 per cent of the total toxic residue. Results in olives are an exception where the oxygen analog was found as high as 67 per cent of the total residue after 30 days. In animals Early studies by Roberts at al (1958) and Kaplanis et al (1959) revealed that dimethoate was absorbed rapidly into the blood of cattle and was converted to a metabolite several times more toxic than the parent compound as determined by enzymatic analysis and bioassay. This compound and many of the major urinary metabolites were not identified. Santi and de Pietri-Tonelli (1959a) showed that dimethoate converted chemically and in vitro by incubation in rat liver slices to the oxygen analog. An unusually high rate of degradation of dimethoate in sheep liver to the thiocarboxy derivative was found by Uchida et al, (1964). This was attributed to amidase activity. Sanderson and Edson (1964) found that dimethoate behaved as a typical indirect anticholinesterase agent by conversion in the liver to at least four short lived metabolites whose hydrolysis products are rapidly excreted mainly in the urine. Oral administration of dimethoate-32P at dosages of 100 mg/kg to rats and 10 to 40 mg/kg to lactating cows led Dauterman and co-workers (1959) to conclude that the metabolic pathway was similar for both species, and that dimethoate was attacked hydrolytically at five sites on the molecule. Only small quantities of labelled material were found in the feces. Dimethoate was rapidly absorbed into the blood and rapidly excreted in the urine with 75 per cent of the administered dose eliminated within 24 hours primarily as the thiocarboxy derivative. As the quantity of the thiocarboxy derivative and dimethylthiophosphoric acid decreased, the level of dimethylphosphoric acid increased and appeared relatively stable since conversion to simpler phosphoric acids was very slow. When the desmethyl derivative was fed to rats, recovery of better the 85 per cent of unchanged chemical in the urine occurred suggesting that this is not the major metabolic route in animals. Analysis of blood samples indicated that a maximum of chloroform solubles was reached at two hours with 1.5 ppm dimethoate equivalents present in cows which were, treated with 10 g/kg. The level of cholinesterase was severely depressed for the animal treated at 40 mg/kg but no depression occurred at the lower feeding level. * As quoted by de Pietri-Tonelli et al (1965) A composite sample representing total milk secreted over a 288 hour period showed that 0.0068 per cent of the administered dose was eliminated in this manner. The amount of chloroform solubles decreased rapidly with less than 0.02 ppm dimethoate equivalents present after 48 hours from the oral dose of 10 mg/kg and 0.04 ppm from the 40 mg/kg dose. Chloroform solubles in the fat were less than 0.1 ppm after eight hours. The only significant quantity of residue in bovine tissues was found in the liver with a maximum of 0.23 ppm of chloroform solubles at the lower feeding level. Chamberlain et al. (1961) investigated the metabolism of dimethoate by intramuscular administration and oral feeding of sheep at 10 and 40 mg/kg. He too found that only a small per cent of the labeled dose was excreted in the faeces and that over 80 per cent of the radioactive material was eliminated in the urine by 72 hours with the thiocarboxy derivative being the major component. As the thiocarboxy content decreased there was an increase in dimethyl phosphoric acid and an unknown which he tentatively identified as the desmethyl carboxy derivative and suggested that it formed from the thiocarboxy compound. Trace quantities of dimethoate and its oxygen analog were present in all sheep urine samples. Analysis of the blood samples showed that the amount of organo-solubles decreased very rapidly with less than 0.1 per cent remaining after 12 hours. Ninety per cent of the organosoluble residue vas dimethoate or its oxygen analog. The proportion of oxygen analog to dimethoate increased as the quantity of dimethoate decreased. In storage and processing No commercial use of dimethoate for storage products is known. Rowland (1966) studied the metabolism in stored wheat and sorghum grains fortified at 2 and 10 parts per million. Trace amounts of dimethoate and its oxygen analog were found after 4 and 7 days' storage. No active anti-cholinesterase components were found at subsequent samplings. No hydrolytic products of the oxygen analog were found, but significant quantities of dimethoate hydrolysis products were detected by paper chromatography. These included the thiocarboxy derivative, the thiodesmethyl and thiodesmethyl carboxy derivative, and the dimethyl esters of thio and dithiophosphoric acid. METHODS OF RESIDUE ANALYSIS The major metabolic pathway in plants indicates that the oxygen analog of dimethoate is a significant metabolite. A minor pathway in animals yields the same compound. Any adequate method for residue analysis must take this compound into account. Most of the data presented in Table II was obtained from one of two methods of analysis. The first method of Waldron (1962) (adapted for apples from a method described by George (1962) for dimethoate in alfalfa) determines only the parent compound, dimethoate. The pesticide is extracted with methylene chloride, followed by alkaline hydrolysis, and colorimetric determination of the resultant methylamine by reaction with 1-chloro-2,4-dinitrobenzene. Numerous tests were made to show that the method in specific for dimethoate and that some 28 commonly used pesticides do not interfere. Steller and Curry developed a method incorporating a thin layer chromatographic isolation of dimethoate and its oxygen analog and a total phosphorous determination of the separate fractions measuring a molybdenum blue complex. This method is specific for dimethoate and its oxygen analog. Although recoveries were lower than for the Waldron method, they were consistent and the authors claim a sensitivity of 0.06 ppm. The data in Table II were obtained by a variety of methods. Chillwell and Beecham (1960) macerated plant tissues with water acidified with acetic acid and extracted the aqueous phase with chloroform. Microdistillation was used to purify the extracted insecticide and phosphorous was determined colorimetrically after an acid digestion. Santi and de Pietri-Tonelli (1959), referred to by de Pietri-Tonelli et al (1965), used a chloroform extraction with isolation by paper chromatography and determination of phosphorous with a molybdic reagent and 2, 6-dibromo-N-chloro-p-quinoneimine and subsequent spraying with propylene glycol. Van Middlem and Waites (1964) compared a dinitrochlorobenzene colorimetric procedure with an electron capture gas chromatography procedure for dimethoate. Recoveries were 71 per cent for the colorimetric procedure and about 85 per cent for the gas chromatography procedure. Correcting for recoveries the results of the two procedures were in agreement. In both procedures methylene chloride was used to extract the pesticide. The organic phase was further cleaned with activated carbon and the aqueous phase was passed through a polyethylene-coated alumina column prior to colorimetric determination. For the gas chromatographic analysis benzene was added to the extract before evaporation of the methylene chloride. These investigators reported that the oxygen analog is infinitely more soluble in water than in organic solvents. Unlike dimethoate which has a partition coefficient of 20 for chloroform and water, the oxygen analog coefficient is only 0.7. Watts and Storherr (1967) found a much higher ratio of oxygen analog in residues using an ethyl acetate extraction with a nuchar carbon cleanup and a gas chromatography thermionic detection. Since the oxygen analog in so highly water-soluble and favors the aqueous phase in many partitioning systems, care must be exercised in choosing any method of analysis to ensure that the more polar oxygen analog is not lost. It is difficult to evaluate fully the quantitative data published on metabolism without knowledge of the exact conditions of the experiments. It is hoped that improved methodology will assist in clarifying some apparent anomalies. NATIONAL TOLERANCES Country Tolerances, ppm Product United States of America 2 apples, pears 2 18 vegetables Canada 2 apples, pears Benelux 0.5 fruits and vegetables Federal Republic of Germany 0.5 fruits and vegetables Australia 2.0 RECOMMENDATIONS FOR TOLERANCES Temporary tolerances until 3 December 1970 When dimethoate is utilized in accordance with good agricultural practice to protect food products, when necessary against insect infestation, the treated product may have residues as high as those shown below (total of dimethoate and oxygen analog): Tree fruits (including citrus) 2 ppm Vegetables (excluding tomatoes and 2 ppm peppers) Tomatoes and peppers 1 ppm By no means will all samples of fruits and vegetables contain this amount of residue; in fact, only a small, yet unknown portion of each product in these categories is likely to be treated. There are some data available on the disappearance of this compound during storage of treated wheat. The nature of this disappearance simulates that which occurs in living plants. The compound is systemic; it can be assumed that residues will not be reduced materially during washing and peeling. Since the compound is not recommended, generally, for use on a large variety of food products, it is recommended that the above levels be adopted on a temporary basis until further data are made available on losses during cooking and data are acquired from total diet studies. The proposed temporary tolerance is for a combination of dimethoate and its oxygen analog. FURTHER WORK Further work required before 30 June 1970 1. Studies on the fate of the compound during processing and preparation for consumption. 2. Amount of residue appearing in total diet studies. Further work desirable Worldwide use data. REFERENCES PERTINENT TO EVALUATION FOR ACCEPTABLE DAILY INTAKES Casida, J.E. and Sanderson, D.M. (1962) Nature, 189, 507. Casida, J.E. and Sanderson, D.M. (1963) J. Agr. Food Chem., 2, 91. Ecobichon, D.J. and Kalow, W. (1963) Canad. J. Biochem., 41, 1537 Edson, E.F. and Noakes, D.N. (1960) Toxicol. appl. Pharmacol., 2, 523 Edson, E.F., Jones, K.H. and Watson, W.A. (1967) Brit. med. J., ii, 554 Fogleman, R.W., Levinskas, G.J. and Shaffer, C.B. (1965) Unpublished reports submitted by American Cyanamid Company. Levinskas, G.J. and Shaffer, C.B. (1965) Unpublished reports submitted by American Cyanamid Company. O'Brien, R.D. (1959) Nature, 183, 121 O'Brien, R.D. (1961) Biochem. J., 79, 229 Ribelin, W.B., Levinskas, G.J. and Shaffer, C.B. (1965) Unpublisbed reports submitted by American Cyanamid Company Sanderson, D.M. and Edson, E.F. (1964) Brit. J. industr. Med., 21, 52 Sherman, M., Ross, E., Sanchet, F.F. and Chang, M.T.Y. (1963) J. econ. Ent., 56, 10 West, B., Vidone, L.B. and Shaffer, C.B. (1961) Toxicol. appl. Pharmacol., 3, 210 REFERENCES PERTINENT TO EVALUATION FOR TOLERANCES American Cyanamid Co. (1962-7) Pesticide petitions submitted to the U.S. Food and Drug Administration. Bohn, W.R. (1964) The disappearance of dimethoate from soil. J. Econ. Ent. 57: 798-9. Chamberlain, W.R., Gatterdam, P.E., and Hopkins, D.E. (1961) The metabolism of p32-labelled dimethoate in sheep. J. Econ. Ent. 54 (4) : 733-40. Chillwell, E.D. and Beecham, P.T. (1960) Residues of 0,0-dimethyl S-(N-methyl-carbamoyl-methyl) Phosphorothiolothionate (dimethoate) in sprayed crops. J. Sci. Fd. Agric. 11 (7) : 400-7. Dauterman, W.C., Casida, J.E., Knaak, J.B. and Kowalczyk, T. J. (1959) Agr. Food Chem. 7 (3) : 188-93. Dauterman, W.C., Biado, G.B., Casida, J.E. and O'Brien, R.D. J. Agr. Food Chem. 1960 8 (2) a 115-19. de Pietri-Tonelli, F. and Barontini, A. (1959) Analisi per via biologica dei residue di Rogor in campioni di carciofi ed indivia. Internal report of Montecatini. de Pietri-Tonelli, P., Bazzi, B. and Santi, R. (1965) Rogor (dimethoate) residues in food crops. Residue Reviews. Vol 11. 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See Also: Toxicological Abbreviations Dimethoate (EHC 90, 1989) Dimethoate (HSG 20, 1988) Dimethoate (ICSC) Dimethoate (FAO Meeting Report PL/1965/10/1) Dimethoate (FAO/PL:CP/15) Dimethoate (JMPR Evaluations 2003 Part II Toxicological) Dimethoate (AGP:1970/M/12/1) Dimethoate (Pesticide residues in food: 1983 evaluations) Dimethoate (Pesticide residues in food: 1984 evaluations) Dimethoate (Pesticide residues in food: 1984 evaluations) Dimethoate (Pesticide residues in food: 1987 evaluations Part II Toxicology) Dimethoate (Pesticide residues in food: 1996 evaluations Part II Toxicological)